{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T02:54:44Z","timestamp":1790909684115,"version":"4.1.0"},"reference-count":53,"publisher":"Korean Society of Animal Science and Technology","issue":"3","license":[{"start":{"date-parts":[[2022,5,1]],"date-time":"2022-05-01T00:00:00Z","timestamp":1651363200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0"}],"funder":[{"name":"National Research Foundation of Korea","award":["2019R1l1A3A01060532"],"award-info":[{"award-number":["2019R1l1A3A01060532"]}],"id":[{"id":"https:\/\/ror.org\/013aysd81","id-type":"ROR","asserted-by":"crossref"}]},{"name":"Rural Development Administration","award":["PJ015583"],"award-info":[{"award-number":["PJ015583"]}],"id":[{"id":"https:\/\/ror.org\/03xs9yg50","id-type":"ROR","asserted-by":"crossref"}]}],"content-domain":{"domain":["www.ejast.org"],"crossmark-restriction":true},"short-container-title":["J Anim Sci Technol"],"published-print":{"date-parts":[[2022,5]]},"abstract":"<jats:p>The blood-epididymis barrier (BEB) forms a unique microenvironment that is\n                    crucial for the maturation, protection, transport, and storage of spermatozoa in\n                    the epididymis. To characterize the function of tight junctions (TJs), which are\n                    constitutive components of the BEB, we determined the expression and\n                    localization of TJ proteins such as zonula occludens (ZO)-1, 2, and 3, occludin,\n                    and claudin3 (Cldn3) during postnatal development in the goat epididymis. To\n                    assess the expression patterns of TJ proteins in immature (3 months of age) and\n                    mature (14 months of age) goat epididymides, two different experimental methods\n                    were used including immunofluorescence labeling and western blotting. We show\n                    that, ZO-1, 2, and 3, and occludin, were strictly expressed and localized to the\n                    TJs of the goat epididymis, whereas Cldn3 was present in basolateral membranes\n                    as well as TJs. All TJ proteins examined were more highly expressed in the\n                    immature epididymis compared to levels in mature tissue. In conclusion, our\n                    study indicates that at least five TJ proteins, namely ZO-1, ZO-2, ZO-3,\n                    occludin, and Cldn3, are present in TJs, and the expression strength and pattern\n                    of TJ proteins tend to be age dependent in the goat epididymis. Together, these\n                    data suggest that the distinct expression patterns of TJ proteins are essential\n                    for regulating components of the luminal contents in the epididymal epithelium\n                    and for forming adequate luminal conditions that are necessary for the\n                    maturation, protection, transport, and storage of spermatozoa in the goat\n                    epididymis.<\/jats:p>","DOI":"10.5187\/jast.2022.e13","type":"journal-article","created":{"date-parts":[[2022,3,27]],"date-time":"2022-03-27T21:51:53Z","timestamp":1648417913000},"page":"500-514","update-policy":"https:\/\/doi.org\/10.5187\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Differential expression and localization of tight junction proteins\n                    in the goat epididymis"],"prefix":"10.5187","volume":"64","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8521-3010","authenticated-orcid":false,"given":"Sung Woo","family":"Kim","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03xs9yg50","id-type":"ROR","asserted-by":"publisher"}],"name":"Animal Genetic Resource Research Center, National Institute of Animal Science, Rural Development Administration, Hamyang 50000, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0569-9004","authenticated-orcid":false,"given":"Yu-Da","family":"Jeong","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/0373nm262","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Animal Resources Science, Kongju National University, Yesan 32439, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8208-8728","authenticated-orcid":false,"given":"Ga-Yeong","family":"Lee","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/0373nm262","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Animal