{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T07:34:57Z","timestamp":1785569697270,"version":"3.56.0"},"reference-count":0,"publisher":"Caleo","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Int Adv Otol."],"abstract":"<jats:p>Abstract\n\t\u00a0\n\n\tOBJECTIVES: Tricellulin is a tight junction (TJ)-forming protein that participates in the sealing function of tricellular TJs. Tricellulin-knockout (Tric\u2212\/\u2212) mice show progressive hearing loss with degeneration of hair cells in the cochlea without physiological or physical disorders. In the present study, we investigated the tricellulin expression and its deletion effects in the endolymphatic sac (ES) using Tric\u2212\/\u2212 mice.\n\n\t\u00a0\n\n\tMATERIALS and METHODS: The ES epithelia from wild-type (WT) mice were laser-microdissected, and RT-PCR was performed. The ES sections from Tric\u2212\/\u2212 and WT mice were immunostained with an anti-tricellulin antibody. Hematoxylin and eosin staining was performed for morphological examination. The inner ear of Tric\u2212\/\u2212 mice was perfused with biotinylation reagents, and the ES sections were observed for tracer permeability assay after applying streptavidin\u2013Alexa Fluor 488 conjugate.\n\n\t\u00a0\n\n\tRESULTS: The tricellulin expression was confirmed by RT-PCR and by immunohistochemistry in the WT ES. The ES in Tric\u2212\/\u2212 mice showed normal morphology and revealed no biotin leakage from the lumen.\n\n\t\u00a0\n\n\tCONCLUSION: The ES in Tric\u2212\/\u2212 mice showed no changes in morphology or disruption in macromolecular barrier function. The effects of solute leakages in the ES of Tric\u2212\/\u2212 mice may be very limited and compensatable, or that the ES epithelia may have other sealing system covering the lack of tricellulin.\n\n\t\u00a0\n\n\tCite this article as: Matsubara A, Miyashita T, Inamoto R, Sakaguchi H, Kamitani T, Mori N, et al. Tricellulin Expression and its Deletion Effects in the Endolymphatic Sac. J Int Adv Otol 2018; 14(2): 312-6.<\/jats:p>","DOI":"10.5152\/iao.2018.5473","type":"journal-article","created":{"date-parts":[[2018,8,9]],"date-time":"2018-08-09T05:13:43Z","timestamp":1533791623000},"page":"312-316","source":"Crossref","is-referenced-by-count":2,"title":["Tricellulin Expression and its Deletion Effects in the Endolymphatic Sac"],"prefix":"10.65717","volume":"14","author":[{"given":"Ai","family":"Matsubara","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takenori","family":"Miyashita","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ryuhei","family":"Inamoto","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hirofumi","family":"Sakaguchi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Toru","family":"Kamitani","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nozomu","family":"Mori","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroshi","family":"Hoshikawa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"55248","published-online":{"date-parts":[[2019,9,3]]},"container-title":["The Journal of International Advanced Otology"],"original-title":[],"link":[{"URL":"https:\/\/www.advancedotology.org\/index.php\/pub\/article\/download\/1040\/1038","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.advancedotology.org\/index.php\/pub\/article\/download\/998\/996","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.advancedotology.org\/index.php\/pub\/article\/download\/998\/996","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,1,22]],"date-time":"2026-01-22T11:33:55Z","timestamp":1769081635000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.advancedotology.org\/index.php\/pub\/article\/view\/998"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,9,3]]},"references-count":0,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2019,9,3]]}},"URL":"https:\/\/doi.org\/10.5152\/iao.2018.5473","relation":{},"ISSN":["2148-3817"],"issn-type":[{"value":"2148-3817","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,9,3]]}}}