{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,24]],"date-time":"2026-03-24T14:42:42Z","timestamp":1774363362352,"version":"3.50.1"},"reference-count":37,"publisher":"Copernicus GmbH","issue":"2","license":[{"start":{"date-parts":[[2013,2,20]],"date-time":"2013-02-20T00:00:00Z","timestamp":1361318400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"funder":[{"DOI":"10.13039\/501100000780","name":"European Commission","doi-asserted-by":"publisher","award":["211384"],"award-info":[{"award-number":["211384"]}],"id":[{"id":"10.13039\/501100000780","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biogeosciences"],"abstract":"<jats:p>Abstract. The effect of ocean acidification on the fatty acid composition of a natural plankton community in the Arctic was studied in a large-scale mesocosm experiment, carried out in Kongsfjorden (Svalbard, Norway) at 79\u00b0 N. Nine mesocosms of ~50 m3 each were exposed to 8 different pCO2 levels (from natural background conditions to ~1420 \u03bcatm), yielding pH values (on the total scale) from ~8.3 to 7.5. Inorganic nutrients were added on day 13. The phytoplankton development during this 30-day experiment passed three distinct phases: (1) prior to the addition of inorganic nutrients, (2) first bloom after nutrient addition, and (3) second bloom after nutrient addition. The fatty acid composition of the natural plankton community was analysed and showed, in general, high percentages of polyunsaturated fatty acids (PUFAs): 44\u201360% of total fatty acids. Positive correlations with pCO2 were found for most PUFAs during phases 2 and\/or 3, with the exception of 20:5n3 (eicosapentaenoic acid, EPA), an important diatom marker. These correlations are probably linked to changes in taxonomic composition in response to pCO2. While diatoms (together with prasinophytes and haptophytes) increased during phase 3 mainly in the low and intermediate pCO2 treatments, dinoflagellates were favoured by high CO2 concentrations during the same time period. This is reflected in the development of group-specific fatty acid trophic markers. No indications were found for a generally detrimental effect of ocean acidification on the planktonic food quality in terms of essential fatty acids.<\/jats:p>","DOI":"10.5194\/bg-10-1143-2013","type":"journal-article","created":{"date-parts":[[2013,2,20]],"date-time":"2013-02-20T11:25:50Z","timestamp":1361359550000},"page":"1143-1153","source":"Crossref","is-referenced-by-count":51,"title":["Effect of ocean acidification on the fatty acid composition of a natural plankton community"],"prefix":"10.5194","volume":"10","author":[{"given":"E.","family":"Leu","sequence":"first","affiliation":[]},{"given":"M.","family":"Daase","sequence":"additional","affiliation":[]},{"given":"K. 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C.: The role of highly unsaturated fatty acids in aquatic food web processes, Freshwater Biol., 38, 483\u2013499, 1997.","DOI":"10.1046\/j.1365-2427.1997.00220.x"},{"key":"ref4","doi-asserted-by":"crossref","unstructured":"Broecker, W. S., Takahashi, T., Simpson, H. J., and Peng, T. H.: Fate of fossil fuel carbon dioxide and the global carbon budget, Science, 26, 409\u2013418, 1979.","DOI":"10.1126\/science.206.4417.409"},{"key":"ref5","doi-asserted-by":"crossref","unstructured":"Brussaard, C. P. D., Noordeloos, A. A. M., Witte, H., Collenteur, M. C. J., Schulz, K., Ludwig, A., and Riebesell, U.: Arctic microbial community dynamics influenced by elevated CO2 levels, Biogeosciences, 10, 719\u2013731, https:\/\/doi.org\/10.5194\/bg-10-719-2013, 2013.","DOI":"10.5194\/bg-10-719-2013"},{"key":"ref6","doi-asserted-by":"crossref","unstructured":"Carvalho, A. P. and