{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T21:15:32Z","timestamp":1792790132110,"version":"4.5.0"},"reference-count":34,"publisher":"Korean Society of Animal Science and Technology","issue":"4","license":[{"start":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T00:00:00Z","timestamp":1782864000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0"}],"funder":[{"name":"National Research Foundation of Korea","award":["RS-2021-NR061941"],"award-info":[{"award-number":["RS-2021-NR061941"]}],"id":[{"id":"https:\/\/ror.org\/013aysd81","id-type":"ROR","asserted-by":"crossref"}]},{"name":"National Research Foundation of Korea","award":["RS-2022-NR069006"],"award-info":[{"award-number":["RS-2022-NR069006"]}],"id":[{"id":"https:\/\/ror.org\/013aysd81","id-type":"ROR","asserted-by":"crossref"}]},{"name":"National Research Foundation of Korea","award":["RS-2023-00246139"],"award-info":[{"award-number":["RS-2023-00246139"]}],"id":[{"id":"https:\/\/ror.org\/013aysd81","id-type":"ROR","asserted-by":"crossref"}]},{"name":"Ministry of Small and Medium-sized\n                                Enterprises","award":["RS-2023-00322055"],"award-info":[{"award-number":["RS-2023-00322055"]}]}],"content-domain":{"domain":["www.ejast.org"],"crossmark-restriction":true},"short-container-title":["J Anim Sci Technol"],"published-print":{"date-parts":[[2026,7]]},"abstract":"<jats:p>In this study, we investigated whether MIT-001, a small-molecule reactive oxygen\n                    species (ROS) scavenger, improves the re-expansion and viability of bovine\n                    blastocysts in response to vitrification-induced mitochondrial dysfunction and\n                    stress. Therefore, this study aimed to analyze the protective effects of MIT-001\n                    on the mitochondrial function of bovine blastocysts following vitrification\n                    warming. In this experiment, MIT-001 (0.1 \u03bcM) was allocated to three\n                    culture conditions based on treatment period: (I) warming only (WARM); (II)\n                    vitrification only (VITR); and (III) both vitrification and warming (VITR-WARM),\n                    compared with the control (Non-treated). Survival analysis of cryopreserved\n                    bovine blastocysts revealed that MIT-001 supplementation during the warming\n                    period (WARM group) significantly improved (p &lt; 0.01;\n                    Non-treated: 57.3 \u00b1 2.3% vs WARM: 74.2 \u00b1 7.3%) post-warm survival\n                    rates. It is noteworthy that surviving blastocysts in the WARM groups\n                    demonstrated significantly (p &lt; 0.05) lower TUNEL\n                    positive cells (%) and a higher ratio of expanded blastocyst development\n                    compared to the other groups. Intracellular ROS, as well as mitochondrial and\n                    nuclear superoxide levels, were significantly reduced (p\n                    &lt; 0.001) in the MIT-001-treated WARM group, accompanied by enhanced\n                    mitochondrial activation (MitoTracker Orange staining). Simultaneously,\n                    mitochondrial membrane potential, assessed using JC-1 staining, was elevated,\n                    whereas a reduction in mitochondrial fission marker dynamin-related protein 1\n                    (DRP1) expression was observed in surviving blastocysts from the\n                    MIT-001-supplemented WARM group (p &lt; 0.01). In addition,\n                    MIT-001 improved cytoskeletal stability by decreasing the aggregation thickness\n                    of filamentous actin (F-actin, p &lt; 0.001; Non-treated:\n                    10.73 \u03bcm vs. WARM: 6.03 \u03bcm) in bovine blastocyst of the WARM\n                    group. Finally, the enhanced developmental potential of vitrified-warmed\n                    blastocysts was linked to increased phospho-p38 mitogen-activated protein kinase\n                    (MAPK) expression exclusively in the WARM group compared to the other groups.\n                    Consequently, MIT-001 mitigates cryopreservation-induced cellular stress by\n                    improving mitochondrial function and regulates F-actin stabilization to enhance\n                    the viability and developmental potential of vitrified-warmed bovine\n                    blastocysts. These findings highlight the potential of MIT-001 to support\n                    cellular recovery and developmental capacity during cryopreservation, suggesting\n                    that it may play an effective protective role in bovine blastocyst\n                    cryopreservation.