{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T02:56:00Z","timestamp":1790909760723,"version":"4.1.0"},"reference-count":36,"publisher":"Korean Society of Animal Science and Technology","issue":"2","license":[{"start":{"date-parts":[[2024,3,1]],"date-time":"2024-03-01T00:00:00Z","timestamp":1709251200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0"}],"funder":[{"name":"National Research Foundation of Korea","award":["NRF-2021R1A2C3011051"],"award-info":[{"award-number":["NRF-2021R1A2C3011051"]}],"id":[{"id":"https:\/\/ror.org\/013aysd81","id-type":"ROR","asserted-by":"crossref"}]}],"content-domain":{"domain":["www.ejast.org"],"crossmark-restriction":true},"short-container-title":["J Anim Sci Technol"],"published-print":{"date-parts":[[2024,3]]},"abstract":"<jats:p>Upregulation of the nutritional value of feed is the major target of various\n                    studies in the livestock industry, and dietary enzyme supplementation could aid\n                    in digesting the nondegrading nutrients of grains in feed ingredients. Dried\n                    distillers\u2019 grains with solubles (DDGS) is a byproduct of the\n                    fermentation process in the beverage industry and can be used as a large supply\n                    source of fiber in feed. Therefore, we conducted an experiment with male broiler\n                    chickens to investigate the effect of various types of enzymes on DDGS and\n                    compare the efficacy of single enzyme and multienzyme complexes on growth\n                    performance and gut environments in broiler chickens. We used 420 1-day-old\n                    broiler chickens (Ross 308), and they were allotted into 4 dietary treatments\n                    with seven replications (CON, corn-soybean meal [SBM] diet; NC, DDGS\n                    supplemented diet; SE, 0.05 % of mannanase supplemented DDGS-based diet; MC,\n                    0.10% of multienzyme complex (mannanase and xylanase, glucanase) supplemented\n                    DDGS-based diet. The dietary exogenous enzyme in the DDGS-supplemented diet\n                    could improve growth performance as much as the growth of the control group, and\n                    digestibility of dry matter, crude protein, and gross energy were significantly\n                    increased by enzyme addition in groups of chicks fed DDGS-supplementation diet.\n                    Moreover, the populations of pathogenic bacteria, coliforms, and\n                        Bacteroidetes were significantly decreased by enzyme\n                    supplementation, which might lead to improved gut mucus-secreting cells and\n                    inflammatory cytokines in the jejunum. Collectively, dietary single enzyme and\n                    multienzyme complexes could improve gut environments, including intestinal\n                    immune responses and gut microbial population, and lead to improvement of growth\n                    performance in broiler chickens.<\/jats:p>","DOI":"10.5187\/jast.2023.e90","type":"journal-article","created":{"date-parts":[[2023,9,12]],"date-time":"2023-09-12T20:38:02Z","timestamp":1694551082000},"page":"398-411","update-policy":"https:\/\/doi.org\/10.5187\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Comparison study between single enzyme and multienzyme complex in\n                    distiller\u2019s dred grains with soluble supplemented diet in broiler\n                    chicken"],"prefix":"10.5187","volume":"66","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9832-3251","authenticated-orcid":false,"given":"Min-Jin","family":"Kwak","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/04h9pn542","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Agricultural Biotechnology and Research Institute of Agriculture and Life Science, Seoul National University, Seoul 08826, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3997-1600","authenticated-orcid":false,"given":"Dong-Jin","family":"Ha","sequence":"additional","affiliation":[{"name":"Division of Biotechnology, College of Life Science and Biotechnology, Korea University, Seoul 02841, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1438-9515","authenticated-orcid":false,"given":"Min