{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T21:17:40Z","timestamp":1792790260942,"version":"4.5.0"},"reference-count":26,"publisher":"Korean Society of Animal Science and Technology","issue":"6","license":[{"start":{"date-parts":[[2023,11,1]],"date-time":"2023-11-01T00:00:00Z","timestamp":1698796800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0"}],"funder":[{"name":"Daegu Catholic University","id":[{"id":"https:\/\/ror.org\/04fxknd68","id-type":"ROR","asserted-by":"crossref"}]}],"content-domain":{"domain":["www.ejast.org"],"crossmark-restriction":true},"short-container-title":["J Anim Sci Technol"],"published-print":{"date-parts":[[2023,11]]},"abstract":"<jats:p>The goals of the present study were to develop a simple method for obtain highly\n                    purified pig sperm hyaluronidase (pHyase) and to assess its activity, function,\n                    and safety. In mammals, sperm-specific glycophosphatidylinositol (GPI)-anchored\n                    Hyase assists sperm penetration through the cumulus mass surrounding the egg and\n                    aids in the dispersal of the cumulus\u2013oocyte complex. Recently, Purified\n                    bovine sperm hyaluronidase (bHyase) has been shown to enhance therapeutic drug\n                    transport by breaking down the hyaluronan barrier to the lymphatic and capillary\n                    vessels, thereby facilitating tissue absorption. Commercially available Hyase is\n                    typically isolated from bovine or ovine; which have several disadvantages,\n                    including the risk of bovine spongiform encephalopathy, low homology with human\n                    Hyase, and the requirement for relatively complex isolation procedures. This\n                    study successfully isolated highly purified pHyase in only two steps, using\n                    ammonium sulfate precipitation and fast protein liquid chromatography. The\n                    isolated Hyase had activity equal to that of commercial bHyase, facilitated in\n                    vitro fertilization, and effectively dissolved high molecule hyaluronic acid.\n                    This simple, effective isolation method could improve the availability of pHyase\n                    for research and clinical applications.<\/jats:p>","DOI":"10.5187\/jast.2023.e53","type":"journal-article","created":{"date-parts":[[2023,6,9]],"date-time":"2023-06-09T05:13:11Z","timestamp":1686287591000},"page":"1205-1213","update-policy":"https:\/\/doi.org\/10.5187\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Industrialization possibilities of purified pig sperm\n                    hyaluronidase"],"prefix":"10.5187","volume":"65","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9901-719X","authenticated-orcid":false,"given":"Soojin","family":"Park","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/04fxknd68","id-type":"ROR","asserted-by":"publisher"}],"name":"College of Pharmacy, Catholic University of Daegu, Gyeongsan 38430, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0085-2994","authenticated-orcid":false,"given":"In-Soo","family":"Myeong","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/04fxknd68","id-type":"ROR","asserted-by":"publisher"}],"name":"College of Pharmacy, Catholic University of Daegu, Gyeongsan 38430, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7917-3726","authenticated-orcid":false,"given":"Gabbine","family":"Wee","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/05cc1v231","id-type":"ROR","asserted-by":"publisher"}],"name":"Laboratory Animal Center, Daegu-Gyeongbuk Medical Innovation Foundation (DGMIF), Daegu 41061, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3088-8517","authenticated-orcid":false,"given":"Ekyune","family":"Kim","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/04fxknd68","id-type":"ROR","asserted-by":"publisher"}],"name":"College of Pharmacy, Catholic University of Daegu, Gyeongsan 38430, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"3132","published-online":{"date-parts":[[2023,11,30]]},"reference":[{"key":"key2.0261002112924e+13_B1","doi-asserted-by":"crossref","first-page":"283","DOI":"10.3390\/ijms19010283","article-title":"The biological role of hyaluronan-rich oocyte-cumulus\n                        extracellular matrix in female reproduction","volume":"19","author":"Nagyova","year":"2018","journal-title":"Int J Mol Sci."},{"key":"key2.0261002112924e+13_B2","doi-asserted-by":"crossref","first-page":"4","DOI":"10.1016\/j.addr.2015.11.001","article-title":"Extracellular matrix structure","volume":"97","author":"Theocharis","year":"2016","journal-title":"Adv Drug Deliv Rev."