{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T07:52:28Z","timestamp":1791877948310,"version":"4.3.4"},"reference-count":45,"publisher":"Korean Society of Animal Science and Technology","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Anim Sci Technol"],"published-print":{"date-parts":[[2026,9]]},"abstract":"<jats:p>This study compared the fecal microbiota of Hanwoo steers exhibiting divergent\n                    residual feed intake (RFI) during the early fattening stage. In total, 63 Hanwoo\n                    steers, aged 16 months and maintained under identical dietary and environmental\n                    conditions, were evaluated for RFI based on individual feed intake and growth\n                    performance. From this cohort, high-RFI (inefficient; n = 5) and low-RFI\n                    (efficient; n = 6) steers representing the extreme 10% of the RFI distribution\n                    were selected for fecal microbiota analysis. Fresh fecal samples were collected\n                    and subjected to metataxonomic analysis using the Illumina MiSeq platform and\n                    QIIME2. At the phylum level, no significant differences were observed between\n                    the two steer groups. However, at the genus level, the cellulolytic\n                        Cellulosilyticum exhibited a significantly higher abundance\n                    in the low-RFI group, suggesting enhanced hindgut cellulose degradation in more\n                    efficient animals. Alpha-diversity metrics displayed no significant differences\n                    between the two steer groups. Beta-diversity analysis based on unweighted\n                    UniFrac distance revealed significant differences in fecal microbiota structure\n                    between the two groups, whereas weighted UniFrac analysis showed no significant\n                    differences, suggesting that while the two steer groups shared dominant\n                    microbiota members, they had distinct low-abundance, phylogenetically distinct\n                    taxa. Functional predictions using PICRUSt2 revealed enrichment of the\n                    ATP-binding cassette (ABC)-2 type transport system in the low-RFI group,\n                    implying greater microbial potential for nutrient uptake and utilization.\n                    Collectively, these findings suggest that feed efficiency in Hanwoo steers\n                    during early fattening is associated with specific fecal microbial taxa and\n                    functional traits rather than differences in overall diversity or dominant\n                    taxa.<\/jats:p>","DOI":"10.5187\/jast.2600072","type":"journal-article","created":{"date-parts":[[2026,4,27]],"date-time":"2026-04-27T00:16:22Z","timestamp":1777248982000},"page":"1517-1527","source":"Crossref","is-referenced-by-count":0,"title":["Divergent residual feed intake is associated with distinct fecal\n                    microbiota in early fattening Hanwoo steers"],"prefix":"10.5187","volume":"68","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2013-8210","authenticated-orcid":false,"given":"Cheolju","family":"Park","sequence":"first","affiliation":[{"name":"Division of Animal Science, Chonnam National University, Gwangju, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0062-3745","authenticated-orcid":false,"given":"Sang","family":"Yoon","sequence":"additional","affiliation":[{"name":"Division of Animal Science, Chonnam National University, Gwangju, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4027-5861","authenticated-orcid":false,"given":"Min-Seok","family":"Kim","sequence":"additional","affiliation":[{"name":"Division of Animal Science, Chonnam National University, Gwangju, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6165-8522","authenticated-orcid":false,"given":"Zhongtang","family":"Yu","sequence":"additional","affiliation":[{"name":"Department of Animal Sciences, The Ohio State University, Columbus, OH, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4480-4524","authenticated-orcid":false,"given":"Tansol","family":"Park","sequence":"additional","affiliation":[{"name":"Department of Animal Science and Technology, Chung-Ang University, Anseong, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8802-5661","authenticated-orcid":false,"given":"Minseok","family":"Kim","sequence":"additional","affiliation":[{"name":"Division of Animal Science, Chonnam National University, Gwangju, Korea"},{"name":"Department of Animal Sciences, The Ohio State