{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T17:15:12Z","timestamp":1792170912362,"version":"4.4.0"},"reference-count":28,"publisher":"Korean Society of Animal Science and Technology","issue":"6","license":[{"start":{"date-parts":[[2022,11,1]],"date-time":"2022-11-01T00:00:00Z","timestamp":1667260800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0"}],"funder":[{"name":"National Institute of Animal Science","award":["PJ01422201"],"award-info":[{"award-number":["PJ01422201"]}]}],"content-domain":{"domain":["www.ejast.org"],"crossmark-restriction":true},"short-container-title":["J Anim Sci Technol"],"published-print":{"date-parts":[[2022,11]]},"abstract":"<jats:p>Recently, we reported the robust in vitro three-dimensional (3D)\n                    expansion of intestinal organoids derived from adult bovine (&gt; 24 months)\n                    samples. The present study aimed to establish an in vitro 3D\n                    system for the cultivation of intestinal organoids derived from growing cattle\n                    (12 months old) for practical use as a potential alternative to in\n                        vivo systems for various purposes. However, very few studies on the\n                    functional characterization and 3D expansion of adult stem cells from livestock\n                    species compared to those from other species are available. In this study,\n                    intestinal crypts, including intestinal stem cells, from the small intestines\n                    (ileum and jejunum) of growing cattle were isolated and long-term 3D cultures\n                    were successfully established using a scaffold-based method. Furthermore, we\n                    generated an apical-out intestinal organoid derived from growing cattle.\n                    Interestingly, intestinal organoids derived from the ileum, but not the jejunum,\n                    could be expanded without losing the ability to recapitulate crypts, and these\n                    organoids specifically expressed several specific markers of intestinal stem\n                    cells and the intestinal epithelium. Furthermore, these organoids exhibited key\n                    functionality with regard to high permeability for compounds up to 4 kDa in size\n                    (e.g., fluorescein isothiocyanate [FITC]-dextran), indicating that apical-out\n                    intestinal organoids are better than other models. Collectively, these results\n                    indicate the establishment of growing cattle-derived intestinal organoids and\n                    subsequent generation of apical-out intestinal organoids. These organoids may be\n                    valuable tools and potential alternatives to in vivo systems\n                    for examining host-pathogen interactions involving epithelial cells, such as\n                    enteric virus infection and nutrient absorption, and may be used for various\n                    purposes.<\/jats:p>","DOI":"10.5187\/jast.2022.e70","type":"journal-article","created":{"date-parts":[[2022,10,21]],"date-time":"2022-10-21T03:05:25Z","timestamp":1666321525000},"page":"1105-1116","update-policy":"https:\/\/doi.org\/10.5187\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Establishment of intestinal organoids from small intestine of growing\n                    cattle (12 months old)"],"prefix":"10.5187","volume":"64","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6487-1583","authenticated-orcid":false,"given":"Kang Won","family":"Park","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03xs9yg50","id-type":"ROR","asserted-by":"publisher"}],"name":"Animal Biotechnology Division, National Institute of Animal Science, Rural Development Administration, Wanju 55365, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4162-4410","authenticated-orcid":false,"given":"Hyeon","family":"Yang","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03xs9yg50","id-type":"ROR","asserted-by":"publisher"}],"name":"Animal Biotechnology Division, National Institute of Animal Science, Rural Development Administration, Wanju 55365, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8237-6447","authenticated-orcid":false,"given":"Min Gook","family":"Lee","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03xs9yg50","id-type":"ROR","asserted-by":"publisher"}],"name":"Animal Biotechnology Division, National Institute of Animal Science, Rural Development Administration, Wanju 55365, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0887-8200","authenticated-orcid":false,"given":"Sun