{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,11]],"date-time":"2026-10-11T12:26:11Z","timestamp":1791721571476,"version":"4.3.4"},"reference-count":25,"publisher":"Termedia Sp. z.o.o.","issue":"1","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pol J Pathol"],"abstract":"<jats:p>This study aimed to investigate whether the crosstalk between the osteoprotegerin (OPG)\/receptor activator of nuclear factor-kB (RANK)\/receptor activator of nuclear factor-kB ligand (RANKL) in bone marrow mesenchymal stem cells (BMSCs) and Wnt\/b-catenin pathways in Pca cells regulates bone metastasis of PCa.&lt;br \/&gt;\nOur study showed that there was increased OPG\/RANKL\/RANK and b-catenin expression in the tissue of PCa and its bone metastasis. This study further showed that RANKL knockdown in BMSCs or b-catenin knockdown in PC-3s blocked the proliferation and migration of BMSCs and the proliferation, migration, and invasion of PC-3s in vitro. Conversely, RANKL overexpression in BMSCs and b-catenin overexpression in PC-3s promoted the proliferation and migration of BMSCs and the proliferation, migration, and invasion of PC-3s in vitro. These data indicate that the RANKL pathway in BMSCs promoted the PC-3s invasion and the catenin pathway in PC-3s activated BMSCs with expression of cancer-associated fibroblast markers, which promoted the bone metastasis. &lt;br \/&gt;\nThis suggests that the interaction and crosstalk between BMSCs in bone microenvironment and PCa play a critical role in the exquisite tropism for Pca bone metastasis. Cancer therapies classically target tumour cells; however, based on this study, targeting BMSCs in bone microenvironment is a reasonable option for PCa therapy strategy.<\/jats:p>","DOI":"10.5114\/pjp.2025.150029","type":"journal-article","created":{"date-parts":[[2025,5,6]],"date-time":"2025-05-06T03:12:07Z","timestamp":1746501127000},"page":"25-37","source":"Crossref","is-referenced-by-count":2,"title":["Crosstalk between OPG\/RANKL\/RANK in bone marrow mesenchymal stem cells and Wnt\/b-catenin pathway in prostate cancer cells regulates bone metastasis of\u00a0prostate cancer"],"prefix":"10.5114","volume":"76","author":[{"given":"Shihua","family":"Ye","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiongyun","family":"Lin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meihua","family":"Deng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shulong","family":"Huang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Changlin","family":"Mao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiabin","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wuming","family":"Zhan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guangbing","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"3061","published-online":{"date-parts":[[2025,4,28]]},"reference":[{"key":"ref-1688407","doi-asserted-by":"crossref","unstructured":"Pernar CH, Ebot EM, Wilson KM, Mucci LA. 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