{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T23:34:42Z","timestamp":1775086482316,"version":"3.50.1"},"reference-count":24,"publisher":"King Faisal Specialist Hospital and Research Centre","issue":"1","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Ann Saudi Med"],"published-print":{"date-parts":[[2024,1]]},"abstract":"<jats:sec><jats:title>BACKGROUND:<\/jats:title><jats:p>West Nile virus (WNV)-related illness is a global health problem. Understanding the seropositivity rates and identifying the risk factors related to WNV in various animal species including humans is crucial for the implementation of effective prevention strategies.<\/jats:p><\/jats:sec><jats:sec><jats:title>OBJECTIVES:<\/jats:title><jats:p>Assess the rate of seropositivity and the risk factors associated with WNV seropositivity.<\/jats:p><\/jats:sec><jats:sec><jats:title>DESIGN:<\/jats:title><jats:p>Descriptive, cross-sectional<\/jats:p><\/jats:sec><jats:sec><jats:title>SETTING:<\/jats:title><jats:p>Microbiology and virology departments in a veterinary college<\/jats:p><\/jats:sec><jats:sec><jats:title>PATIENTS AND METHODS:<\/jats:title><jats:p>In a sample of healthy human participants in Alanya, located close to regions where WNV activity has been detected, anti-WNV IgG antibody detection was performed using enzyme-linked immunosorbent assays. The positive results were confirmed by virus neutralization tests (VNTs). The sample was compared with a second group of age- and gender-matched healthy subjects selected from a previous cross-sectional study.<\/jats:p><\/jats:sec><jats:sec><jats:title>MAIN OUTCOME MEASURES:<\/jats:title><jats:p>Determination of the seropositivity and risk factors that were associated with WNV in healthy humans.<\/jats:p><\/jats:sec><jats:sec><jats:title>SAMPLE SIZE:<\/jats:title><jats:p>87 in current study; 356 in previous study.<\/jats:p><\/jats:sec><jats:sec><jats:title>RESULTS:<\/jats:title><jats:p>The first group of 87, which had a high risk of encountering vector mosquitoes, had a positivity rate of 8% (7\/87), whereas positivity in the second group was 4.5% (16\/356;<jats:italic>P<\/jats:italic>=.181). In the entire sample, the anti-WNV IgG antibody was positive in 23 out of 443 (5.2%) samples by the ELISA test. Among these 23 samples, ten were confirmed as positive using VNTs. Therefore, the WNV IgG seropositivity was 2.3% (10\/442). Confirmed IgG seropositivity rates were higher among male (3.8%) than female participants (0.9%;<jats:italic>P<\/jats:italic>=.054) and among adults aged \u226545 years (4%) than those aged 18-44 years (0.8%;<jats:italic>P<\/jats:italic>=.048).<\/jats:p><\/jats:sec><jats:sec><jats:title>CONCLUSION:<\/jats:title><jats:p>This study highlights the presence of WNV infection in the research region. More comprehensive and multidisciplinary studies are required to increase our knowledge about this zoonotic infection including risk factors in line with the One Health approach.<\/jats:p><\/jats:sec><jats:sec><jats:title>LIMITATIONS:<\/jats:title><jats:p>Small sample size.<\/jats:p><\/jats:sec>","DOI":"10.5144\/0256-4947.2024.48","type":"journal-article","created":{"date-parts":[[2024,2,5]],"date-time":"2024-02-05T06:41:44Z","timestamp":1707115304000},"page":"48-54","source":"Crossref","is-referenced-by-count":4,"title":["West Nile virus seropositivity in Alanya, a coastal city in the Mediterranean region of Turkey"],"prefix":"10.5144","volume":"44","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1225-7693","authenticated-orcid":true,"given":"Bayhan","family":"Bektore","sequence":"first","affiliation":[{"name":"From the Department of Medical Microbiology, Ministry of Health Alanya Alaadin Keykubat University, Alanya Education and Research Hospital, Antalya, Alanya, Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bora","family":"Dogan","sequence":"additional","affiliation":[{"name":"From the Department of Medical Microbiology, Urla State Hospital, Izmir, Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Akyut","family":"Ozkul","sequence":"additional","affiliation":[{"name":"From the Department of Virology, Faculty of Veterinary Medicine, Ankara University, Ankara, Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aysegul","family":"Gozalan","sequence":"additional","affiliation":[{"name":"From the Department of Medical Microbiology, Ministry of Health Alanya Alaadin Keykubat University, Alanya Education and Research Hospital, Antalya, Alanya, Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"3134","reference":[{"issue":"7","key":"e_1_3_3_2_2","doi-asserted-by":"crossref","first-page":"598","DOI":"10.3390\/pathogens9070589","article-title":"West Nile Virus: An Update on Pathobiology, Epidemiology, Diagnostics, Control and \u201cOne Health\u201d Implications","volume":"9","author":"Habarugira G","year":"2020","unstructured":"Habarugira G, Suen WW, Hobson-Peters J, Hall RA, Bielefeldt-Ohmann H. 