Young","family":"Park","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/0190ak572","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Basic Science and Craniofacial Biology, New York University College of Dentistry, New York 10012, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3764-3339","authenticated-orcid":false,"given":"Ju Young","family":"Eor","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/04h9pn542","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Agricultural Biotechnology and Research Institute of Agriculture and Life Science, Seoul National University, Seoul 08826, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0414-7611","authenticated-orcid":false,"given":"Kwang-Youn","family":"Whang","sequence":"additional","affiliation":[{"name":"Division of Biotechnology, College of Life Science and Biotechnology, Korea University, Seoul 02841, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6769-0657","authenticated-orcid":false,"given":"Younghoon","family":"Kim","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/04h9pn542","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Agricultural Biotechnology and Research Institute of Agriculture and Life Science, Seoul National University, Seoul 08826, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"3132","published-online":{"date-parts":[[2024,3,31]]},"reference":[{"key":"key2.0261002113056e+13_B1","doi-asserted-by":"crossref","first-page":"1275","DOI":"10.5187\/jast.2021.e111","article-title":"Effects of dietary palm kernel meal and \u03b2-xylanase on\n                        productive performance, fatty liver incidence, and excreta characteristics\n                        in laying hens","volume":"63","author":"Choi","year":"2021","journal-title":"J Anim Sci Technol."},{"key":"key2.0261002113056e+13_B2","doi-asserted-by":"crossref","first-page":"462","DOI":"10.5187\/jast.2022.e28","article-title":"Effects of dietary protease supplementation on growth rate,\n                        nutrient digestibility, and intestinal morphology of weaned\n                        pigs","volume":"64","author":"Song","year":"2022","journal-title":"J Anim Sci Technol."},{"key":"key2.0261002113056e+13_B3","doi-asserted-by":"crossref","first-page":"409","DOI":"10.5187\/jast.2022.e27","article-title":"How to develop strategies to use insects as animal feed:\n                        digestibility, functionality, safety, and regulation","volume":"64","author":"Lee","year":"2022","journal-title":"J Anim Sci Technol."},{"key":"key2.0261002113056e+13_B4","doi-asserted-by":"crossref","first-page":"114833","DOI":"10.1016\/j.anifeedsci.2021.114833","article-title":"The effects of multispecies probiotic formulations on growth\n                        performance, hepatic metabolism, intestinal integrity and fecal microbiota\n                        in growing-finishing pigs","volume":"274","author":"Kwak","year":"2021","journal-title":"Anim Feed Sci Technol."},{"key":"key2.0261002113056e+13_B5","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1186\/s40104-021-00606-x","article-title":"Dietary sophorolipid accelerates growth by modulation of gut\n                        microbiota population and intestinal environments in broiler\n                        chickens","volume":"12","author":"Kwak","year":"2021","journal-title":"J Anim Sci Biotechnol."},{"key":"key2.0261002113056e+13_B6","doi-asserted-by":"crossref","first-page":"520","DOI":"10.5187\/jast.2021.e44","article-title":"Effects of dietary inactivated probiotics on growth performance\n                        and immune responses of weaned pigs","volume":"63","author":"Kang","year":"2021","journal-title":"J Anim Sci Technol."},{"key":"key2.0261002113056e+13_B7","doi-asserted-by":"crossref","first-page":"5","DOI":"10.1079\/WPS200480","article-title":"Enzymes for the feed industry: past, present and\n                        future","volume":"62","author":"Choct","year":"2006","journal-title":"Worlds Poult Sci J."},{"key":"key2.0261002113056e+13_B8","doi-asserted-by":"crossref","first-page":"491","DOI":"10.5187\/jast.2021.e49","article-title":"Effect of low protein diets added with protease on growth\n                        performance, nutrient digestibility of weaned piglets and growing-finishing\n                        pigs","volume":"63","author":"Kim","year":"2021","journal-title":"J Anim Sci Technol."