Resources Science, Kongju National University, Yesan 32439, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9019-1653","authenticated-orcid":false,"given":"Jinwook","family":"Lee","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03xs9yg50","id-type":"ROR","asserted-by":"publisher"}],"name":"Animal Genetic Resource Research Center, National Institute of Animal Science, Rural Development Administration, Hamyang 50000, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2965-7826","authenticated-orcid":false,"given":"Jae-Yeung","family":"Lee","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03xs9yg50","id-type":"ROR","asserted-by":"publisher"}],"name":"Animal Genetic Resource Research Center, National Institute of Animal Science, Rural Development Administration, Hamyang 50000, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2826-3171","authenticated-orcid":false,"given":"Chan-Lan","family":"Kim","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03xs9yg50","id-type":"ROR","asserted-by":"publisher"}],"name":"Animal Genetic Resource Research Center, National Institute of Animal Science, Rural Development Administration, Hamyang 50000, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6108-6325","authenticated-orcid":false,"given":"Yeoung-Gyu","family":"Ko","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03xs9yg50","id-type":"ROR","asserted-by":"publisher"}],"name":"Animal Genetic Resource Research Center, National Institute of Animal Science, Rural Development Administration, Hamyang 50000, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1255-9509","authenticated-orcid":false,"given":"Sung-Soo","family":"Lee","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03xs9yg50","id-type":"ROR","asserted-by":"publisher"}],"name":"Animal Genetic Resource Research Center, National Institute of Animal Science, Rural Development Administration, Hamyang 50000, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5229-7294","authenticated-orcid":false,"given":"Bongki","family":"Kim","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/0373nm262","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Animal Resources Science, Kongju National University, Yesan 32439, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"3132","published-online":{"date-parts":[[2022,5,31]]},"reference":[{"key":"key2.02610021124e+13_B1","doi-asserted-by":"crossref","first-page":"3","DOI":"10.4103\/1008-682X.165946","article-title":"Regulation of epithelial function, differentiation, and\n                        remodeling in the epididymis","volume":"18","author":"Breton","year":"2016","journal-title":"Asian J Androl."},{"key":"key2.02610021124e+13_B2","doi-asserted-by":"crossref","first-page":"576","DOI":"10.2164\/jandrol.111.012971","article-title":"Establishment of cell-cell cross talk in the epididymis: control\n                        of luminal acidification","volume":"32","author":"Shum","year":"2011","journal-title":"J Androl."},{"key":"key2.02610021124e+13_B3","doi-asserted-by":"crossref","first-page":"1753","DOI":"10.1242\/jeb.027284","article-title":"Regulation of luminal acidification in the male reproductive\n                        tract via cell\u2013cell crosstalk","volume":"212","author":"Shum","year":"2009","journal-title":"J Exp Biol."},{"key":"key2.02610021124e+13_B4","doi-asserted-by":"crossref","first-page":"463","DOI":"10.1111\/j.1745-7262.2007.00308.x","article-title":"Orchestration of occludins, claudins, catenins and cadherins as\n                        players involved in maintenance of the blood-epididymal barrier in animals\n                        and humans","volume":"9","author":"Cyr","year":"2007","journal-title":"Asian J Androl."},{"key":"key2.02610021124e+13_B5","doi-asserted-by":"crossref","first-page":"851","DOI":"10.1095\/biolreprod.110.087452","article-title":"The blood-testis and blood-epididymis barriers are more than just\n                        their tight junctions","volume":"84","author":"Mital","year":"2011","journal-title":"Biol Reprod."