{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T21:17:34Z","timestamp":1792790254389,"version":"4.5.0"},"reference-count":55,"publisher":"Korean Society of Animal Science and Technology","issue":"3","license":[{"start":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T00:00:00Z","timestamp":1777593600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0"}],"funder":[{"name":"National Research Foundation of Korea","award":["RS-2023-00210634"],"award-info":[{"award-number":["RS-2023-00210634"]}],"id":[{"id":"https:\/\/ror.org\/013aysd81","id-type":"ROR","asserted-by":"crossref"}]}],"content-domain":{"domain":["www.ejast.org"],"crossmark-restriction":true},"short-container-title":["J Anim Sci Technol"],"published-print":{"date-parts":[[2026,5]]},"abstract":"<jats:p>This study aimed to identify functional nutrients that enhance barrier function\n                    of chicken intestinal epithelial cells and alleviate stress in chickens by\n                    evaluating the effects of six candidate materials: threonine, arginine, vitamin\n                    C, vitamin E, chromium, and zinc. Each treatment group was treated with 2, 20,\n                    and 200 \u03bcg\/mL. Among these, vitamin C and zinc demonstrated superior\n                    effects on chicken intestinal epithelial cell proliferation. Arginine and zinc\n                    effectively reduced (p &lt; 0.05) heat stress-related\n                    \u201cheat shock protein 70\u201d levels. However, none of the tested\n                    materials significantly impacted oxidative stress markers, such as nitric oxide\n                    and reactive oxygen species. Notably, vitamin C and zinc increased\n                        (p &lt; 0.05) transepithelial electrical resistance and\n                    decreased (p &lt; 0.05) fluorescein\n                    isothiocyanate\u2013dextran permeability, indicating their positive impact on\n                    barrier function of chicken intestinal epithelial cells. Additionally, threonine\n                    evidently promoted tight junction health during prolonged treatment. These\n                    findings suggest that threonine, vitamin C, and zinc help upregulate proteins\n                    associated with tight junction integrity. Taken together, amino acids, vitamin\n                    C, and zinc display promise as functional nutrients for enhancing intestinal\n                    barrier function and mitigating stress damage in chickens.<\/jats:p>","DOI":"10.5187\/jast.2025.e25","type":"journal-article","created":{"date-parts":[[2025,4,14]],"date-time":"2025-04-14T04:18:12Z","timestamp":1744604292000},"page":"795-813","update-policy":"https:\/\/doi.org\/10.5187\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Mitigation of oxidative stress in chicken intestinal epithelial cells\n                    by functional nutrients"],"prefix":"10.5187","volume":"68","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8861-6517","authenticated-orcid":false,"given":"Seung Yun","family":"Lee","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/00saywf64","id-type":"ROR","asserted-by":"publisher"}],"name":"Division of Animal Science, Institute of Agriculture & Life Science, Gyeongsang National University, Jinju, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-9717-0545","authenticated-orcid":false,"given":"Ho Gun","family":"Jang","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/00saywf64","id-type":"ROR","asserted-by":"publisher"}],"name":"Division of Animal Science, Institute of Agriculture & Life Science, Gyeongsang National University, Jinju, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-8564-3868","authenticated-orcid":false,"given":"Hye Won","family":"Lee","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/00saywf64","id-type":"ROR","asserted-by":"publisher"}],"name":"Division of Animal Science, Institute of Agriculture & Life Science, Gyeongsang National University, Jinju, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-9780-7821","authenticated-orcid":false,"given":"Dong Bin","family":"Kim","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/00saywf64","id-type":"ROR","asserted-by":"publisher"}],"name":"Division of Animal Science, Institute of Agriculture & Life Science, Gyeongsang National University, Jinju, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-3387-7330","authenticated-orcid":false,"given":"Hyo