{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,13]],"date-time":"2026-02-13T17:18:42Z","timestamp":1771003122578,"version":"3.50.1"},"reference-count":0,"publisher":"AVES YAYINCILIK A.\u015e.","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["EJR"],"abstract":"<jats:p>Abstract\n\tObjective: The presence of antinuclear antibodies (ANA), directed against intracellular antigens, is a distinctive feature of systemic autoimmune rheumatic diseases (SARDs). The standard test for antinuclear antibody screening is the indirect immunofluorescence (IIF). Anti-dense fine speckled 70 (anti-DFS70) antibodies were initially identified as an ANA IIF pattern from a patient with interstitial cystitis, but they were later associated with various other conditions. The objective of the study was to determine the frequency of anti-DFS70 antibodies in a cohort of patients undergoing routine ANA testing. \n\n\t\u00a0\n\n\tMaterial and Methods: From January 2011 to January 2012, a total of 5800 serum samples were screened for ANA by IIF (Euroimmune AG, L\u00fcbeck, Germany). DFS pattern was searched. \n\n\t\u00a0\n\n\tResults: ANA were present in 1302 (22.4%) of all patients. There were 16 (1.2%) anti-DFS70 antibody-positive patients. The number of females and males who have anti-DFS70 antibody was eleven and five, respectively. All of the samples presented a titer of \u22651\/320. There was one patient with SARD from the rheumatology department. Another 15 patients were from gastroenterology, endocrinology, and general internal medicine. \n\n\t\u00a0\n\n\tConclusion: Although a distinctive clinical association has not been reported, anti-DFS70 have been proposed as a significant biomarker for the exclusion of SARD. The present study is a preliminary study. There is a need for a reliable assay to ensure reactivity to DFS70 and screening large populations.\n<\/jats:p>","DOI":"10.5152\/eurjrheum.2015.0003","type":"journal-article","created":{"date-parts":[[2015,5,20]],"date-time":"2015-05-20T10:38:27Z","timestamp":1432118307000},"page":"103-105","source":"Crossref","is-referenced-by-count":8,"title":["Frequency of dense fine speckled pattern in immunofluorescence screening test"],"prefix":"10.5152","volume":"2","author":[{"given":"Asl\u0131 Gamze","family":"\u015eener","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"\u0130lhan","family":"Af\u015far","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"3111","published-online":{"date-parts":[[2019,9,3]]},"container-title":["European Journal of Rheumatology"],"original-title":[],"link":[{"URL":"https:\/\/www.eurjrheumatol.org\/index.php\/pub\/article\/download\/83\/83","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,7,29]],"date-time":"2025-07-29T07:29:11Z","timestamp":1753774151000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.eurjrheumatol.org\/index.php\/pub\/article\/view\/83"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,9,3]]},"references-count":0,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2019,9,3]]}},"URL":"https:\/\/doi.org\/10.5152\/eurjrheum.2015.0003","relation":{},"ISSN":["2148-4279"],"issn-type":[{"value":"2148-4279","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,9,3]]}}}