A","family":"Ock","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03xs9yg50","id-type":"ROR","asserted-by":"publisher"}],"name":"Animal Biotechnology Division, National Institute of Animal Science, Rural Development Administration, Wanju 55365, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5008-4626","authenticated-orcid":false,"given":"Hayeon","family":"Wi","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03xs9yg50","id-type":"ROR","asserted-by":"publisher"}],"name":"Animal Biotechnology Division, National Institute of Animal Science, Rural Development Administration, Wanju 55365, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2447-0392","authenticated-orcid":false,"given":"Poongyeon","family":"Lee","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03xs9yg50","id-type":"ROR","asserted-by":"publisher"}],"name":"Animal Biotechnology Division, National Institute of Animal Science, Rural Development Administration, Wanju 55365, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2949-3075","authenticated-orcid":false,"given":"In-Sul","family":"Hwang","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/00hj8s172","id-type":"ROR","asserted-by":"publisher"}],"name":"Columbia Center for Translational Immunology, Columbia University Irving Medical Center, Columbia University, New York, NY 10032, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8542-9193","authenticated-orcid":false,"given":"Jae Gyu","family":"Yoo","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03xs9yg50","id-type":"ROR","asserted-by":"publisher"}],"name":"Animal Biotechnology Division, National Institute of Animal Science, Rural Development Administration, Wanju 55365, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2786-8814","authenticated-orcid":false,"given":"Choon-Keun","family":"Park","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01mh5ph17","id-type":"ROR","asserted-by":"publisher"}],"name":"College of Animal Life Sciences, Kangwon National University, Chuncheon 24341, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0537-6205","authenticated-orcid":false,"given":"Bo Ram","family":"Lee","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03xs9yg50","id-type":"ROR","asserted-by":"publisher"}],"name":"Animal Biotechnology Division, National Institute of Animal Science, Rural Development Administration, Wanju 55365, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"3132","published-online":{"date-parts":[[2022,11,30]]},"reference":[{"key":"key2.026100211255e+13_B1","doi-asserted-by":"crossref","first-page":"83","DOI":"10.14397\/jals.2021.55.5.83","article-title":"Recent progress and future perspectives on intestinal organoids\n                        in livestock","volume":"55","author":"Haq","year":"2021","journal-title":"J Agric Life Sci."},{"key":"key2.026100211255e+13_B2","doi-asserted-by":"crossref","first-page":"2115","DOI":"10.3390\/ani11072115","article-title":"Robust three-dimensional (3D) expansion of bovine intestinal\n                        organoids: an in vitro model as a potential alternative to an in vivo\n                        system","volume":"11","author":"Lee","year":"2021","journal-title":"Animals."},{"key":"key2.026100211255e+13_B3","doi-asserted-by":"crossref","first-page":"745","DOI":"10.1016\/j.tcb.2011.09.005","article-title":"From 3D cell culture 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Dis."},{"key":"key2.026100211255e+13_B6","doi-asserted-by":"crossref","first-page":"241","DOI":"10.1111\/evj.12734","article-title":"Advanced three-dimensional culture of equine intestinal\n                        epithelial stem cells","volume":"50","author":"Stewart","year":"2018","journal-title":"Equine Vet J."},{"key":"key2.026100211255e+13_B7","doi-asserted-by":"crossref","first-page":"641","DOI":"10.1007\/s13770-020-00278-0","article-title":"Evaluation of intestinal epithelial barrier function in\n                        inflammatory bowel diseases using murine intestinal\n                        organoids","volume":"17","author":"Rallabandi","year":"2020","journal-title":"Tissue Eng Regen Med."},{"key":"key2.026100211255e+13_B8","doi-asserted-by":"crossref","first-page":"e01006","DOI":"10.1128\/JVI.01006-20","article-title":"Next-generation porcine intestinal organoids: an apical-out\n                        organoid model for swine enteric virus infection and immune response\n  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intestinal stem cells in mice","volume":"158","author":"Chen","year":"2020","journal-title":"Gastroenterology"},{"key":"key2.026100211255e+13_B18","doi-asserted-by":"crossref","first-page":"G1108","DOI":"10.1152\/ajpgi.00004.2009","article-title":"Distinct SOX9 levels differentially mark stem\/progenitor\n                        populations and enteroendocrine cells of the small intestine\n                        epithelium","volume":"296","author":"Formeister","year":"2009","journal-title":"Am J Physiol Gastrointest Liver Physiol."