Jin","family":"Lee","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/00saywf64","id-type":"ROR","asserted-by":"publisher"}],"name":"Division of Animal Science, Institute of Agriculture & Life Science, Gyeongsang National University, Jinju, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-5605-0652","authenticated-orcid":false,"given":"Jin Hong","family":"Park","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/00saywf64","id-type":"ROR","asserted-by":"publisher"}],"name":"Division of Animal Science, Institute of Agriculture & Life Science, Gyeongsang National University, Jinju, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-8352-4182","authenticated-orcid":false,"given":"Ha Neul","family":"Lee","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/02wnxgj78","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Animal Science, Chungbuk National University, Cheongju, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-1849-053X","authenticated-orcid":false,"given":"Gyu Lim","family":"Yeom","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/02wnxgj78","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Animal Science, Chungbuk National University, Cheongju, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-1938-977X","authenticated-orcid":false,"given":"Ju Yeong","family":"Park","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/02wnxgj78","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Animal Science, Chungbuk National University, Cheongju, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-9151-0135","authenticated-orcid":false,"given":"Yeong Bin","family":"Kim","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/02wnxgj78","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Animal Science, Chungbuk National University, Cheongju, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0289-2949","authenticated-orcid":false,"given":"Jong Hyuk","family":"Kim","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/02wnxgj78","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Animal Science, Chungbuk National University, Cheongju, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"3132","published-online":{"date-parts":[[2026,5,31]]},"reference":[{"key":"key2.0261002113838e+13_B1","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1177\/0148607111434963","article-title":"Oxidative stress","volume":"36","author":"Preiser","year":"2012","journal-title":"J Parenter Enteral Nutr."},{"key":"key2.0261002113838e+13_B2","doi-asserted-by":"crossref","first-page":"235","DOI":"10.3390\/antiox8070235","article-title":"Antioxidant defence systems and oxidative stress in poultry\n                        biology: an update","volume":"8","author":"Surai","year":"2019","journal-title":"Antioxidants."},{"key":"key2.0261002113838e+13_B3","doi-asserted-by":"crossref","first-page":"60","DOI":"10.3389\/fvets.2019.00060","article-title":"Oxidative stress in the poultry gut: potential challenges and\n                        interventions","volume":"6","author":"Mishra","year":"2019","journal-title":"Front Vet Sci."},{"key":"key2.0261002113838e+13_B4","doi-asserted-by":"crossref","first-page":"6","DOI":"10.1016\/j.anifeedsci.2017.07.009","article-title":"Effect of microencapsulated sodium butyrate dietary\n                        supplementation on growth performance and intestinal barrier function of\n                        broiler chickens infected with necrotic enteritis","volume":"232","author":"Song","year":"2017","journal-title":"Anim Feed Sci Technol."},{"key":"key2.0261002113838e+13_B5","doi-asserted-by":"crossref","first-page":"407","DOI":"10.1007\/s00726-013-1500-6","article-title":"Functional amino acids in nutrition and health","volume":"45","author":"Wu","year":"2013","journal-title":"Amino Acids."},{"key":"key2.0261002113838e+13_B6","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1080\/01652176.2020.1857887","article-title":"Nutritional significance of amino acids, vitamins and minerals as\n                        nutraceuticals in poultry production and health \u2013 a comprehensive\n                        review","volume":"41","author":"Alagawany","year":"2021","journal-title":"Vet Q."},{"key":"key2.0261002113838e+13_B7","doi-asserted-by":"crossref","first-page":"358","DOI":"10.1093\/japr\/5.4.358","article-title":"L-threonine for poultry: a review","volume":"5","author":"Kidd","year":"1996","journal-title":"J Appl Poult Res."