{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T07:52:29Z","timestamp":1791877949893,"version":"4.3.4"},"reference-count":39,"publisher":"Korean Society of Animal Science and Technology","issue":"5","license":[{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0"}],"funder":[{"name":"Ministry of Agriculture, Food and Rural\n                                Affairs","award":["RS-2025-02216704"],"award-info":[{"award-number":["RS-2025-02216704"]}]}],"content-domain":{"domain":["www.ejast.org"],"crossmark-restriction":true},"short-container-title":["J Anim Sci Technol"],"published-print":{"date-parts":[[2026,9]]},"abstract":"<jats:p>Weaning is a critical developmental stage in piglets and is frequently associated\n                    with intestinal dysfunction, diarrhea, and growth retardation due to abrupt\n                    dietary and environmental changes. Probiotics have been explored as antibiotic\n                    alternatives to alleviate postweaning stress, and Lactobacillus\n                        plantarum has shown potential for modulating the gut microbiota and\n                    improving intestinal health. However, most previous studies have relied on\n                    single-omics approaches, limiting mechanistic understanding of\n                    host\u2013microbiome interactions. This study employed an integrated\n                    multi-omics approach combining ileal transcriptomics, fecal microbiomics, and\n                    metabolomics to investigate the effects of dietary L. plantarum\n                    supplementation in weaned piglets. Six piglets were assigned to either a control\n                    or L. plantarum-supplemented group for two weeks. RNA\n                    sequencing, 16S rRNA gene sequencing, and gas chromatography mass spectrometry\n                    based metabolomics were conducted, followed by integrative analysis using\n                    MIMOSA2 and MetaboAnalyst. Transcriptomic analysis identified 1,229\n                    differentially expressed genes enriched in immune-related pathways, including\n                    NF-\u03baB and IgA signaling, as well as epithelial barrier regulation.\n                    Microbiome profiling revealed increased microbial diversity, enrichment of\n                        Lactobacillus, and reduced abundance of\n                        Shigella. Functional prediction suggested enhanced innate\n                    immune responsiveness. Integrated metabolome\u2013transcriptome analysis\n                    highlighted lipid and amino acid metabolic pathways linking microbial\n                    metabolites with host gene expression. Overall, L. plantarum\n                    supplementation induced coordinated host\u2013microbiome adaptations\n                    associated with reduced oxidative stress, improved intestinal barrier stability,\n                    and enhanced immune resilience. These findings provide mechanistic evidence\n                    supporting L. plantarum as a promising nutritional strategy to\n                    mitigate postweaning stress and improve piglet intestinal health.<\/jats:p>","DOI":"10.5187\/jast.2600114","type":"journal-article","created":{"date-parts":[[2026,7,3]],"date-time":"2026-07-03T05:59:05Z","timestamp":1783058345000},"page":"1567-1585","update-policy":"https:\/\/doi.org\/10.5187\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["A multi-omics characterization of the gut microbiota of weaned\n                    piglets in response to Lactobacillus plantarum\n                    supplementation"],"prefix":"10.5187","volume":"68","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3719-9004","authenticated-orcid":false,"given":"Nae-Ho","family":"Park","sequence":"first","affiliation":[{"name":"Department of Animal Science and Technology, Chung-Ang University, Anseong, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8489-0044","authenticated-orcid":false,"given":"Byeonghwi","family":"Lim","sequence":"additional","affiliation":[{"name":"Department of Animal Science and Technology, Chung-Ang University, Anseong, Korea"},{"name":"Department of Animal Science, Iowa State University, Ames, IA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6272-4464","authenticated-orcid":false,"given":"Chiwoong","family":"Lim","sequence":"additional","affiliation":[{"name":"Department of Animal