{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,18]],"date-time":"2026-06-18T13:39:40Z","timestamp":1781789980519,"version":"3.54.5"},"reference-count":31,"publisher":"Korean Society of Animal Science and Technology","issue":"1","license":[{"start":{"date-parts":[[2021,1,1]],"date-time":"2021-01-01T00:00:00Z","timestamp":1609459200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0"}],"funder":[{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea","doi-asserted-by":"publisher","award":["NRF-2013R1A6A3A04063769"],"award-info":[{"award-number":["NRF-2013R1A6A3A04063769"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.ejast.org"],"crossmark-restriction":true},"short-container-title":["J Anim Sci Technol"],"published-print":{"date-parts":[[2021,1]]},"DOI":"10.5187\/jast.2021.e8","type":"journal-article","created":{"date-parts":[[2020,12,23]],"date-time":"2020-12-23T10:00:34Z","timestamp":1608717634000},"page":"58-68","update-policy":"https:\/\/doi.org\/10.5187\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Artemisia vulgaris extract causes precocious acrosome reaction and viability loss but low rate of membrane damage in mouse spermatozoa"],"prefix":"10.5187","volume":"63","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6913-9268","authenticated-orcid":false,"given":"Sabina","family":"Bhandari","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5123-7396","authenticated-orcid":false,"given":"Jayaswori","family":"Sharma","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1520-7325","authenticated-orcid":false,"given":"Sarbesh","family":"Rizal","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7167-5123","authenticated-orcid":false,"given":"Young-Joo","family":"Yi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8145-9475","authenticated-orcid":false,"given":"Gaurishankar","family":"Manandhar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"3132","published-online":{"date-parts":[[2021,1,31]]},"reference":[{"key":"key2.0210215064923e+13_B1","doi-asserted-by":"crossref","first-page":"1083","DOI":"10.1001\/jama.2018.0448","article-title":"Weighing the risks and benefits of hormonal contraception","volume":"319","author":"Peachman","year":"2018","journal-title":"JAMA"},{"key":"key2.0210215064923e+13_B2","doi-asserted-by":"crossref","first-page":"382","DOI":"10.1016\/j.jcma.2015.03.008","article-title":"Antifertility activity of medicinal plants","volume":"78","author":"Daniyal","year":"2015","journal-title":"J Chin Med Assoc."},{"key":"key2.0210215064923e+13_B3","first-page":"587","article-title":"Reversible contraceptive efficacy and safety evaluation of ethanolic extract of Tinospora cordifolia in animal model","volume":"3","author":"Singh","year":"2011","journal-title":"Arch Appl Sci Res."},{"key":"key2.0210215064923e+13_B4","first-page":"83","article-title":"Antifertility effect of Iranian neem seed alcoholic extract on epididymal sperm of mice","volume":"3","author":"Dehghan","year":"2005","journal-title":"J Reprod BioMed."},{"key":"key2.0210215064923e+13_B5","doi-asserted-by":"crossref","first-page":"68","DOI":"10.3390\/medicines4030068","article-title":"The Genus Artemisia: a 2012\u20132017 literature review on chemical composition, antimicrobial, insecticidal and antioxidant activities of essential oils","volume":"4","author":"Pandey","year":"2017","journal-title":"Medicines"},{"key":"key2.0210215064923e+13_B6","doi-asserted-by":"crossref","first-page":"403","DOI":"10.1016\/j.foodres.2018.03.072","article-title":"Towards a better understanding of Artemisia vulgaris: botany, phytochemistry, pharmacological and biotechnological potential","volume":"109","author":"Abiri","year":"2018","journal-title":"Food Res Int."},{"key":"key2.0210215064923e+13_B7","doi-asserted-by":"crossref","first-page":"205","DOI":"10.1615\/JEnvironPatholToxicolOncol.2019029397","article-title":"Therapeutic potential of Artemisia vulgaris: an insight into underlying immunological mechanisms","volume":"38","author":"Soon","year":"2019","journal-title":"J Environ Pathol Toxicol Oncol."