{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T02:55:41Z","timestamp":1790909741523,"version":"4.1.0"},"reference-count":34,"publisher":"Korean Society of Animal Science and Technology","issue":"2","license":[{"start":{"date-parts":[[2022,3,1]],"date-time":"2022-03-01T00:00:00Z","timestamp":1646092800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0"}],"funder":[{"name":"Shaoguan City Science and Technology","award":["210728094530484"],"award-info":[{"award-number":["210728094530484"]}]},{"name":"Scientific Research Foundation of Shao-guan\n                                University","award":["99000639"],"award-info":[{"award-number":["99000639"]}]},{"name":"Guangdong Modern Agricultural Research System","award":["2020KJ126"],"award-info":[{"award-number":["2020KJ126"]}]}],"content-domain":{"domain":["www.ejast.org"],"crossmark-restriction":true},"short-container-title":["J Anim Sci Technol"],"published-print":{"date-parts":[[2022,3]]},"abstract":"<jats:p>In this study, we used more reliable experimental materials and methods to detect\n                    the effects of osteopontin (OPN) on boar sperm in vitro\n                    capacitation, acrosome reaction, and fertilization efficiency. We reorganized\n                    and obtained the OPN protein of the porcine source. Immunofluorescence and\n                    Western blot show the localization and expression of the OPN protein before and\n                    after sperm capacitation. To determine whether OPN can affect sperm during sperm\n                    capacitation, we examined cyclic adenosine monophosphate (cAMP) concentrations\n                    after sperm capacitation, and the results showed that OPN significantly\n                    increased the cAMP concentration in sperm (p &lt; 0.05).\n                    Flow cytometry showed that 0.1 \u03bcg\/mL OPN-treated sperm had better\n                    acrosome reaction ability. In vitro fertilization (IVF) showed\n                    that 0.1 \u03bcg\/mL OPN significantly increased the rate of embryo division.\n                    In conclusion, this study found that 0.1 \u03bcg\/mL porcine OPN protein can\n                    significantly improve porcine capacitated sperm motility, cAMP concentration\n                    after capacitation sperm, acrosome reaction ability, and embryo division during\n                    IVF and provides new clues to explore the mechanism of OPN\u2019s function on\n                    sperm.<\/jats:p>","DOI":"10.5187\/jast.2022.e15","type":"journal-article","created":{"date-parts":[[2022,3,27]],"date-time":"2022-03-27T21:51:53Z","timestamp":1648417913000},"page":"235-246","update-policy":"https:\/\/doi.org\/10.5187\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Osteopontin enhances sperm capacitation and in vitro\n                    fertilization efficiency in boars"],"prefix":"10.5187","volume":"64","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2610-4003","authenticated-orcid":false,"given":"Yun","family":"Chen","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/0286g6711","id-type":"ROR","asserted-by":"publisher"}],"name":"Henry Fok College of Biology and Agriculture, Shaoguan University, Shaoguan 512000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6049-1970","authenticated-orcid":false,"given":"Kai","family":"Wang","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/05v9jqt67","id-type":"ROR","asserted-by":"publisher"}],"name":"National Engineering Research Center for Breeding Swine Industry, Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding, College of Animal Science, South China Agricultural University, Guangzhou 510642, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6757-9465","authenticated-orcid":false,"given":"Shouquan","family":"Zhang","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/05v9jqt67","id-type":"ROR","asserted-by":"publisher"}],"name":"National Engineering Research Center for Breeding Swine Industry, Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding, College of Animal Science, South China Agricultural University, Guangzhou 510642, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"3132","published-online":{"date-parts":[[2022,3,31]]},"reference":[{"key":"key2.0261002112301e+13_B1","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1007\/978-1-4614-8887-3_7","article-title":"Impact of swine reproductive technologies on pig and global food\n                        production","volume":"752","author":"Knox","year":"2014","journal-title":"Adv Exp Med Biol."},{"key":"key2.0261002112301e+13_B2","doi-asserted-by":"crossref","first-page":"2171","DOI":"10.1534\/g3.117.042671","article-title":"A pathway-centered analysis of pig domestication and breeding in\n                        Eurasia","volume":"7","author":"Leno-Colorado","year":"2017","journal-title":"G3 (Bethesda)"},{"key":"key2.0261002112301e+13_B3","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1016\/j.anireprosci.2018.02.014","article-title":"Semen evaluation and in vivo fertility in a Northern Italian pig\n                        farm: can advanced statistical approaches compensate for low sample size? An\n                        observational study","volume":"192","author":"Elmi","year":"2018","journal-title":"Anim Reprod Sci."