{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,11]],"date-time":"2026-05-11T07:07:54Z","timestamp":1778483274488,"version":"3.51.4"},"reference-count":28,"publisher":"Termedia Sp. z.o.o.","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Reumatologia"],"abstract":"<jats:sec>\n                    <jats:title>Introduction<\/jats:title>\n                    <jats:p>Idiopathic inflammatory myopathies (IIM) are a\u00a0heterogeneous group of\u00a0autoimmune diseases characterized by muscle inflammation and systemic involvement. The\u00a0study aimed to eva\u00adluate the\u00a0immunophenotypic profile of\u00a0circulating lymphocytes in patients with IIM and investigate its association with clinical manifestations, serological profiles, treatment regimens, and disease outcomes.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Material and methods<\/jats:title>\n                    <jats:p>This single-center, cross-sectional study included 40 patients with IIM and 5 age- and sex-matched healthy controls. Peripheral blood samples were collected and processed to isolate peripheral blood mononuclear cells using the\u00a0density gradient centrifugation method. Flow cytometry analysis was performed to phenotype lymphocyte subpopulations, including T cells (CD3+CD4+ and CD3+CD8+), B cells (CD3\u2013CD19+), and natural killer (NK) cells (CD3\u2013CD16+CD56+). Statistical analy\u00adsis was conducted to compare lymphocyte profiles between the\u00a0study and control groups and to assess correlations with clinical features, serological markers, and treatment regimens.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>The\u00a0IIM group had a\u00a0mean age of\u00a058.55 \u00b112.70 years, with a\u00a0predominance of\u00a0females (60%). The\u00a0most common IIM subtypes were antisynthetase syndrome (35%) and dermatomyositis (30%). No significant differences in the\u00a0profiles of\u00a0circulating lymphocytes were found between IIM patients and healthy controls. Patients with cardiac involvement had significantly higher proportions of\u00a0double-positive T cells (p = 0.0035), while those with cutaneous ulcers and dysphagia showed significantly lower proportions of\u00a0CD4+CD8+ T cells. Natural killer cells were significantly lower in patients with anti-Mi2 antibodies (p = 0.0155) but higher in anti-Ro52 positive patients (p = 0.0150). In contrast, B cells were notably higher in patients with anti-Mi2 (p = 0.0182) and lower in individuals with anti-Ro52 (p = 0.0124). Patients with anti-Ro52 positivity were also characterized by higher proportions of\u00a0double-negative T cells (p = 0.0465). Treatment regimens did not impact the\u00a0profiles of\u00a0circulating lymphocytes.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Patients with IIM exhibit distinct alterations in lymphocyte subpopulations, with specific immune cell profiles associated with clinical phenotypes and serological profiles.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.5114\/reum\/213516","type":"journal-article","created":{"date-parts":[[2026,5,11]],"date-time":"2026-05-11T06:08:09Z","timestamp":1778479689000},"page":"94-104","source":"Crossref","is-referenced-by-count":0,"title":["Distinct profiles of\u00a0circulating lymphocytes reflect clinical and serological variations in idiopathic inflammatory myopathies"],"prefix":"10.5114","volume":"64","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7881-5731","authenticated-orcid":true,"given":"Aleksandra","family":"Opinc-Rosiak","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7343-8551","authenticated-orcid":true,"given":"Joanna","family":"Sarnik","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2036-375X","authenticated-orcid":false,"given":"Joanna","family":"Makowska","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"3061","published-online":{"date-parts":[[2026,4,30]]},"reference":[{"key":"ref-1855756","doi-asserted-by":"crossref","unstructured":"Lundberg IE, Fujimoto M, Vencovsky J, et al. 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