},{"key":"key2.0261002113838e+13_B8","doi-asserted-by":"crossref","first-page":"182","DOI":"10.1007\/BF01611398","article-title":"Mucin output in ileal digesta of pigs fed a protein-free\n                        diet","volume":"36","author":"Lien","year":"1997","journal-title":"Z Ernahrungswiss."},{"key":"key2.0261002113838e+13_B9","first-page":"257","article-title":"Intestinal uptake and metabolism of threonine: nutritional\n                        impact","volume":"17","author":"Stoll","year":"2006","journal-title":"Adv Pork Prod."},{"key":"key2.0261002113838e+13_B10","doi-asserted-by":"crossref","first-page":"149","DOI":"10.1038\/msb4100196","article-title":"Systems metabolic engineering of Escherichia coli for\n                        L\u2010threonine production","volume":"3","author":"Lee","year":"2007","journal-title":"Mol Syst Biol."},{"key":"key2.0261002113838e+13_B11","doi-asserted-by":"crossref","first-page":"937","DOI":"10.3382\/ps\/pex394","article-title":"Threonine, arginine, and glutamine: influences on intestinal\n                        physiology, immunology, and microbiology in broilers","volume":"97","author":"Bortoluzzi","year":"2018","journal-title":"Poult Sci."},{"key":"key2.0261002113838e+13_B12","doi-asserted-by":"crossref","first-page":"227","DOI":"10.1177\/0148607186010002227","article-title":"Arginine: biochemistry, physiology, and therapeutic\n                        implications","volume":"10","author":"Barbul","year":"1986","journal-title":"J Parenter Enteral Nutr."},{"key":"key2.0261002113838e+13_B13","doi-asserted-by":"crossref","first-page":"G795","DOI":"10.1152\/ajpgi.1991.260.6.G795","article-title":"Role of polyamines in gastrointestinal mucosal\n                        growth","volume":"260","author":"McCormack","year":"1991","journal-title":"Am J Physiol Gastrointest Liver Physiol."},{"key":"key2.0261002113838e+13_B14","doi-asserted-by":"crossref","first-page":"8","DOI":"10.1016\/j.livsci.2007.07.003","article-title":"Important roles for the arginine family of amino acids in swine\n                        nutrition and production","volume":"112","author":"Wu","year":"2007","journal-title":"Livest Sci."},{"key":"key2.0261002113838e+13_B15","doi-asserted-by":"crossref","first-page":"1521","DOI":"10.1111\/jpn.12935","article-title":"Effects of dietary supplemental lysophospholipids and vitamin C\n                        on performance, antioxidant enzymes, lipid peroxidation, thyroid hormones\n                        and serum metabolites of broiler chickens reared under thermoneutral and\n                        high ambient temperature","volume":"102","author":"Zangeneh","year":"2018","journal-title":"J Anim Physiol Anim Nutr."},{"key":"key2.0261002113838e+13_B16","doi-asserted-by":"crossref","first-page":"362","DOI":"10.1186\/s12887-020-02248-w","article-title":"Serum level and clinical significance of vitamin E in children\n                        with allergic rhinitis","volume":"20","author":"Wang","year":"2020","journal-title":"BMC Pediatr."},{"key":"key2.0261002113838e+13_B17","doi-asserted-by":"crossref","first-page":"171","DOI":"10.1016\/j.jtemb.2017.10.012","article-title":"Mechanism of insulin-like effect of chromium (III) ions on\n                        glucose uptake in C2C12 mouse myotubes involves ROS\n                        formation","volume":"45","author":"Lipko","year":"2018","journal-title":"J Trace Elem Med Biol."},{"key":"key2.0261002113838e+13_B18","doi-asserted-by":"crossref","first-page":"4317","DOI":"10.3382\/ps\/pex249","article-title":"Effects of the supplemental chromium form on performance and\n                        oxidative stress in broilers exposed to heat stress","volume":"96","author":"Sahin","year":"2017","journal-title":"Poult Sci."},{"key":"key2.0261002113838e+13_B19","doi-asserted-by":"crossref","first-page":"651","DOI":"10.5713\/ajas.19.0089","article-title":"Chromium acetate stimulates adipogenesis through regulation of\n                        gene expression and phosphorylation of adenosine monophosphate-activated\n                        protein kinase in bovine intramuscular or subcutaneous\n                        adipocytes","volume":"33","author":"Kim","year":"2019","journal-title":"Asian-Australas J Anim Sci."},{"key":"key2.0261002113838e+13_B20","doi-asserted-by":"crossref","first-page":"992","DOI":"10.1016\/j.bbrc.2004.04.132","article-title":"A protective role for zinc on intestinal peroxidative damage\n                        during oral iron repletion","volume":"318","author":"Sreedhar","year":"2004","journal-title":"Biochem Biophys Res Commun."