{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T03:53:08Z","timestamp":1790913188557,"version":"4.1.0"},"reference-count":46,"publisher":"Korean Society of Animal Science and Technology","issue":"4","license":[{"start":{"date-parts":[[2023,7,1]],"date-time":"2023-07-01T00:00:00Z","timestamp":1688169600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0"}],"content-domain":{"domain":["www.ejast.org"],"crossmark-restriction":true},"short-container-title":["J Anim Sci Technol"],"published-print":{"date-parts":[[2023,7]]},"abstract":"<jats:p>This study was conducted to evaluate the effects of co-dried fish protein\n                    hydrolysate (CFPH) on broilers performance, intestinal microbiology, and\n                    cellular immune responses. Five hundred one-day-old (Ross 308) male broilers\n                    were allocated to four treatments with five replicates of 25 birds in a\n                    completely randomized design. The experimental treatments included four levels\n                    of CFPH (0% as the control, 2.5%, 5%, and 7.5%) in the isonitrogenous and\n                    isocaloric diets. During the experiment, body weight (BW) and feed intake (FI)\n                    were periodically recorded in addition to calculating average daily gain (ADG),\n                    feed conversion ratio (FCR), liveability index, and European broiler index\n                    (EBI). In addition, cellular immune responses were evaluated at 30 days of age.\n                    On day 42, ileal contents were obtained to examine the microbial population.\n                    Based on the findings, Dietary supplementation of 5 and 7.5% CFPH increased the\n                    percentage of the thigh while decreasing the relative weight of the gizzard\n                    compared to the control group. The highest relative length of jejunum was\n                    observed in birds receiving 2.5 and 5% CFPH, and its highest relative weight\n                    belonged to birds fed with 5% CFPH. The number of coliforms, enterobacters, and\n                    total gram-negative bacteria in the intestines of birds receiving CFPH was less\n                    than that of the control group. In general, the application of CFPH in broiler\n                    nutrition can decrease the level of soybean meal in diet and it can be\n                    considered as a new protein supplement in poultry production. It is suggested to\n                    study the incorporation of this new supplement in other livestock\u2019s\n                    diets.<\/jats:p>","DOI":"10.5187\/jast.2022.e99","type":"journal-article","created":{"date-parts":[[2022,12,20]],"date-time":"2022-12-20T04:09:57Z","timestamp":1671509397000},"page":"804-817","update-policy":"https:\/\/doi.org\/10.5187\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Effects of a new generation of fish protein hydrolysate on\n                    performance, intestinal microbiology, and immunity of broiler\n                    chickens"],"prefix":"10.5187","volume":"65","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5654-0979","authenticated-orcid":false,"given":"Amir Hossein","family":"Alizadeh-Ghamsari","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/032hv6w38","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Animal and Poultry Nutrition and Physiology, Animal Science Research Institute of Iran, Agricultural Research, Education and Extension Organization (AREEO), Karaj 3146618361, Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5826-4231","authenticated-orcid":false,"given":"Amir Reza","family":"Shaviklo","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/032hv6w38","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Animal Processing, Animal Science Research Institute of Iran, Agricultural Research, Education and Extension Organization (AREEO), Karaj 3146618361, Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1762-252X","authenticated-orcid":false,"given":"Seyyed Abdullah","family":"Hosseini","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/032hv6w38","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Animal and Poultry Nutrition and Physiology, Animal Science Research Institute of Iran, Agricultural Research, Education and Extension Organization (AREEO), Karaj 3146618361, Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"3132","published-online":{"date-parts":[[2023,7,30]]},"reference":[{"key":"key2.0261002112817e+13_B1","doi-asserted-by":"crossref","first-page":"4097","DOI":"10.1002\/jsfa.8926","article-title":"Preparation of fish waste silage and its effect on the growth\n                        performance and meat quality of broiler chickens","volume":"98","author":"Shabani","year":"2018","journal-title":"J Sci Food Agric."},{"key":"key2.0261002112817e+13_B2","doi-asserted-by":"crossref","first-page":"123219","DOI":"10.1016\/j.jclepro.2020.123219","article-title":"Development of animal\/ plant-based protein hydrolysate and its\n                        application in food, feed and nutraceutical industries: state of the\n                        art","volume":"278","author":"Etemadian","year":"2021","journal-title":"J Clean Prod."