},{"key":"key2.0261002112924e+13_B3","doi-asserted-by":"crossref","first-page":"471","DOI":"10.1007\/s11892-012-0297-0","article-title":"The role of hyaluronan and the extracellular matrix in islet\n                        inflammation and immune regulation","volume":"12","author":"Bollyky","year":"2012","journal-title":"Curr Diab Rep."},{"key":"key2.0261002112924e+13_B4","doi-asserted-by":"crossref","first-page":"130","DOI":"10.1016\/j.actbio.2017.01.064","article-title":"Hyaluronic acid facilitates chondrogenesis and matrix deposition\n                        of human adipose derived mesenchymal stem cells and human chondrocytes\n                        co-cultures","volume":"52","author":"Amann","year":"2017","journal-title":"Acta Biomater."},{"key":"key2.0261002112924e+13_B5","doi-asserted-by":"crossref","first-page":"939","DOI":"10.1095\/biolreprod.109.078816","article-title":"Functional roles of mouse sperm hyaluronidases, HYAL5 and SPAM1,\n                        in fertilization","volume":"81","author":"Kimura","year":"2009","journal-title":"Biol Reprod."},{"key":"key2.0261002112924e+13_B6","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/S0006-291X(03)00052-4","article-title":"Hypothesis: a glycoprotein-degradation complex formed by\n                        protein\u2013protein interaction involves cytoplasmic\n                        peptide:N-glycanase","volume":"302","author":"Suzuki","year":"2003","journal-title":"Biochem Biophys Res Commun."},{"key":"key2.0261002112924e+13_B7","doi-asserted-by":"crossref","first-page":"635","DOI":"10.1096\/fj.01-0889rev","article-title":"Cytoplasmic peptide: N\u2010glycanase (PNGase) in eukaryotic\n                        cells: occurrence, primary structure, and potential\n                        functions","volume":"16","author":"Suzuki","year":"2002","journal-title":"FASEB J."},{"key":"key2.0261002112924e+13_B8","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1095\/biolreprod.113.115857","article-title":"Hyaluronidase 2: a novel germ cell hyaluronidase with epididymal\n                        expression and functional roles in mammalian sperm","volume":"91","author":"Modelski","year":"2014","journal-title":"Biol Reprod."},{"key":"key2.0261002112924e+13_B9","doi-asserted-by":"crossref","first-page":"762","DOI":"10.1016\/j.semcdb.2007.09.010","article-title":"Cytoplasmic peptide:N-glycanase and catabolic pathway for free\n                        N-glycans in the cytosol","volume":"18","author":"Suzuki","year":"2007","journal-title":"Semin Cell Dev Biol."},{"key":"key2.0261002112924e+13_B10","doi-asserted-by":"crossref","first-page":"94","DOI":"10.1007\/s003359900365","article-title":"The mouse Spam1 maps to proximal chromosome 6 and is a candidate\n                        for the sperm dysfunction in Rb(6.16)24Lub and Rb(6.15)lAld\n                        heterozygotes","volume":"8","author":"Deng","year":"1997","journal-title":"Mamm Genome."},{"key":"key2.0261002112924e+13_B11","doi-asserted-by":"crossref","first-page":"499","DOI":"10.1016\/S0945-053X(01)00172-X","article-title":"The six hyaluronidase-like genes in the human and mouse\n                        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male\n                        subfertility caused by a cumulus-oocyte complex penetration\n                        defect","volume":"33","author":"Park","year":"2019","journal-title":"FASEB J."},{"key":"key2.0261002112924e+13_B21","doi-asserted-by":"crossref","first-page":"783","DOI":"10.1016\/j.bbrc.2006.09.133","article-title":"Hyaluronidase increases the biodistribution of acid \u03b1-1,4\n                        glucosidase in the muscle of Pompe disease mice: an approach to enhance the\n                        efficacy of enzyme replacement therapy","volume":"350","author":"Matalon","year":"2006","journal-title":"Biochem Biophys Res Commun."},{"key":"key2.0261002112924e+13_B22","doi-asserted-by":"crossref","first-page":"1072","DOI":"10.1095\/biolreprod50.5.1072","article-title":"In vitro development of in vitro-matured porcine oocytes\n                        following chemical activation or in vitro 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with subcutaneous injection and\n                        continuous infusion","volume":"17","author":"Vaughn","year":"2011","journal-title":"Endocr Pract."},{"key":"key2.0261002112924e+13_B26","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1016\/j.anireprosci.2014.09.002","article-title":"Characterization of pig sperm hyaluronidase and improvement of\n                        the digestibility of cumulus cell mass by recombinant pSPAM1 hyaluronidase\n                        in an in vitro fertilization assay","volume":"150","author":"Yoon","year":"2014","journal-title":"Anim Reprod Sci."}],"container-title":["Journal of Animal Science and 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