{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,23]],"date-time":"2026-06-23T11:48:47Z","timestamp":1782215327395,"version":"3.54.5"},"reference-count":20,"publisher":"Termedia Sp. z.o.o.","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pol J Pathol"],"abstract":"<jats:p>Cutaneous carcinoma is one of\u00a0the\u00a0most common neoplasm tumors in the\u00a0West. Its incidence rate is one of\u00a0the\u00a0fastest growing tumors in China. The\u00a0Kr\u00fcppel-like factor 6 (KLF6) is a\u00a0latent tumor suppressor. Decreased KLF6 is related to the\u00a0occurrence and progression of\u00a0many cancers in human. Our previous studies have demonstrated that KLF6 was down-regulation in cutaneous malignant melanoma (CMM), and was significant correlated with ulcer, lymph node metastasis and clinical stage, suggesting that KLF6 loss is expected to become a\u00a0biological indicator of\u00a0poor prognosis in CMM patients. In this research, we would further study the\u00a0features of\u00a0KLF6 in the\u00a0malignant progression of\u00a0CMM. The\u00a0expression of\u00a0KLF6 was up-regulated by lentivirus infection containing KLF6, and short hairpin RNA (shRNA) was used for knockdown of\u00a0KLF6 in CMM cells. Western blot, RT-qpcr, CCK8 assay, transwell migration assays, wound healing assay and flow cytometry were used to test the\u00a0role of\u00a0KLF6 in the\u00a0CMM. We found that reduced expression of\u00a0KLF6 significantly enhanced proliferation, migration and invasion. Moreover, KLF6 induced CMM cell apoptosis and G1 cycle arrest. The\u00a0decreased KLF6 expression is expected to be a\u00a0biological indicator of\u00a0poor prognosis in CMM patients.<\/jats:p>","DOI":"10.5114\/pjp.2023.132224","type":"journal-article","created":{"date-parts":[[2023,10,25]],"date-time":"2023-10-25T03:32:41Z","timestamp":1698204761000},"page":"194-202","source":"Crossref","is-referenced-by-count":0,"title":["The Kr\u00fcppel-like factor 6 represses proliferation and invasion of\u00a0cutaneous malignant melanoma"],"prefix":"10.5114","volume":"74","author":[{"given":"Yuan-Yuan","family":"Li","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qi-Ming","family":"Zheng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nan","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Min","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"3061","published-online":{"date-parts":[[2023,10,25]]},"reference":[{"key":"ref-1691784","doi-asserted-by":"crossref","unstructured":"Cummins DL, Cummins JM, Pantle H, et al. Cutaneous malignant melanoma. Mayo Clin Proc 2006; 81: 500-507.","DOI":"10.4065\/81.4.500"},{"key":"ref-1691785","doi-asserted-by":"crossref","unstructured":"Reeves HL, Narla G, Ogunbiyi O, et al. Kruppel-like factor 6 (KLF6) is a\u00a0tumor-suppressor gene frequently inactivated in colorectal cancer.\u00a0Gastroenterology 2004; 126: 1090-1103.","DOI":"10.1053\/j.gastro.2004.01.005"},{"key":"ref-1691786","doi-asserted-by":"crossref","unstructured":"Camacho-Vanegas O, Narla G, Teixeira MS, et al. Functional inactivation of\u00a0the\u00a0KLF6 tumor suppressor gene by loss of\u00a0heterozygosity and increased alternative splicing in glioblastoma.\u00a0Int J Cancer 2007; 121: 1390-1395.","DOI":"10.1002\/ijc.22809"},{"key":"ref-1691787","doi-asserted-by":"crossref","unstructured":"Kremer-Tal S, Narla G, Chen Y, et al. Downregulation of\u00a0KLF6 is an\u00a0early event in hepatocarcinogenesis, and stimulates proliferation while reducing differentiation.\u00a0J Hepatol 2007; 46: 645-654.","DOI":"10.1016\/j.jhep.2006.10.012"},{"key":"ref-1691788","doi-asserted-by":"crossref","unstructured":"Cho YG, Kim CJ, Park CH, et al. Genetic alterations of\u00a0the\u00a0KLF6 gene in gastric cancer.\u00a0Oncogene 2005; 24: 4588-4590.","DOI":"10.1038\/sj.onc.1208670"},{"key":"ref-1691789","doi-asserted-by":"crossref","unstructured":"Yamashita K, Upadhyay S, Osada M, et al. Pharmacologic unmasking of\u00a0epigenetically silenced tumor suppressor genes in esophageal squamous cell carcinoma.\u00a0Cancer Cell 2002; 2: 485-495.","DOI":"10.1016\/S1535-6108(02)00215-5"},{"key":"ref-1691790","doi-asserted-by":"crossref","unstructured":"DiFeo A, Martignetti JA, Narla G. The\u00a0role of\u00a0KLF6 and its splice variants in cancer therapy. Drug Resist Update 2009; 12: 1-7.","DOI":"10.1016\/j.drup.2008.11.001"},{"key":"ref-1691791","doi-asserted-by":"crossref","unstructured":"Narla G, Heath KE, Reeves HL, et al. KLF6, a\u00a0candidate tumor suppressor gene mutated in prostate cancer.