{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,3]],"date-time":"2026-11-03T17:42:51Z","timestamp":1793727771923,"version":"4.6.1"},"reference-count":42,"publisher":"Termedia Sp. z.o.o.","issue":"1","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Reumatologia"],"published-print":{"date-parts":[[2021,3,5]]},"abstract":"<jats:sec><jats:title>Introduction<\/jats:title><jats:p>T-cell-mediated adaptive immunity contributes to the development and persistence of\nankylosing spondylitis (AS). Mesenchymal stromal\/stem cells (MSCs) have immunomodulatory potential and are able to inhibit T-cell proliferation, but their functionality in AS patients is relatively\nunknown. The aim of the study was to assess the direct anti-proliferative effects of MSCs isolated\nfrom subcutaneous abdominal adipose tissue of AS patients (AS\/ASCs) on allogeneic T lymphocytes, using commercially available ASC lines from healthy donors (HD\/ASCs) as a control.<\/jats:p><\/jats:sec><jats:sec><jats:title>Material and methods<\/jats:title><jats:p>CD3+CD4+ T-cells were isolated from peripheral blood of healthy blood donors, activated with anti-CD3\/CD28 beads, and co-cultured for 5 days with untreated and TNF+IFN-\u03b3\npre-stimulated HD\/ASCs (5 cell lines) and AS\/ASCs, obtained from 11 patients (6F\/5M). The proliferative response of T-cells was analysed by flow cytometry, while the concentrations of kynurenines,\nprostaglandin E2 (PGE-2), interleukin 10 (IL-10), and interleukin 1 receptor antagonist (IL-1Ra) were\nmeasured spectrophotometrically or using a specific enzyme-linked immunosorbent assay (ELISA).<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>HD\/ASCs and AS\/ASCs similarly reduced the T-cell proliferation response, i.e. the percentage of proliferating cells, the proliferation, and replication indices, and these effects were dependent\nmostly on soluble factors. In the co-cultures of activated CD4+ T-cells with HD\/ASCs and AS\/ASCs\nsignificant increases of kynurenines, PGE-2, and IL-1Ra, but not IL-10, production were observed.\nThe release of these factors was dependent either on cell-to-cell contact (IL-10, IL-1Ra) or soluble\nfactors (kynurenines, PGE-2). There was a moderate to strong negative correlation between T-cell\nproliferative response, and the concentrations of kynurenines, PGE-2, and IL-10, but not IL-1Ra. This\nassociation was more evident in the case of TI-treated AS\/ASCs than HD\/ASCs.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>AS\/ASCs, similar to HD\/ASCs, exert a direct effective anti-proliferative impact on CD4+\nT cells, acting via soluble factors that are released in cell contact-dependent (IL-10) and independent\n(kynurenines, PGE-2) pathways. Thus, our results suggest that AS\/ASCs are potentially useful for\ntherapeutic application.<\/jats:p><\/jats:sec>","DOI":"10.5114\/reum.2021.103940","type":"journal-article","created":{"date-parts":[[2021,3,10]],"date-time":"2021-03-10T13:31:31Z","timestamp":1615383091000},"page":"12-22","source":"Crossref","is-referenced-by-count":5,"title":["Direct anti-proliferative effect of adipose-derived mesenchymal stem cells of ankylosing spondylitis patients on allogenic CD4+ cells"],"prefix":"10.5114","volume":"59","author":[{"given":"Ewa","family":"Kuca-Warnawin","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Magdalena","family":"Pleba\u0144czyk","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Krzysztof","family":"Bonek","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ewa","family":"Kontny","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"3061","published-online":{"date-parts":[[2021,2,28]]},"reference":[{"key":"ref-781884","doi-asserted-by":"publisher","DOI":"10.5114\/reum.2014.44704"},{"key":"ref-781885","doi-asserted-by":"publisher","DOI":"10.5114\/reum.2014.42795"},{"key":"ref-781886","doi-asserted-by":"publisher","DOI":"10.1172\/JCI125212"},{"key":"ref-781887","doi-asserted-by":"publisher","DOI":"10.1136\/ard.59.2.135"},{"key":"ref-781888","doi-asserted-by":"publisher","DOI":"10.1002\/art.1780380407"},{"key":"ref-781889","first-page":"1962","article-title":"CD8+ T-cells oligoclonally expanded in synovial fluid at onset of spondyloarthropathy selectively proliferate in response to self-antigens: characterization of cell specificities in nonclonal populations","volume":"31","author":"Trevi\u0144o MA","year":"2004","unstructured":"Trevi\u0144o MA, Teixeiro E, Bragado R. (2004). CD8+ T-cells oligoclonally expanded in synovial fluid at onset of spondyloarthropathy selectively proliferate in response to self-antigens: characterization of cell specificities in nonclonal populations J Rheumatol. 