},{"key":"key2.02610021124e+13_B6","doi-asserted-by":"crossref","first-page":"237","DOI":"10.1007\/978-1-4614-4711-5_12","volume-title":"Biology and regulation of blood\u2010tissue barriers","author":"Fran\u00e7a","year":"2013"},{"key":"key2.02610021124e+13_B7","doi-asserted-by":"crossref","first-page":"308","DOI":"10.1093\/biolreprod\/3.3.308","article-title":"The blood-testis barrier in the rat and the physiological\n                        compartmentation of the seminiferous epithelium","volume":"3","author":"Dym","year":"1970","journal-title":"Biol Reprod."},{"key":"key2.02610021124e+13_B8","first-page":"105","article-title":"Electron microscopic observations on the structural components of\n                        the blood-testis barrier","volume":"10","author":"Fawcett","year":"1970","journal-title":"J Reprod Fertil Suppl."},{"key":"key2.02610021124e+13_B9","doi-asserted-by":"crossref","first-page":"991","DOI":"10.1095\/biolreprod30.4.991","article-title":"Morphological evidence for a blood-epididymis barrier and the\n                        effects of gossypol on its integrity","volume":"30","author":"Hoffer","year":"1984","journal-title":"Biol Reprod."},{"key":"key2.02610021124e+13_B10","doi-asserted-by":"crossref","first-page":"425","DOI":"10.1002\/j.1939-4640.1989.tb00132.x","article-title":"Ultrastructural studies on the development of the\n                        blood-epididymis barrier in immature rats","volume":"10","author":"Agarwal","year":"1989","journal-title":"J Androl."},{"key":"key2.02610021124e+13_B11","doi-asserted-by":"crossref","first-page":"193","DOI":"10.1071\/BI9860193","article-title":"The movement of fluids and substances in the\n                        testis","volume":"39","author":"Setchell","year":"1986","journal-title":"Aust J Biol Sci."},{"key":"key2.02610021124e+13_B12","first-page":"E1099","article-title":"Passage of leptin across the blood-testis barrier","volume":"276","author":"Banks","year":"1999","journal-title":"Am J Physiol."},{"key":"key2.02610021124e+13_B13","doi-asserted-by":"crossref","first-page":"16696","DOI":"10.1073\/pnas.0506084102","article-title":"Androgens regulate the permeability of the blood\u2013testis\n                        barrier","volume":"102","author":"Meng","year":"2005","journal-title":"Proc Natl Acad Sci USA"},{"key":"key2.02610021124e+13_B14","doi-asserted-by":"crossref","first-page":"1181","DOI":"10.1095\/biolreprod27.5.1181","article-title":"Rat testis and epididymis can transport [3H]\n                        3-O-methyl-D-glucose, [3H] inositol and [3H]\u03b1-aminoisobutyric acid\n                        across its epithelia in vivo","volume":"27","author":"Hinton","year":"1982","journal-title":"Biol Reprod."},{"key":"key2.02610021124e+13_B15","doi-asserted-by":"crossref","first-page":"288","DOI":"10.1095\/biolreprod37.2.288","article-title":"Neutral amino acid absorption by the rat\n                        epididymis","volume":"37","author":"Hinton","year":"1987","journal-title":"Biol Reprod."},{"key":"key2.02610021124e+13_B16","doi-asserted-by":"crossref","first-page":"625","DOI":"10.2164\/jandrol.111.012989","article-title":"Immunophysiology and pathology of inflammation in the testis and\n                        epididymis","volume":"32","author":"Hedger","year":"2011","journal-title":"J Androl."},{"key":"key2.02610021124e+13_B17","doi-asserted-by":"crossref","first-page":"755","DOI":"10.1083\/jcb.103.3.755","article-title":"Identification of ZO-1: a high molecular weight polypeptide\n                        associated with the tight junction (zonula occludens) in a variety of\n                        epithelia","volume":"103","author":"Stevenson","year":"1986","journal-title":"J Cell Biol."},{"key":"key2.02610021124e+13_B18","doi-asserted-by":"crossref","first-page":"3460","DOI":"10.1073\/pnas.88.8.3460","article-title":"Identification of a 160-kDa polypeptide that binds to the tight\n                        junction protein ZO-1","volume":"88","author":"Gumbiner","year":"1991","journal-title":"Proc Natl Acad Sci USA"},{"key":"key2.02610021124e+13_B19","doi-asserted-by":"crossref","first-page":"199","DOI":"10.1083\/jcb.141.1.199","article-title":"ZO-3, a novel member of the MAGUK protein family found at the\n                        tight junction, interacts with ZO-1 and occludin","volume":"141","author":"Haskins","year":"1998","journal-title":"J Cell Biol."