},{"key":"key2.0261002113056e+13_B9","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1016\/j.anifeedsci.2014.05.011","article-title":"Use of distiller\u2019s dried grains with solubles (DDGS) in\n                        rainbow trout feeds","volume":"195","author":"Welker","year":"2014","journal-title":"Anim Feed Sci Technol."},{"key":"key2.0261002113056e+13_B10","doi-asserted-by":"crossref","first-page":"990","DOI":"10.3923\/ijps.2008.990.996","article-title":"Evaluation of high levels of distillers dried grains with\n                        solubles (DDGS) in broiler diets","volume":"7","author":"Wang","year":"2008","journal-title":"Int J Poult Sci."},{"key":"key2.0261002113056e+13_B11","doi-asserted-by":"crossref","first-page":"7702","DOI":"10.1021\/jf301674u","article-title":"Solid-state fermentation of soybean and corn processing\n                        coproducts for potential feed improvement","volume":"60","author":"Lio","year":"2012","journal-title":"J Agric Food Chem."},{"key":"key2.0261002113056e+13_B12","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/S0377-8401(00)00155-3","article-title":"Exogenous enzymes in monogastric nutrition \u2014 their current\n                        value and future benefits","volume":"86","author":"Bedford","year":"2000","journal-title":"Anim Feed Sci Technol."},{"key":"key2.0261002113056e+13_B13","doi-asserted-by":"crossref","first-page":"400","DOI":"10.5713\/ajas.2011.10273","article-title":"Effect of exogenous xylanase supplementation on the performance,\n                        net energy and gut microflora of broiler chickens fed wheat-based\n                        diets","volume":"24","author":"Nian","year":"2011","journal-title":"Asian-Australas J Anim Sci."},{"key":"key2.0261002113056e+13_B14","doi-asserted-by":"crossref","first-page":"91","DOI":"10.4025\/actascianimsci.v39i1.32765","article-title":"Performance and economic viability of broiler chicken fed diets\n                        with multienzyme complexes","volume":"39","author":"Pasquali","year":"2017","journal-title":"Acta Sci Anim Sci."},{"key":"key2.0261002113056e+13_B15","doi-asserted-by":"crossref","first-page":"1301","DOI":"10.5187\/jast.2021.e107","article-title":"Effects of applying cellulase and starch on the fermentation\n                        characteristics and microbial communities of Napier grass (Pennisetum\n                        purpureum Schum.) silage","volume":"63","author":"Zhao","year":"2021","journal-title":"J Anim Sci Technol."},{"key":"key2.0261002113056e+13_B16","doi-asserted-by":"crossref","first-page":"380","DOI":"10.3390\/ani12030380","article-title":"Effects of dietary multienzyme complex supplementation on growth\n                        performance, digestive capacity, histomorphology, blood metabolites and\n                        hepatic glycometabolism in snakehead (Channa argus)","volume":"12","author":"Ding","year":"2022","journal-title":"Animals."},{"key":"key2.0261002113056e+13_B17","doi-asserted-by":"crossref","first-page":"1328","DOI":"10.5187\/jast.2021.e104","article-title":"Multi-carbohydrase application into energy and amino acid\n                        deficient broiler diets: a strategy to enhance performance of broiler\n                        chickens","volume":"63","author":"Wickramasuriya","year":"2021","journal-title":"J Anim Sci Technol."},{"key":"key2.0261002113056e+13_B18","doi-asserted-by":"crossref","first-page":"1092","DOI":"10.5187\/jast.2022.e76","article-title":"Dietary effects of sophorolipids on nutrient bioavailability and\n                        intestinal microenvironments in broiler chickens","volume":"64","author":"Kwak","year":"2022","journal-title":"J Anim Sci Technol."},{"key":"key2.0261002113056e+13_B19","doi-asserted-by":"crossref","first-page":"104","DOI":"10.5187\/jast.2021.e16","article-title":"Effects of microencapsulated organic acids on growth performance,\n                        nutrient digestibility, fecal microbial counts, and blood profiles in\n                        weaning pigs","volume":"63","author":"Lee","year":"2021","journal-title":"J Anim Sci Technol."