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Arboviruses in the Mediterranean countries. Zbl. Bakt Suppl. 9. Stuttgart, Germany: Gustav Fischer Verlag; 1980. p. 155\u2013161."},{"issue":"4","key":"e_1_3_3_15_2","doi-asserted-by":"crossref","first-page":"826","DOI":"10.1017\/S0950268805005492","article-title":"Serological evidence of West Nile Virus (WNV) in mammalian species in Turkey","volume":"134","author":"Ozkul A","year":"2006","unstructured":"Ozkul A, Yildirim Y, Pinar D, Akcali A, Yilmaz V, Colak D. Serological evidence of West Nile Virus (WNV) in mammalian species in Turkey. Epidemiol Infect 2006;134(4):826\u20139.","journal-title":"Epidemiol Infect"},{"issue":"1","key":"e_1_3_3_16_2","first-page":"113","article-title":"Investigation of West Nile virus seroprevalence in Hacettepe University Hospital blood donors and confirmation of the positive results by plaque reduction neutralization test","volume":"45","author":"Ayturan S","year":"2011","unstructured":"Ayturan S, Aydo\u011fan S, Erg\u00fcnay K, Ozcebe OI, Us D. 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Clin Vaccine Immunol 2014;21(10):1460\u20132.","journal-title":"Clin Vaccine Immunol"},{"issue":"11","key":"e_1_3_3_19_2","first-page":"e31971","article-title":"West Nile Neuroinvasive Disease Treated With High-Dose Corticosteroids","volume":"14","author":"Kal S","year":"2022","unstructured":"Kal S, Beland A, Hasan M. West Nile Neuroinvasive Disease Treated With High-Dose Corticosteroids. Cureus 2022;14(11):e31971.","journal-title":"Cureus"},{"issue":"3","key":"e_1_3_3_20_2","doi-asserted-by":"crossref","first-page":"441","DOI":"10.1093\/cid\/ciaa749","article-title":"West Nile Virus-Associated Hospitalizations, California, 2004-2017","volume":"73","author":"Snyder RE","year":"2021","unstructured":"Snyder RE, Cooksey GS, Kramer V, Jain S, Vugia DJ. West Nile Virus-Associated Hospitalizations, California, 2004-2017. Clin Infect Dis 2021;73(3):441\u20137.","journal-title":"Clin Infect Dis"},{"issue":"6","key":"e_1_3_3_21_2","doi-asserted-by":"crossref","first-page":"2348","DOI":"10.1002\/vms3.589","article-title":"Sentinel serosurveillance of backyard hens proved West Nile virus circulation in the western provinces of Turkey","volume":"7","author":"Albayrak H","year":"2021","unstructured":"Albayrak H, Sahindokuyucu I, Muftuoglu B, Tamer C, Kadi H, Ozan E, et al. Sentinel serosurveillance of backyard hens proved West Nile virus circulation in the western provinces of Turkey. Vet Med Sci 2021;7(6):2348\u201352.","journal-title":"Vet Med Sci"},{"issue":"2","key":"e_1_3_3_22_2","doi-asserted-by":"crossref","first-page":"87","DOI":"10.5152\/kd.2015.16","article-title":"West Nile Virus Encephalitis in Alanya, Turkey: A Case Report","volume":"28","author":"Erdo\u011fan H","year":"2015","unstructured":"Erdo\u011fan H. West Nile Virus Encephalitis in Alanya, Turkey: A Case Report. Klimik Journal 2015;28(2):87\u20138.","journal-title":"Klimik Journal"},{"issue":"1","key":"e_1_3_3_23_2","first-page":"3","article-title":"West Nile arbovirus antibodies with hemagglutination inhibition (HI) in residents of Southeast Anatolia","volume":"11","author":"Meco O","year":"1977","unstructured":"Meco O. West Nile arbovirus antibodies with hemagglutination inhibition (HI) in residents of Southeast Anatolia. Mikrobiyol Bul. 1977;11(1):3\u201317.","journal-title":"Mikrobiyol Bul"},{"issue":"10","key":"e_1_3_3_24_2","doi-asserted-by":"crossref","first-page":"1094","DOI":"10.1111\/vox.13112","article-title":"West Nile virus and blood transfusion safety: A European perspective","volume":"116","author":"Garzon Jimenez RC","year":"2021","unstructured":"Garzon Jimenez RC, Lieshout-Krikke RW, Janssen MP. West Nile virus and blood transfusion safety: A European perspective. 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