{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,30]],"date-time":"2025-12-30T00:12:07Z","timestamp":1767053527366,"version":"3.48.0"},"reference-count":0,"publisher":"AVES YAYINCILIK A.\u015e.","issue":"1","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["EAJM"],"abstract":"<jats:p>Abstract\n\t\u00a0\n\n\tObjective: To evaluate the performance of the culture colorimetric detection assay MYCO WELL D-ONE\u00ae (MWD-ONE), designed to detect sexually transmitted infections using real-time polymerase chain reaction (PCR) as a reference method.\n\n\t\u00a0\n\n\tMaterials and Methods: One hundred and ten urogenital samples were screened for Gardnerella vaginalis (GV), Trichomonas vaginalis (TV), Mycoplasma hominis (MH), Mycoplasma spp., and Ureaplasma urealyticum (UU)\/Ureaplasma parvum (UP) using the MWD-ONE and real-time PCR assays Gardnerella vaginalis\/Lactobacillus species Real-TM Quant and Anyplex II STI-7 Detection, respectively.\n\n\t\u00a0\n\n\tResults: GV was detected in 33 samples by both the MWD-ONE and real-time PCR, while 6 samples gave discordant results. TV was detected by both MWD-ONE and Anyplex II STI-7 Detection kits in 3 samples, while 107 were negative. MH was detected by both methods in 5 cases, 4 samples gave discordant results, and 101 were negative. Mycoplasma genitalium (MG) was detected by Anyplex II STI-7 in 2 cases, 1 of which was detected as Mycoplasma spp. by MWD-ONE. Ten samples were positive by MWD-ONE, and 98 were negative with both assays. With regard to UU\/UP, 24 cases were detected by MWD-ONE and Anyplex PCR, 25 by PCR only, 4 by MWD-ONE, and 57 tested negative with both methods.\n\n\tThe positive predictive values (PPV) and negative predictive values (NPV) of the MWD-ONE assay for the pathogens tested were as following: GV, PPV 94.3%, NPV 94.7%; TV, PPV and NPV 100%; MH, PPV 71.4%, NPV 98.1%; Mycoplasma spp., PPV 9.1%, NPV 98.9%; and Ureaplasma spp., PPV 85.7 %, NPV 69.5 %. The agreement between the MWD-ONE and PCR was strong for GV and MH (k=0.8 and 0.7, respectively); perfect for TV (k=1); and moderate for UU\/UP (k=0.4).\n\n\t\u00a0\n\n\tConclusion: MWD-ONE assay appears to be suitable for routine testing of sexually transmitted infections.\n\n\t\u00a0\n\n\tCite this article as: Favalli C, Favaro M, Santi F, Piperno M, D\u2019Agostini C, Ciotti M. Performance Evaluation of a New Culture Colorimetric Detection Assay. Eurasian J Med 2019; 51(1): 5-7.<\/jats:p>","DOI":"10.5152\/eurasianjmed.2018.17364","type":"journal-article","created":{"date-parts":[[2018,10,9]],"date-time":"2018-10-09T08:32:57Z","timestamp":1539073977000},"page":"5-7","source":"Crossref","is-referenced-by-count":1,"title":["Performance Evaluation of a New Culture Colorimetric Detection Assay"],"prefix":"10.5152","volume":"51","author":[{"given":"Marco","family":"Favaro","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Flavia","family":"Santi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Micol","family":"Piperno","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marco","family":"Ciotti","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cartesio","family":"Favalli","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cartesio","family":"D' Agostini","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"3111","published-online":{"date-parts":[[2019,9,3]]},"container-title":["The Eurasian Journal of Medicine"],"original-title":[],"link":[{"URL":"https:\/\/eajm.org\/index.php\/pub\/article\/download\/2805\/2802","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,12,30]],"date-time":"2025-12-30T00:07:50Z","timestamp":1767053270000},"score":1,"resource":{"primary":{"URL":"https:\/\/eajm.org\/index.php\/pub\/article\/view\/2805"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,9,3]]},"references-count":0,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2019,1,1]]}},"URL":"https:\/\/doi.org\/10.5152\/eurasianjmed.2018.17364","relation":{},"ISSN":["1308-8742"],"issn-type":[{"type":"electronic","value":"1308-8742"}],"subject":[],"published":{"date-parts":[[2019,9,3]]}}}