<\/jats:p>","DOI":"10.5187\/jast.2500079","type":"journal-article","created":{"date-parts":[[2025,8,20]],"date-time":"2025-08-20T02:20:32Z","timestamp":1755656432000},"page":"1066-1082","update-policy":"https:\/\/doi.org\/10.5187\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["MIT-001 mitigates vitrification-induced mitochondrial stress and\n                    regulates filamentous actin reorganization, enhancing the survival and\n                    development of vitrified-warmed bovine blastocysts"],"prefix":"10.5187","volume":"68","author":[{"ORCID":"https:\/\/orcid.org\/0009-0002-5616-930X","authenticated-orcid":false,"given":"Dae-Wook","family":"Kim","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01zqccq48","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Biotechnology, College of IT Engineering, Daegu University, Gyeongsan, Korea"},{"id":[{"id":"https:\/\/ror.org\/01zqccq48","id-type":"ROR","asserted-by":"publisher"}],"name":"DU Center for Infertility, Daegu University, Gyeongsan, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3287-2914","authenticated-orcid":false,"given":"Hyo-Jin","family":"Park","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01zqccq48","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Biotechnology, College of IT Engineering, Daegu University, Gyeongsan, Korea"},{"id":[{"id":"https:\/\/ror.org\/01zqccq48","id-type":"ROR","asserted-by":"publisher"}],"name":"DU Center for Infertility, Daegu University, Gyeongsan, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3247-2443","authenticated-orcid":false,"given":"Seul-Gi","family":"Yang","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01zqccq48","id-type":"ROR","asserted-by":"publisher"}],"name":"DU Center for Infertility, Daegu University, Gyeongsan, Korea"},{"id":[{"id":"https:\/\/ror.org\/01zqccq48","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Companion Animal Industry, College of Natural and Life Sciences, Daegu University, Gyeongsan, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8735-444X","authenticated-orcid":false,"given":"Jae-Ho","family":"Lee","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/013x1r993","id-type":"ROR","asserted-by":"publisher"}],"name":"CHA Fertility Center Seoul Station, Seoul, Korea"},{"id":[{"id":"https:\/\/ror.org\/04yka3j04","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Biomedical Sciences, College of Life Science, CHA University, Pocheon, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7825-9598","authenticated-orcid":false,"given":"Deog-Bon","family":"Koo","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01zqccq48","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Biotechnology, College of IT Engineering, Daegu University, Gyeongsan, Korea"},{"id":[{"id":"https:\/\/ror.org\/01zqccq48","id-type":"ROR","asserted-by":"publisher"}],"name":"DU Center for Infertility, Daegu University, Gyeongsan, Korea"},{"id":[{"id":"https:\/\/ror.org\/01zqccq48","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Companion Animal Industry, College of Natural and Life Sciences, Daegu University, Gyeongsan, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"3132","published-online":{"date-parts":[[2026,7,31]]},"reference":[{"key":"key2.026100211393e+13_B1","doi-asserted-by":"crossref","first-page":"5062","DOI":"10.3390\/ijms22105062","article-title":"MIT-001 restores human placenta-derived mesenchymal stem cells by\n                        enhancing mitochondrial quiescence and cytoskeletal\n                        organization","volume":"22","author":"Yu","year":"2021","journal-title":"Int J Mol Sci."},{"key":"key2.026100211393e+13_B2","doi-asserted-by":"crossref","first-page":"5223","DOI":"10.4049\/jimmunol.1402609","article-title":"The free radical scavenger NecroX-7 attenuates acute\n                        graft-versus-host disease via reciprocal regulation of Th1\/regulatory T\n                        cells and inhibition of HMGB1 release","volume":"194","author":"Im","year":"2015","journal-title":"J Immunol."},{"key":"key2.026100211393e+13_B3","doi-asserted-by":"crossref","first-page":"166","DOI":"10.1016\/j.atherosclerosis.2016.06.045","article-title":"NecroX-7 reduces necrotic core formation in atherosclerotic\n                        plaques of Apoe knockout mice","volume":"252","author":"Grootaert","year":"2016","journal-title":"Atherosclerosis."},{"key":"key2.026100211393e+13_B4","doi-asserted-by":"crossref","first-page":"531","DOI":"10.1111\/rda.12942","article-title":"Effect of vitrification on the mRNA transcriptome of bovine\n                        oocytes","volume":"52","author":"Wang","year":"2017","journal-title":"Reprod Domest Anim."},{"key":"key2.026100211393e+13_B5","doi-asserted-by":"crossref","first-page":"840","DOI":"10.1016\/j.bpj.2015.09.029","article-title":"Direct measurement of water states in cryopreserved cells reveals\n                        tolerance toward ice crystallization","volume":"110","author":"Huebinger","year":"2016","journal-title":"Biophys J."},{"key":"key2.026100211393e+13_B6","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1016\/S0378-4320(98)00098-0","article-title":"Vitrification of in vitro produced bovine blastocysts:\n                        methodological studies and developmental capacity","volume":"52","author":"Donnay","year":"1998","journal-title":"Anim Reprod Sci."