},{"key":"key2.026100211255e+13_B19","doi-asserted-by":"crossref","first-page":"3805","DOI":"10.1038\/s41467-020-17535-4","article-title":"Collective cell mechanics of epithelial shells with organoid-like\n                        morphologies","volume":"11","author":"Rozman","year":"2020","journal-title":"Nat Commun."},{"key":"key2.026100211255e+13_B20","doi-asserted-by":"crossref","first-page":"52","DOI":"10.1111\/j.1469-7580.2008.00925.x","article-title":"Small intestinal stem cell markers","volume":"213","author":"Montgomery","year":"2008","journal-title":"J Anat."},{"key":"key2.026100211255e+13_B21","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0256143","article-title":"Intestinal organoid-based 2D monolayers mimic physiological and\n                        pathophysiological properties of the pig intestine","volume":"16","author":"Hoffmann","year":"2021","journal-title":"PLOS ONE"},{"key":"key2.026100211255e+13_B22","doi-asserted-by":"crossref","first-page":"409","DOI":"10.1007\/s00441-018-2924-9","article-title":"Developing a 3D intestinal epithelium model for livestock\n                        species","volume":"375","author":"Derricott","year":"2019","journal-title":"Cell Tissue Res."},{"key":"key2.026100211255e+13_B23","doi-asserted-by":"crossref","DOI":"10.14814\/phy2.12147","article-title":"Salmonella-infected crypt-derived intestinal organoid culture\n                        system for host\u2013bacterial interactions","volume":"2","author":"Zhang","year":"2014","journal-title":"Physiol Rep."},{"key":"key2.026100211255e+13_B24","doi-asserted-by":"crossref","first-page":"2926","DOI":"10.1128\/IAI.00161-15","article-title":"Interaction of Salmonella enterica serovar Typhimurium with\n                        intestinal organoids derived from human induced pluripotent stem\n                        cells","volume":"83","author":"Forbester","year":"2015","journal-title":"Infect Immun."},{"key":"key2.026100211255e+13_B25","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1128\/JVI.01930-15","article-title":"Human intestinal enteroids: a new model to study human rotavirus\n                        infection, host restriction, and pathophysiology","volume":"90","author":"Saxena","year":"2016","journal-title":"J Virol."},{"key":"key2.026100211255e+13_B26","doi-asserted-by":"crossref","first-page":"195","DOI":"10.1016\/j.biomaterials.2018.12.006","article-title":"Intestinal organoids: a new paradigm for engineering intestinal\n                        epithelium in vitro","volume":"194","author":"Rahmani","year":"2019","journal-title":"Biomaterials."},{"key":"key2.026100211255e+13_B27","doi-asserted-by":"crossref","first-page":"1185","DOI":"10.3390\/nu12041185","article-title":"Intestinal permeability, inflammation and the role of\n                        nutrients","volume":"12","author":"Farr\u00e9","year":"2020","journal-title":"Nutrients."},{"key":"key2.026100211255e+13_B28","doi-asserted-by":"crossref","first-page":"16831","DOI":"10.1038\/srep16831","article-title":"Intestinal organoids for assessing nutrient transport, sensing\n                        and incretin secretion","volume":"5","author":"Zietek","year":"2015","journal-title":"Sci Rep."}],"container-title":["Journal of Animal Science and Technology"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/www.ejast.org\/download\/download_pdf?doi=10.5187\/jast.2022.e70","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/www.ejast.org\/download\/download_pdf?doi=10.5187\/jast.2022.e70","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T02:57:41Z","timestamp":1790909861000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.ejast.org\/archive\/view_article?doi=10.5187\/jast.2022.e70"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,11]]},"references-count":28,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2022,11,30]]},"published-print":{"date-parts":[[2022,11]]}},"alternative-id":["10.5187\/jast.2022.e70"],"URL":"https:\/\/doi.org\/10.5187\/jast.2022.e70","relation":{},"ISSN":["2672-0191","2055-0391"],"issn-type":[{"value":"2672-0191","type":"print"},{"value":"2055-0391","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,11]]},"assertion":[{"value":"2022-05-25","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-08-23","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}}]}}