{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T03:53:31Z","timestamp":1790913211843,"version":"4.1.0"},"reference-count":44,"publisher":"Korean Society of Animal Science and Technology","issue":"5","license":[{"start":{"date-parts":[[2024,9,1]],"date-time":"2024-09-01T00:00:00Z","timestamp":1725148800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0"}],"funder":[{"name":"National Research Foundation of Korea","award":["2022R1I1A3070740"],"award-info":[{"award-number":["2022R1I1A3070740"]}],"id":[{"id":"https:\/\/ror.org\/013aysd81","id-type":"ROR","asserted-by":"crossref"}]}],"content-domain":{"domain":["www.ejast.org"],"crossmark-restriction":true},"short-container-title":["J Anim Sci Technol"],"published-print":{"date-parts":[[2024,9]]},"abstract":"<jats:p>Deoxynivalenol (DON) is the most common mycotoxin contaminant of food or feed\n                    worldwide and causes disease in animals. Lauric acid (LA) is a medium-chain\n                    fatty acid useful for barrier functions such as antimicrobial activity in the\n                    intestine of monogastric animals. However, the molecular mechanisms by which\n                    lauric acid exerts its effects on the deoxynivalenol-exposed small intestine\n                    have not been studied. We used an intestinal porcine epithelial cell line\n                    (IPEC-J2) as an in vitro model to explore the molecular\n                    mechanism of lauric acid in alleviating deoxynivalenol-induced damage. We found\n                    that lauric acid reversed deoxynivalenol-induced reduction in cell viability.\n                    Our quantitative real-time polymerase chain reaction results indicated that\n                    lauric acid alleviated deoxynivalenol-induced apoptosis through Annexin-V.\n                    Additionally, immunofluorescence and Western blotting showed that lauric acid\n                    attenuated deoxynivalenol-induced forkhead box O3 (FOXO3a) translocation into\n                    the nucleus. These results suggest that lauric acid attenuates forkhead box O3\n                    translocation in the small intestine damaged by deoxynivalenol, thereby reducing\n                    apoptosis. In conclusion, this study found that lauric acid alleviates\n                    deoxynivalenol-induced damage in intestinal porcine epithelial cell line through\n                    various molecular mechanisms.<\/jats:p>","DOI":"10.5187\/jast.2023.e92","type":"journal-article","created":{"date-parts":[[2023,9,12]],"date-time":"2023-09-12T20:38:02Z","timestamp":1694551082000},"page":"1010-1020","update-policy":"https:\/\/doi.org\/10.5187\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Lauric acid reduces apoptosis by inhibiting FOXO3a-signaling in\n                    deoxynivalenol-treated IPEC-J2 cells"],"prefix":"10.5187","volume":"66","author":[{"ORCID":"https:\/\/orcid.org\/0009-0004-2727-1246","authenticated-orcid":false,"given":"Na Yeon","family":"Kim","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/040c17130","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Animal Science and Biotechnology, Kyungpook National University, Sangju 37224, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0019-1834","authenticated-orcid":false,"given":"Sang In","family":"Lee","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/040c17130","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Animal Science and Biotechnology, Kyungpook National University, Sangju 37224, Korea"},{"id":[{"id":"https:\/\/ror.org\/040c17130","id-type":"ROR","asserted-by":"publisher"}],"name":"Research Institute for Innovative Animal Science, Kyungpook National University, Sangju 37224, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"3132","published-online":{"date-parts":[[2024,9,30]]},"reference":[{"key":"key2.0261002113225e+13_B1","article-title":"Effects of weaning on intestinal upper villus epithelial cells of\n                        piglets","volume":"11","author":"Yang","year":"2016","journal-title":"PLOS ONE"},{"key":"key2.0261002113225e+13_B2","doi-asserted-by":"crossref","first-page":"854","DOI":"10.5187\/jast.2021.e69","article-title":"MicroRNA expression profiling during the suckling-to-weaning\n                        transition in pigs","volume":"63","author":"Jang","year":"2021","journal-title":"J Anim Sci Technol."