Malcata, F. X.: Optimization of omega-3 fatty acid production by microalgae: Crossover effects of co2 and light intensity under batch and continuous cultivation modes, Marine Biotechnol., 7, 381\u2013388, 2005.","DOI":"10.1007\/s10126-004-4047-4"},{"key":"ref7","doi-asserted-by":"crossref","unstructured":"Dalsgaard, J., St John, M., Kattner, G., Muller-Navarra, D., and Hagen, W.: Fatty acid trophic markers in the pelagic marine environment, Adv. Mar. Biol., 46, 225\u2013340, 2003.","DOI":"10.1016\/S0065-2881(03)46005-7"},{"key":"ref8","doi-asserted-by":"crossref","unstructured":"Fiorini, S., Gattuso, J.-P., van Rijswijk, P., and Middelburg, J.: Coccolithophores lipid and carbon isotope composition and their variability related to changes in seawater carbonate chemistry, J. Exp. Mar. Biol. Ecol., 394, 74\u201385, https:\/\/doi.org\/10.1016\/j.jembe.2010.07.020, 2010.","DOI":"10.1016\/j.jembe.2010.07.020"},{"key":"ref9","doi-asserted-by":"crossref","unstructured":"Folch, J., Lees, M., and Sloane Stanley, G. H.: A simple method for isolation and purification of total lipids from animal tissue, J. Biol. Chem., 226, 497\u2013509, 1957.","DOI":"10.1016\/S0021-9258(18)64849-5"},{"key":"ref10","doi-asserted-by":"crossref","unstructured":"Hoshida, H., Ohira, T., Minematsu, A., Akada, R., and Nishizawa, Y.: Accumulation of eicosapentaenoic acid in nannochloropsis sp in response to elevated co2 concentrations, J. Appl. Phycol., 17, 29\u201334, 10.1007\/s10811-005-5512-9, 2005.","DOI":"10.1007\/s10811-005-5512-9"},{"key":"ref11","doi-asserted-by":"crossref","unstructured":"Jonasdottir, S. H., Trung, N. H., Hansen, F., and Gartner, S.: Egg production and hatching success in the calanoid copepods calanus helgolandicus and calanus finmarchicus in the north sea from march to september 2001, J. Plankton Res., 27, 1239\u20131259, 2005.","DOI":"10.1093\/plankt\/fbi091"},{"key":"ref12","doi-asserted-by":"crossref","unstructured":"Jonasdottir, S. H., Visser, A. W., and Jespersen, C.: Assessing the role of food quality in the production and hatching of temora longicornis eggs, Mar. Ecol. Prog. Ser., 382, 139\u2013150, 2009.","DOI":"10.3354\/meps07985"},{"key":"ref13","doi-asserted-by":"crossref","unstructured":"Kainz, M., Arts, M. T., and Mazumder, A.: Essential fatty acids in the planktonic food web and their ecological role for higher trophic levels, Limnol. Oceanogr., 49, 1784\u20131793, 2004.","DOI":"10.4319\/lo.2004.49.5.1784"},{"key":"ref14","doi-asserted-by":"crossref","unstructured":"Klein Breteler, W. C. M., Schogt, N., and Rampen, S.: Effect of diatom nutrient limitation on copepod development: Role of essential lipids, Mar. Ecol. Prog. Ser., 291, 125\u2013133, 2005.","DOI":"10.3354\/meps291125"},{"key":"ref15","doi-asserted-by":"crossref","unstructured":"Leu, E., Falk-Petersen, S., Kwasniewski, S., Wulff, A., Edvardsen, K., and Hessen, D. O.: Fatty acid dynamics during the spring bloom in a high arctic fjord: Importance of abiotic factors versus community changes, Can. J. Fish. Aquat. Sci., 63, 2760\u20132779, 2006.","DOI":"10.1139\/f06-159"},{"key":"ref16","doi-asserted-by":"crossref","unstructured":"Leu, E., Wiktor, J., S\u00f8reide, J. E., Berge, J., and Falk-Petersen, S.: Increased irradiance reduces food quality of sea ice algae, Mar. Ecol. Prog. Ser., 411, 49\u201360, 2010.","DOI":"10.3354\/meps08647"},{"key":"ref17","doi-asserted-by":"crossref","unstructured":"Lischka, S., Buedenbender, J., Boxhammer, T., and Riebesell, U.: Impact of ocean acidification and elevated temperatures on early juveniles of the polar shelled pteropod limacina helicina: Mortality, shell degradation, and shell