},{"key":"key2.0261002112301e+13_B4","doi-asserted-by":"crossref","first-page":"719","DOI":"10.1071\/RD07035","article-title":"Novel gamete storage","volume":"19","author":"Dinnyes","year":"2007","journal-title":"Reprod Fertil Dev."},{"key":"key2.0261002112301e+13_B5","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1002\/mrd.23290","article-title":"Porcine IVF embryo development and estrogen receptors are\n                        influenced by the concentration of percoll gradients during sperm\n                        selection","volume":"87","author":"Ohlweiler","year":"2020","journal-title":"Mol Reprod Dev."},{"key":"key2.0261002112301e+13_B6","doi-asserted-by":"crossref","first-page":"40","DOI":"10.1111\/j.1439-0531.2010.01623.x","article-title":"Advances in swine in vitro embryo production\n                        technologies","volume":"45","author":"Gil","year":"2010","journal-title":"Reprod Domest Anim."},{"key":"key2.0261002112301e+13_B7","doi-asserted-by":"crossref","first-page":"212","DOI":"10.1016\/j.theriogenology.2013.04.017","article-title":"Osteopontin improves sperm capacitation and in vitro\n                        fertilization efficiency in buffalo (Bubalus bubalis)","volume":"80","author":"Boccia","year":"2013","journal-title":"Theriogenology"},{"key":"key2.0261002112301e+13_B8","doi-asserted-by":"crossref","first-page":"450","DOI":"10.1016\/j.theriogenology.2008.08.012","article-title":"Effect of osteopontin (OPN) on in vitro embryo development in\n                        cattle","volume":"71","author":"Monaco","year":"2009","journal-title":"Theriogenology"},{"key":"key2.0261002112301e+13_B9","doi-asserted-by":"crossref","first-page":"468","DOI":"10.1016\/j.theriogenology.2006.08.013","article-title":"Influence of arginine-glycine-aspartic acid (RGD), integrins\n                        (\u03b1V and \u03b15) and osteopontin on bovine sperm\u2013egg\n                        binding, and fertilization in vitro","volume":"67","author":"Gon\u00e7alves","year":"2007","journal-title":"Theriogenology"},{"key":"key2.0261002112301e+13_B10","doi-asserted-by":"crossref","first-page":"201","DOI":"10.2164\/jandrol.05089","article-title":"Identification of proteins in the accessory sex gland fluid\n                        associated with fertility indexes of dairy bulls: a proteomic\n                        approach","volume":"27","author":"Moura","year":"2006","journal-title":"J Androl."},{"key":"key2.0261002112301e+13_B11","doi-asserted-by":"crossref","first-page":"538","DOI":"10.1111\/j.1439-0531.2006.00710.x","article-title":"Evidence for effects of testis and epididymis expressed genes on\n                        sperm quality and boar fertility traits","volume":"41","author":"Lin","year":"2006","journal-title":"Reprod Domest 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Chem."},{"key":"key2.0261002112301e+13_B32","doi-asserted-by":"crossref","first-page":"9699","DOI":"10.1016\/S0021-9258(17)39295-5","article-title":"Sodium bicarbonate in seminal plasma stimulates the motility of\n                        mammalian spermatozoa through direct activation of adenylate\n                        cyclase","volume":"260","author":"Okamura","year":"1985","journal-title":"J Biol Chem."},{"key":"key2.0261002112301e+13_B33","doi-asserted-by":"crossref","first-page":"111","DOI":"10.1016\/j.mce.2017.11.008","article-title":"Shedding light on the role of cAMP in mammalian sperm\n                        physiology","volume":"468","author":"Balbach","year":"2018","journal-title":"Mol Cell Endocrinol."},{"key":"key2.0261002112301e+13_B34","doi-asserted-by":"crossref","first-page":"10119","DOI":"10.1093\/nar\/17.23.10119","article-title":"Full length cDNA sequence of porcine secreted phosphoprotein-I\n                        (SPP-I, osteopontin)","volume":"17","author":"Wrana","year":"1989","journal-title":"Nucleic Acids Res."}],"container-title":["Journal of Animal Science and Technology"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/www.ejast.org\/download\/download_pdf?doi=10.5187\/jast.2022.e15","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/www.ejast.org\/download\/download_pdf?doi=10.5187\/jast.2022.e15","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T02:44:33Z","timestamp":1790909073000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.ejast.org\/archive\/view_article?doi=10.5187\/jast.2022.e15"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,3]]},"references-count":34,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2022,3,31]]},"published-print":{"date-parts":[[2022,3]]}},"alternative-id":["10.5187\/jast.2022.e15"],"URL":"https:\/\/doi.org\/10.5187\/jast.2022.e15","relation":{},"ISSN":["2672-0191","2055-0391"],"issn-type":[{"value":"2672-0191","type":"print"},{"value":"2055-0391","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,3]]},"assertion":[{"value":"2021-11-29","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication 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