{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,1,28]],"date-time":"2023-01-28T06:18:53Z","timestamp":1674886733859},"reference-count":22,"publisher":"Termedia Sp. z.o.o.","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Reumatologia"],"published-print":{"date-parts":[[2022,7,6]]},"abstract":"<jats:sec><jats:title>Introduction<\/jats:title><jats:p>The\u00a0results of\u00a0experimental and clinical studies in recent years indicate that the\u00a0transplantation of\u00a0multipotent mesenchymal stromal cells (MMSCs) is a\u00a0possible approach for the\u00a0\u201crestoration\u201d of\u00a0the\u00a0immune system of\u00a0patients with autoimmune diseases, in particular, rheumatoid arthritis. However, the\u00a0strength and duration of\u00a0the\u00a0effect vary greatly, which indicates incomplete correction of\u00a0the\u00a0tested parameters, thereby opening up the\u00a0prospect of\u00a0improving this method of\u00a0treatment by choosing dose-time parameters and methods of\u00a0their administration. The\u00a0aim of\u00a0this research was to determine the\u00a0indices of\u00a0cellular immunity in animals with adjuvant arthritis and therapy with cryopreserved MMSCs derived from adipose and cartilage tissues.<\/jats:p><\/jats:sec><jats:sec><jats:title>Material and methods<\/jats:title><jats:p>Adjuvant arthritis in male rats was modeled by subplantar administration of\u00a0Freund\u2019s complete adjuvant. On day 7 of\u00a0modeling, experimental animals were administered with saline (control group) or cryopreserved MMSCs from adipose or cartilaginous tissue locally or generalized. On day 28 after therapy the\u00a0body weight, spleen index and cellularity, and content of\u00a0CD3+, CD4+, CD8+, CD4+CD25+ cells in the\u00a0spleen were determined.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>In the\u00a0control group of\u00a0animals, the\u00a0inflammation was pronounced, as evidenced by a\u00a0significant increase in the\u00a0studied parameters throughout the\u00a0observation period. The\u00a0use of\u00a0cryopreserved MMSCs from adipose and cartilaginous tissues led to the\u00a0restoration of\u00a0T regulatory cells (Treg) on day 28. Generalized administration of\u00a0cells had a\u00a0more pronounced therapeutic effect compared to the\u00a0animals with local administration. These data can be used to justify and develop a\u00a0therapeutic approach to rheumatoid arthritis in clinical practice.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Cell therapy with cryopreserved MMSCs from investigated sources provided by both local and generalized administration to animals with adjuvant arthritis has a\u00a0correcting effect on the\u00a0cellular immunity.<\/jats:p><\/jats:sec>","DOI":"10.5114\/reum.2022.117842","type":"journal-article","created":{"date-parts":[[2022,7,13]],"date-time":"2022-07-13T11:52:31Z","timestamp":1657713151000},"page":"213-219","source":"Crossref","is-referenced-by-count":0,"title":["Role of cryopreserved multipotent mesenchymal stromal cells in modulation of some indices of cell immunity in adjuvant arthritis"],"prefix":"10.5114","volume":"60","author":[{"given":"Dmytro","family":"Vvedenskyi","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nataliia","family":"Volkova","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Natalya","family":"Babenko","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yulia","family":"Gaevska","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mariia","family":"Yukhta","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anatoliy","family":"Goltsev","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"3061","published-online":{"date-parts":[[2022,7,13]]},"reference":[{"key":"ref-786559","doi-asserted-by":"publisher","DOI":"10.1016\/j.intimp.2018.10.016"},{"key":"ref-786560","doi-asserted-by":"publisher","DOI":"10.3390\/ijms21197015"},{"key":"ref-786561","doi-asserted-by":"publisher","DOI":"10.1038\/s41584-018-0109-2"},{"key":"ref-786562","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2018.01.007"},{"key":"ref-786563","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbspin.2008.08.002"},{"key":"ref-786564","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbspin.2010.08.017"},{"key":"ref-786565","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(10)60826-4"},{"key":"ref-786566","doi-asserted-by":"publisher","DOI":"10.1038\/cddis.2015.327"},{"key":"ref-786567","doi-asserted-by":"publisher","DOI":"10.3390\/cells9040880"},{"key":"ref-786568","doi-asserted-by":"publisher","DOI":"10.1007\/s11892-020-01316-w"},{"key":"ref-786569","doi-asserted-by":"publisher","DOI":"10.1002\/art.30474"},{"key":"ref-786570","doi-asserted-by":"publisher","DOI":"10.22494\/cot.v4i2.64"},{"key":"ref-786571","first-page":"25","article-title":"Cryopreservation effect on proliferation and differentiation potential of cultured chorion cells","volume":"36","author":"Volkova NA","year":"2015","unstructured":"Volkova NA, Goltsev AN. Cryopreservation effect on proliferation and differentiation potential of cultured chorion cells. 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