},{"key":"key2.0261002113838e+13_B21","doi-asserted-by":"crossref","first-page":"1800","DOI":"10.3390\/ijms20071800","article-title":"L-arginine inhibited inflammatory response and oxidative stress\n                        induced by lipopolysaccharide via arginase-1 signaling in IPEC-J2\n                        cells","volume":"20","author":"Qiu","year":"2019","journal-title":"Int J Mol Sci."},{"key":"key2.0261002113838e+13_B22","doi-asserted-by":"crossref","first-page":"5821","DOI":"10.1039\/D1FO00269D","article-title":"L-Threonine upregulates the expression of \u03b2-defensins by\n                        activating the NF-\u03baB signaling pathway and suppressing SIRT1\n                        expression in porcine intestinal epithelial cells","volume":"12","author":"Wang","year":"2021","journal-title":"Food Funct."},{"key":"key2.0261002113838e+13_B23","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1186\/1472-6882-1-11","article-title":"Dietary antioxidants protect gut epithelial cells from\n                        oxidant-induced apoptosis","volume":"1","author":"Miller","year":"2001","journal-title":"BMC Complement Altern Med."},{"key":"key2.0261002113838e+13_B24","doi-asserted-by":"crossref","first-page":"1614","DOI":"10.1016\/j.freeradbiomed.2005.02.029","article-title":"Effects of vitamin restriction and supplementation on rat\n                        intestinal epithelial cell apoptosis","volume":"38","author":"Vijayalakshhmi","year":"2005","journal-title":"Free Radic Biol Med."},{"key":"key2.0261002113838e+13_B25","doi-asserted-by":"crossref","first-page":"1040","DOI":"10.5187\/jast.2023.e22","article-title":"Effects of functional nutrients on chicken intestinal epithelial\n                        cells induced with oxidative stress","volume":"65","author":"Kim","year":"2023","journal-title":"J Anim Sci Technol."},{"key":"key2.0261002113838e+13_B26","doi-asserted-by":"crossref","first-page":"961","DOI":"10.1016\/j.freeradbiomed.2010.01.019","article-title":"Zinc inhibits oxidative stress-induced iron signaling and\n                        apoptosis in Caco-2 cells","volume":"48","author":"Kilari","year":"2010","journal-title":"Free Radic Biol Med."},{"key":"key2.0261002113838e+13_B27","doi-asserted-by":"crossref","first-page":"349","DOI":"10.4062\/biomolther.2013.064","article-title":"6\u2019-O-galloylpaeoniflorin protects human keratinocytes\n                        against oxidative stress-induced cell damage","volume":"21","author":"Yao","year":"2013","journal-title":"Biomol Ther."},{"key":"key2.0261002113838e+13_B28","doi-asserted-by":"crossref","first-page":"509","DOI":"10.1007\/s00418-015-1351-1","article-title":"Transepithelial electrical resistance (TEER): a functional\n                        parameter to monitor the quality of oviduct epithelial cells cultured on\n                        filter supports","volume":"144","author":"Chen","year":"2015","journal-title":"Histochem Cell Biol."},{"key":"key2.0261002113838e+13_B29","doi-asserted-by":"crossref","first-page":"101202","DOI":"10.1016\/j.psj.2021.101202","article-title":"Assay considerations for fluorescein isothiocyanate-dextran\n                        (FITC-d): an indicator of intestinal permeability in broiler\n                        chickens","volume":"100","author":"Liu","year":"2021","journal-title":"Poult Sci."},{"key":"key2.0261002113838e+13_B30","doi-asserted-by":"crossref","first-page":"215","DOI":"10.1016\/j.fct.2009.10.003","article-title":"The role of vitamin C as antioxidant in protection of oxidative\n                        stress induced by imidacloprid","volume":"48","author":"El-Gendy","year":"2010","journal-title":"Food Chem Toxicol."},{"key":"key2.0261002113838e+13_B31","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0120485","article-title":"Trolox and ascorbic acid reduce direct and indirect oxidative\n                        stress in the IPEC-J2 cells, an in vitro model for the porcine\n                        gastrointestinal tract","volume":"10","author":"Vergauwen","year":"2015","journal-title":"PLOS ONE."},{"key":"key2.0261002113838e+13_B32","doi-asserted-by":"crossref","first-page":"1447S","DOI":"10.1093\/jn\/130.5.1447S","article-title":"The antioxidant properties of zinc","volume":"130","author":"Powell","year":"2000","journal-title":"J Nutr."