},{"key":"key2.0261002113056e+13_B20","doi-asserted-by":"crossref","first-page":"100193","DOI":"10.1016\/j.japr.2021.100193","article-title":"Xylanase impact beyond performance: a prebiotic approach in\n                        broiler chickens","volume":"30","author":"Van Hoeck","year":"2021","journal-title":"J Appl Poult Res."},{"key":"key2.0261002113056e+13_B21","doi-asserted-by":"crossref","DOI":"10.1093\/tas\/txab160","article-title":"Significance of single \u03b2-mannanase supplementation on\n                        performance and energy utilization in broiler chickens, laying hens,\n                        turkeys, sows, and nursery-finish pigs: a meta-analysis and systematic\n                        review","volume":"5","author":"Kiarie","year":"2021","journal-title":"Transl Anim Sci."},{"key":"key2.0261002113056e+13_B22","doi-asserted-by":"crossref","first-page":"749","DOI":"10.1080\/00071668.2019.1652884","article-title":"Evaluation of a high-protein DDGS product in broiler chickens:\n                        performance, nitrogen-corrected apparent metabolisable energy, and\n                        standardised ileal amino acid digestibility","volume":"60","author":"Fries-Craft","year":"2019","journal-title":"Br Poult Sci."},{"key":"key2.0261002113056e+13_B23","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1590\/1806-9061-2019-1088","article-title":"Effect of exogenous protease, mannanase, and xylanase\n                        supplementation in corn and high protein corn DDGS based diets on growth\n                        performance, intestinal morphology and nutrient digestibility in broiler\n                        chickens","volume":"21","author":"Hussain","year":"2019","journal-title":"Braz J Poult Sci."},{"key":"key2.0261002113056e+13_B24","doi-asserted-by":"crossref","first-page":"101312","DOI":"10.1016\/j.psj.2021.101312","article-title":"Effects of dietary protein, energy and \u03b2-mannanase on\n                        laying performance, egg quality, and ileal amino acid digestibility in\n                        laying hens","volume":"100","author":"White","year":"2021","journal-title":"Poult Sci."},{"key":"key2.0261002113056e+13_B25","doi-asserted-by":"crossref","first-page":"40","DOI":"10.3382\/japr\/pfv059","article-title":"Effect of \u03b2-mannanase inclusion on growth performance,\n                        ileal digestible energy, and intestinal viscosity of male broilers fed a\n                        reduced-energy diet","volume":"25","author":"Latham","year":"2016","journal-title":"J Appl Poult Res."},{"key":"key2.0261002113056e+13_B26","doi-asserted-by":"crossref","first-page":"69","DOI":"10.5187\/jast.2021.e1","article-title":"Supplementation of enzyme cocktail in chickens diet is an\n                        effective approach to increase the utilization of nutrient in wheat-based\n                        diets","volume":"63","author":"Ko","year":"2021","journal-title":"J Anim Sci Technol."},{"key":"key2.0261002113056e+13_B27","doi-asserted-by":"crossref","first-page":"1579","DOI":"10.3382\/ps.2012-02786","article-title":"Evaluation of high dietary inclusion of distillers dried grains\n                        with solubles and supplementation of protease and xylanase in the diets of\n                        broiler chickens under necrotic enteritis challenge","volume":"92","author":"Barekatain","year":"2013","journal-title":"Poult Sci."},{"key":"key2.0261002113056e+13_B28","doi-asserted-by":"crossref","DOI":"10.1111\/j.1439-0396.2009.00973.x","article-title":"Effects of dietary supplementation of synbiotics and phytobiotics\n                        on performance, caecal coliform population and some oxidant\/antioxidant\n                        parameters of broilers","volume":"94","author":"Erdo\u011fan","year":"2010","journal-title":"J Anim Physiol Anim Nutr."},{"key":"key2.0261002113056e+13_B29","doi-asserted-by":"crossref","first-page":"309","DOI":"10.1093\/ps\/86.2.309","article-title":"Evaluation of the efficacy of a probiotic containing\n                        Lactobacillus, Bifidobacterium, Enterococcus, and Pediococcus strains in\n                        promoting broiler performance and modulating cecal microflora composition\n                        and metabolic activities","volume":"86","author":"Mountzouris","year":"2007","journal-title":"Poult Sci."