{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,8]],"date-time":"2026-07-08T10:31:53Z","timestamp":1783506713782,"version":"3.55.0"},"reference-count":0,"publisher":"AVES YAYINCILIK A.\u015e.","issue":"1","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Turkish Journal of Gastroenterology"],"abstract":"<jats:p>Background: The precise pathogenesis of irritable bowel syndrome (IBS) remains unresolved; however, recent studies have reported that\n\npatients with diarrhea-predominant IBS exhibit an increased small intestinal permeability and increased number of enterochromaffin\n\ncells containing high 5-hydroxytryptamine (5HT; serotonin) levels. In this study, we investigated whether 5HT has the potential to modulate small intestinal epithelial cell permeability, focusing on tight junction-associated proteins.\n\nMethods: The differentiated Caco-2 cell monolayer on porous filters (Millicell) was used. Then, 5HT was added to the lower Millicell compartment for 7 days. Intestinal epithelial cell permeability was assessed by measuring the flux of paracellular permeability markers. We\n\nfurther assessed the expression of occludin in the 5HT-stimulated Caco-2 monolayer.\n\nResults: We found that 5HT did not affect the viability of Caco-2 cells at concentrations up to 100 \u00b5M during the experimental period.\n\nAdministration of 5HT to the basal side of Caco-2 cells increased the flux of 3H-labeled mannitol (182 Da) but did not increase that of\n\nFITC-dextran (4000 Da). Among the tight junction proteins, the expression of occludin was specifically decreased by stimulation with\n\n5HT at a concentration of 100 \u00b5M.\n\nConclusion: In conclusion, excessive 5HT in the basal side increased the permeability of intestinal epithelial cells via reduction of occludin expression.<\/jats:p>","DOI":"10.5152\/tjg.2022.20691","type":"journal-article","created":{"date-parts":[[2022,1,12]],"date-time":"2022-01-12T14:06:09Z","timestamp":1641996369000},"page":"74-79","source":"Crossref","is-referenced-by-count":5,"title":["Subepithelial Serotonin Reduces Small Intestinal Epithelial Cell Tightness via Reduction of Occluding Expression"],"prefix":"10.5152","volume":"33","author":[{"given":"Hideki","family":"Horie","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Osamu","family":"Handa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuji","family":"Naito","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Atsushi","family":"Majima","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuriko","family":"Yasuda-Onozawa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yukiko","family":"Uehara","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kazuhiro","family":"Kamada","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kazuhiro","family":"Katada","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kazuhiko","family":"Uchiyama","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takeshi","family":"Ishikawa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tomohisa","family":"Takagi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoshito","family":"Itoh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Akiko","family":"Shiotani","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"3111","published-online":{"date-parts":[[2022,1,15]]},"container-title":["Turkish Journal of Gastroenterology"],"original-title":[],"link":[{"URL":"https:\/\/www.turkjgastroenterol.org\/index.php\/tjg\/article\/download\/3830\/3492","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,2,18]],"date-time":"2026-02-18T02:34:15Z","timestamp":1771382055000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.turkjgastroenterol.org\/index.php\/tjg\/article\/view\/3830"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,1,15]]},"references-count":0,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2022,1,15]]}},"URL":"https:\/\/doi.org\/10.5152\/tjg.2022.20691","relation":{},"ISSN":["2148-5607"],"issn-type":[{"value":"2148-5607","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,1,15]]}}}