{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T03:53:10Z","timestamp":1790913190622,"version":"4.1.0"},"reference-count":46,"publisher":"Korean Society of Animal Science and Technology","issue":"3","license":[{"start":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T00:00:00Z","timestamp":1777593600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0"}],"funder":[{"name":"Ministry of Science and ICT","award":["1711202880"],"award-info":[{"award-number":["1711202880"]}],"id":[{"id":"https:\/\/ror.org\/01wpjm123","id-type":"ROR","asserted-by":"crossref"}]},{"name":"Jeonbuk Advanced Bio Research &\n                                Development","award":["2024_JE_A_2"],"award-info":[{"award-number":["2024_JE_A_2"]}]}],"content-domain":{"domain":["www.ejast.org"],"crossmark-restriction":true},"short-container-title":["J Anim Sci Technol"],"published-print":{"date-parts":[[2026,5]]},"abstract":"<jats:p>Porphyromonas gingivalis is recognized for its significant\n                    association with periodontal diseases, encompassing conditions like gingivitis\n                    and periodontitis. P. gingivalis infiltrates periodontal\n                    tissues, liberating diverse outer membrane vesicles, notably lipopolysaccharide\n                    (LPS). These vesicles serve as triggers for innate immune responses, fostering\n                    inflammation. For this reason, LPS is commonly studied in research as a key tool\n                    for exploring microbiome infection and colonization dynamics. In the present\n                    study, we discovered a Canis familiaris Canine derived novel\n                    microbiome associated with the reduction of PG-LPS. We identified C.\n                        familiaris Canine derived microbiome, and we cultured candidate\n                    effective microbiome. Subsequently, in order to investigate the PG-LPS reducing\n                    effects of the microbiome, we conducted RAW 264.7 macrophage culture. We\n                    validated the expression patterns of inflammation marker genes on microbiome\n                    treatment in PG-LPS induced RAW 264.7. As a result, concentration of Nitric\n                    oxide, which were used for inflammation markers were decreased by candidate\n                    microbiome treatment. In addition, inflammation marker genes (interleukin 1 beta\n                        [IL1B], interleukin 6 [IL6], and tumor\n                    necrosis factor alpha [TNF-a]) were down regulated in\n                    microbiome and LPS co-treatment while it was up-regulated in RAW 264.7 cell\n                    induced with LPS as control group, which suggested that the candidate microbiome\n                    may have reduced the inflammation, but the mechanism in which this would have\n                    been done is yet known. Further studies should focus on elucidating the\n                    mechanism associated with candidate microbiomes and Inflammation reduction.<\/jats:p>","DOI":"10.5187\/jast.2025.e38","type":"journal-article","created":{"date-parts":[[2025,5,8]],"date-time":"2025-05-08T00:38:17Z","timestamp":1746664697000},"page":"875-888","update-policy":"https:\/\/doi.org\/10.5187\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Anti-inflammatory effect of Canis familiaris (dog)\n                    gingival derived microorganisms on Porphyromonas gingivalis\n                    derived lipopolysaccharide treated RAW 264.7 macrophage"],"prefix":"10.5187","volume":"68","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0885-3078","authenticated-orcid":false,"given":"Jeong-Woong","family":"Park","sequence":"first","affiliation":[{"name":"Center for Industrialization of Agricultural and Livestock Microorganisms (CIALM), Jeongeup, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-7215-841X","authenticated-orcid":false,"given":"Seon-Ae","family":"Choi","sequence":"additional","affiliation":[{"name":"Center for Industrialization of Agricultural and Livestock Microorganisms (CIALM), Jeongeup, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-6247-9107","authenticated-orcid":false,"given":"Jeong Won","family":"Kim","sequence":"additional","affiliation":[{"name":"Center for