{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T01:10:12Z","timestamp":1792545012182,"version":"4.5.0"},"reference-count":38,"publisher":"Journal of Sports Science and Medicine","license":[{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"am","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["jsportscimed"],"abstract":"<jats:p>&lt;p&gt;Hydrogen-rich water (HRW) supplementation has been proposed to exert anti-fatigue effects during exercise; however, its impact on exercise performance and autonomic nervous system (ANS) function during repeated sprinting remains unclear. The aim of this study was to examine the effects of acute pre-exercise HRW ingestion on repeated sprint performance, ANS regulation, and blood lactate concentration. This randomized, single-blind, placebo-controlled crossover study included 13 male university athletes (23.9 \u00b1 2.0 years). Each participant completed two sessions of 7\u00d76-second all-out cycling sprints interspersed with 30-second recovery intervals, with a one-week washout period between sessions. Critical flicker fusion frequency, heart rate, heart rate variability (HRV), and blood lactate were assessed at baseline, during exercise, and at 0-5 min and 10 min post-exercise. Compared to the placebo trial, the HRW trial demonstrated significantly higher average power output (d = 0.61) and significantly lower total work decrement and fatigue index (d = 0.62; d = 0.64). Post-exercise HRV recovery was significantly accelerated in the HRW trial, including RMSSD (d = 0.62), LF\/HF ratio (d = 0.82), SampEn (d = 0.79), and DFA\u03b11 (d = 0.79). Blood lactate at 3 min post-exercise was approximately 1 mmol\/L lower in the HRW trial (d = 0.64). These findings suggest that acute HRW ingestion attenuates the decline in power output, accelerates ANS recovery, and enhances lactate clearance following repeated sprint exercise.&lt;\/p&gt;<\/jats:p>","DOI":"10.52082\/jssm.2026.744","type":"journal-article","created":{"date-parts":[[2026,8,4]],"date-time":"2026-08-04T13:09:26Z","timestamp":1785848966000},"page":"744-752","source":"Crossref","is-referenced-by-count":1,"title":["Effects of Hydrogen-Rich Water Supplementation on Exercise Performance, Autonomic Nervous System Recovery, and Blood Lactate Concentration During Repeated Sprint Exercise in Male University Athletes"],"prefix":"10.52082","author":[{"given":"Zhihao","family":"Chen","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meilan","family":"Chi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruizhi","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lianzhen","family":"Ma","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yupeng","family":"Shen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"29163","published-online":{"date-parts":[[2026,9,1]]},"reference":[{"key":"ref0","unstructured":"Abreu, R.M.d., Rehder-Santos, P., Sim&#x00F5;es, R.P., Catai, A.M. (2019) Can high-intensity interval training change cardiac autonomic control? A systematic review. Brazilian Journal of Physical Therapy 23, 279-289."},{"key":"ref1","unstructured":"Cai, W.-W., Zhang, M.-H., Yu, Y.-S., Cai, J.-H. (2012) Treatment with hydrogen molecule alleviates TNF&#x03B1;-induced cell injury in osteoblast. Molecular and Cellular Biochemistry 373, 1-9."},{"key":"ref2","unstructured":"Clemente-Su&#x00E1;rez, V.J., Diaz-Manzano, M. (2019) Evaluation of Central Fatigue by the Critical Flicker Fusion Threshold in Cyclists. Journal of Medical Systems 43, 61."},{"key":"ref3","unstructured":"Cohen, J. (1988) Statistical Power Analysis for the Behavioral Sciences.\r\n2nd ed. Hillsdale, NJ: Lawrence Erlbaum Associates, 24-27, 284-288."},{"key":"ref4","unstructured":"Da Ponte, A., Giovanelli, N., Nigris, D., Lazzer, S. (2018) Effects of hydrogen rich water on prolonged intermittent exercise. Journal of Sports Medicine and Physical Fitness 58, 612-621."},{"key":"ref5","unstructured":"Dohi, K., Kraemer, B.C., Erickson, M.A., McMillan, P.J., Kovac, A., Flachbartova, Z., Hansen, K.M., Shah, G.N., Sheibani, N., Salameh, T., Banks, W.A. (2014) Molecular Hydrogen in Drinking Water Protects against Neurodegenerative Changes Induced by Traumatic Brain Injury. PLOS ONE 9, e108034."},{"key":"ref6","unstructured":"Drid, P., Trivic, T., Casals, C., Trivic, S., Stojanovic, M., Ostojic, S.M. (2016) Is molecular hydrogen beneficial to enhance post-exercise recovery in female athletes? Science &#x0026; Sports 31, 207-213."