},{"key":"key2.0261002113225e+13_B3","doi-asserted-by":"crossref","first-page":"689","DOI":"10.1016\/j.envpol.2019.05.026","article-title":"Deoxynivalenol induced apoptosis and inflammation of IPEC-J2\n                        cells by promoting ROS production","volume":"251","author":"Kang","year":"2019","journal-title":"Environ Pollut."},{"key":"key2.0261002113225e+13_B4","doi-asserted-by":"crossref","first-page":"1696","DOI":"10.4049\/jimmunol.1601221","article-title":"Cathelicidin-WA improves intestinal epithelial barrier function\n                        and enhances host defense against enterohemorrhagic Escherichia coli O157:H7\n                        infection","volume":"198","author":"Yi","year":"2017","journal-title":"J Immunol."},{"key":"key2.0261002113225e+13_B5","doi-asserted-by":"crossref","first-page":"1314","DOI":"10.5187\/jast.2021.e109","article-title":"Effects of Bacillus-based probiotics on growth performance,\n                        nutrient digestibility, and intestinal health of weaned pigs","volume":"63","author":"Mun","year":"2021","journal-title":"J Anim Sci Technol."},{"key":"key2.0261002113225e+13_B6","doi-asserted-by":"crossref","first-page":"503","DOI":"10.1038\/nri3228","article-title":"Epithelial antimicrobial defence of the skin and\n                        intestine","volume":"12","author":"Gallo","year":"2012","journal-title":"Nat Rev Immunol."},{"key":"key2.0261002113225e+13_B7","doi-asserted-by":"crossref","first-page":"2777","DOI":"10.3390\/ijms20112777","article-title":"Progress in mycotoxins affecting intestinal mucosal barrier\n                        function","volume":"20","author":"Ren","year":"2019","journal-title":"Int J Mol Sci."},{"key":"key2.0261002113225e+13_B8","doi-asserted-by":"crossref","first-page":"619","DOI":"10.3390\/toxins12100619","article-title":"The compromised intestinal barrier induced by\n                        mycotoxins","volume":"12","author":"Gao","year":"2020","journal-title":"Toxins"},{"key":"key2.0261002113225e+13_B9","doi-asserted-by":"crossref","first-page":"114291","DOI":"10.1016\/j.ecoenv.2022.114291","article-title":"Deoxynivalenol triggers porcine intestinal tight junction\n                        disorder: insights from mitochondrial dynamics and mitophagy","volume":"248","author":"Zhang","year":"2022","journal-title":"Ecotoxicol Environ Saf."},{"key":"key2.0261002113225e+13_B10","doi-asserted-by":"crossref","first-page":"9834813","DOI":"10.1155\/2020\/9834813","article-title":"Chloroquine improves deoxynivalenol-induced inflammatory response\n                        and intestinal mucosal damage in piglets","volume":"2020","author":"Liao","year":"2020","journal-title":"Oxid Med Cell Longev."},{"key":"key2.0261002113225e+13_B11","doi-asserted-by":"crossref","first-page":"983","DOI":"10.1007\/s00204-017-2083-x","article-title":"Intestinal toxicity of deoxynivalenol is limited by Lactobacillus\n                        rhamnosus RC007 in pig jejunum explants","volume":"92","author":"Garc\u00eda","year":"2018","journal-title":"Arch Toxicol."},{"key":"key2.0261002113225e+13_B12","doi-asserted-by":"crossref","first-page":"541","DOI":"10.3390\/toxins10120541","article-title":"Deoxynivalenol impairs porcine intestinal host defense peptide\n                        expression in weaned piglets and IPEC-J2 cells","volume":"10","author":"Wang","year":"2018","journal-title":"Toxins"},{"key":"key2.0261002113225e+13_B13","doi-asserted-by":"crossref","first-page":"111649","DOI":"10.1016\/j.fct.2020.111649","article-title":"The biological detoxification of deoxynivalenol: a\n                        review","volume":"145","author":"Yao","year":"2020","journal-title":"Food Chem Toxicol."