Industrialization of Agricultural and Livestock Microorganisms (CIALM), Jeongeup, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-4999-7970","authenticated-orcid":false,"given":"Jung-Hwan","family":"Ji","sequence":"additional","affiliation":[{"name":"Center for Industrialization of Agricultural and Livestock Microorganisms (CIALM), Jeongeup, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-6240-9853","authenticated-orcid":false,"given":"Kong-Min","family":"Kim","sequence":"additional","affiliation":[{"name":"Center for Industrialization of Agricultural and Livestock Microorganisms (CIALM), Jeongeup, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2190-9224","authenticated-orcid":false,"given":"Jun-Kyung","family":"Park","sequence":"additional","affiliation":[{"name":"Center for Industrialization of Agricultural and Livestock Microorganisms (CIALM), Jeongeup, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9352-9445","authenticated-orcid":false,"given":"Gui Hwan","family":"Han","sequence":"additional","affiliation":[{"name":"Center for Industrialization of Agricultural and Livestock Microorganisms (CIALM), Jeongeup, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2745-5227","authenticated-orcid":false,"given":"Seonghun","family":"Im","sequence":"additional","affiliation":[{"name":"Center for Industrialization of Agricultural and Livestock Microorganisms (CIALM), Jeongeup, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"3132","published-online":{"date-parts":[[2026,5,31]]},"reference":[{"key":"key2.0261002113854e+13_B1","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0009009","article-title":"A novel probiotic mixture exerts a therapeutic effect on\n                        experimental autoimmune encephalomyelitis mediated by IL-10 producing\n                        regulatory T cells","volume":"5","author":"Lavasani","year":"2010","journal-title":"PLOS ONE."},{"key":"key2.0261002113854e+13_B2","doi-asserted-by":"crossref","first-page":"10321","DOI":"10.1073\/pnas.0504084102","article-title":"Enhanced antiinflammatory capacity of a Lactobacillus plantarum\n                        mutant synthesizing modified teichoic acids","volume":"102","author":"Grangette","year":"2005","journal-title":"Proc Natl Acad Sci USA."},{"key":"key2.0261002113854e+13_B3","doi-asserted-by":"crossref","first-page":"203","DOI":"10.1203\/PDR.0b013e3181aabd4f","article-title":"Live and heat-killed Lactobacillus rhamnosus GG: effects on\n                        proinflammatory and anti-inflammatory cytokines\/chemokines in\n                        gastrostomy-fed infant rats","volume":"66","author":"Li","year":"2009","journal-title":"Pediatr Res."},{"key":"key2.0261002113854e+13_B4","doi-asserted-by":"crossref","first-page":"254","DOI":"10.1016\/j.cyto.2009.08.003","article-title":"Modulation of the immune response by probiotic strains in a mouse\n                        model of gluten sensitivity","volume":"48","author":"D\u2019Arienzo","year":"2009","journal-title":"Cytokine."},{"key":"key2.0261002113854e+13_B5","doi-asserted-by":"crossref","first-page":"S153","DOI":"10.1079\/BJN2002531","article-title":"Nutritional advantages of probiotics and\n                        prebiotics","volume":"87","author":"Marteau","year":"2002","journal-title":"Br J Nutr."},{"key":"key2.0261002113854e+13_B6","doi-asserted-by":"crossref","first-page":"889","DOI":"10.2174\/1389557043403369","article-title":"Cellular and physiological effects of probiotics and\n                        prebiotics","volume":"4","author":"Marteau","year":"2004","journal-title":"Mini Rev Med Chem."},{"key":"key2.0261002113854e+13_B7","doi-asserted-by":"crossref","first-page":"S23","DOI":"10.1016\/j.jfma.2018.08.011","article-title":"Fecal microbiota transplantation: review and\n                        update","volume":"118","author":"Wang","year":"2019","journal-title":"J Formos Med Assoc."},{"key":"key2.0261002113854e+13_B8","doi-asserted-by":"crossref","first-page":"322","DOI":"10.1902\/jop.1992.63.4s.322","article-title":"The bacterial etiology of destructive periodontal disease:\n                        current concepts","volume":"63","author":"Socransky","year":"1992","journal-title":"J Periodontol."