University, Columbus, OH, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"3132","published-online":{"date-parts":[[2026,9,30]]},"reference":[{"key":"key2.0261013094154e+13_B1","doi-asserted-by":"crossref","first-page":"1815","DOI":"10.1017\/S1751731118000976","article-title":"Invited review: improving feed efficiency of beef cattle \u2013\n                        the current state of the art and future challenges","volume":"12","author":"Kenny","year":"2018","journal-title":"Animal"},{"key":"key2.0261013094154e+13_B2","doi-asserted-by":"crossref","first-page":"467","DOI":"10.2527\/jas.2013-6894","article-title":"Towards an improved estimation of the biological components of\n                        residual feed intake in growing cattle","volume":"92","author":"Savietto","year":"2014","journal-title":"J Anim Sci."},{"key":"key2.0261013094154e+13_B3","doi-asserted-by":"crossref","first-page":"758","DOI":"10.3390\/ani13040758","article-title":"Feeding management strategies to mitigate methane and improve\n                        production efficiency in feedlot cattle","volume":"13","author":"Galyean","year":"2023","journal-title":"Animals"},{"key":"key2.0261013094154e+13_B4","doi-asserted-by":"crossref","first-page":"885932","DOI":"10.3389\/fgene.2022.885932","article-title":"Selecting for feed efficient cows will help to reduce methane gas\n                        emissions","volume":"13","author":"Manzanilla-Pech","year":"2022","journal-title":"Front Genet."},{"key":"key2.0261013094154e+13_B5","doi-asserted-by":"crossref","first-page":"364","DOI":"10.5713\/ab.22.0382","article-title":"Assessment of the gastrointestinal microbiota using 16S ribosomal\n                        RNA gene amplicon sequencing in ruminant nutrition","volume":"36","author":"Kim","year":"2023","journal-title":"Anim Biosci."},{"key":"key2.0261013094154e+13_B6","doi-asserted-by":"crossref","first-page":"1515","DOI":"10.5713\/ajas.17.0544","article-title":"Metagenomic investigation of gastrointestinal microbiome in\n                        cattle","volume":"30","author":"Kim","year":"2017","journal-title":"Asian-Australas J Anim Sci."},{"key":"key2.0261013094154e+13_B7","doi-asserted-by":"crossref","first-page":"6","DOI":"10.1186\/s40168-019-0618-5","article-title":"Comparative metagenomic and metatranscriptomic analyses reveal\n                        the breed effect on the rumen microbiome and its associations with feed\n                        efficiency in beef cattle","volume":"7","author":"Li","year":"2019","journal-title":"Microbiome"},{"key":"key2.0261013094154e+13_B8","doi-asserted-by":"crossref","first-page":"92","DOI":"10.1186\/s40168-019-0699-1","article-title":"Host genetics influence the rumen microbiota and heritable rumen\n                        microbial features associate with feed efficiency in cattle","volume":"7","author":"Li","year":"2019","journal-title":"Microbiome"},{"key":"key2.0261013094154e+13_B9","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0129174","article-title":"Rumen microbiome from steers differing in feed\n                        efficiency","volume":"10","author":"Myer","year":"2015","journal-title":"PLOS ONE"},{"key":"key2.0261013094154e+13_B10","doi-asserted-by":"crossref","first-page":"1045","DOI":"10.1093\/jas\/skx081","article-title":"Rumen bacterial community structure impacts feed efficiency in\n                        beef cattle","volume":"96","author":"Paz","year":"2018","journal-title":"J Anim Sci."},{"key":"key2.0261013094154e+13_B11","doi-asserted-by":"crossref","first-page":"683","DOI":"10.2527\/jas.2013-6841","article-title":"Investigation of bacterial diversity in the feces of cattle fed\n                        different diets","volume":"92","author":"Kim","year":"2014","journal-title":"J Anim 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Ecol."},{"key":"key2.0261013094154e+13_B15","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1007\/s00284-015-0931-6","article-title":"A meta-analysis of bacterial diversity in the feces of\n                        cattle","volume":"72","author":"Kim","year":"2016","journal-title":"Curr Microbiol."},{"key":"key2.0261013094154e+13_B16","doi-asserted-by":"crossref","first-page":"101102","DOI":"10.1016\/j.animal.2024.101102","article-title":"Predicting feed efficiency of Angus steers using the\n                        gastrointestinal microbiome","volume":"18","author":"Congiu","year":"2024","journal-title":"Animal"},{"key":"key2.0261013094154e+13_B17","doi-asserted-by":"crossref","first-page":"1128","DOI":"10.3390\/microorganisms10061128","article-title":"Fecal microbiome differences in angus steers with differing feed\n                        efficiencies during the feedlot-finishing 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Res."