{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,11]],"date-time":"2026-07-11T18:04:40Z","timestamp":1783793080434,"version":"3.55.0"},"reference-count":0,"publisher":"Termedia Sp. z.o.o.","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Reumatologia"],"published-print":{"date-parts":[[2015,5,4]]},"abstract":"<jats:p>&lt;b&gt;Objectives&lt;\/b&gt;: The presence of human leukocyte antigen B27 (HLA-B27) is strongly associated with ankylosing spondylitis. HLA-B27 testing is routinely applied in the diagnosis of this disease. The aim of the present study was to compare two methods of HLA-B27 detection \u2013 a genetic sequence-based method and a flow cytometry assay.&lt;br \/&gt;\n&lt;b&gt;Material and methods&lt;\/b&gt;: Peripheral blood was obtained from 300 individuals with suspected spondyloarthropathy. Expression of HLA-B27 on the T cell surface was analysed by flow cytometry assay using GS145.2 monoclonal antibody specific for HLA-B27. DNA was isolated from the whole blood. Genes coding for HLA-B27, -B40 and -B47:01 were detected by polymerase chain reaction using the MW02\/MW09 primer pair. Then, positive samples were sequenced in order to discriminate allelic variations of the HLA-B27 gene. Results of sequencing were analysed using Chromas LITE 2.1.1 software, BLAST software and the IMGT\/HLA database. Ambiguous samples were additionally analysed by polymerase chain reaction using E91 and E136 primers amplifying a 135-bp fragment of the human HLA-B27 gene.&lt;br \/&gt;\n&lt;b&gt;Results&lt;\/b&gt;: Among 300 samples, 76 were HLA-B27-positive on the basis of flow cytometry analysis. Genetic sequence analysis confirmed positivity of 73 from among 76 samples. Two hundred twenty six samples were HLA-B27-negative, whereas the result of one sample analysis was ambiguous. Fifty-three samples were identified as allelic variation 27:05, 19 samples as allelic variation 27:02, and one sample as allelic variation 27:07.&lt;br \/&gt;\n&lt;b&gt;Conclusions&lt;\/b&gt;: This study shows that the genetic sequence-based method and the flow cytometry assay give consistent results in 99% of cases. The performed genetic analysis proves that the majority of HLA-B27-positive samples belong to the 27:05 allelic variation, which is strongly associated with high risk of ankylosing spondylitis.<\/jats:p>","DOI":"10.5114\/reum.2015.51506","type":"journal-article","created":{"date-parts":[[2015,6,24]],"date-time":"2015-06-24T07:53:03Z","timestamp":1435132383000},"page":"74-78","source":"Crossref","is-referenced-by-count":15,"title":["HLA-B27 detection \u2013 comparison of genetic sequence-based method and flow cytometry assay"],"prefix":"10.5114","volume":"53","author":[{"given":"Urszula","family":"Skalska","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna","family":"Kozakiewicz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"W\u0142odzimierz","family":"Ma\u015bli\u0144ski","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Monika","family":"Jurkowska","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"3061","published-online":{"date-parts":[[2015,5,18]]},"container-title":["Rheumatology"],"original-title":["Oznaczanie antygenu HLA-B27 \u2013 por\u00f3wnanie metody opartej na sekwencjonowaniu DNA z metod\u0105 cytometryczn\u0105"],"link":[{"URL":"https:\/\/reu.termedia.pl\/pdf-57281-86468","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/reu.termedia.pl\/pdf-57281-86468","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,1,30]],"date-time":"2023-01-30T20:59:41Z","timestamp":1675112381000},"score":1,"resource":{"primary":{"URL":"https:\/\/reu.termedia.pl\/HLA-B27-detection-comparison-of-genetic-sequence-based-method-and-flow-cytometry,57281,0,2.html"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,5,4]]},"references-count":0,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2015]]}},"alternative-id":["57281"],"URL":"https:\/\/doi.org\/10.5114\/reum.2015.51506","relation":{},"ISSN":["0034-6233","2084-9834"],"issn-type":[{"value":"0034-6233","type":"print"},{"value":"2084-9834","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,5,4]]}}}