},{"key":"ref7","unstructured":"Girard, O., Mendez-Villanueva, A., Bishop, D. (2011) Repeated-Sprint Ability &#x2013; Part I. Sports Medicine 41, 673-694."},{"key":"ref8","unstructured":"Hall, E.C.R., Lysenko, E.A., Semenova, E.A., Borisov, O.V., Andryushchenko, O.N., Andryushchenko, L.B., Vepkhvadze, T.F., Lednev, E.M., Zmijewski, P., Popov, D.V., Generozov, E.V., Ahmetov, I.I. (2021) Prediction of muscle fiber composition using multiple repetition testing. Biology of Sport 38, 277-283."},{"key":"ref9","unstructured":"Hong, Y., Dong, G., Li, Q., Wang, V., Liu, M., Jiang, G., Bao, D., Zhou, J. (2022) Effects of pre-exercise H2 inhalation on physical fatigue and related prefrontal cortex activation during and after high-intensity exercise. Frontiers in Physiology 13,"},{"key":"ref10","unstructured":"Hu, Q., Mei, Q.-Y., Huang, J.-L., Liu, W.-W., Manaenko, A., Sun, X.-J. (2019) Hydrogen inhibits microglial activation and regulates microglial phenotype in a mouse middle cerebral artery occlusion model. Medical Gas Research 9, 127-132."},{"key":"ref11","unstructured":"Aoki, K., Nakao, A., Adachi, T., Matsui, Y., Miyakawa, S. (2012) Pilot study: Effects of drinking hydrogen-rich water on muscle fatigue caused by acute exercise in elite athletes. Medical Gas Research 2, 12."},{"key":"ref12","unstructured":"Kawamura, T., Fujii, R., Higashida, K., Muraoka, I. (2019) Hydrogen water intake may suppress liver glycogen utilization without affecting redox biomarkers during exercise in rats."},{"key":"ref13","unstructured":"McKay, A.K.A., Stellingwerff, T., Smith, E.S., Martin, D.T., Mujika, I., Goosey-Tolfrey, V.L., Sheppard, J., Burke, L.M. (2022) Defining Training and Performance Caliber: A Participant Classification Framework. International Journal of Sports Physiology and Performance 17, 317-331."},{"key":"ref14","unstructured":"Mikami, T., Tano, K., Lee, H., Lee, H., Park, J., Ohta, F., LeBaron, T.W., Ohta, S. (2019) Drinking hydrogen water enhances endurance and relieves psychometric fatigue: a randomized, double-blind, placebo-controlled study. Canadian Journal of Physiology and Pharmacology 97, 857-862."},{"key":"ref15","unstructured":"Milioni, F., Zagatto, A., Barbieri, R., Andrade, V., dos Santos, J., Gobatto, C., da Silva, A., Santiago, P., Papoti, M. (2017) Energy Systems Contribution in the Running-based Anaerobic Sprint Test. International Journal of Sports Medicine 38, 226-232."},{"key":"ref16","unstructured":"Nie, J., Tong, T.K., Zhou, Y., Shi, Q., Zhang, H., Kong, Z. (2022) Cardiac autonomic disturbance following resistance and sprint-interval exercises in non-obese and obese young men. Applied Physiology, Nutrition, and Metabolism 47, 949-962."},{"key":"ref17","unstructured":"Ohsawa, I., Ishikawa, M., Takahashi, K., Watanabe, M., Nishimaki, K., Yamagata, K., Katsura, K.-i., Katayama, Y., Asoh, S., Ohta, S. (2007) Hydrogen acts as a therapeutic antioxidant by selectively reducing cytotoxic oxygen radicals. Nature Medicine 13, 688-694."},{"key":"ref18","unstructured":"Ohta, S. (2015) Molecular Hydrogen as a Novel Antioxidant. In: Cadenas,\r\nE. and Packer, L. (eds.) Hydrogen Sulfide in Redox Biology, Part\r\nB. Academic Press, 289-317."},{"key":"ref19","unstructured":"Ooi, C.H., Ng, S.K., Omar, E.A. (2020) Acute ingestion of hydrogen-rich water does not improve incremental treadmill running performance in endurance-trained athletes. Applied Physiology, Nutrition, and Metabolism 45, 513-519."},{"key":"ref20","unstructured":"Ostojic, S.M., Stojanovic, M.D. (2014) Hydrogen-Rich Water Affected Blood Alkalinity in Physically Active Men. Research in Sports Medicine 22, 49-60."},{"key":"ref21","unstructured":"Shi, Q., Tong, T.K., Sun, S., Kong, Z., Chan, C.K., Liu, W., Nie, J. (2018) Influence of recovery duration during 6-s sprint interval exercise on time spent at high rates of oxygen uptake. Journal of Exercise Science &#x0026; Fitness 16, 16-20."},{"key":"ref22","unstructured":"Aubert, A.E., Seps, B., Beckers, F. (2003) Heart Rate Variability in Athletes. Sports Medicine 33, 889-919."},{"key":"ref23","unstructured":"Sousa, N.M.F., Souza, M.N., Telaroli, D.S., Anjos, G.C., Machado, J.F., Baia, D.P., Pereira, J.J.M., Oliveira, J.N. (2015) Comparison of Heart Rate Variability After Constant and High Intensity Interval Training Exercise Sessions. Medicine &#x0026; Science in Sports &#x0026; Exercise 47, 798."