Science and Technology, Chung-Ang University, Anseong, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7520-7733","authenticated-orcid":false,"given":"Young-Jun","family":"Seo","sequence":"additional","affiliation":[{"name":"Department of Animal Science and Technology, Chung-Ang University, Anseong, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0775-7191","authenticated-orcid":false,"given":"Ji-Yeong","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Animal Science and Technology, Chung-Ang University, Anseong, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-7223-1506","authenticated-orcid":false,"given":"Sang-Hyeop","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Animal Science and Technology, Chung-Ang University, Anseong, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3836-729X","authenticated-orcid":false,"given":"Jungwoo","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Microbiology, College of Medicine, Dongguk University, Gyeongju, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0594-5279","authenticated-orcid":false,"given":"Minjee","family":"Lee","sequence":"additional","affiliation":[{"name":"IBS R&D Center, Ildong Bioscience, Pyeongtaek, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3590-3993","authenticated-orcid":false,"given":"Kyeong Il","family":"Park","sequence":"additional","affiliation":[{"name":"Department of Animal Science and Biotechnology, Chungnam National University, Daejeon, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5742-5374","authenticated-orcid":false,"given":"Hyunjin","family":"Kyoung","sequence":"additional","affiliation":[{"name":"Department of Animal Science and Biotechnology, Chungnam National University, Daejeon, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4515-5212","authenticated-orcid":false,"given":"Minho","family":"Song","sequence":"additional","affiliation":[{"name":"Department of Animal Science and Biotechnology, Chungnam National University, Daejeon, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6934-398X","authenticated-orcid":false,"given":"Jun-Mo","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Animal Science and Technology, Chung-Ang University, Anseong, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"3132","published-online":{"date-parts":[[2026,9,30]]},"reference":[{"key":"key2.0261013094154e+13_B1","doi-asserted-by":"crossref","first-page":"1042778","DOI":"10.3389\/fimmu.2022.1042778","article-title":"Weaning stress and intestinal health of piglets: a\n                        review","volume":"13","author":"Tang","year":"2022","journal-title":"Front Immunol."},{"key":"key2.0261013094154e+13_B2","doi-asserted-by":"crossref","first-page":"386","DOI":"10.1016\/j.aninu.2024.04.025","article-title":"Maternal probiotic mixture supplementation optimizes the gut\n                        microbiota structure of offspring piglets through the gut\u2013breast\n                        axis","volume":"19","author":"Hu","year":"2024","journal-title":"Anim Nutr."},{"key":"key2.0261013094154e+13_B3","doi-asserted-by":"crossref","first-page":"297","DOI":"10.1016\/j.aninu.2024.03.006","article-title":"Nutrition strategies to control post-weaning diarrhea of piglets:\n                        from the perspective of feeds","volume":"17","author":"Tang","year":"2024","journal-title":"Anim Nutr."},{"key":"key2.0261013094154e+13_B4","doi-asserted-by":"crossref","first-page":"331","DOI":"10.1016\/j.aninu.2017.06.007","article-title":"Using probiotics to improve swine gut health and nutrient\n                        utilization","volume":"3","author":"Liao","year":"2017","journal-title":"Anim Nutr."},{"key":"key2.0261013094154e+13_B5","doi-asserted-by":"crossref","first-page":"90","DOI":"10.3389\/fmicb.2019.00090","article-title":"PFM 105 promotes intestinal development through modulation of gut\n                        microbiota in weaning piglets","volume":"10","author":"Wang","year":"2019","journal-title":"Front Microbiol."},{"key":"key2.0261013094154e+13_B6","doi-asserted-by":"crossref","first-page":"1364","DOI":"10.3389\/fmicb.2018.01364","article-title":"Swine-derived probiotic Lactobacillus plantarum inhibits growth\n                        and adhesion of enterotoxigenic Escherichia coli and mediates host\n                        defense","volume":"9","author":"Wang","year":"2018","journal-title":"Front Microbiol."