},{"key":"key2.0210215064923e+13_B8","doi-asserted-by":"crossref","first-page":"1175","DOI":"10.1007\/s11427-015-4948-7","article-title":"The discovery of artemisinin and the nobel prize in physiology or medicine","volume":"58","author":"Su","year":"2015","journal-title":"Sci China Life Sci."},{"key":"key2.0210215064923e+13_B9","doi-asserted-by":"crossref","first-page":"49","DOI":"10.3390\/ph12020049","article-title":"Chemical profile and biological activities of essential oil from Artemisia vulgaris L. cultivated in Brazil","volume":"12","author":"Malik","year":"2019","journal-title":"Pharmaceuticals"},{"key":"key2.0210215064923e+13_B10","first-page":"1651","article-title":"Bioactivities of volatile components from Nepalese artemisia species","volume":"7","author":"Satyal","year":"2012","journal-title":"Nat Prod Commun."},{"key":"key2.0210215064923e+13_B11","first-page":"180","article-title":"Antifertility activity of Artemisia vulgaris leaves on female wistar rats","volume":"12","author":"Shaik","year":"2014","journal-title":"Chin J Nat Med."},{"key":"key2.0210215064923e+13_B12","first-page":"101","article-title":"Effect of Artemisia vulgaris isolated extract and determination of AD50 and LD50 for antifertility and mortality of Callosobruchus chinensis (L.)","volume":"11","author":"Pal","year":"2011","journal-title":"J Ecophysiol Occup Hlth."},{"key":"key2.0210215064923e+13_B13","first-page":"10","article-title":"Experimental studies on Artemisia vulgaris: a possible antifertility drug","volume":"14","author":"Narwaria","year":"1994","journal-title":"Anc Sci Life"},{"key":"key2.0210215064923e+13_B14","doi-asserted-by":"crossref","first-page":"647","DOI":"10.1002\/ptr.4760","article-title":"A safety assessment of the antimalarial herb Artemisia annua during pregnancy in Wistar rats","volume":"27","author":"Abolaji","year":"2013","journal-title":"Phytother Res."},{"key":"key2.0210215064923e+13_B15","doi-asserted-by":"crossref","first-page":"445","DOI":"10.1002\/(SICI)1098-2795(199904)52:4<445::AID-MRD14>3.0.CO;2-6","article-title":"Simple histochemical stain for acrosomes on sperm from several species","volume":"52","author":"Larson","year":"1999","journal-title":"Mol Reprod Dev."},{"key":"key2.0210215064923e+13_B16","first-page":"A3.B.1","article-title":"Trypan blue exclusion test of cell viability","volume":"111","author":"Strober","year":"1997","journal-title":"Curr Protoc Immunol."},{"key":"key2.0210215064923e+13_B17","volume-title":"WHO laboratory manual for the examination and processing of human semen","author":"World Health Organization [WHO]","year":"2010","edition":"5th ed"},{"key":"key2.0210215064923e+13_B18","doi-asserted-by":"crossref","first-page":"219","DOI":"10.1530\/jrf.0.0700219","article-title":"Development of an assay to assess the functional integrity of the human sperm membrane and its relationship to other semen characteristics","volume":"70","author":"Jeyendran","year":"1984","journal-title":"Reproduction"},{"key":"key2.0210215064923e+13_B19","doi-asserted-by":"crossref","first-page":"116","DOI":"10.1002\/mrd.21276","article-title":"Can curcumin provide an ideal contraceptive?","volume":"78","author":"Naz","year":"2011","journal-title":"Mol Reprod Dev."},{"key":"key2.0210215064923e+13_B20","unstructured":"MEDCALC Free statistical calculators [Internet] 2020 Aug 8 https:\/\/www.medcalc.org\/calc\/comparison_of_means.php?fbclid=IwAR3UoPsut1u1k38yCOqwAp1mheuxrBIkaZnGY4Q24zv-cVCOgGohrY_kwQ"},{"key":"key2.0210215064923e+13_B21","doi-asserted-by":"crossref","first-page":"371","DOI":"10.1017\/S0967199400002240","article-title":"Fertility of mammalian spermatozoa: its development and relativity","volume":"2","author":"Yanagimachi","year":"1994","journal-title":"Zygote."},{"key":"key2.0210215064923e+13_B22","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1016\/S1001-7844(07)60003-0","article-title":"Evaluation on sperm acrosome integrity of infertile men with varicocele","volume":"18","author":"Tzvetkova","year":"2007","journal-title":"J Reprodd Contracep."