},{"key":"key2.0261002112817e+13_B3","first-page":"187","volume-title":"Advances in food and nutrition research.","author":"Shavandi","year":"2019","edition":"editor"},{"key":"key2.0261002112817e+13_B4","doi-asserted-by":"crossref","first-page":"4775","DOI":"10.1007\/s13197-019-03966-5","article-title":"Overcoming current challenges in commercial applications of fish\n                        protein isolates in food and feed systems: a review","volume":"56","author":"Shaviklo","year":"2019","journal-title":"J Food Sci Technol."},{"key":"key2.0261002112817e+13_B5","volume-title":"Fennema\u2019s food chemistry.","author":"Damodaran","year":"2017","edition":"5th ed."},{"key":"key2.0261002112817e+13_B6","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1080\/10408690091189266","article-title":"Fish protein hydrolysates: production, biochemical, and\n                        functional properties","volume":"40","author":"Kristinsson","year":"2000","journal-title":"Crit Rev Food Sci Nutr."},{"key":"key2.0261002112817e+13_B7","doi-asserted-by":"crossref","first-page":"223","DOI":"10.1007\/s40071-018-0207-4","article-title":"Production of fish protein hydrolysates step by step:\n                        technological aspects, equipment used, major energy costs and methods of\n                        their minimizing","volume":"10","author":"Petrova","year":"2018","journal-title":"Int Aquat Res."},{"key":"key2.0261002112817e+13_B8","doi-asserted-by":"crossref","first-page":"167","DOI":"10.1016\/0377-8401(94)90024-8","article-title":"Chemical and nutritional quality of dried fermented fish silages\n                        and their nutritive value for tilapia (Oreochromis\n                        niloticus)","volume":"45","author":"Fagbenro","year":"1994","journal-title":"Anim Feed Sci Technol."},{"key":"key2.0261002112817e+13_B9","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1300\/J030v10n04_04","article-title":"Chemical and nutritional characteristics 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test","volume":"69","author":"Corrier","year":"1990","journal-title":"Poult Sci."},{"key":"key2.0261002112817e+13_B24","doi-asserted-by":"crossref","first-page":"512","DOI":"10.1080\/00071660412331286226","article-title":"Effect of graded levels of aflatoxin, ochratoxin and their\n                        combinations on the performance and immune response of\n                        broilers","volume":"45","author":"Verma","year":"2004","journal-title":"Br Poult Sci."},{"key":"key2.0261002112817e+13_B25","doi-asserted-by":"crossref","first-page":"2500","DOI":"10.3382\/ps.2011-01393","article-title":"Black cumin seeds, Artemisia leaves (Artemisia sieberi), and\n                        Camellia L. plant extract as phytogenic products in broiler diets and their\n                        effects on performance, blood constituents, immunity, and cecal microbial\n                        population","volume":"90","author":"Khalaji","year":"2011","journal-title":"Poult Sci."},{"key":"key2.0261002112817e+13_B26","doi-asserted-by":"crossref","first-page":"51","DOI":"10.4081\/ijas.2009.51","article-title":"Evaluation of intestinal bacterial flora of conventional and\n                        organic broilers using culture-based methods","volume":"8","author":"Proietti","year":"2009","journal-title":"Ital J Anim Sci."},{"key":"key2.0261002112817e+13_B27","doi-asserted-by":"crossref","first-page":"925","DOI":"10.1093\/ps\/83.6.925","article-title":"Influence of whole wheat and xylanase on broiler performance and\n                        microbial composition and activity in the digestive tract","volume":"83","author":"Engberg","year":"2004","journal-title":"Poult Sci."},{"key":"key2.0261002112817e+13_B28","doi-asserted-by":"crossref","first-page":"5330","DOI":"10.3382\/ps\/pez321","article-title":"Development of a novel in ovo challenge model for virulent\n                        Escherichia coli strains","volume":"98","author":"Graham","year":"2019","journal-title":"Poult Sci."},{"key":"key2.0261002112817e+13_B29","volume-title":"SAS\/STAT\u00ae 9.1 user\u2019s guide.","author":"SAS","year":"2004"},{"key":"key2.0261002112817e+13_B30","doi-asserted-by":"crossref","first-page":"1","DOI":"10.2307\/3001478","article-title":"Multiple range and multiple F tests","volume":"11","author":"Duncan","year":"1955","journal-title":"Biometrics."},{"key":"key2.0261002112817e+13_B31","doi-asserted-by":"crossref","first-page":"1614","DOI":"10.5713\/ajas.2010.10119","article-title":"The effect of feeding different levels of sardine fish silage on\n                        broiler performance, meat quality and sensory characteristics under closed\n                        and open-sided housing systems","volume":"23","author":"Al-Marzooqi","year":"2010","journal-title":"Asian-Australas J Anim Sci."},{"key":"key2.0261002112817e+13_B32","doi-asserted-by":"crossref","first-page":"4293","DOI":"10.35759\/JAnmPlSci.v27-3.4","article-title":"Use of biological fish silage in broilers feed: effect on growth\n                        performance and meat quality","volume":"27","author":"Ram\u00edrez","year":"2016","journal-title":"J Anim Plant Sci."},{"key":"key2.0261002112817e+13_B33","doi-asserted-by":"crossref","first-page":"1002","DOI":"10.1590\/S1516-89132013000600016","article-title":"Preparation of biological fish silage and its effect on the\n                        performance and meat quality characteristics of quails (Coturnix coturnix\n                        japonica)","volume":"56","author":"Ram\u00edrez","year":"2013","journal-title":"Braz Arch Biol Technol."