{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T03:53:22Z","timestamp":1790913202686,"version":"4.1.0"},"reference-count":23,"publisher":"Korean Society of Animal Science and Technology","issue":"6","license":[{"start":{"date-parts":[[2022,11,1]],"date-time":"2022-11-01T00:00:00Z","timestamp":1667260800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0"}],"funder":[{"name":"Gyeongsangbuk-do","award":["2021-207"],"award-info":[{"award-number":["2021-207"]}]}],"content-domain":{"domain":["www.ejast.org"],"crossmark-restriction":true},"short-container-title":["J Anim Sci Technol"],"published-print":{"date-parts":[[2022,11]]},"abstract":"<jats:p>Identifying effective biomarkers for the diagnosis of male fertility is crucial\n                    for improving animal production and treating male infertility in humans.\n                    Ras-related proteins (Rab) are associated with morphological and motion\n                    kinematic functions in spermatozoa. Moreover, Rab2A, a Rab protein, is a\n                    possible male fertility-related biomarker. The present study was designed to\n                    identify additional fertility-related biomarkers among the various Rab proteins.\n                    First, the expression of Rab proteins (Rab3A, 4, 5, 8A, 9, 14, 25, 27A, and 34A)\n                    from 31 duroc boar spermatozoa was measured before and after capacitation;\n                    correlation between Rab protein expression and litter size was evaluated by\n                    statistical analysis. The results showed that the expression of Rab3A, 4, 5, 8A,\n                    9, and 25 before capacitation and Rab3A, 4, 5, 8A, 9, and 14 after capacitation\n                    were negatively correlated with litter size. Moreover, depending on the cut-off\n                    values calculated by receiver operating curves, an increase in litter size was\n                    observed when evaluating the ability of the Rab proteins to forecast litter\n                    size. Therefore, we suggest that Rab proteins may be potential fertility-related\n                    biomarkers that could help select superior sires in the livestock industry.<\/jats:p>","DOI":"10.5187\/jast.2022.e83","type":"journal-article","created":{"date-parts":[[2022,10,21]],"date-time":"2022-10-21T03:05:25Z","timestamp":1666321525000},"page":"1024-1034","update-policy":"https:\/\/doi.org\/10.5187\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Prediction of male fertility using Ras-related\n                    proteins"],"prefix":"10.5187","volume":"64","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7030-7767","authenticated-orcid":false,"given":"Jeong-Won","family":"Bae","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/040c17130","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Animal Science and Biotechnology, Kyungpook National University, Sangju 37224, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1776-9759","authenticated-orcid":false,"given":"Ju-Mi","family":"Hwang","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/040c17130","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Animal Science and Biotechnology, Kyungpook National University, Sangju 37224, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0848-7189","authenticated-orcid":false,"given":"Woo-Sung","family":"Kwon","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/040c17130","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Animal Science and Biotechnology, Kyungpook National University, Sangju 37224, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"3132","published-online":{"date-parts":[[2022,11,30]]},"reference":[{"key":"key2.0261002112539e+13_B1","doi-asserted-by":"crossref","first-page":"15654","DOI":"10.1038\/srep15654","article-title":"Increased male fertility using fertility-related\n                        biomarkers","volume":"5","author":"Kwon","year":"2015","journal-title":"Sci Rep."},{"key":"key2.0261002112539e+13_B2","doi-asserted-by":"crossref","first-page":"1230","DOI":"10.1074\/mcp.M114.045369","article-title":"Discovery of predictive biomarkers for litter size in boar\n                        spermatozoa","volume":"14","author":"Kwon","year":"2015","journal-title":"Mol Cell Proteomics."