},{"key":"ref24","unstructured":"Todorovic, N., Javorac, D., Stajer, V., Ostojic, S.M., Bosch, A. (2020) The Effects of Supersaturated Hydrogen-Rich Water Bathing on Biomarkers of Muscular Damage and Soreness Perception in Young Men Subjected to High-Intensity Eccentric Exercise. Journal of Sports Medicine 2020, 1-5."},{"key":"ref25","unstructured":"Tornero-Aguilera, J.F., Jimenez-Morcillo, J., Rubio-Zarapuz, A., Clemente-Su&#x00E1;rez, V.J. (2022) Central and Peripheral Fatigue in Physical Exercise Explained: A Narrative Review. International Journal of Environmental Research and Public Health 19, 3909."},{"key":"ref26","unstructured":"Ursua, A., Gandia, L.M., Sanchis, P. (2012) Hydrogen Production from Water Electrolysis: Current Status and Future Trends. Proceedings of the IEEE 100, 410-426."},{"key":"ref27","unstructured":"VanDusseldorp, T., Kerksick, C.M., Bustillo, E., Kalman, D. and Antonio, J. (2023) Proceedings of the Nineteenth International\r\nSociety of Sports Nutrition (ISSN) Conference and Expo. Journal of the International Society of Sports Nutrition 20, 2187955."},{"key":"ref28","unstructured":"Wang, Z., Jiang, F., Xiao, J., Chen, L., Zhang, Y., Li, J., Yi, Y., Min, W., Su, L., Liu, X., Zou, Z. (2022) Heart rate variability changes in patients with obstructive sleep apnea: A systematic review and meta-analysis. Journal of Sleep Research 32, e13708."},{"key":"ref29","unstructured":"Watanabe, Y., Mizuno, K., Sasaki, A., Ebisu, K., Tajima, K., Kajimoto, O., Nojima, J., Kuratsune, H., Hori, H. (2017) Hydrogen-rich water for improvements of mood, anxiety, and autonomic nerve function in daily life. Medical Gas Research 7, 247-255."},{"key":"ref30","unstructured":"Yoshimura, M., Nakamura, M., Kasahara, K., Yoshida, R., Murakami, Y., Hojo, T., Inoue, G., Makihira, N., Fukuoka, Y. (2023) Effect of CO2 and H2 gas mixture in cold water immersion on recovery after eccentric loading. Heliyon 9, e20288."},{"key":"ref31","unstructured":"Zhou, K., Liu, M., Wang, Y., Liu, H., Manor, B., Bao, D., Zhang, L., Zhou, J. (2023) Effects of molecular hydrogen supplementation on fatigue and aerobic capacity in healthy adults: A systematic review and meta-analysis. Frontiers in Nutrition 10,"},{"key":"ref32","unstructured":"Bellenger, C.R., Fuller, J.T., Thomson, R.L., Davison, K., Robertson, E.Y., Buckley, J.D. (2016) Monitoring Athletic Training Status Through Autonomic Heart Rate Regulation: A Systematic Review and Meta-Analysis. Sports Medicine 46, 1461-1486."},{"key":"ref33","unstructured":"Borg, G. (1985) An Introduction to Borg's RPE-Scale. Ithaca, NY:\r\nMouvement Publications."},{"key":"ref34","unstructured":"Botek, M., Khanna, D., Krej&#x010D;&#x00ED;, J., Valenta, M., McKune, A., Sl&#x00E1;de&#x010D;kov&#x00E1;, B., Klime&#x0161;ov&#x00E1;, I. (2022) Molecular Hydrogen Mitigates Performance Decrement during Repeated Sprints in Professional Soccer Players. Nutrients 14, 508."},{"key":"ref35","unstructured":"Botek, M., Krej&#x010D;&#x00ED;, J., McKune, A.J., Sl&#x00E1;de&#x010D;kov&#x00E1;, B. (2020) Hydrogen-Rich Water Supplementation and Up-Hill Running Performance: Effect of Athlete Performance Level. International Journal of Sports Physiology and Performance 15, 1193-1196."},{"key":"ref36","unstructured":"Botek, M., Sl&#x00E1;de&#x010D;kov&#x00E1;, B., Krej&#x010D;&#x00ED;, J., Pluh&#x00E1;&#x010D;ek, F., Najmanov&#x00E1;, E. (2021) Acute hydrogen-rich water ingestion stimulates cardiac autonomic activity in healthy females. Acta Gymnica 51, 04\/27."},{"key":"ref37","unstructured":"Buchheit, M. (2014) Monitoring training status with HR measures: do all roads lead to Rome? Frontiers in Physiology 5,"}],"container-title":["Journal of Sports Science and Medicine"],"original-title":[],"language":"en","deposited":{"date-parts":[[2026,8,4]],"date-time":"2026-08-04T13:09:26Z","timestamp":1785848966000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.jssm.org\/jssm-25-744.xml-Fulltext"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9,1]]},"references-count":38,"URL":"https:\/\/doi.org\/10.52082\/jssm.2026.744","archive":["Internet Archive"],"relation":{},"ISSN":["1303-2968"],"issn-type":[{"value":"1303-2968","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,1]]}}}