},{"key":"key2.026100211393e+13_B7","doi-asserted-by":"crossref","first-page":"86","DOI":"10.1016\/j.cryobiol.2016.03.006","article-title":"Cryopreservation method affects DNA fragmentation in\n                        trophectoderm and the speed of re-expansion in bovine\n                        blastocysts","volume":"72","author":"Inaba","year":"2016","journal-title":"Cryobiology."},{"key":"key2.026100211393e+13_B8","doi-asserted-by":"crossref","first-page":"337","DOI":"10.3390\/antiox10030337","article-title":"Mitochondrial dysfunction and oxidative stress caused by\n                        cryopreservation in reproductive cells","volume":"10","author":"Gualtieri","year":"2021","journal-title":"Antioxidants."},{"key":"key2.026100211393e+13_B9","doi-asserted-by":"crossref","first-page":"378","DOI":"10.1262\/jrd.2013-015","article-title":"Comparison of ethylene glycol and propylene glycol for the\n                        vitrification of immature porcine oocytes","volume":"59","author":"Somfai","year":"2013","journal-title":"J Reprod Dev."},{"key":"key2.026100211393e+13_B10","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1262\/jrd.2014-070","article-title":"Mdivi-1, mitochondrial fission inhibitor, impairs developmental\n                        competence and mitochondrial function of embryos and cells in\n                        pigs","volume":"61","author":"Yeon","year":"2015","journal-title":"J Reprod Dev."},{"key":"key2.026100211393e+13_B11","doi-asserted-by":"crossref","first-page":"2245","DOI":"10.1091\/mbc.12.8.2245","article-title":"Dynamin-related protein Drp1 is required for mitochondrial\n                        division in mammalian cells","volume":"12","author":"Smirnova","year":"2001","journal-title":"Mol Biol Cell."},{"key":"key2.026100211393e+13_B12","doi-asserted-by":"crossref","first-page":"515","DOI":"10.1016\/S1534-5807(01)00055-7","article-title":"The role of dynamin-related protein 1, a mediator of\n                        mitochondrial fission, in apoptosis","volume":"1","author":"Frank","year":"2001","journal-title":"Dev Cell."},{"key":"key2.026100211393e+13_B13","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1038\/sj.cr.7290257","article-title":"Activation and signaling of the p38 MAP kinase\n                        pathway","volume":"15","author":"Zarubin","year":"2005","journal-title":"Cell Res."},{"key":"key2.026100211393e+13_B14","doi-asserted-by":"crossref","first-page":"629","DOI":"10.1042\/BC20040146","article-title":"p38 mitogen-activated protein kinase (MAPK) first regulates\n                        filamentous actin at the 8\u201316-cell stage during preimplantation\n                        development","volume":"97","author":"Paliga","year":"2005","journal-title":"Biol Cell."},{"key":"key2.026100211393e+13_B15","doi-asserted-by":"crossref","first-page":"1867","DOI":"10.3390\/biomedicines10081867","article-title":"Stabilization of F-actin cytoskeleton by paclitaxel improves the\n                        blastocyst developmental competence through P38 MAPK activity in porcine\n                        embryos","volume":"10","author":"Joe","year":"2022","journal-title":"Biomedicines."},{"key":"key2.026100211393e+13_B16","doi-asserted-by":"crossref","first-page":"299","DOI":"10.12750\/JARB.36.4.299","article-title":"Effects of Mito-TEMPO on the survival of vitrified bovine\n                        blastocysts in vitro","volume":"36","author":"Jeong","year":"2021","journal-title":"J Anim Reprod Biotechnol."},{"key":"key2.026100211393e+13_B17","doi-asserted-by":"crossref","first-page":"185","DOI":"10.1007\/s10815-014-0389-7","article-title":"Evaluation of the impact of vitrification on the actin\n                        cytoskeleton of in vitro matured ovine oocytes by means of Raman\n                        microspectroscopy","volume":"32","author":"Bogliolo","year":"2015","journal-title":"J Assist Reprod Genet."},{"key":"key2.026100211393e+13_B18","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0204571","article-title":"Resveratrol enhances the clearance of mitochondrial damage by\n                        vitrification and improves the development of vitrified-warmed bovine\n                        embryos","volume":"13","author":"Hara","year":"2018","journal-title":"PLOS ONE."},{"key":"key2.026100211393e+13_B19","doi-asserted-by":"crossref","first-page":"437","DOI":"10.1016\/j.theriogenology.2003.10.020","article-title":"Comparison on in vitro fertilized bovine embryos cultured in KSOM\n                        or SOF and cryopreserved by slow freezing or vitrification","volume":"62","author":"Nedambale","year":"2004","journal-title":"Theriogenology."},{"key":"key2.026100211393e+13_B20","doi-asserted-by":"crossref","first-page":"296","DOI":"10.1002\/jemt.20404","article-title":"Morphological changes in mouse embryos cryopreserved by different\n                        techniques","volume":"70","author":"Coutinho","year":"2007","journal-title":"Microsc Res Tech."