{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,18]],"date-time":"2025-11-18T09:24:11Z","timestamp":1763457851796,"version":"3.45.0"},"reference-count":0,"publisher":"AVES YAYINCILIK A.\u015e.","issue":"2","license":[{"start":{"date-parts":[[2025,4,29]],"date-time":"2025-04-29T00:00:00Z","timestamp":1745884800000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["AOTT"],"abstract":"<jats:p>Objective: This study aimed to investigate the synaptic plasticity of the spinal dorsal horn regulated by the Piezo2\/P2X7\/MG signaling pathway and elucidate its role in the analgesic e!ects of massage in Cervical Spondylotic Radiculopathy (CSR), with the goal of identifying potential therapeutic targets for e!ective treatment.\r\nMethods: This experimental animal study included 40 Sprague-Dawley rats (180 \u00b1 20 g), randomly divided into four groups (control,model, sham-operated, and massage; n=10 per group) after a one-week acclimation period. The CSR model was established by spinal canal insertion. Blood and spinal cord tissues were collected post-intervention. Mechanical pain threshold (MPT), 50% paw withdrawal threshold (50% PWT), immunofluorescence, Western blot, and qRT-PCR assays were employed to evaluate synaptic plasticity and pathway expression.\r\nResults: The massage group demonstrated significantly higher MPT and 50% PWT values compared to the model group post-intervention (P &lt; 0.05). Downregulation of the Piezo2\/P2X7\/MG signaling pathway was observed in the massage group, correlating with a reduction in CSR progression, while upregulation in the model group promoted CSR (P &lt; 0.05).\r\nConclusion: The Piezo2\/P2X7\/MG signaling pathway regulates the synaptic plasticity of the spinal dorsal horn in CSR. These findingssuggest that massage therapy can e!ectively alleviate CSR-associated pain by modulating this pathway, o!ering a potential noninvasive\u00a0\r\nCite this article as: Zhou K, Yang S, Duan X, et al. Role of Piezo2\/P2X7\/MG pathway in synaptic plasticity and analgesic e\"ects of massage therapy in cervicalnspondylotic radiculopathy: A preclinical study in animal models. Acta Orthop Traumatol Turc., 2025;59(2):111-116.<\/jats:p>","DOI":"10.5152\/j.aott.2024.24097","type":"journal-article","created":{"date-parts":[[2025,5,7]],"date-time":"2025-05-07T09:09:57Z","timestamp":1746608997000},"page":"111-116","source":"Crossref","is-referenced-by-count":0,"title":["Role of Piezo2\/P2X7\/MG pathway in synaptic plasticity and analgesic effects of massage therapy in cervical spondylotic radiculopathy: A preclinical study in animal models"],"prefix":"10.5152","volume":"59","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5303-7937","authenticated-orcid":false,"given":"Xueyu","family":"Jiang","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-1027-8009","authenticated-orcid":false,"given":"Song","family":"Yang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-5828-3836","authenticated-orcid":false,"given":"Xiaxia","family":"Duan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-7400-3359","authenticated-orcid":false,"given":"Keying","family":"Liao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-1129-4358","authenticated-orcid":false,"given":"Siqi","family":"Li","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-0091-6029","authenticated-orcid":false,"given":"Zhifang","family":"Qiu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-9311-388X","authenticated-orcid":false,"given":"Jiaxuan","family":"Tan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-3657-297X","authenticated-orcid":false,"given":"Lingyao","family":"Xie","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-6955-7260","authenticated-orcid":false,"given":"Yaoyao","family":"Xiao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-7938-7371","authenticated-orcid":false,"given":"Jiani","family":"Yang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-1843-4179","authenticated-orcid":false,"given":"Kuiming","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"3111","published-online":{"date-parts":[[2025,4,29]]},"container-title":["Acta Orthopaedica et Traumatologica Turcica"],"original-title":[],"link":[{"URL":"https:\/\/aott.org.tr\/index.php\/pub\/article\/download\/3984\/3925","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/aott.org.tr\/index.php\/pub\/article\/download\/3984\/3925","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,11,18]],"date-time":"2025-11-18T09:12:06Z","timestamp":1763457126000},"score":1,"resource":{"primary":{"URL":"https:\/\/aott.org.tr\/index.php\/pub\/article\/view\/3984"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,4,29]]},"references-count":0,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2025,4,30]]}},"URL":"https:\/\/doi.org\/10.5152\/j.aott.2024.24097","relation":{},"ISSN":["2589-1294","1017-995X"],"issn-type":[{"type":"electronic","value":"2589-1294"},{"type":"print","value":"1017-995X"}],"subject":[],"published":{"date-parts":[[2025,4,29]]}}}