},{"key":"key2.0261002112539e+13_B3","doi-asserted-by":"crossref","first-page":"42","DOI":"10.1186\/s40104-022-00689-0","article-title":"Heat shock protein family D member 1 in boar spermatozoa is\n                        strongly related to the litter size of inseminated sows","volume":"13","author":"Pang","year":"2022","journal-title":"J Anim Sci Biotechnol."},{"key":"key2.0261002112539e+13_B4","doi-asserted-by":"crossref","first-page":"4093","DOI":"10.1007\/s00018-012-1021-6","article-title":"Are Rab proteins the link between Golgi organization and membrane\n                        trafficking?","volume":"69","author":"Liu","year":"2012","journal-title":"Cell Mol Life Sci."},{"key":"key2.0261002112539e+13_B5","doi-asserted-by":"crossref","first-page":"100617","DOI":"10.1016\/j.repbio.2022.100617","article-title":"Ras-related proteins (Rab) play significant roles in sperm\n                        motility and capacitation status","volume":"22","author":"Bae","year":"2022","journal-title":"Reprod Biol."},{"key":"key2.0261002112539e+13_B6","doi-asserted-by":"crossref","first-page":"356","DOI":"10.1016\/j.repbio.2019.10.001","article-title":"Ras-related proteins (Rab) are key proteins related to male\n                        fertility following a unique activation mechanism","volume":"19","author":"Bae","year":"2019","journal-title":"Reprod Biol."},{"key":"key2.0261002112539e+13_B7","doi-asserted-by":"crossref","first-page":"E2057","DOI":"10.1073\/pnas.1121173109","article-title":"Rab27 and Rab3 sequentially regulate human sperm dense-core\n                        granule exocytosis","volume":"109","author":"Bustos","year":"2012","journal-title":"Proc Natl Acad Sci USA"},{"key":"key2.0261002112539e+13_B8","doi-asserted-by":"crossref","first-page":"590","DOI":"10.1095\/biolreprod.108.071647","article-title":"RAB13 participates in ectoplasmic specialization dynamics in the\n                        rat testis","volume":"80","author":"Mruk","year":"2009","journal-title":"Biol Reprod."},{"key":"key2.0261002112539e+13_B9","volume-title":"The swine A.I. book: a field and laboratory technicians\u2019 guide to\n                        artificial insemination in swine.","author":"Almond","year":"1998"},{"key":"key2.0261002112539e+13_B10","doi-asserted-by":"crossref","first-page":"225","DOI":"10.1016\/S0378-4320(01)00095-1","article-title":"Effect of birth litter size, birth parity number, growth rate,\n                        backfat thickness and age at first mating of gilts on their reproductive\n                        performance as sows","volume":"66","author":"Tummaruk","year":"2001","journal-title":"Anim Reprod Sci."},{"key":"key2.0261002112539e+13_B11","doi-asserted-by":"crossref","first-page":"842","DOI":"10.5713\/ajas.17.0760","article-title":"Applications of capacitation status for litter size enhancement\n                        in various pig breeds","volume":"31","author":"Kwon","year":"2018","journal-title":"Asian-Australas J Anim Sci."},{"key":"key2.0261002112539e+13_B12","doi-asserted-by":"crossref","first-page":"552","DOI":"10.1111\/andr.12020","article-title":"Improving litter size by boar spermatozoa: application of\n                        combined H33258\/CTC staining in field trial with artificial\n                        insemination","volume":"3","author":"Kwon","year":"2015","journal-title":"Andrology."},{"key":"key2.0261002112539e+13_B13","doi-asserted-by":"crossref","first-page":"568","DOI":"10.1111\/andr.12332","article-title":"Comparison of markers predicting litter size in different pig\n                        breeds","volume":"5","author":"Kwon","year":"2017","journal-title":"Andrology."},{"key":"key2.0261002112539e+13_B14","doi-asserted-by":"crossref","first-page":"897","DOI":"10.1186\/1471-2164-15-897","article-title":"A comprehensive proteomic approach to identifying capacitation\n                        related proteins in boar spermatozoa","volume":"15","author":"Kwon","year":"2014","journal-title":"BMC Genomics."