\u00a0Science 2001; 294: 2563-2566.","DOI":"10.1126\/science.1066326"},{"key":"ref-1691792","doi-asserted-by":"crossref","unstructured":"Kettunen E, Anttila S, Sepp\u00e4nen JK, et al. Differentially expressed genes in nonsmall cell lung cancer: expression profiling of\u00a0cancer-related genes in squamous cell lung cancer.\u00a0Cancer Genet Cytogenet 2004; 149: 98-106.","DOI":"10.1016\/S0165-4608(03)00300-5"},{"key":"ref-1691793","doi-asserted-by":"crossref","unstructured":"Teixeira MS, Camacho-Vanegas O, Fernandez Y, et al. KLF6 allelic loss is associated with tumor recurrence and markedly decreased survival in head and neck squamous cell carcinoma.\u00a0Int J Cancer 2007; 121: 1976-1983.","DOI":"10.1002\/ijc.22926"},{"key":"ref-1691794","doi-asserted-by":"crossref","unstructured":"Hartel M, Narla G, Wente MN, et al. Increased alternative splicing of\u00a0the\u00a0KLF6 tumour suppressor gene correlates with prognosis and tumour grade in patients with pancreatic cancer.\u00a0Eur J Cancer 2008; 44: 1895-1903.","DOI":"10.1016\/j.ejca.2008.06.030"},{"key":"ref-1691795","doi-asserted-by":"crossref","unstructured":"Cummins DL, Cummins JM, Pantle H, et al. Cutaneous malignant melanoma.\u00a0Mayo Clin Proc 2006; 81: 500-507.","DOI":"10.4065\/81.4.500"},{"key":"ref-1691796","doi-asserted-by":"crossref","unstructured":"Chen C, Hyytinen ER, Sun X, et al. Deletion, mutation, and loss of\u00a0expression of\u00a0KLF6 in human prostate cancer.\u00a0Am J Pathol 2003; 162: 1349-1354.","DOI":"10.1016\/S0002-9440(10)63930-2"},{"key":"ref-1691797","doi-asserted-by":"crossref","unstructured":"Benzeno S, Narla G, Allina J, et al. Cyclin-dependent kinase inhibition by the\u00a0KLF6 tumor suppressor protein through interaction with cyclin D1.\u00a0Cancer Res 2004; 64: 3885-3891.","DOI":"10.1158\/0008-5472.CAN-03-2818"},{"key":"ref-1691798","doi-asserted-by":"crossref","unstructured":"Slavin DA, Koritschoner NP, Prieto CC, et al. A\u00a0new role for the\u00a0Kruppel-like transcription factor KLF6 as an\u00a0inhibitor of\u00a0c-Jun proto-oncoprotein function.\u00a0Oncogene 2004; 23: 8196-8205.","DOI":"10.1038\/sj.onc.1208020"},{"key":"ref-1691799","doi-asserted-by":"crossref","unstructured":"Ito G, Uchiyama M, Kondo M, et al. Kr\u00fcppel-like factor 6 is frequently down-regulated and induces apoptosis in non-small cell lung cancer cells.\u00a0Cancer Res 2004; 64: 3838-3843.","DOI":"10.1158\/0008-5472.CAN-04-0185"},{"key":"ref-1691800","doi-asserted-by":"crossref","unstructured":"Glinsky GV, Glinskii AB, Stephenson AJ, et al. Gene expression profiling predicts clinical outcome of\u00a0prostate cancer. J Clin Invest 2004; 113: 913-923.","DOI":"10.1172\/JCI20032"},{"key":"ref-1691801","doi-asserted-by":"crossref","unstructured":"Zhang N, Yan QQ, Lu L, et al. The\u00a0KLF6 splice variant KLF6-SV1 promotes proliferation and invasion of\u00a0non-small cell lung cancer by up-regulating PI3K-AKT signaling pathway.\u00a0J Cancer 2019; 10: 5324-5331.","DOI":"10.7150\/jca.34212"},{"key":"ref-1691802","doi-asserted-by":"crossref","unstructured":"Zhang N, Li Z, Xiao W, et al. KLF6-SV1 is a\u00a0new prognostic biomarker in postoperative patients with non-small cell lung cancer.\u00a0Cancer Manag Res 2018; 10: 3937-3944.","DOI":"10.2147\/CMAR.S171805"},{"key":"ref-1691803","doi-asserted-by":"crossref","unstructured":"Zhang N, Qiu LY, Yang F, et al. Clinical and prognostic significance of\u00a0Kr\u00fcppel-like transcription factor 6 expression in 67 patients with cutaneous malignant melanoma in China. Pol J Pathol 2021; 72: 245-251.","DOI":"10.5114\/pjp.2021.111775"}],"container-title":["Polish Journal of Pathology"],"original-title":[],"link":[{"URL":"https:\/\/www.polishjournalofpathology.com\/pdf-170082-136607","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.polishjournalofpathology.com\/pdf-170082-136607","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,6,23]],"date-time":"2026-06-23T10:58:36Z","timestamp":1782212316000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.polishjournalofpathology.com\/The-Kruppel-like-factor-6-represses-proliferation-and-invasion-of-cutaneous-malignant,170082,0,2.html"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,10,25]]},"references-count":20,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2023]]}},"alternative-id":["170082"],"URL":"https:\/\/doi.org\/10.5114\/pjp.2023.132224","relation":{},"ISSN":["1233-9687","2084-9869"],"issn-type":[{"value":"1233-9687","type":"print"},{"value":"2084-9869","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,10,25]]}}}