},{"key":"key2.026100211393e+13_B21","doi-asserted-by":"crossref","first-page":"1072","DOI":"10.1071\/RD13405","article-title":"Effects of vitrification on the expression of pluripotency,\n                        apoptotic and stress genes in in vitro-produced porcine\n                        blastocysts","volume":"27","author":"Castillo-Mart\u00edn","year":"2015","journal-title":"Reprod Fertil Dev."},{"key":"key2.026100211393e+13_B22","doi-asserted-by":"crossref","first-page":"137","DOI":"10.1016\/j.anireprosci.2018.09.012","article-title":"Apoptosis and developmental capacity of vitrified parthenogenetic\n                        pig blastocysts","volume":"198","author":"Chen","year":"2018","journal-title":"Anim Reprod Sci."},{"key":"key2.026100211393e+13_B23","doi-asserted-by":"crossref","first-page":"10864","DOI":"10.3390\/ijms221910864","article-title":"Cryopreservation of gametes and embryos and their molecular\n                        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              cryopreservation","volume":"39","author":"Len","year":"2019","journal-title":"Biosci Rep."},{"key":"key2.026100211393e+13_B27","doi-asserted-by":"crossref","first-page":"R453","DOI":"10.1016\/j.cub.2014.03.034","article-title":"ROS function in redox signaling and oxidative\n                        stress","volume":"24","author":"Schieber","year":"2014","journal-title":"Curr Biol."},{"key":"key2.026100211393e+13_B28","doi-asserted-by":"crossref","first-page":"532","DOI":"10.1159\/000485089","article-title":"Antioxidants maintain cellular redox homeostasis by elimination\n                        of reactive oxygen species","volume":"44","author":"He","year":"2017","journal-title":"Cell Physiol Biochem."},{"key":"key2.026100211393e+13_B29","first-page":"4105","article-title":"To be or not to be?","volume":"11","author":"Szechy\u0144ska-Hebda","year":"2022","journal-title":"Are reactive oxygen species, antioxidants, and stress signalling\n                        universal determinants of life or death? Cells."},{"key":"key2.026100211393e+13_B30","doi-asserted-by":"crossref","first-page":"21517","DOI":"10.1038\/s41598-021-01082-z","article-title":"Metabolic activity in cryopreserved and grafted ovarian tissue\n                        using high-resolution respirometry","volume":"11","author":"Rodrigues","year":"2021","journal-title":"Sci Rep."},{"key":"key2.026100211393e+13_B31","doi-asserted-by":"crossref","first-page":"693969","DOI":"10.3389\/fcell.2021.693969","article-title":"Melatonin protects against Mdivi-1-induced abnormal spindle\n                        assembly and mitochondrial superoxide production during porcine oocyte\n                        maturation","volume":"9","author":"Yang","year":"2021","journal-title":"Front Cell Dev Biol."},{"key":"key2.026100211393e+13_B32","doi-asserted-by":"crossref","first-page":"315","DOI":"10.1017\/S0967199409990384","article-title":"Antioxidant treatment during preservation of bovine ovaries\n                        increased the development potential of embryos","volume":"18","author":"Nagao","year":"2010","journal-title":"Zygote."},{"key":"key2.026100211393e+13_B33","doi-asserted-by":"crossref","first-page":"15158","DOI":"10.3390\/ijms242015158","article-title":"Mitigating age-related ovarian dysfunction with the\n                        anti-inflammatory agent MIT-001","volume":"24","author":"Kang","year":"2023","journal-title":"Int J Mol Sci."},{"key":"key2.026100211393e+13_B34","doi-asserted-by":"crossref","first-page":"231","DOI":"10.12750\/JARB.37.4.231","article-title":"The antioxidant capacity of Mito-TEMPO improves the\n                        preimplantation development and viability of vitrified-warmed blastocysts\n                        through the stabilization of F-actin morphological aspects in bovine\n                        embryos","volume":"37","author":"Jeong","year":"2022","journal-title":"J Anim Reprod Biotechnol."}],"container-title":["Journal of Animal Science and Technology"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/www.ejast.org\/download\/download_pdf?doi=10.5187\/jast.2500079","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/www.ejast.org\/download\/download_pdf?doi=10.5187\/jast.2500079","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T02:57:58Z","timestamp":1790909878000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.ejast.org\/archive\/view_article?doi=10.5187\/jast.2500079"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,7]]},"references-count":34,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2026,7,31]]},"published-print":{"date-parts":[[2026,7]]}},"alternative-id":["10.5187\/jast.2500079"],"URL":"https:\/\/doi.org\/10.5187\/jast.2500079","relation":{},"ISSN":["2672-0191","2055-0391"],"issn-type":[{"value":"2672-0191","type":"print"},{"value":"2055-0391","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,7]]},"assertion":[{"value":"2025-03-11","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-07-05","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}}]}}