},{"key":"key2.0261013094154e+13_B33","first-page":"32","article-title":"A new method for non-parametric multivariate analysis of\n                        variance","volume":"26","author":"Anderson","year":"2001","journal-title":"Austral Ecol."},{"key":"key2.0261013094154e+13_B34","doi-asserted-by":"crossref","first-page":"R60","DOI":"10.1186\/gb-2011-12-6-r60","article-title":"Metagenomic biomarker discovery and explanation","volume":"12","author":"Segata","year":"2011","journal-title":"Genome Biol."},{"key":"key2.0261013094154e+13_B35","doi-asserted-by":"crossref","first-page":"skaa230","DOI":"10.1093\/jas\/skaa230","article-title":"The impact of feed efficiency selection on the ruminal, cecal,\n                        and fecal microbiomes of Angus steers from a commercial\n                        feedlot","volume":"98","author":"Welch","year":"2020","journal-title":"J Anim Sci."},{"key":"key2.0261013094154e+13_B36","doi-asserted-by":"crossref","first-page":"845","DOI":"10.1099\/ijs.0.014712-0","article-title":"Cellulosilyticum ruminicola gen. nov., sp. nov., isolated from\n                        the rumen of yak, and reclassification of Clostridium lentocellum as\n                        Cellulosilyticum lentocellum comb. nov","volume":"60","author":"Cai","year":"2010","journal-title":"Int J Syst Evol Microbiol."},{"key":"key2.0261013094154e+13_B37","doi-asserted-by":"crossref","first-page":"1332497","DOI":"10.3389\/fmicb.2024.1332497","article-title":"Comparison of the gastrointestinal bacterial microbiota between\n                        dairy cows with and without mastitis","volume":"15","author":"Guo","year":"2024","journal-title":"Front Microbiol."},{"key":"key2.0261013094154e+13_B38","doi-asserted-by":"crossref","first-page":"23","DOI":"10.3390\/microorganisms12010023","article-title":"Competitive analysis of rumen and hindgut microbiota composition\n                        and fermentation function in diarrheic and non-diarrheic postpartum dairy\n                        cows","volume":"12","author":"Hao","year":"2024","journal-title":"Microorganisms"},{"key":"key2.0261013094154e+13_B39","doi-asserted-by":"crossref","first-page":"2345","DOI":"10.3390\/microorganisms10122345","article-title":"Degradation of cellulose and hemicellulose by ruminal\n                        microorganisms","volume":"10","author":"Weimer","year":"2022","journal-title":"Microorganisms"},{"key":"key2.0261013094154e+13_B40","doi-asserted-by":"crossref","first-page":"11254","DOI":"10.3168\/jds.2024-25003","article-title":"The fecal microbiota of Holstein cows is heritable and\n                        genetically correlated to dairy performances","volume":"107","author":"Brulin","year":"2024","journal-title":"J Dairy Sci."},{"key":"key2.0261013094154e+13_B41","doi-asserted-by":"crossref","first-page":"2306","DOI":"10.1038\/s41396-021-00925-x","article-title":"Host genetics exerts lifelong effects upon hindgut microbiota and\n                        its association with bovine growth and immunity","volume":"15","author":"Fan","year":"2021","journal-title":"ISME J."},{"key":"key2.0261013094154e+13_B42","doi-asserted-by":"crossref","first-page":"3063","DOI":"10.2527\/1996.74123063x","article-title":"Physical constraints on voluntary intake of forages by\n                        ruminants","volume":"74","author":"Allen","year":"1996","journal-title":"J Anim Sci."},{"key":"key2.0261013094154e+13_B43","doi-asserted-by":"crossref","first-page":"1415230","DOI":"10.3389\/fmicb.2024.1415230","article-title":"Survey of the fecal microbiota of indigenous small ruminants\n                        living in different areas of Guizhou","volume":"15","author":"Guo","year":"2024","journal-title":"Front Microbiol."},{"key":"key2.0261013094154e+13_B44","doi-asserted-by":"crossref","first-page":"276","DOI":"10.3390\/fermentation8060276","article-title":"Dynamic variations in rumen fermentation characteristics and\n                        bacterial community composition during in vitro fermentation","volume":"8","author":"Wei","year":"2022","journal-title":"Fermentation"},{"key":"key2.0261013094154e+13_B45","doi-asserted-by":"crossref","first-page":"5243","DOI":"10.1073\/pnas.1415037112","article-title":"Structural basis for substrate specificity of an amino acid ABC\n                        transporter","volume":"112","author":"Yu","year":"2015","journal-title":"Proc Natl Acad Sci."}],"container-title":["Journal of Animal Science and 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