},{"key":"key2.0261002113225e+13_B14","doi-asserted-by":"crossref","first-page":"617","DOI":"10.3390\/toxins11110617","article-title":"Decontamination of mycotoxin-contaminated feedstuffs and compound\n                        feed","volume":"11","author":"\u010colovi\u0107","year":"2019","journal-title":"Toxins"},{"key":"key2.0261002113225e+13_B15","doi-asserted-by":"crossref","first-page":"101","DOI":"10.1016\/S0300-483X(01)00471-1","article-title":"Toxicity, metabolism, and impact of mycotoxins on humans and\n                        animals","volume":"167","author":"Hussein","year":"2001","journal-title":"Toxicology"},{"key":"key2.0261002113225e+13_B16","doi-asserted-by":"crossref","first-page":"572","DOI":"10.2174\/1566524020666200128155115","article-title":"The effects of lauric acid on IPEC-J2 cell differentiation,\n                        proliferation, and death","volume":"20","author":"Yang","year":"2020","journal-title":"Curr Mol Med."},{"key":"key2.0261002113225e+13_B17","doi-asserted-by":"crossref","first-page":"58","DOI":"10.1016\/j.wasman.2020.07.050","article-title":"Rethinking organic wastes bioconversion: evaluating the potential\n                        of the black soldier fly (Hermetia illucens (L.)) (Diptera: stratiomyidae)\n                        (BSF)","volume":"117","author":"Surendra","year":"2020","journal-title":"Waste Manag."},{"key":"key2.0261002113225e+13_B18","doi-asserted-by":"crossref","first-page":"777","DOI":"10.2174\/1389203721666191231145901","article-title":"Effects of medium chain fatty acids on intestinal health of\n                        monogastric animals","volume":"21","author":"Jia","year":"2020","journal-title":"Curr Protein Pept Sci."},{"key":"key2.0261002113225e+13_B19","doi-asserted-by":"crossref","first-page":"skaa086","DOI":"10.1093\/jas\/skaa086","article-title":"Effects of dietary fatty acids on gut health and function of pigs\n                        pre- and post-weaning","volume":"98","author":"Lauridsen","year":"2020","journal-title":"J Anim Sci."},{"key":"key2.0261002113225e+13_B20","doi-asserted-by":"crossref","first-page":"44","DOI":"10.1186\/s40104-020-00446-1","article-title":"Medium-chain fatty acids and monoglycerides as feed additives for\n                        pig production: towards gut health improvement and feed pathogen\n                        mitigation","volume":"11","author":"Jackman","year":"2020","journal-title":"J Anim Sci Biotechnol."},{"key":"key2.0261002113225e+13_B21","doi-asserted-by":"crossref","first-page":"102825","DOI":"10.1016\/j.psj.2023.102825","article-title":"Effects of a combination of lauric acid monoglyceride and\n                        cinnamaldehyde on growth performance, gut morphology, and gut microbiota of\n                        yellow-feathered broilers","volume":"102","author":"Zheng","year":"2023","journal-title":"Poult Sci."},{"key":"key2.0261002113225e+13_B22","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0023871","article-title":"The mycotoxin deoxynivalenol potentiates intestinal inflammation\n                        by Salmonella typhimurium in porcine ileal loops","volume":"6","author":"Vandenbroucke","year":"2011","journal-title":"PLOS ONE"},{"key":"key2.0261002113225e+13_B23","doi-asserted-by":"crossref","first-page":"2379","DOI":"10.3390\/antiox11122379","article-title":"Plant-derived polyphenols as Nrf2 activators to counteract\n                        oxidative stress and intestinal toxicity induced by deoxynivalenol in swine:\n                        an emerging research direction","volume":"11","author":"Chen","year":"2022","journal-title":"Antioxidants"},{"key":"key2.0261002113225e+13_B24","doi-asserted-by":"crossref","first-page":"842","DOI":"10.5187\/jast.2022.e49","article-title":"Gene expression profiling after ochratoxin a treatment in small\n                        intestinal epithelial cells from pigs","volume":"64","author":"Yoon","year":"2022","journal-title":"J Anim Sci Technol."