},{"key":"key2.0261002113838e+13_B33","doi-asserted-by":"crossref","first-page":"262","DOI":"10.1007\/s12011-022-03119-4","article-title":"Characteristics of Zn content and localization, Cu\u2013Zn SOD,\n                        and MT levels in the tissues of marginally Zn-deficient mice","volume":"201","author":"Wang","year":"2023","journal-title":"Biol Trace Elem Res."},{"key":"key2.0261002113838e+13_B34","doi-asserted-by":"crossref","first-page":"18","DOI":"10.1016\/j.jnutbio.2017.01.013","article-title":"Zinc enhances intestinal epithelial barrier function through the\n                        PI3K\/AKT\/mTOR signaling pathway in Caco-2 cells","volume":"43","author":"Shao","year":"2017","journal-title":"J Nutr Biochem."},{"key":"key2.0261002113838e+13_B35","doi-asserted-by":"crossref","first-page":"1397","DOI":"10.1016\/S1567-5769(01)00086-8","article-title":"Nitric oxide in immunity and inflammation","volume":"1","author":"Coleman","year":"2001","journal-title":"Int Immunopharmacol."},{"key":"key2.0261002113838e+13_B36","doi-asserted-by":"crossref","first-page":"H795","DOI":"10.1152\/ajpheart.00253.2017","article-title":"Vitamin C mitigates oxidative\/nitrosative stress and inflammation\n                        in doxorubicin-induced cardiomyopathy","volume":"313","author":"Akolkar","year":"2017","journal-title":"Am J Physiol Heart Circ Physiol."},{"key":"key2.0261002113838e+13_B37","doi-asserted-by":"crossref","first-page":"183","DOI":"10.1016\/S0163-7258(98)00028-X","article-title":"Heat shock protein 70 kDa: molecular biology, biochemistry, and\n                        physiology","volume":"80","author":"Kiang","year":"1998","journal-title":"Pharmacol Ther."},{"key":"key2.0261002113838e+13_B38","doi-asserted-by":"crossref","first-page":"R28","DOI":"10.1152\/ajpregu.1995.268.1.R28","article-title":"Tissue-specific HSP70 response in animals undergoing heat\n                        stress","volume":"268","author":"Flanagan","year":"1995","journal-title":"Am J Physiol Regul Integr Comp Physiol."},{"key":"key2.0261002113838e+13_B39","doi-asserted-by":"crossref","first-page":"565","DOI":"10.1002\/pmic.200600715","article-title":"Arginine deficiency in preconfluent intestinal Caco-2 cells\n                        modulates expression of proteins involved in proliferation, apoptosis, and\n                        heat shock response","volume":"7","author":"Lenaerts","year":"2007","journal-title":"Proteomics."},{"key":"key2.0261002113838e+13_B40","first-page":"129","article-title":"L-arginine decreases heat shock protein 70 [marker of\n                        environmental stress] expression in kidney cells of rat fetuses during\n                        apoptosis-late effect of adriamycin action","volume":"13","author":"Pedrycz","year":"2006","journal-title":"Ann Agric Environ Med."},{"key":"key2.0261002113838e+13_B41","doi-asserted-by":"crossref","first-page":"719","DOI":"10.5114\/aoms.2013.37273","article-title":"Influence of L-arginine on expression of HSP70 and p-53 proteins\n                        \u2013 early biomarkers of cellular danger in renal tubular\n                        cells","volume":"9","author":"Pedrycz","year":"2013","journal-title":"Immunohistochemical assessment. Arch Med Sci."},{"key":"key2.0261002113838e+13_B42","doi-asserted-by":"crossref","first-page":"592","DOI":"10.1093\/oxfordjournals.jbchem.a124222","article-title":"Regulation of hsp70 synthesis induced by cupric sulfate and zinc\n                        sulfate in thermotolerant HeLa cells","volume":"114","author":"Hatayama","year":"1993","journal-title":"J Biochem."},{"key":"key2.0261002113838e+13_B43","doi-asserted-by":"crossref","first-page":"621","DOI":"10.1016\/j.aninu.2021.01.003","article-title":"Zinc alleviates the heat stress of primary cultured hepatocytes\n                        of broiler embryos via enhancing the antioxidant ability and attenuating the\n                        heat shock responses","volume":"7","author":"Li","year":"2021","journal-title":"Anim Nutr."},{"key":"key2.0261002113838e+13_B44","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1007\/s11010-011-0998-8","article-title":"Inhibitory effect of SJSZ glycoprotein (38 kDa) on expression of\n                        heat shock protein 27 and 70 in chromium (VI)-treated\n                        hepatocytes","volume":"359","author":"Lee","year":"2012","journal-title":"Mol Cell Biochem."},{"key":"key2.0261002113838e+13_B45","first-page":"304","article-title":"Reactive oxygen species in health and disease","volume":"13","author":"Datta","year":"2000","journal-title":"Natl Med J India."