{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,17]],"date-time":"2026-03-17T12:01:47Z","timestamp":1773748907101,"version":"3.50.1"},"reference-count":0,"publisher":"AVES YAYINCILIK A.\u015e.","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Turkish Journal of Gastroenterology"],"abstract":"<jats:p>Background\/Aims: This study aims to elucidate both the diagnostic potential of miR-487b-3p for insulin resistance and its regulatory mechanisms in patients with nonalcoholic fatty liver disease (NAFLD).Materials and Methods: The study group included 60 NAFLD patients and 60 healthy controls. The expression levels of miR-487b-3p and insulin receptor substrate 1 (IRS1) in serum and cells were quantified by RT-qPCR. The diagnostic utility of the microRNA was evaluated through receiver operating characteristic (ROC) analysis. Glucose uptake in HepG2 was measured using fluorescence-based assays. The interaction between miR-487b-3p and IRS1 was confirmed by dual-luciferase reporter assay.Results: Compared with controls, NAFLD patients exhibited significantly elevated serum miR-487b-3p levels (P &lt; .05). The ROC analysis demonstrated high diagnostic accuracy of miR-487b-3p in distinguishing between healthy controls and NAFLD patients. Functional analyses demonstrated that miR-487b-3p overexpression suppressed both glucose uptake and IRS1 expression (P &lt; .05), whereas miR487b-3p inhibition enhanced glucose uptake and IRS1 expression (P &lt; .05). Mechanistically, miR-487b-3p negatively regulated IRS1 to modulate glucose uptake.Conclusion: miR-487b-3p may serve as a potential diagnostic biomarker for NAFLD, and miR-487b-3p negatively regulates IRS1 to modulate glucose uptake, thereby impairing insulin sensitivity in NAFLD patients.Cite this article as: Chen M, Tan J, Huang M, et al. Deregulated miR-487b-3p in patients with non-alcoholic fatty liver disease and its regulatory effect on insulin resistance. Turk J Gastroenterol. 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