},{"key":"key2.0210215064923e+13_B23","doi-asserted-by":"crossref","first-page":"148","DOI":"10.1016\/j.apjr.2016.01.012","article-title":"Effect of heparin, caffeine and calcium ionophore A23187 on in vitro induction of the acrosome reaction of fresh ram spermatozoa","volume":"5","author":"El-Shahat","year":"2016","journal-title":"Asian Pac J Reprod"},{"key":"key2.0210215064923e+13_B24","doi-asserted-by":"crossref","first-page":"1425","DOI":"10.1095\/biolreprod65.5.1425","article-title":"Exposure of sperm head equatorin after acrosome reaction and its fate after fertilization in mice","volume":"65","author":"Manandhar","year":"2001","journal-title":"Biol Reprod."},{"key":"key2.0210215064923e+13_B25","doi-asserted-by":"crossref","first-page":"18543","DOI":"10.1073\/pnas.1317113110","article-title":"Ca2+ ionophore A23187 can make mouse spermatozoa capable of fertilizing in vitro without activation of cAMP-dependent phosphorylation pathways","volume":"110","author":"Tateno","year":"2013","journal-title":"Proc Natl Acad Sci USA"},{"key":"key2.0210215064923e+13_B26","doi-asserted-by":"crossref","first-page":"1168","DOI":"10.1095\/biolreprod.108.071738","article-title":"Peroxiredoxin 2 and peroxidase enzymatic activity of mammalian spermatozoa","volume":"80","author":"Manandhar","year":"2009","journal-title":"Biol Reprod."},{"key":"key2.0210215064923e+13_B27","doi-asserted-by":"crossref","first-page":"674","DOI":"10.1002\/j.1939-4640.1996.tb01852.x","article-title":"Use of peanut agglutinin to access the acrosomal status and the zona pellucida-induced acrosome reaction in stallion spermatozoa","volume":"17","author":"Cheng","year":"1996","journal-title":"J Androl."},{"key":"key2.0210215064923e+13_B28","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1002\/mrd.1080260110","article-title":"Ca2+ uptake during capacitation of mouse spermatozoa and the effect of an anion transport inhibitor on Ca2+ uptake","volume":"26","author":"Ruknudin","year":"1990","journal-title":"Mol Reprod Dev."},{"key":"key2.0210215064923e+13_B29","doi-asserted-by":"crossref","first-page":"357","DOI":"10.1002\/j.1939-4640.1988.tb01065.x","article-title":"Ethanol accelerates acrosomal loss in human spermatozoa","volume":"9","author":"Alvarez","year":"1988","journal-title":"J Androl."},{"key":"key2.0210215064923e+13_B30","doi-asserted-by":"crossref","first-page":"369","DOI":"10.1111\/j.1439-0531.2009.01420.x","article-title":"Effect of catalase and superoxide dismutase on motility, viability and acrosomal integrity of frozen-thawed cat spermatozoa","volume":"44","author":"Thiangtum","year":"2009","journal-title":"Reprod Domest Anim."},{"key":"key2.0210215064923e+13_B31","doi-asserted-by":"crossref","first-page":"355","DOI":"10.1016\/j.contraception.2009.09.001","article-title":"Comparative study on the spermicidal activity of organic solvent fractions from hydroethanolic extracts of Achyranthes aspera and Stephania hernandifolia in human and rat sperm","volume":"81","author":"Paul","year":"2010","journal-title":"Contraception"}],"container-title":["Journal of Animal Science and Technology"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/www.ejast.org\/download\/download_pdf?doi=10.5187\/jast.2021.e8","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/www.ejast.org\/download\/download_pdf?doi=10.5187\/jast.2021.e8","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,28]],"date-time":"2023-10-28T12:34:05Z","timestamp":1698496445000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.ejast.org\/archive\/view_article?doi=10.5187\/jast.2021.e8"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,1]]},"references-count":31,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2021,1,31]]},"published-print":{"date-parts":[[2021,1]]}},"alternative-id":["10.5187\/jast.2021.e8"],"URL":"https:\/\/doi.org\/10.5187\/jast.2021.e8","relation":{},"ISSN":["2672-0191","2055-0391"],"issn-type":[{"value":"2672-0191","type":"print"},{"value":"2055-0391","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,1]]},"assertion":[{"value":"2020-08-17","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-10-28","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}}]}}