growth, Biogeosciences, 8, 919\u2013932, https:\/\/doi.org\/10.5194\/bg-8-919-2011, 2011.","DOI":"10.5194\/bg-8-919-2011"},{"key":"ref18","doi-asserted-by":"crossref","unstructured":"Mayzaud, P., Boutoute, M., and Gasparini, S.: Differential response of fatty acid composition in the different lipid classes from naturally occurring particulate organic matter during spring and summer in a high arctic fjord (Kongsfjorden, svalbard), submited, 2013.","DOI":"10.1016\/j.marchem.2013.02.009"},{"key":"ref19","doi-asserted-by":"crossref","unstructured":"M\u00fcller-Navarra, D. C.: Food web paradigms: The biochemical view on trophic interactions, Int. Rev. Hydrobiol., 93, 489\u2013505, https:\/\/doi.org\/10.1002\/iroh.200711046, 2008.","DOI":"10.1002\/iroh.200711046"},{"key":"ref20","doi-asserted-by":"crossref","unstructured":"Niehoff, B., Wolf, T., Czerny, J., and Boxhammer, T.: Mesozooplankton community development at elevated co2 concentrations: Results from a mesocosm experiment in a high arctic fjord, Biogeosciences, accepted, 10, 2013.","DOI":"10.5194\/bgd-9-11479-2012"},{"key":"ref21","doi-asserted-by":"crossref","unstructured":"Pond, D., Harris, R., Head, R., and Harbour, D.: Environmental and nutritional factors determining seasonal variability in the fecundity and egg viability of calanus helgolandicus in coastal waters off plymouth, uk, Mar. Ecol. Prog. Ser., 143, 45\u201363, 1996.","DOI":"10.3354\/meps143045"},{"key":"ref22","doi-asserted-by":"crossref","unstructured":"Reitan, K. I., Rainuzzo, J. R., and Olsen, Y.: Effect of nutrient limitation on fatty acid and lipid content of marine microalgae, J. Phycol., 30, 972\u2013979, 1994.","DOI":"10.1111\/j.0022-3646.1994.00972.x"},{"key":"ref23","doi-asserted-by":"crossref","unstructured":"Riebesell, U., Revill, A. T., Holdsworth, D. G., and Volkman, J. K.: The effects of varying CO2 concentration on lipid composition and carbon isotope fractionation in emiliania huxleyi, Geochim. Cosmochim. Ac., 64, 4179\u20134192, https:\/\/doi.org\/10.1016\/s0016-7037(00)00474-9, 2000.","DOI":"10.1016\/S0016-7037(00)00474-9"},{"key":"ref24","unstructured":"Riebesell, U., Br\u00f6ckel, K., B\u00fcdenbender, J., Czerny, J., Deckelnick, M., Hoffmann, D., Lenz, U., Ludwig, A., and Schulz, K. G.: A mobile, free-floating mesocosm platform for in situ experimentation, Biogeosciences, in preparation, 2013."},{"key":"ref25","doi-asserted-by":"crossref","unstructured":"Roessler, P. G.: Effects of silicon deficiency on lipid composition and metabolism in the diatom cyclotella cryptica, J. Phycol., 24, 394\u2013400, 1988.","DOI":"10.1111\/j.1529-8817.1988.tb00189.x"},{"key":"ref26","doi-asserted-by":"crossref","unstructured":"Rossoll, D., Bermudez, R., Hauss, H., Schulz, K. G., Riebesell, U., Sommer, U., and Winder, M.: Ocean acidification-induced food quality deterioration constrains trophic transfer, PLoS ONE, 7, e34737, 2012.","DOI":"10.1371\/journal.pone.0034737"},{"key":"ref27","unstructured":"Sargent, J. R., Bell, J. G., Bell, M. V., Henderson, R. J., and Tocher, D. R.: Dietary origins and functions of long-chain (n-3) polyunsaturated fatty acids in marine fish, J. Mar. Biotechnol., 3, 26\u201328, 1995."