},{"key":"key2.02610021124e+13_B20","doi-asserted-by":"crossref","first-page":"181","DOI":"10.1083\/jcb.138.1.181","article-title":"Involvement of ZO-1 in cadherin-based cell adhesion through its\n                        direct binding to \u03b1 catenin and actin filaments","volume":"138","author":"Itoh","year":"1997","journal-title":"J Cell Biol."},{"key":"key2.02610021124e+13_B21","doi-asserted-by":"crossref","first-page":"35179","DOI":"10.1074\/jbc.274.49.35179","article-title":"Protein interactions at the tight junction: Actin has multiple\n                        binding partners, and ZO-1 forms independent complexes with ZO-2 and\n                        ZO-3","volume":"274","author":"Wittchen","year":"1999","journal-title":"J Biol Chem."},{"key":"key2.02610021124e+13_B22","doi-asserted-by":"crossref","first-page":"1777","DOI":"10.1083\/jcb.123.6.1777","article-title":"Occludin: a novel integral membrane protein localizing at tight\n                        junctions","volume":"123","author":"Furuse","year":"1993","journal-title":"J Cell Biol."},{"key":"key2.02610021124e+13_B23","doi-asserted-by":"crossref","first-page":"1539","DOI":"10.1083\/jcb.141.7.1539","article-title":"Claudin-1 and -2: novel integral membrane proteins localizing at\n                        tight junctions with no sequence similarity to occludin","volume":"141","author":"Furuse","year":"1998","journal-title":"J Cell Biol."},{"key":"key2.02610021124e+13_B24","doi-asserted-by":"crossref","first-page":"403","DOI":"10.1146\/annurev.physiol.68.040104.131404","article-title":"Claudins and epithelial paracellular transport","volume":"68","author":"Van Itallie","year":"2006","journal-title":"Annu Rev Physiol."},{"key":"key2.02610021124e+13_B25","doi-asserted-by":"crossref","first-page":"1617","DOI":"10.1083\/jcb.127.6.1617","article-title":"Direct association of occludin with ZO-1 and its possible\n                        involvement in the localization of occludin at tight\n                        junctions","volume":"127","author":"Furuse","year":"1994","journal-title":"J Cell Biol."},{"key":"key2.02610021124e+13_B26","doi-asserted-by":"crossref","first-page":"1351","DOI":"10.1083\/jcb.147.6.1351","article-title":"Direct binding of three tight junction-associated MAGUKs, ZO-1,\n                        ZO-2, and ZO-3, with the COOH termini of claudins","volume":"147","author":"Itoh","year":"1999","journal-title":"J Cell Biol."},{"key":"key2.02610021124e+13_B27","doi-asserted-by":"crossref","first-page":"333","DOI":"10.1002\/ar.1091760309","article-title":"The normal development of the blood-testis barrier and the\n                        effects of clomiphene and estrogen treatment","volume":"176","author":"Vitale","year":"1973","journal-title":"Anat Rec."},{"key":"key2.02610021124e+13_B28","doi-asserted-by":"crossref","first-page":"177","DOI":"10.1016\/S0074-7696(08)60397-6","article-title":"Sertoli cell junctions: morphological and functional\n                        correlates","volume":"94","author":"Russell","year":"1985","journal-title":"Int Rev Cytol."},{"key":"key2.02610021124e+13_B29","doi-asserted-by":"crossref","first-page":"1169","DOI":"10.1530\/REP-06-0385","article-title":"Claudin-11 expression and localisation is regulated by androgens\n                        in rat Sertoli cells in vitro","volume":"133","author":"Kaitu\u2019u-Lino","year":"2007","journal-title":"Reproduction"},{"key":"key2.02610021124e+13_B30","doi-asserted-by":"crossref","first-page":"1532","DOI":"10.1210\/en.2004-0834","article-title":"Androgens and postmeiotic germ cells regulate claudin-11\n                        expression in rat Sertoli cells","volume":"146","author":"Florin","year":"2005","journal-title":"Endocrinology"},{"key":"key2.02610021124e+13_B31","doi-asserted-by":"crossref","first-page":"22","DOI":"10.1095\/biolreprod.115.134965","article-title":"The MAPK\/ERK-signaling pathway regulates the expression and\n                        distribution of tight junction proteins in the mouse proximal\n                        epididymis","volume":"94","author":"Kim","year":"2016","journal-title":"Biol Reprod."