},{"key":"key2.0261002113225e+13_B25","doi-asserted-by":"crossref","first-page":"117865","DOI":"10.1016\/j.envpol.2021.117865","article-title":"Berberine improves intestinal barrier function and reduces\n                        inflammation, immunosuppression, and oxidative stress by regulating the\n                        NF-\u03baB\/MAPK signaling pathway in deoxynivalenol-challenged\n                        piglets","volume":"289","author":"Tang","year":"2021","journal-title":"Environ Pollut."},{"key":"key2.0261002113225e+13_B26","doi-asserted-by":"crossref","first-page":"9595","DOI":"10.1007\/s11033-020-06019-9","article-title":"Lauric acid alleviates insulin resistance by improving\n                        mitochondrial biogenesis in THP-1 macrophages","volume":"47","author":"Tham","year":"2020","journal-title":"Mol Biol Rep."},{"key":"key2.0261002113225e+13_B27","doi-asserted-by":"crossref","first-page":"101315","DOI":"10.1016\/j.psj.2021.101315","article-title":"Serum metabolome and gut microbiome alterations in broiler\n                        chickens supplemented with lauric acid","volume":"100","author":"Wu","year":"2021","journal-title":"Poult Sci."},{"key":"key2.0261002113225e+13_B28","doi-asserted-by":"crossref","first-page":"276","DOI":"10.1016\/j.aninu.2022.07.012","article-title":"Dietary butyrate, lauric acid and stearic acid improve gut\n                        morphology and epithelial cell turnover in weaned piglets","volume":"11","author":"Zeng","year":"2022","journal-title":"Anim Nutr."},{"key":"key2.0261002113225e+13_B29","doi-asserted-by":"crossref","first-page":"109640","DOI":"10.1016\/j.cbi.2021.109640","article-title":"Lauric acid alleviates deoxynivalenol-induced intestinal stem\n                        cell damage by potentiating the Akt\/mTORC1\/S6K1 signaling\n                        axis","volume":"348","author":"Liu","year":"2021","journal-title":"Chem Biol Interact."},{"key":"key2.0261002113225e+13_B30","doi-asserted-by":"crossref","first-page":"733","DOI":"10.3390\/toxins11120733","article-title":"Deoxynivalenol induces inflammatory injury in IPEC-J2 Cells via\n                        NF-\u03baB signaling pathway","volume":"11","author":"Wang","year":"2019","journal-title":"Toxins"},{"key":"key2.0261002113225e+13_B31","doi-asserted-by":"crossref","first-page":"BSR20180992","DOI":"10.1042\/BSR20180992","article-title":"Apoptosis and apoptotic body: disease message and therapeutic\n                        target potentials","volume":"39","author":"Xu","year":"2019","journal-title":"Biosci Rep."},{"key":"key2.0261002113225e+13_B32","doi-asserted-by":"crossref","first-page":"250762","DOI":"10.1155\/2015\/250762","article-title":"Apoptosis, necrosis, and necroptosis in the gut and intestinal\n                        homeostasis","volume":"2015","author":"Negroni","year":"2015","journal-title":"Mediators Inflamm."},{"key":"key2.0261002113225e+13_B33","doi-asserted-by":"crossref","first-page":"535","DOI":"10.3390\/toxics10090535","article-title":"Deoxynivalenol induces apoptosis via FOXO3a-signaling pathway in\n                        small-intestinal cells in pig","volume":"10","author":"Kang","year":"2022","journal-title":"Toxics"},{"key":"key2.0261002113225e+13_B34","doi-asserted-by":"crossref","first-page":"292","DOI":"10.1111\/j.1440-1843.2004.00602.x","article-title":"Mesothelial cell proliferation and apoptosis","volume":"9","author":"Leard","year":"2004","journal-title":"Respirology"},{"key":"key2.0261002113225e+13_B35","first-page":"5202","article-title":"Liraglutide inhibits the proliferation and promotes the apoptosis\n                        of MCF-7 human breast cancer cells through downregulation of microRNA-27a\n                        expression","volume":"17","author":"Zhao","year":"2018","journal-title":"Mol Med Rep."