{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T21:16:31Z","timestamp":1792790191990,"version":"4.5.0"},"reference-count":10,"publisher":"Korean Society of Animal Science and Technology","issue":"3","license":[{"start":{"date-parts":[[2025,5,1]],"date-time":"2025-05-01T00:00:00Z","timestamp":1746057600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0"}],"funder":[{"name":"Ministry of Agriculture, Food and Rural Affairs","award":["RS-2024-0040347740982119420101"],"award-info":[{"award-number":["RS-2024-0040347740982119420101"]}]},{"name":"National Research Foundation of Korea","award":["NRF-RS-2023-00275307"],"award-info":[{"award-number":["NRF-RS-2023-00275307"]}],"id":[{"id":"https:\/\/ror.org\/013aysd81","id-type":"ROR","asserted-by":"crossref"}]}],"content-domain":{"domain":["www.ejast.org"],"crossmark-restriction":true},"short-container-title":["J Anim Sci Technol"],"published-print":{"date-parts":[[2025,5]]},"abstract":"<jats:p>Priestia megaterium (formerly Bacillus megaterium) is a gram-positive, aerobic, spore-forming bacterium found in a wide range of environmental niches. Here, we report the complete genome sequence of P. megaterium S188 isolated from soil, which can decrease hydrogen sulfide (H2S) levels and help reduce malodor generation in livestock farms. Putative genes related to sulfide assimilation and conversion were found in the genome of P. megaterium S188; among these, one O-acetylhomoserine (O-AH) desulfhydrase, two cysteine synthases-primarily related to the biosynthesis of sulfur-containing amino acids, five rhodanese or sulfurtransferases, and one nitrogen reductase were identified. The genomic information on P. megaterium S188 provides insights into the possible biodegradation or conversion mechanisms of sulfur-containing substances that cause malodors, which can help reduce odor generation. Furthermore, identification of the key genes or molecules responsible for H2S reduction would facilitate the optimization of the H2S-degrading ability of S188.<\/jats:p>","DOI":"10.5187\/jast.2024.e81","type":"journal-article","created":{"date-parts":[[2024,10,13]],"date-time":"2024-10-13T23:15:47Z","timestamp":1728861347000},"page":"714-717","update-policy":"https:\/\/doi.org\/10.5187\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Complete genome sequence of Priestia megaterium S188, a hydrogen sulfide-degrading bacterium"],"prefix":"10.5187","volume":"67","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9811-2972","authenticated-orcid":false,"given":"Sang Hoon","family":"Kim","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/058pdbn81","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Animal Biotechnology, Dankook University, Cheonan 31116, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0027-7416","authenticated-orcid":false,"given":"Ji Hoon","family":"Song","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/058pdbn81","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Animal Biotechnology, Dankook University, Cheonan 31116, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4937-118X","authenticated-orcid":false,"given":"Remilyn M.","family":"Mendoza","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/058pdbn81","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Animal Biotechnology, Dankook University, Cheonan 31116, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9241-1250","authenticated-orcid":false,"given":"Dae-Kyung","family":"Kang","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/058pdbn81","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Animal Biotechnology, Dankook University, Cheonan 31116, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"3132","published-online":{"date-parts":[[2025,5,31]]},"reference":[{"key":"key2.0261002113427e+13_B1","doi-asserted-by":"crossref","first-page":"85","DOI":"10.15250\/joie.2018.17.2.85","article-title":"Study on the reduction of livestock malodor using microbial agents-focusing on swine facilities","volume":"17","author":"Hong","year":"2018","journal-title":"J Odor Indoor Environ."},{"key":"key2.0261002113427e+13_B2","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1016\/j.shaw.2019.12.007","article-title":"Evaluation of short-term exposure levels on ammonia and hydrogen sulfide during manure-handling processes at livestock farms","volume":"11","author":"Park","year":"2020","journal-title":"Saf Health Work."},{"key":"key2.0261002113427e+13_B3","doi-asserted-by":"crossref","first-page":"93","DOI":"10.3109\/10408444.2014.971943","article-title":"Chronic low-level hydrogen sulfide exposure and potential effects on human health: a review of the epidemiological evidence","volume":"45","author":"Lewis","year":"2015","journal-title":"Crit Rev Toxicol."},{"key":"key2.0261002113427e+13_B4","unstructured":"KangDK\nKimSH\nOhJK\n\ninventor\nDankook University Cheonan Campus Industry-Academic Cooperation Group, assignee. Bacillus megaterium S188 strain having enzyme secretion activity and hydrogen sulfide odor removal activity and uses thereof\nKorean patent KR102196585B1\n2020\nDec\n30"},{"key":"key2.0261002113427e+13_B5","doi-asserted-by":"crossref","first-page":"24","DOI":"10.1016\/j.ygeno.2017.12.009","article-title":"Comparative genomic analysis of Lactobacillus mucosae LM1 identifies potential niche-specific genes and pathways for gastrointestinal adaptation","volume":"111","author":"Valeriano","year":"2019","journal-title":"Genomics."},{"key":"key2.0261002113427e+13_B6","doi-asserted-by":"crossref","first-page":"103961","DOI":"10.1016\/j.fm.2021.103961","article-title":"Impact of serine and serine synthesis genes on H2S release in Saccharomyces cerevisiae during wine fermentation","volume":"103","author":"Li","year":"2022","journal-title":"Food Microbiol."},{"key":"key2.0261002113427e+13_B7","doi-asserted-by":"crossref","first-page":"7631","DOI":"10.1128\/JB.187.22.7631-7638.2005","article-title":"Bacillus subtilis cysteine synthetase is a global regulator of the expression of genes involved in sulfur assimilation","volume":"187","author":"Albanesi","year":"2005","journal-title":"J Bacteriol."},{"key":"key2.0261002113427e+13_B8","doi-asserted-by":"crossref","first-page":"14026","DOI":"10.1074\/jbc.M117.790170","article-title":"Structural and biochemical analyses indicate that a bacterial persulfide dioxygenase\u2013rhodanese fusion protein functions in sulfur assimilation","volume":"292","author":"Motl","year":"2017","journal-title":"J Biol Chem."},{"key":"key2.0261002113427e+13_B9","doi-asserted-by":"crossref","first-page":"4542","DOI":"10.1021\/acs.biochem.5b00584","article-title":"Staphylococcus aureus CstB is a novel multidomain persulfide dioxygenase-sulfurtransferase involved in hydrogen sulfide detoxification","volume":"54","author":"Shen","year":"2015","journal-title":"Biochemistry."},{"key":"key2.0261002113427e+13_B10","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1007\/s10532-015-9720-y","article-title":"Sulfide oxidation and nitrate reduction for potential mitigation of H2S in landfills","volume":"26","author":"Fang","year":"2015","journal-title":"Biodegradation."}],"container-title":["Journal of Animal Science and Technology"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/www.ejast.org\/download\/download_pdf?doi=10.5187\/jast.2024.e81","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/www.ejast.org\/download\/download_pdf?doi=10.5187\/jast.2024.e81","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T02:51:54Z","timestamp":1790909514000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.ejast.org\/archive\/view_article?doi=10.5187\/jast.2024.e81"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,5]]},"references-count":10,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2025,5,31]]},"published-print":{"date-parts":[[2025,5]]}},"alternative-id":["10.5187\/jast.2024.e81"],"URL":"https:\/\/doi.org\/10.5187\/jast.2024.e81","relation":{},"ISSN":["2672-0191","2055-0391"],"issn-type":[{"value":"2672-0191","type":"print"},{"value":"2055-0391","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,5]]},"assertion":[{"value":"2024-07-08","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-08-21","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}}]}}