},{"key":"key2.02610021124e+13_B32","doi-asserted-by":"crossref","first-page":"190","DOI":"10.12750\/JARB.34.3.190","article-title":"The Expression and localization of ZO-1, claudin 1, and claudin 4\n                        in the pig epididymis","volume":"34","author":"Park","year":"2019","journal-title":"J Anim Reprod Biotechnol."},{"key":"key2.02610021124e+13_B33","doi-asserted-by":"crossref","first-page":"3697","DOI":"10.3892\/mmr.2016.5725","article-title":"Expression of claudins, occludin, junction adhesion molecule A\n                        and zona occludens 1 in canine organs","volume":"14","author":"Ahn","year":"2016","journal-title":"Mol Med Rep."},{"key":"key2.02610021124e+13_B34","article-title":"Localization and expression of zonula occludins-1 in the rabbit\n                        corneal epithelium following exposure to benzalkonium\n                        chloride","volume":"7","author":"Chen","year":"2012","journal-title":"PLOS ONE"},{"key":"key2.02610021124e+13_B35","doi-asserted-by":"crossref","first-page":"1679","DOI":"10.1098\/rstb.2010.0025","article-title":"Claudin and occludin expression and function in the seminiferous\n                        epithelium","volume":"365","author":"Morrow","year":"2010","journal-title":"Philos Trans R Soc B Biol Sci."},{"key":"key2.02610021124e+13_B36","doi-asserted-by":"crossref","first-page":"3815","DOI":"10.1210\/endo.140.8.6903","article-title":"Cellular immunolocalization of occludin during embryonic and\n                        postnatal development of the mouse testis and epididymis","volume":"140","author":"Cyr","year":"1999","journal-title":"Endocrinology"},{"key":"key2.02610021124e+13_B37","doi-asserted-by":"crossref","first-page":"580","DOI":"10.1002\/mrd.20467","article-title":"Identification of multiple claudins in the rat\n                        epididymis","volume":"73","author":"Gregory","year":"2006","journal-title":"Mol Reprod Dev."},{"key":"key2.02610021124e+13_B38","doi-asserted-by":"crossref","first-page":"1779526","DOI":"10.1080\/21688370.2020.1779526","article-title":"Distribution pattern of ZO-1 and claudins in the epididymis of\n                        vampire bats","volume":"8","author":"Castro","year":"2020","journal-title":"Tissue Barriers"},{"key":"key2.02610021124e+13_B39","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1007\/978-1-4615-0273-9_4","volume-title":"Introduction to mammalian reproduction","author":"Rodr\u00edguez","year":"2003"},{"key":"key2.02610021124e+13_B40","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1095\/biolreprod.114.123943","article-title":"Role of testicular luminal factors on basal cell elongation and\n                        proliferation in the mouse epididymis","volume":"92","author":"Kim","year":"2015","journal-title":"Biol Reprod."},{"key":"key2.02610021124e+13_B41","doi-asserted-by":"crossref","first-page":"854","DOI":"10.1210\/endo.142.2.7975","article-title":"Claudin-1 is not restricted to tight junctions in the rat\n                        epididymis","volume":"142","author":"Gregory","year":"2001","journal-title":"Endocrinology"},{"key":"key2.02610021124e+13_B42","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1530\/jrf.0.0230079","article-title":"The secretion of fluid by the testes of rats, rams and goats with\n                        some observations on the effect of age, cryptorchidism and\n                        hypophysectomy","volume":"23","author":"Setchell","year":"1970","journal-title":"J Reprod Fertil."