{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,17]],"date-time":"2026-02-17T03:08:14Z","timestamp":1771297694657,"version":"3.50.1"},"reference-count":0,"publisher":"AVES YAYINCILIK A.\u015e.","issue":"6","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Turkish Journal of Gastroenterology"],"abstract":"<jats:p>Background\/Aims: The pathogenesis mechanism of acute gastric mucosal lesions (AGML) is still unclear; further exploration is urgently needed to find a new therapeutic target. This study aimed to investigate whether morphine might regulate the expression and function of transient receptor potential ankyrin 1 (TRPA1) through a cyclic adenosine monophosphate\/protein kinase A (cAMP\/PKA)-dependent pathway, thereby alleviating gastric mucosal lesions caused by water-immersion restraint stress (WIRS). Materials and Methods: Rats were administered with intrathecal morphine, TRPA1 antagonist (HC-030031), &amp;micro;-opioid receptor antagonist, or protein kinase A inhibitor (H-89), respectively, before WIRS. After 6 hours of WIRS, microscopic lesions, hematoxylin and eosin staining, and transmission electron microscopy were applied to assess the damage of the gastric mucosa. Real-time polymerase chain reaction, Western blot, and enzyme-linked immunosorbent assay were conducted to detect the levels of TRPA1 and substance P (SP) in the dorsal root ganglia (DRG) and gastric tissues. In addition, immunofluorescence was used to explore the possible co-expression of TRPA1 and &amp;micro;-opioid receptors in the DRG. Results: The results indicated that WIRS upregulated TRPA1 and SP in gastric mucosa, and HC-030031 or H-89 could alleviate gastric mucosal lesions caused by WIRS (P &amp;lt; .0001). Morphine was found to suppress both WIRS-induced gastric mucosal lesions (P &amp;lt; .0001) and the upregulation of TRPA1 (P = .0086) and SP (P = .0013). Conclusion: Both TRPA1 and SP play important roles in the pathogenesis of WIRS-induced AGML. Exogenous gastroprotective strategies reduce elevated levels of TRPA1 via the cAMP\/PKA-dependent pathway. Inhibition of TRPA1 upregulation in the DRG is critical for intrathecal morphine preconditioning-induced gastric protection. Cite this article as: Jiang Q, Jiang P, Guo M, et al. Inhibition of dorsal root ganglia transient receptor protein ankyrin 1 upregulation contributes to the protective effect of morphine against gastric mucosal damage induced by water-immersion restraint stress. Turk J Gastroenterol. 2024;35(6):453-464.<\/jats:p>","DOI":"10.5152\/tjg.2024.23267","type":"journal-article","created":{"date-parts":[[2024,2,26]],"date-time":"2024-02-26T06:03:42Z","timestamp":1708927422000},"page":"453-464","source":"Crossref","is-referenced-by-count":0,"title":["Inhibition of Dorsal Root Ganglia Transient Receptor Potential Ankyrin 1 Upregulation Contributes to the Protective Effect of Morphine Against Gastric Mucosal Damage Induced by Water-Immersion Restraint Stress"],"prefix":"10.5152","volume":"35","author":[{"given":"Qun","family":"Jiang","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peng","family":"Jiang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mingyan","family":"Guo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chuangbo","family":"Xie","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qiong","family":"Ling","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gaofeng","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Weifeng","family":"Tu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiangyu","family":"Li","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"3111","published-online":{"date-parts":[[2024,6,15]]},"container-title":["Turkish Journal of Gastroenterology"],"original-title":[],"link":[{"URL":"http:\/\/turkjgastroenterol.org\/index.php\/tjg\/article\/download\/4118\/3780","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,2,17]],"date-time":"2026-02-17T02:37:15Z","timestamp":1771295835000},"score":1,"resource":{"primary":{"URL":"http:\/\/turkjgastroenterol.org\/index.php\/tjg\/article\/view\/4118"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,6,15]]},"references-count":0,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2024,6,15]]}},"URL":"https:\/\/doi.org\/10.5152\/tjg.2024.23267","relation":{},"ISSN":["2148-5607"],"issn-type":[{"value":"2148-5607","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,6,15]]}}}