},{"key":"key2.0261002113056e+13_B30","doi-asserted-by":"crossref","first-page":"1184","DOI":"10.5187\/jast.2022.e89","article-title":"Intestinal microbial composition changes induced by Lactobacillus\n                        plantarum GBL 16, 17 fermented feed and intestinal immune homeostasis\n                        regulation in pigs","volume":"64","author":"Yu","year":"2022","journal-title":"J Anim Sci Technol."},{"key":"key2.0261002113056e+13_B31","doi-asserted-by":"crossref","first-page":"507","DOI":"10.3382\/ps\/pev362","article-title":"Effect of yeast-derived products and distillers dried grains with\n                        solubles (DDGS) on growth performance, gut morphology, and gene expression\n                        of pattern recognition receptors and cytokines in broiler\n                        chickens","volume":"95","author":"Alizadeh","year":"2016","journal-title":"Poult Sci."},{"key":"key2.0261002113056e+13_B32","doi-asserted-by":"crossref","first-page":"283","DOI":"10.1186\/s12917-021-02946-2","article-title":"Effects of different feeding regimens with protease\n                        supplementation on growth, amino acid digestibility, economic efficiency,\n                        blood biochemical parameters, and intestinal histology in broiler\n                        chickens","volume":"17","author":"Amer","year":"2021","journal-title":"BMC Vet Res."},{"key":"key2.0261002113056e+13_B33","doi-asserted-by":"crossref","first-page":"745849","DOI":"10.3389\/fimmu.2021.745849","article-title":"Development and functional properties of intestinal mucus layer\n                        in poultry","volume":"12","author":"Duangnumsawang","year":"2021","journal-title":"Front Immunol."},{"key":"key2.0261002113056e+13_B34","doi-asserted-by":"crossref","first-page":"91","DOI":"10.3382\/ps.2010-00760","article-title":"Effects of dietary enzymes on performance and intestinal goblet\n                        cell number of broilers exposed to a live coccidia oocyst\n                        vaccine","volume":"90","author":"Walk","year":"2011","journal-title":"Poult Sci."},{"key":"key2.0261002113056e+13_B35","doi-asserted-by":"crossref","first-page":"23","DOI":"10.3382\/japr\/pfv002","article-title":"Effects of dietary distillers dried grains with solubles (DDGS)\n                        on growth performance, oxidative stress, and immune function in broiler\n                        chickens","volume":"24","author":"Min","year":"2015","journal-title":"J Appl Poult Res."},{"key":"key2.0261002113056e+13_B36","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1016\/j.mgene.2015.03.006","article-title":"Analysis of consequences of non-synonymous SNP in feed conversion\n                        ratio associated TGF-\u03b2 receptor type 3 gene in\n                        chicken","volume":"4","author":"Rasal","year":"2015","journal-title":"Meta Gene."}],"container-title":["Journal of Animal Science and Technology"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/www.ejast.org\/download\/download_pdf?doi=10.5187\/jast.2023.e90","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/www.ejast.org\/download\/download_pdf?doi=10.5187\/jast.2023.e90","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T02:47:50Z","timestamp":1790909270000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.ejast.org\/archive\/view_article?doi=10.5187\/jast.2023.e90"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,3]]},"references-count":36,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2024,3,31]]},"published-print":{"date-parts":[[2024,3]]}},"alternative-id":["10.5187\/jast.2023.e90"],"URL":"https:\/\/doi.org\/10.5187\/jast.2023.e90","relation":{},"ISSN":["2672-0191","2055-0391"],"issn-type":[{"value":"2672-0191","type":"print"},{"value":"2055-0391","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,3]]},"assertion":[{"value":"2023-08-06","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-08-29","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}}]}}