},{"key":"key2.02610021124e+13_B43","doi-asserted-by":"crossref","first-page":"1273","DOI":"10.3390\/ani10081273","article-title":"Development and differentiation of epididymal epithelial cells in\n                        Korean native black goat","volume":"10","author":"Jeong","year":"2020","journal-title":"Animals"},{"key":"key2.02610021124e+13_B44","doi-asserted-by":"crossref","first-page":"5624","DOI":"10.1210\/en.2006-0138","article-title":"Androgen receptor in Sertoli cell is essential for germ cell\n                        nursery and junctional complex formation in mouse testes","volume":"147","author":"Wang","year":"2006","journal-title":"Endocrinology"},{"key":"key2.02610021124e+13_B45","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0014168","article-title":"Selective ablation of the androgen receptor in mouse Sertoli\n                        cells affects sertoli cell maturation, barrier formation and cytoskeletal\n                        development","volume":"5","author":"Willems","year":"2010","journal-title":"PLOS ONE"},{"key":"key2.02610021124e+13_B46","doi-asserted-by":"crossref","first-page":"507","DOI":"10.1111\/j.1365-2605.2009.00964.x","article-title":"Early effects of Sertoli cell-selective androgen receptor\n                        ablation on testicular gene expression","volume":"33","author":"Willems","year":"2010","journal-title":"Int J Androl."},{"key":"key2.02610021124e+13_B47","doi-asserted-by":"crossref","first-page":"1537","DOI":"10.1098\/rstb.2009.0117","article-title":"Androgens and spermatogenesis: lessons from transgenic mouse\n                        models","volume":"365","author":"Verhoeven","year":"2010","journal-title":"Philos Trans R Soc B Biol Sci."},{"key":"key2.02610021124e+13_B48","doi-asserted-by":"crossref","first-page":"119","DOI":"10.1210\/er.2008-0025","article-title":"Androgen receptor roles in spermatogenesis and fertility: lessons\n                        from testicular cell-specific androgen receptor knockout\n                        mice","volume":"30","author":"Wang","year":"2009","journal-title":"Endocr Rev."},{"key":"key2.02610021124e+13_B49","doi-asserted-by":"crossref","first-page":"1517","DOI":"10.1098\/rstb.2009.0235","article-title":"Oestrogens and spermatogenesis","volume":"365","author":"Carreau","year":"2010","journal-title":"Philos Trans R Soc B Biol Sci."},{"key":"key2.02610021124e+13_B50","doi-asserted-by":"crossref","first-page":"207","DOI":"10.1095\/biolreprod.110.087353","article-title":"Estrogen, efferent ductules, and the epididymis","volume":"84","author":"Joseph","year":"2011","journal-title":"Biol Reprod."},{"key":"key2.02610021124e+13_B51","doi-asserted-by":"crossref","first-page":"509","DOI":"10.1038\/37352","article-title":"A role for oestrogens in the male reproductive\n                        system","volume":"390","author":"Hess","year":"1997","journal-title":"Nature"},{"key":"key2.02610021124e+13_B52","doi-asserted-by":"crossref","first-page":"413","DOI":"10.1002\/j.1939-4640.2001.tb02197.x","article-title":"Neonatally administered diethylstilbestrol retards the\n                        development of the blood-testis barrier in the rat","volume":"22","author":"Toyama","year":"2001","journal-title":"J Androl."},{"key":"key2.02610021124e+13_B53","doi-asserted-by":"crossref","first-page":"2302","DOI":"10.1016\/j.biocel.2009.05.016","article-title":"Disruption of the blood-testis barrier integrity by bisphenol A\n                        in vitro: is this a suitable model for studying blood-testis barrier\n                        dynamics?","volume":"41","author":"Li","year":"2009","journal-title":"Int J Biochem Cell Biol."}],"container-title":["Journal of Animal Science and Technology"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/www.ejast.org\/download\/download_pdf?doi=10.5187\/jast.2022.e13","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/www.ejast.org\/download\/download_pdf?doi=10.5187\/jast.2022.e13","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T02:48:12Z","timestamp":1790909292000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.ejast.org\/archive\/view_article?doi=10.5187\/jast.2022.e13"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,5]]},"references-count":53,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2022,5,31]]},"published-print":{"date-parts":[[2022,5]]}},"alternative-id":["10.5187\/jast.2022.e13"],"URL":"https:\/\/doi.org\/10.5187\/jast.2022.e13","relation":{},"ISSN":["2672-0191","2055-0391"],"issn-type":[{"value":"2672-0191","type":"print"},{"value":"2055-0391","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,5]]},"assertion":[{"value":"2022-01-05","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication 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