},{"key":"key2.0261002113854e+13_B9","doi-asserted-by":"crossref","first-page":"12","DOI":"10.1111\/j.1600-0757.1997.tb00190.x","article-title":"The microbial challenge in periodontitis","volume":"14","author":"Darveau","year":"1997","journal-title":"Periodontology"},{"key":"key2.0261002113854e+13_B10","doi-asserted-by":"crossref","first-page":"101","DOI":"10.1111\/j.0906-6713.2004.003559.x","article-title":"Antigens of bacteria associated with\n                        periodontitis","volume":"35","author":"O\u2019Brien-Simpson","year":"2004","journal-title":"Periodontology"},{"key":"key2.0261002113854e+13_B11","doi-asserted-by":"crossref","first-page":"409","DOI":"10.2174\/1389203033487009","article-title":"Porphyromonas gingivalis gingipains: the molecular teeth of a\n                        microbial vampire","volume":"4","author":"O\u2019Brien-Simpson","year":"2003","journal-title":"Curr Protein Pept Sci."},{"key":"key2.0261002113854e+13_B12","doi-asserted-by":"crossref","DOI":"10.1128\/microbiolspec.PSIB-0001-2018","article-title":"Outer membrane vesicle-host cell interactions","volume":"7","author":"Cecil","year":"2019","journal-title":"Microbiol Spectr."},{"key":"key2.0261002113854e+13_B13","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1177\/1753425907088244","article-title":"Lipopolysaccharide (LPS) of porphyromonas gingivalis induces\n                        IL-1\u03b2, TNF-\u03b1 and IL-6 production by THP-1 cells in a way\n                        different from that of Escherichia coli LPS","volume":"14","author":"Zhang","year":"2008","journal-title":"Innate Immun."},{"key":"key2.0261002113854e+13_B14","doi-asserted-by":"crossref","first-page":"741","DOI":"10.1007\/s00011-022-01584-0","article-title":"Inflammatory biomarkers on an LPS-induced RAW 264.7 cell model: a\n                        systematic review and meta-analysis","volume":"71","author":"Facchin","year":"2022","journal-title":"Inflamm Res."},{"key":"key2.0261002113854e+13_B15","article-title":"Ethyl ferulate contributes to the inhibition of the inflammatory\n                        responses in murine RAW 264.7 macrophage cells and acute lung injury in\n                        mice","volume":"16","author":"Wang","year":"2021","journal-title":"PLOS ONE."},{"key":"key2.0261002113854e+13_B16","doi-asserted-by":"crossref","first-page":"4299","DOI":"10.1016\/j.ijbiomac.2020.09.036","article-title":"Sulfated polysaccharide from Turbinaria ornata suppress\n                        lipopolysaccharide-induced inflammatory response in RAW 264.7\n                        macrophages","volume":"164","author":"Bhardwaj","year":"2020","journal-title":"Int J Biol Macromol."},{"key":"key2.0261002113854e+13_B17","doi-asserted-by":"crossref","first-page":"23240","DOI":"10.1038\/srep23240","article-title":"Micheliolide inhibits LPS-induced inflammatory response and\n                        protects mice from LPS challenge","volume":"6","author":"Qin","year":"2016","journal-title":"Sci Rep."},{"key":"key2.0261002113854e+13_B18","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/1472-6882-8-1","article-title":"Ginger extract inhibits LPS induced macrophage activation and\n                        function","volume":"8","author":"Tripathi","year":"2008","journal-title":"BMC Complement Altern Med."},{"key":"key2.0261002113854e+13_B19","doi-asserted-by":"crossref","first-page":"8","DOI":"10.1038\/nrmicro2266","article-title":"The molecular basis of the host response to\n                        lipopolysaccharide","volume":"8","author":"Bryant","year":"2010","journal-title":"Nat Rev Microbiol."},{"key":"key2.0261002113854e+13_B20","doi-asserted-by":"crossref","first-page":"7155","DOI":"10.4049\/jimmunol.177.10.7155","article-title":"Lipopolysaccharide-activated CD4+ CD25+ T regulatory cells\n                        inhibit neutrophil function and promote their apoptosis and\n                        death","volume":"177","author":"Lewkowicz","year":"2006","journal-title":"J Immunol."},{"key":"key2.0261002113854e+13_B21","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1186\/1471-2172-9-65","article-title":"Gut microbiota and lipopolysaccharide content of the diet\n                        influence development of regulatory T cells: studies in germ-free\n                        mice","volume":"9","author":"Hrncir","year":"2008","journal-title":"BMC Immunol."