31: 1962-1972.","journal-title":"J Rheumatol"},{"key":"ref-781890","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1002653"},{"key":"ref-781891","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1202926"},{"key":"ref-781892","first-page":"903","article-title":"Combined manual and automated immunophenotypisation identified disease-specific peripheral blood immune subpopulations in rheumatoid arthritis, ankylosing spondylitis and psoriatic arthritis","volume":"38","author":"\u0160u\u0107ur A","year":"2020","unstructured":"\u0160u\u0107ur A, Jaji\u0107 Z, Iki\u0107 Matija\u0161evi\u0107 M, et al. (2020). Combined manual and automated immunophenotypisation identified disease-specific peripheral blood immune subpopulations in rheumatoid arthritis, ankylosing spondylitis and psoriatic arthritis Clin Exp Rheumatol. 38: 903-916.","journal-title":"Clin Exp Rheumatol"},{"key":"ref-781893","doi-asserted-by":"publisher","DOI":"10.15436\/2471-0598.17.022"},{"key":"ref-781894","doi-asserted-by":"publisher","DOI":"10.1186\/scrt75"},{"key":"ref-781895","doi-asserted-by":"publisher","DOI":"10.2147\/JIR.S256932"},{"key":"ref-781896","doi-asserted-by":"publisher","DOI":"10.1155\/2014\/913050"},{"key":"ref-781897","doi-asserted-by":"publisher","DOI":"10.1007\/s00109-016-1489-x"},{"key":"ref-781898","doi-asserted-by":"publisher","DOI":"10.1186\/ar3257"},{"key":"ref-781899","doi-asserted-by":"publisher","DOI":"10.3390\/cells8121659"},{"key":"ref-781900","doi-asserted-by":"publisher","DOI":"10.1136\/ard.2009.108233"},{"key":"ref-781901","doi-asserted-by":"publisher","DOI":"10.1099\/rheumatology\/kes129"},{"key":"ref-781902","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.a.21010"},{"key":"ref-781903","first-page":"4839","article-title":"Human adipose tissue-derived stem cells inhibit T-cell lymphoma growth in vitro and in vivo","volume":"34","author":"Ahn JO","year":"2014","unstructured":"Ahn JO, Chae JS, Coh YR, et al. 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Human adipose tissue-derived stem cells inhibit T-cell lymphoma growth in vitro and in vivo Anticancer Res. 34: 4839-4847.","journal-title":"Anticancer Res"},{"key":"ref-781904","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2014.01.008"},{"key":"ref-781905","doi-asserted-by":"publisher","DOI":"10.1016\/j.cellimm.2010.06.006"},{"key":"ref-781906","doi-asserted-by":"publisher","DOI":"10.1111\/cei.12423"},{"key":"ref-781907","doi-asserted-by":"publisher","DOI":"10.1016\/j.yexcr.2010.08.008"},{"key":"ref-781908","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1002239"},{"key":"ref-781909","doi-asserted-by":"publisher","DOI":"10.1016\/j.yexcr.2010.04.018"},{"key":"ref-781910","doi-asserted-by":"publisher","DOI":"10.1186\/s13256-018-1776-y"},{"key":"ref-781911","doi-asserted-by":"publisher","DOI":"10.1177\/0963689720945682"},{"key":"ref-781912","doi-asserted-by":"publisher","DOI":"10.1038\/cddis.2015.327"},{"key":"ref-781913","doi-asserted-by":"publisher","DOI":"10.3390\/jcm8071025"},{"key":"ref-781914","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcyt.2014.07.004"},{"key":"ref-781915","doi-asserted-by":"publisher","DOI":"10.1155\/2019\/2107414"},{"key":"ref-781916","doi-asserted-by":"publisher","DOI":"10.1089\/ten.TEA.2008.0630"},{"key":"ref-781917","doi-asserted-by":"publisher","DOI":"10.3727\/000000006780666957"},{"key":"ref-781918","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.170.12.5809"},{"key":"ref-781919","doi-asserted-by":"publisher","DOI":"10.1016.\/j.yexcr.2010.08.008"},{"key":"ref-781920","doi-asserted-by":"publisher","DOI":"10.1007\/s00702-011-0681-y"},{"key":"ref-781921","doi-asserted-by":"publisher","DOI":"10.1038\/icb.2011.75"},{"key":"ref-781922","doi-asserted-by":"publisher","DOI":"10.1002\/1521-4141(200006)30:6<1683::AID-IMMU1683>3.0.CO;2-A"},{"key":"ref-781923","doi-asserted-by":"publisher","DOI":"10.3390\/ijms20153827"},{"key":"ref-781924","doi-asserted-by":"publisher","DOI":"10.1016\/j.smim.2019.101335"},{"key":"ref-781925","doi-asserted-by":"publisher","DOI":"10.1002\/stem.2254"}],"container-title":["Rheumatology"],"original-title":[],"link":[{"URL":"https:\/\/reu.termedia.pl\/pdf-132423-85964","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/reu.termedia.pl\/pdf-132423-85964","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,1,27]],"date-time":"2023-01-27T08:01:02Z","timestamp":1674806462000},"score":1,"resource":{"primary":{"URL":"https:\/\/reu.termedia.pl\/Direct-anti-proliferative-effect-of-adipose-derived-mesenchymal-stem-cells-of-ankylosing,132423,0,2.html"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,2,28]]},"references-count":42,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2021]]}},"alternative-id":["132423"],"URL":"https:\/\/doi.org\/10.5114\/reum.2021.103940","relation":{},"ISSN":["0034-6233","2084-9834"],"issn-type":[{"value":"0034-6233","type":"print"},{"value":"2084-9834","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,2,28]]}}}