},{"key":"ref28","doi-asserted-by":"crossref","unstructured":"Sargent, J. R., McEvoy, L. A., and Bell, J. G.: Requirements, presentation and sources of polyunsaturated fatty acids in marine fish larval feeds, Aquaculture, 155, 117\u2013127, 1997.","DOI":"10.1016\/S0044-8486(97)00122-1"},{"key":"ref29","doi-asserted-by":"crossref","unstructured":"Schulz, K. G., Bellerby, R. G. J., Brussaard, C. P. D., B\u00fcdenbender, J., Czerny, J., Fischer, M., Koch-Klavsen, S., Krug, S., Lischka, S., Ludwig, A., M., M., Nondal, G., Silyakova, A., Stuhr, A., and Riebesell, U.: Temporal biomass dynamics of an arctic plankton bloom in response to increasing levels of atmospheric carbon dioxide, Biogeosciences, 10, 161\u2013180, 2013.","DOI":"10.5194\/bg-10-161-2013"},{"key":"ref30","doi-asserted-by":"crossref","unstructured":"Steinacher, M., Joos, F., Fr\u00f6licher, T. L., Plattner, G. K., and Doney, S. C.: Imminent ocean acidification in the arctic projected with the ncar global coupled carbon cycle-climate model, Biogeosciences, 6, 515\u2013533, 2009.","DOI":"10.5194\/bg-6-515-2009"},{"key":"ref31","doi-asserted-by":"crossref","unstructured":"Stillwell, W. and Wassall, S. R.: Docosahexaenoic acid: Membrane properties of a unique fatty acid, Chem. Phys. Lipids, 126, 1\u201327, 2003.","DOI":"10.1016\/S0009-3084(03)00101-4"},{"key":"ref32","doi-asserted-by":"crossref","unstructured":"Svendsen, H., Beszczynska-Moeller, A., Hagen, J. O., Lefauconnier, B., Tverberg, V., Gerland, S., Oerbaek, J. B., Bischof, K., Papucci, C., Zajaczkowski, M., Azzolini, R., Bruland, O., Wiencke, C., Winther, J. G., and Dallmann, W.: The physical environment of kongsfjorden-krossfjorden, an arctic fjord system in svalbard, Polar Res., 21, 133\u2013166, 2002.","DOI":"10.1111\/j.1751-8369.2002.tb00072.x"},{"key":"ref33","doi-asserted-by":"crossref","unstructured":"Thompson, P. A., Harrison, P. J., and Whyte, J. N. C.: Influence of irradiance on the fatty acid composition of phytoplankton, J. Phycol., 26, 278\u2013288, 1990.","DOI":"10.1111\/j.0022-3646.1990.00278.x"},{"key":"ref34","doi-asserted-by":"crossref","unstructured":"Thompson, P. A., Guo, M. X., Harrison, P. J., and Whyte, J. N. C.: Effects of variation in temperature on the fatty acid composition of 8 species of marine phytoplankton, J. Phycol., 28, 488\u2013497, 1992.","DOI":"10.1111\/j.0022-3646.1992.00488.x"},{"key":"ref35","doi-asserted-by":"crossref","unstructured":"Tsuzuki, M., Ohnuma, E., Sato, N., Takaku, T., and Kawaguchi, A.: Effects of co2 concentration during growth on fatty acid composition in microalgae, Plant Physiol., 93, 851\u2013856, https:\/\/doi.org\/10.1104\/pp.93.3.851, 1990.","DOI":"10.1104\/pp.93.3.851"},{"key":"ref36","doi-asserted-by":"crossref","unstructured":"Watanabe, T., Tamiya, T., Oka, A., Hirata, M., Kitajima, C., and Fujita, S.: Improvement of dietary value of live foods for fish larvae by feeding them on omega-3 highly unsaturated fatty-acids and fat soluble vitamins, B. Jpn. Soc. Sci. Fish., 49, 471\u2013479, 1983.","DOI":"10.2331\/suisan.49.471"},{"key":"ref37","doi-asserted-by":"crossref","unstructured":"Welschmeyer, N. A.: Fluorometric analysis of chlorophyll a in the presence of chlorophyll b and pheopigments, Limnol. Oceanogr., 39, 1985\u20131992, 1994.","DOI":"10.4319\/lo.1994.39.8.1985"}],"container-title":["Biogeosciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/bg.copernicus.org\/articles\/10\/1143\/2013\/bg-10-1143-2013.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,2,15]],"date-time":"2025-02-15T05:55:32Z","timestamp":1739598932000},"score":1,"resource":{"primary":{"URL":"https:\/\/bg.copernicus.org\/articles\/10\/1143\/2013\/"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,2,20]]},"references-count":37,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2013]]}},"URL":"https:\/\/doi.org\/10.5194\/bg-10-1143-2013","relation":{"has-preprint":[{"id-type":"doi","id":"10.5194\/bgd-9-8173-2012","asserted-by":"subject"},{"id-type":"doi","id":"10.5194\/bgd-9-8173-2012","asserted-by":"object"}]},"ISSN":["1726-4189"],"issn-type":[{"value":"1726-4189","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,2,20]]}}}