},{"key":"key2.0261002113854e+13_B22","first-page":"3405","article-title":"Accelerated inflammation and oxidative stress induced by LPS in\n                        acute lung injury: inhibition by ST1926","volume":"41","author":"Dong","year":"2018","journal-title":"Int J Mol Med."},{"key":"key2.0261002113854e+13_B23","doi-asserted-by":"crossref","first-page":"863","DOI":"10.1016\/S1734-1140(13)71067-3","article-title":"LPS-induced oxidative stress and inflammatory reaction in the rat\n                        striatum","volume":"65","author":"Noworyta-Soko\u0142owska","year":"2013","journal-title":"Pharmacol Rep."},{"key":"key2.0261002113854e+13_B24","doi-asserted-by":"crossref","first-page":"1451","DOI":"10.4187\/respcare.05501451","article-title":"The antimicrobial effect of nitric oxide on the bacteria that\n                        cause nosocomial pneumonia in mechanically ventilated patients in the\n                        intensive care unit","volume":"50","author":"McMullin","year":"2005","journal-title":"Respir Care."},{"key":"key2.0261002113854e+13_B25","doi-asserted-by":"crossref","first-page":"300","DOI":"10.1046\/j.1365-2567.2000.00142.x","article-title":"Nitric oxide and virus infection","volume":"101","author":"Akaike","year":"2008","journal-title":"Immunology."},{"key":"key2.0261002113854e+13_B26","doi-asserted-by":"crossref","first-page":"1982","DOI":"10.1161\/01.CIR.101.16.1982","article-title":"Nitric oxide modulates expression of cell cycle regulatory\n                        proteins: a cytostatic strategy for inhibition of human vascular smooth\n                        muscle cell proliferation","volume":"101","author":"Tanner","year":"2000","journal-title":"Circulation"},{"key":"key2.0261002113854e+13_B27","doi-asserted-by":"crossref","first-page":"101550","DOI":"10.1016\/j.redox.2020.101550","article-title":"Nitric oxide in cellular adaptation and disease","volume":"34","author":"Gantner","year":"2020","journal-title":"Redox Biol."},{"key":"key2.0261002113854e+13_B28","doi-asserted-by":"crossref","first-page":"74","DOI":"10.1016\/0167-5699(94)90137-6","article-title":"The acute phase response","volume":"15","author":"Baumann","year":"1994","journal-title":"Immunol Today."},{"key":"key2.0261002113854e+13_B29","doi-asserted-by":"crossref","first-page":"240","DOI":"10.1128\/CMR.00046-08","article-title":"Pathogen recognition and inflammatory signaling in innate immune\n                        defenses","volume":"22","author":"Mogensen","year":"2009","journal-title":"Clin Microbiol Rev."},{"key":"key2.0261002113854e+13_B30","doi-asserted-by":"crossref","first-page":"2176","DOI":"10.3390\/ijms18102176","article-title":"Time response of oxidative\/nitrosative stress and inflammation in\n                        LPS-induced endotoxaemia\u2014a comparative study of mice and\n                        rats","volume":"18","author":"Steven","year":"2017","journal-title":"Int J Mol Sci."},{"key":"key2.0261002113854e+13_B31","doi-asserted-by":"crossref","first-page":"867","DOI":"10.3390\/ijms18040867","article-title":"Chemopreventive strategies for inflammation-related\n                        carcinogenesis: current status and future direction","volume":"18","author":"Kanda","year":"2017","journal-title":"Int J Mol Sci."},{"key":"key2.0261002113854e+13_B32","first-page":"2597","article-title":"Modified annexin V\/propidium iodide apoptosis assay for accurate\n                        assessment of cell death","volume":"50","author":"Rieger","year":"2011","journal-title":"J Vis Exp."},{"key":"key2.0261002113854e+13_B33","doi-asserted-by":"crossref","first-page":"954","DOI":"10.1016\/B978-0-12-384730-0.00399-2","article-title":"Non-thermal processing: irradiation","volume-title":"Encyclopedia of food microbiology","author":"Mendon\u00e7a","year":"2014"},{"key":"key2.0261002113854e+13_B34","doi-asserted-by":"crossref","first-page":"724","DOI":"10.1016\/j.tim.2019.03.008","article-title":"Bacillus subtilis","volume":"27","author":"Kov\u00e1cs","year":"2019","journal-title":"Trends Microbiol."