},{"key":"key2.0261002113225e+13_B36","doi-asserted-by":"crossref","first-page":"147","DOI":"10.3389\/fphys.2013.00147","article-title":"Mitochondria and FOXO3: breath or die","volume":"4","author":"Hagenbuchner","year":"2013","journal-title":"Front Physiol."},{"key":"key2.0261002113225e+13_B37","doi-asserted-by":"crossref","first-page":"173","DOI":"10.2340\/00015555-2535","article-title":"Apoptosis may explain the pharmacological mode of action and\n                        adverse effects of isotretinoin, including teratogenicity","volume":"97","author":"Melnik","year":"2017","journal-title":"Acta Derm Venereol."},{"key":"key2.0261002113225e+13_B38","doi-asserted-by":"crossref","first-page":"297","DOI":"10.1016\/S0959-4388(00)00211-7","article-title":"Transcription-dependent and -independent control of neuronal\n                        survival by the PI3K\u2013Akt signaling pathway","volume":"11","author":"Brunet","year":"2001","journal-title":"Curr Opin Neurobiol."},{"key":"key2.0261002113225e+13_B39","doi-asserted-by":"crossref","DOI":"10.1083\/jcb.202201159","article-title":"Paradoxical roles of caspase-3 in regulating cell survival,\n                        proliferation, and tumorigenesis","volume":"221","author":"Eskandari","year":"2022","journal-title":"J Cell Biol."},{"key":"key2.0261002113225e+13_B40","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1038\/34112","article-title":"A caspase-activated DNase that degrades DNA during apoptosis, and\n                        its inhibitor ICAD","volume":"391","author":"Enari","year":"1998","journal-title":"Nature"},{"key":"key2.0261002113225e+13_B41","doi-asserted-by":"crossref","first-page":"1160","DOI":"10.1111\/febs.13970","article-title":"The caspase-activated DNase: apoptosis and beyond","volume":"284","author":"Larsen","year":"2017","journal-title":"FEBS J."},{"key":"key2.0261002113225e+13_B42","doi-asserted-by":"crossref","first-page":"3448","DOI":"10.1016\/j.bbamcr.2013.06.001","article-title":"Crosstalk between apoptosis, necrosis and\n                        autophagy","volume":"1833","author":"Nikoletopoulou","year":"2013","journal-title":"Biochim Biophys Acta Mol Cell Res."},{"key":"key2.0261002113225e+13_B43","doi-asserted-by":"crossref","first-page":"1978","DOI":"10.1016\/j.bbamcr.2011.03.010","article-title":"Akt, FoxO and regulation of apoptosis","volume":"1813","author":"Zhang","year":"2011","journal-title":"Biochim Biophys Acta Mol Cell Res."},{"key":"key2.0261002113225e+13_B44","doi-asserted-by":"crossref","first-page":"4677","DOI":"10.1128\/IAI.00227-08","article-title":"Tumor suppressor Foxo3a is involved in the regulation of\n                        lipopolysaccharide-induced interleukin-8 in intestinal HT-29\n                        cells","volume":"76","author":"Snoeks","year":"2008","journal-title":"Infect Immun."}],"container-title":["Journal of Animal Science and Technology"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/www.ejast.org\/download\/download_pdf?doi=10.5187\/jast.2023.e92","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/www.ejast.org\/download\/download_pdf?doi=10.5187\/jast.2023.e92","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T02:57:58Z","timestamp":1790909878000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.ejast.org\/archive\/view_article?doi=10.5187\/jast.2023.e92"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,9]]},"references-count":44,"journal-issue":{"issue":"5","published-online":{"date-parts":[[2024,9,30]]},"published-print":{"date-parts":[[2024,9]]}},"alternative-id":["10.5187\/jast.2023.e92"],"URL":"https:\/\/doi.org\/10.5187\/jast.2023.e92","relation":{},"ISSN":["2672-0191","2055-0391"],"issn-type":[{"value":"2672-0191","type":"print"},{"value":"2055-0391","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,9]]},"assertion":[{"value":"2023-06-01","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-08-31","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}}]}}