{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,23]],"date-time":"2026-06-23T11:51:01Z","timestamp":1782215461919,"version":"3.54.5"},"reference-count":40,"publisher":"Termedia Sp. z.o.o.","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pol J Pathol"],"abstract":"<jats:p>Colon adenocarcinoma (COAD) is one of\u00a0the\u00a0most prevalent forms of\u00a0cancer in the\u00a0world. Still, the\u00a0molecular mechanism of\u00a0COAD development remains unknown, making it especially important to investigate the\u00a0molecular mechanism of\u00a0COAD development and identify new therapeutic targets. &lt;br \/&gt;\nA\u00a0real-time fluorescence quantification polymerase chain reaction (RT-qPCR) was used to determine the\u00a0level of\u00a0miR-21-5p expression\u00a0in COAD tissues and cell lines. Both miR-21-5p silencing and overexpression were performed in LOVO and T84 cell lines. Cell viability, apoptosis rate, migration, and invasion ability were determined using MTT, flow cytometry, and the\u00a0Transwell assay, respectively. Western blot was applied to detect the\u00a0levels of\u00a0protein expression associated with the\u00a0epithelial-mesenchymal transition (EMT). &lt;br \/&gt;\nUsing a\u00a0dual luciferase reporter gene, the\u00a0targeting connection among miR-21-5p and Tensin 1 was validated. Tensin 1 expression was silenced to investigate its  effect on miR-21-5p inhibitor activity in COAD cells. Subcutaneous tumor-forming animal experiments in nude mice were used to investigate the\u00a0effect of\u00a0miR-21-5p on COAD tumor growth in vivo. Ki-67 expression was identified through immunohistochemistry. MiR-21-5p was found in high concentrations in COAD tissues and cells. Overexpression of\u00a0miR-21-5p increased COAD cell line viability and EMT, facilitated cell migration and invasion, and inhibited apoptosis. Tensin 1 was regulated negatively by miR-21-5p. Tensin 1 silencing reversed the\u00a0effect of\u00a0miR-21-5p silencing on COAD cells. Subcutaneous tumor formation experiments in nude mice revealed that inhibiting miR-21-5p expression slowed  the growth rate of tumor volume. &lt;br \/&gt;\nAccording to immunohistochemical results, the\u00a0percentage of\u00a0Ki-67-positive cells was significantly lower in the\u00a0anti-miR-21-5p group. MiR-21-5p levels were upregulated in COAD cells, and reducing miR-21-5p expression inhibited COAD cell viability, migration, invasion, and EMT in vitro. Tensin 1 negatively regulated miR-21-5p, which regulated COAD cell and EMT progression.<\/jats:p>","DOI":"10.5114\/pjp.2025.156556","type":"journal-article","created":{"date-parts":[[2025,11,28]],"date-time":"2025-11-28T09:24:18Z","timestamp":1764321858000},"page":"214-232","source":"Crossref","is-referenced-by-count":0,"title":["MiR-21-5p regulates colon adenocarcinoma cell progression and epithelial-mesenchymal transition  by negatively regulating Tensin 1"],"prefix":"10.5114","volume":"76","author":[{"given":"Lingqing","family":"Cai","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiangrong","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chuanqing","family":"Ke","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Baiping","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuqing","family":"Xiong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huiying","family":"Fu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"3061","published-online":{"date-parts":[[2025,11,28]]},"reference":[{"key":"ref-1687783","doi-asserted-by":"crossref","unstructured":"Labianca R, Beretta GD, Kildani B, et al. 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