},{"key":"key2.0261002113854e+13_B35","doi-asserted-by":"crossref","first-page":"13","DOI":"10.5772\/17184","article-title":"Bacillus-based biological control of plant\n                        diseases","volume-title":"Pesticides in the modern world","author":"Cawoy","year":"2011"},{"key":"key2.0261002113854e+13_B36","doi-asserted-by":"crossref","first-page":"1245","DOI":"10.1094\/PHYTO.2004.94.11.1245","article-title":"Taxonomy of the genus bacillus and related genera: the aerobic\n                        endospore-forming bacteria","volume":"94","author":"Fritze","year":"2004","journal-title":"Phytopathology."},{"key":"key2.0261002113854e+13_B37","doi-asserted-by":"crossref","first-page":"3669","DOI":"10.1007\/s00253-019-09710-5","article-title":"Bacillus velezensis: phylogeny, useful applications, and avenues\n                        for exploitation","volume":"103","author":"Adeniji","year":"2019","journal-title":"Appl Microbiol Biotechnol."},{"key":"key2.0261002113854e+13_B38","doi-asserted-by":"crossref","first-page":"943","DOI":"10.1126\/science.3933111","article-title":"Recombinant human tumor necrosis factor-\u03b1: effects on\n                        proliferation of normal and transformed cells in vitro","volume":"230","author":"Sugarman","year":"1985","journal-title":"Science."},{"key":"key2.0261002113854e+13_B39","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1016\/j.yexcr.2004.04.009","article-title":"Erythropoietin-dependent autocrine secretion of tumor necrosis\n                        factor-alpha in hematopoietic cells modulates proliferation via MAP\n                        kinase\u2013ERK-1\/2 and does not require tyrosine docking sites in the EPO\n                        receptor","volume":"298","author":"Chen","year":"2004","journal-title":"Exp Cell Res."},{"key":"key2.0261002113854e+13_B40","doi-asserted-by":"crossref","first-page":"6291","DOI":"10.1074\/jbc.M117.775023","article-title":"IL-1\u03b2-induced and p38MAPK-dependent activation of the\n                        mitogen-activated protein kinase-activated protein kinase 2 (MK2) in\n                        hepatocytes: signal transduction with robust and concentration-independent\n                        signal amplification","volume":"292","author":"Kulawik","year":"2017","journal-title":"J Biol Chem."},{"key":"key2.0261002113854e+13_B41","doi-asserted-by":"crossref","first-page":"1198","DOI":"10.1111\/jcpe.12995","article-title":"Effects of Bifidobacterium probiotic on the treatment of chronic\n                        periodontitis: a randomized clinical trial","volume":"45","author":"Invernici","year":"2018","journal-title":"J Clin Periodontol."},{"key":"key2.0261002113854e+13_B42","doi-asserted-by":"crossref","first-page":"529","DOI":"10.4103\/0976-237X.194114","article-title":"Assessment of Streptococcus mutans in healthy versus gingivitis\n                        and chronic periodontitis: a clinico-microbiological study","volume":"7","author":"Dani","year":"2016","journal-title":"Contemp Clin Dent."},{"key":"key2.0261002113854e+13_B43","doi-asserted-by":"crossref","first-page":"173","DOI":"10.1186\/s12934-020-01436-8","article-title":"Bacillus subtilis: a universal cell factory for industry,\n                        agriculture, biomaterials and medicine","volume":"19","author":"Su","year":"2020","journal-title":"Microb Cell Fact."},{"key":"key2.0261002113854e+13_B44","doi-asserted-by":"crossref","first-page":"108","DOI":"10.1016\/j.archoralbio.2016.02.014","article-title":"Favourable effects of Bacillus subtilis and Bacillus\n                        licheniformis on experimental periodontitis in rats","volume":"66","author":"Messora","year":"2016","journal-title":"Arch Oral Biol."},{"key":"key2.0261002113854e+13_B45","doi-asserted-by":"crossref","first-page":"1353","DOI":"10.1007\/s10096-009-0790-9","article-title":"The effect of Bacillus subtilis mouth rinsing in patients with\n                        periodontitis","volume":"28","author":"Tsubura","year":"2009","journal-title":"Eur J Clin Microbiol Infect Dis."},{"key":"key2.0261002113854e+13_B46","article-title":"Probiotic effect of Bacillus subtilis tablets on periodontopathic\n                        oral bacteria","volume":"3","author":"Tsubura","year":"2012","journal-title":"Microbiol Res."}],"container-title":["Journal of Animal Science and 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