},{"key":"key2.0261013094154e+13_B7","doi-asserted-by":"crossref","first-page":"601394","DOI":"10.3389\/fvets.2021.601394","article-title":"The effects of feeding antibiotic on the intestinal microbiota of\n                        weanling pigs","volume":"8","author":"Lourenco","year":"2021","journal-title":"Front Vet Sci."},{"key":"key2.0261013094154e+13_B8","doi-asserted-by":"crossref","first-page":"795","DOI":"10.3390\/molecules23040795","article-title":"Antibiotic use in agriculture and its consequential resistance in\n                        environmental sources: potential public health implications","volume":"23","author":"Manyi-Loh","year":"2018","journal-title":"Molecules"},{"key":"key2.0261013094154e+13_B9","doi-asserted-by":"crossref","first-page":"e02345","DOI":"10.1128\/spectrum.02345-22","article-title":"Dietary supplementation with Lactiplantibacillus plantarum P-8\n                        improves the growth performance and gut microbiota of weaned\n                        piglets","volume":"12","author":"Yu","year":"2024","journal-title":"Microbiol 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l-serine\/l-threonine dehydratase with\n                        glutamate racemase activity in mammals","volume":"477","author":"Katane","year":"2020","journal-title":"Biochem J."},{"key":"key2.0261013094154e+13_B34","doi-asserted-by":"crossref","first-page":"361","DOI":"10.1016\/j.tem.2023.03.001","article-title":"Serine signaling governs metabolic homeostasis and\n                        health","volume":"34","author":"He","year":"2023","journal-title":"Trends Endocrinol Metab."},{"key":"key2.0261013094154e+13_B35","doi-asserted-by":"crossref","first-page":"1175","DOI":"10.1093\/glycob\/cwt054","article-title":"\u03b24GalT6 is involved in the synthesis of lactosylceramide\n                        with less intensity than \u03b24GalT5","volume":"23","author":"Tokuda","year":"2013","journal-title":"Glycobiology"},{"key":"key2.0261013094154e+13_B36","doi-asserted-by":"crossref","first-page":"E141","DOI":"10.4052\/tigg.1766.1E","article-title":"Glycosphingolipid-enriched lipid rafts-mediated pathogen\n                        recognition systems","volume":"31","author":"Nakayama","year":"2019","journal-title":"Trends Glycosci Glycotechnol."},{"key":"key2.0261013094154e+13_B37","doi-asserted-by":"crossref","first-page":"12","DOI":"10.1186\/s40104-016-0138-0","article-title":"The development and role of microbial-host interactions in gut\n                        mucosal immune development","volume":"8","author":"Stokes","year":"2017","journal-title":"J Anim Sci Biotechnol."},{"key":"key2.0261013094154e+13_B38","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0220843","article-title":"Beneficial roles of probiotics on the modulation of gut\n                        microbiota and immune response in pigs","volume":"14","author":"Shin","year":"2019","journal-title":"PLoS ONE"},{"key":"key2.0261013094154e+13_B39","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1016\/j.rvsc.2020.12.022","article-title":"Development of piglet gut microbiota at the time of weaning\n                        influences development of postweaning diarrhea \u2013 a field\n                        study","volume":"135","author":"Karasova","year":"2021","journal-title":"Res Vet Sci."}],"container-title":["Journal of Animal Science and Technology"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/www.ejast.org\/download\/download_pdf?doi=10.5187\/jast.2600114","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/www.ejast.org\/download\/download_pdf?doi=10.5187\/jast.2600114","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T07:44:06Z","timestamp":1791877446000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.ejast.org\/archive\/view_article?doi=10.5187\/jast.2600114"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9]]},"references-count":39,"journal-issue":{"issue":"5","published-online":{"date-parts":[[2026,9,30]]},"published-print":{"date-parts":[[2026,9]]}},"alternative-id":["10.5187\/jast.2600114"],"URL":"https:\/\/doi.org\/10.5187\/jast.2600114","relation":{},"ISSN":["2672-0191","2055-0391"],"issn-type":[{"value":"2672-0191","type":"print"},{"value":"2055-0391","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9]]},"assertion":[{"value":"2026-03-26","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication 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