},{"key":"key2.0261002112539e+13_B15","doi-asserted-by":"crossref","first-page":"248","DOI":"10.1016\/0003-2697(76)90527-3","article-title":"A rapid and sensitive method for the quantitation of microgram\n                        quantities of protein utilizing the principle of protein-dye\n                        binding","volume":"72","author":"Bradford","year":"1976","journal-title":"Anal Biochem."},{"key":"key2.0261002112539e+13_B16","doi-asserted-by":"crossref","first-page":"66","DOI":"10.1080\/21541248.2017.1384087","article-title":"Rab proteins as major determinants of the Golgi complex\n                        structure","volume":"9","author":"Goud","year":"2018","journal-title":"Small GTPases."},{"key":"key2.0261002112539e+13_B17","doi-asserted-by":"crossref","first-page":"415","DOI":"10.1538\/expanim.15-0016","article-title":"Expression and intracellular localization of TBC1D9, a Rab\n                        GTPase-accelerating protein, in mouse testes","volume":"64","author":"Nakamura","year":"2015","journal-title":"Exp Anim."},{"key":"key2.0261002112539e+13_B18","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1006\/excr.2000.5119","article-title":"Membrane trafficking machinery components associated with the\n                        mammalian acrosome during spermiogenesis","volume":"267","author":"Ramalho-Santos","year":"2001","journal-title":"Exp Cell Res."},{"key":"key2.0261002112539e+13_B19","doi-asserted-by":"crossref","first-page":"V","DOI":"10.1016\/S0079-6336(11)80025-2","article-title":"Ultrastructural distribution of NADPase within the Golgi\n                        apparatus and lysosomes of mammalian cells","volume":"21","author":"Smith","year":"1990","journal-title":"Prog Histochem Cytochem"},{"key":"key2.0261002112539e+13_B20","doi-asserted-by":"crossref","first-page":"4121","DOI":"10.1096\/fj.06-7716com","article-title":"Membrane-permeant Rab3A triggers acrosomal exocytosis in living\n                        human sperm","volume":"21","author":"Lopez","year":"2007","journal-title":"FASEB J."},{"key":"key2.0261002112539e+13_B21","first-page":"43","article-title":"Epac, Rap and Rab3 act in concert to mobilize calcium from\n                        sperm\u2019s acrosome during exocytosis","volume":"12","author":"Ruete","year":"2014","journal-title":"Cell Commun Signal."},{"key":"key2.0261002112539e+13_B22","doi-asserted-by":"crossref","first-page":"126","DOI":"10.1016\/S0014-5793(02)03102-2","article-title":"Rab3A and calmodulin regulate acrosomal exocytosis by mechanisms\n                        that do not require a direct interaction","volume":"525","author":"Yunes","year":"2002","journal-title":"FEBS Lett."},{"key":"key2.0261002112539e+13_B23","doi-asserted-by":"crossref","first-page":"1084","DOI":"10.1095\/biolreprod62.4.1084","article-title":"Rab3A triggers the acrosome reaction in permeabilized human\n                        spermatozoa","volume":"62","author":"Yunes","year":"2000","journal-title":"Biol Reprod."}],"container-title":["Journal of Animal Science and Technology"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/www.ejast.org\/download\/download_pdf?doi=10.5187\/jast.2022.e83","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/www.ejast.org\/download\/download_pdf?doi=10.5187\/jast.2022.e83","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T02:56:33Z","timestamp":1790909793000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.ejast.org\/archive\/view_article?doi=10.5187\/jast.2022.e83"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,11]]},"references-count":23,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2022,11,30]]},"published-print":{"date-parts":[[2022,11]]}},"alternative-id":["10.5187\/jast.2022.e83"],"URL":"https:\/\/doi.org\/10.5187\/jast.2022.e83","relation":{},"ISSN":["2672-0191","2055-0391"],"issn-type":[{"value":"2672-0191","type":"print"},{"value":"2055-0391","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,11]]},"assertion":[{"value":"2022-08-09","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-10-04","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}}]}}