},{"key":"key2.0261002113838e+13_B46","doi-asserted-by":"crossref","first-page":"111","DOI":"10.1201\/9780429289309-6","article-title":"Synergistic inhibition of oxidation by vitamin E and vitamin\n                        C","volume-title":"Cellular antioxidant defense mechanisms","author":"Niki","year":"2019"},{"key":"key2.0261002113838e+13_B47","doi-asserted-by":"crossref","first-page":"1179","DOI":"10.1016\/S0891-5849(01)00701-8","article-title":"Zinc resistance impairs sensitivity to oxidative stress in HeLa\n                        cells: protection through metallothioneins expression","volume":"31","author":"Chimienti","year":"2001","journal-title":"Free Radic Biol Med."},{"key":"key2.0261002113838e+13_B48","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1177\/2211068214561025","article-title":"TEER measurement techniques for in vitro barrier model\n                        systems","volume":"20","author":"Srinivasan","year":"2015","journal-title":"J Lab Autom."},{"key":"key2.0261002113838e+13_B49","doi-asserted-by":"crossref","first-page":"1181519","DOI":"10.3389\/fmicb.2023.1181519","article-title":"Effects of zinc oxide and condensed tannins on the growth\n                        performance and intestinal health of weaned piglets in ETEC-challenged\n                        environment","volume":"14","author":"Yi","year":"2023","journal-title":"Front Microbiol."},{"key":"key2.0261002113838e+13_B50","doi-asserted-by":"crossref","first-page":"1675","DOI":"10.1017\/S000711451600386X","article-title":"Effects of arginine on intestinal epithelial cell integrity and\n                        nutrient uptake","volume":"116","author":"Xia","year":"2016","journal-title":"Br J Nutr."},{"key":"key2.0261002113838e+13_B51","doi-asserted-by":"crossref","first-page":"677","DOI":"10.1002\/fsn3.3103","article-title":"The beneficial effects of alpha-tocopherol on intestinal function\n                        and the expression of tight junction proteins in differentiated segments of\n                        the intestine in piglets","volume":"11","author":"Huang","year":"2022","journal-title":"Food Sci Nutr."},{"key":"key2.0261002113838e+13_B52","doi-asserted-by":"crossref","first-page":"6020","DOI":"10.3390\/ijms20236020","article-title":"Structural features of tight-junction proteins","volume":"20","author":"Heinemann","year":"2019","journal-title":"Int J Mol Sci."},{"key":"key2.0261002113838e+13_B53","doi-asserted-by":"crossref","first-page":"235","DOI":"10.1186\/gb-2009-10-8-235","article-title":"The claudins","volume":"10","author":"Lal-Nag","year":"2009","journal-title":"Genome Biol."},{"key":"key2.0261002113838e+13_B54","doi-asserted-by":"crossref","first-page":"101138","DOI":"10.1016\/j.psj.2021.101138","article-title":"Research note: modified serum fluorescein isothiocyanate dextran\n                        (FITC-d) assay procedure to determine intestinal permeability in poultry fed\n                        diets high in natural or synthetic pigments","volume":"100","author":"Vuong","year":"2021","journal-title":"Poult Sci."},{"key":"key2.0261002113838e+13_B55","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1186\/s12986-021-00576-x","article-title":"Combined effect of vitamin C and vitamin D3 on intestinal\n                        epithelial barrier by regulating Notch signaling pathway","volume":"18","author":"Qiu","year":"2021","journal-title":"Nutr Metab."}],"container-title":["Journal of Animal Science and Technology"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/www.ejast.org\/download\/download_pdf?doi=10.5187\/jast.2025.e25","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/www.ejast.org\/download\/download_pdf?doi=10.5187\/jast.2025.e25","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T02:53:09Z","timestamp":1790909589000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.ejast.org\/archive\/view_article?doi=10.5187\/jast.2025.e25"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,5]]},"references-count":55,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2026,5,31]]},"published-print":{"date-parts":[[2026,5]]}},"alternative-id":["10.5187\/jast.2025.e25"],"URL":"https:\/\/doi.org\/10.5187\/jast.2025.e25","relation":{},"ISSN":["2672-0191","2055-0391"],"issn-type":[{"value":"2672-0191","type":"print"},{"value":"2055-0391","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,5]]},"assertion":[{"value":"2024-12-17","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-02-20","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}}]}}