},{"key":"key2.0261002112817e+13_B34","doi-asserted-by":"crossref","first-page":"1569","DOI":"10.3382\/ps\/pev104","article-title":"Early intestinal growth and development in\n                        poultry","volume":"94","author":"Lilburn","year":"2015","journal-title":"Poult Sci."},{"key":"key2.0261002112817e+13_B35","doi-asserted-by":"crossref","first-page":"2795","DOI":"10.3390\/ani11102795","article-title":"Nutrition and digestive physiology of the broiler chick: state of\n                        the art and outlook","volume":"11","author":"Ravindran","year":"2021","journal-title":"Animals."},{"key":"key2.0261002112817e+13_B36","first-page":"1","article-title":"Effects of dietary fish oil and green tea powder supplementation\n                        on broiler chickens immunity","volume":"42","author":"Seidavi","year":"2014","journal-title":"Acta Sci Vet."},{"key":"key2.0261002112817e+13_B37","doi-asserted-by":"crossref","first-page":"4832","DOI":"10.1016\/j.psj.2020.06.073","article-title":"Effects of different rearing systems on intramuscular fat\n                        content, fatty acid composition, and lipid metabolism-related genes\n                        expression in breast and thigh muscles of Nonghua ducks","volume":"99","author":"Guo","year":"2020","journal-title":"Poult Sci."},{"key":"key2.0261002112817e+13_B38","article-title":"Effect of fish offal meal on feed intake, growth performances and\n                        carcass characteristics of Hubbard Classic broiler chicken\n                        breeds","volume":"371","author":"Kiflay","year":"2018","journal-title":"J Agric Environ Sci."},{"key":"key2.0261002112817e+13_B39","first-page":"2949","article-title":"Gizzerosine, a new toxic substance in fish meal, causes severe\n                        gizzard erosion in chicks","volume":"47","author":"Okazaki","year":"1983","journal-title":"Agric Biol Chem."},{"key":"key2.0261002112817e+13_B40","first-page":"1","article-title":"Causes of gizzard erosion and proventriculitis in\n                        broilers","author":"Contreras","year":"2007","journal-title":"Poult Inf Prof."},{"key":"key2.0261002112817e+13_B41","doi-asserted-by":"crossref","first-page":"114874","DOI":"10.1016\/j.anifeedsci.2021.114874","article-title":"Evaluation of increasing concentrations of fish waste silage in\n                        diets on growth performance, gastrointestinal microbial population, and\n                        intestinal morphology of broiler chickens","volume":"275","author":"Shabani","year":"2021","journal-title":"Anim Feed Sci Technol."},{"key":"key2.0261002112817e+13_B42","first-page":"105","article-title":"Intestinal characteristics, alkaline phosphatase and broilers\n                        performance in response to extracted and mechanical soybean meal replaced by\n                        fish meal","volume":"14","author":"Saki","year":"2012","journal-title":"J Agric Sci Technol."},{"key":"key2.0261002112817e+13_B43","doi-asserted-by":"crossref","first-page":"178","DOI":"10.1016\/j.wasman.2017.04.017","article-title":"Study on the chemical and microbial composition and probiotic\n                        characteristics of dominant lactic acid bacteria in fermented poultry\n                        slaughterhouse waste","volume":"65","author":"Ashayerizadeh","year":"2017","journal-title":"Waste Manag."},{"key":"key2.0261002112817e+13_B44","doi-asserted-by":"crossref","first-page":"1735","DOI":"10.3389\/fimmu.2020.01735","article-title":"Dietary supplementation with various fat oils affect\n                        phytohemagglutinin skin test in broiler chickens","volume":"11","author":"Al-Khalaifah","year":"2020","journal-title":"Front Immunol."},{"key":"key2.0261002112817e+13_B45","doi-asserted-by":"crossref","first-page":"397","DOI":"10.21071\/az.v64i248.426","article-title":"Evaluation of dietary fish oil plus green tea supplementation on\n                        the gizzard, ileum and cecum microflora in broiler chickens","volume":"64","author":"Seidavi","year":"2015","journal-title":"Arch Zootec."},{"key":"key2.0261002112817e+13_B46","doi-asserted-by":"crossref","first-page":"284","DOI":"10.3382\/japr\/pfv077","article-title":"The use and abuse of immune indices in nutritional immunology\n                        studies","volume":"25","author":"Selvaraj","year":"2016","journal-title":"J Appl Poult Res."}],"container-title":["Journal of Animal Science and Technology"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/www.ejast.org\/download\/download_pdf?doi=10.5187\/jast.2022.e99","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/www.ejast.org\/download\/download_pdf?doi=10.5187\/jast.2022.e99","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T02:53:46Z","timestamp":1790909626000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.ejast.org\/archive\/view_article?doi=10.5187\/jast.2022.e99"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,7]]},"references-count":46,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2023,7,30]]},"published-print":{"date-parts":[[2023,7]]}},"alternative-id":["10.5187\/jast.2022.e99"],"URL":"https:\/\/doi.org\/10.5187\/jast.2022.e99","relation":{},"ISSN":["2672-0191","2055-0391"],"issn-type":[{"value":"2672-0191","type":"print"},{"value":"2055-0391","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,7]]},"assertion":[{"value":"2022-08-13","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication 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