{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,24]],"date-time":"2025-12-24T13:59:19Z","timestamp":1766584759589,"version":"3.48.0"},"reference-count":0,"publisher":"AVES YAYINCILIK A.\u015e.","license":[{"start":{"date-parts":[[2025,11,21]],"date-time":"2025-11-21T00:00:00Z","timestamp":1763683200000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Turkish Journal of Gastroenterology"],"abstract":"<jats:p>Background\/Aims: miR-3180-3p has been linked to hepatocellular carcinoma progression, but its role and molecular mechanisms in metabolic dysfunction-associated steatotic liver disease (MASLD) are still unclear. The objective is to explore whether miR-3180-3p serves as a diagnostic biomarker for MASLD and elucidate its function in mediating hepatic inflammatory and oxidative stress responses.\nMaterials and Methods: This cross-sectional study included 117 patients with MASLD and 100 healthy controls. Serum and cellular RNA were extracted for real-time polymerase chain reaction analysis. In vitro experiments utilized HepG2 cells treated with free fatty acids (FFAs) to mimic MASLD-related stress conditions, evaluating miR-3180-3p expression levels and its effects on oxidative stress mark ers (malondialdehyde, superoxide dismutase) and inflammatory cytokines (tumor necrosis factor-\u03b1, interleukin-6 (IL-6), IL-1\u03b2). Target genes of miR-3180-3p were identified using bioinformatics and dual-luciferase assays.\nResults: Serum miR-3180-3p levels were significantly higher in MASLD patients than controls, showing strong diagnostic value and potential as a biomarker. Multivariate analysis identified it, along with C-reactive protein (CRP) and high-density lipoprotein (HDL) cholesterol, as an independent risk factor for MASLD. Its expression positively correlated with triglycerides, low-density lipoprotein cholesterol, white blood cell count, CRP, Fibrosis-4, and oxidative stress markers (OA, PA), but negatively with HDL cholesterol. In FFA-treated cells, higher miR-3180-3p was linked to increased oxidative stress and inflammation. SKI, a direct target of miR-3180-3p, when silenced, reversed the protective effects of miR-3180-3p inhibition against FFA-induced cellular damage.\nConclusion: miR-3180-3p is a promising diagnostic biomarker for MASLD and contributes to disease progression by enhancing hepatic inflammation and oxidative stress via targeting SKI.\n\u00a0\nCite this article as: Song K, Qi P. The diagnostic potential and regulatory mechanisms of miR-3180-3p in metabolic dysfunction associated fatty liver disease with respect to inflammation and oxidative stress. Turk J Gastroenterol. Published online November 21, 2025. doi:10.5152\/tjg.2025.25346.<\/jats:p>","DOI":"10.5152\/tjg.2025.25346","type":"journal-article","created":{"date-parts":[[2025,11,21]],"date-time":"2025-11-21T13:28:47Z","timestamp":1763731727000},"source":"Crossref","is-referenced-by-count":0,"title":["The Diagnostic Potential and Regulatory Mechanisms  of miR-3180-3p in Metabolic Dysfunction\u2013Associated  Fatty Liver Disease with Respect to Inflammation and  Oxidative Stress"],"prefix":"10.5152","author":[{"ORCID":"https:\/\/orcid.org\/0009-0001-9966-7395","authenticated-orcid":false,"given":"Kexiu","family":"Song","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-3528-9479","authenticated-orcid":false,"given":"Peng","family":"Qi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"3111","published-online":{"date-parts":[[2025,11,21]]},"container-title":["Turkish Journal of Gastroenterology"],"original-title":[],"link":[{"URL":"https:\/\/www.turkjgastroenterol.org\/index.php\/tjg\/article\/download\/4324\/3989","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.turkjgastroenterol.org\/index.php\/tjg\/article\/download\/4324\/3989","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,12,24]],"date-time":"2025-12-24T13:56:17Z","timestamp":1766584577000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.turkjgastroenterol.org\/index.php\/tjg\/article\/view\/4324"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,11,21]]},"references-count":0,"URL":"https:\/\/doi.org\/10.5152\/tjg.2025.25346","relation":{},"ISSN":["2148-5607"],"issn-type":[{"type":"electronic","value":"2148-5607"}],"subject":[],"published":{"date-parts":[[2025,11,21]]}}}