{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,14]],"date-time":"2025-05-14T13:14:56Z","timestamp":1747228496100,"version":"3.40.5"},"reference-count":0,"publisher":"Narra Sains Indonesia","issue":"2","license":[{"start":{"date-parts":[[2024,5,2]],"date-time":"2024-05-02T00:00:00Z","timestamp":1714608000000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Narra J"],"abstract":"<jats:p>\n\nThe expression of angiotensin-converting enzyme 2 (ACE2) in the adipose tissues of obese patients needs further study, as it may aid infection and serve as a viral reservoir. There has been controversy over whether to use ACE inhibitors to prevent coronavirus disease 2019 (COVID-19) severity. Perindopril, an ACE2 inhibitor, has been proposed; however, its relationship with COVID-19 has not yet been clear. The aim of this study was to investigate the effect of perindopril to reduce the expression of ACE2 and pro-inflammatory cytokine in adipocytes exposed to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Enzymatic isolation of adipose tissues was performed from obese male donor patients aged 30\u201350 years, then exposed it with SARS-CoV-2 S1 spike protein. This study also included human recombinant ACE2 (hrsACE2) as a comparison to perindopril. The expression of ACE2 was evaluated using ELISA. Our data indicated that SARS-CoV-2 Spike protein exposure increased ACE2 expression significantly. Administration of perindopril decreased ACE2 expression (43.37 \u00b5g\/mL) significantly compared to the positive group (80.31 \u00b5g\/mL) (p&lt;0.001). Perindopril administration also decreased IL-6 levels significantly compared to positive group(p&lt;0.001). \u00a0This study highlights that perindopril could reduce the ACE2 expression and pro-inflammatory cytokine levels in adipocytes exposed to SARS-CoV-2 S1 spike protein. \n\n<\/jats:p>","DOI":"10.52225\/narra.v4i2.746","type":"journal-article","created":{"date-parts":[[2024,5,3]],"date-time":"2024-05-03T04:22:05Z","timestamp":1714710125000},"page":"e746","source":"Crossref","is-referenced-by-count":0,"title":["Perindopril decreases angiotensin-converting enzyme 2 (ACE2) expression in human adipocytes exposed to SARS-CoV-2 S1 spike protein"],"prefix":"10.52225","volume":"4","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4063-684X","authenticated-orcid":false,"given":"Primasitha M.","family":"Harsoyo","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1706-6247","authenticated-orcid":false,"given":"Meity","family":"Ardiana","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2474-7558","authenticated-orcid":false,"given":"Hanestya O.","family":"Hermawan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5830-3571","authenticated-orcid":false,"given":"Yeni","family":"Purnamasari","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0650-6681","authenticated-orcid":false,"given":"Faizal A.","family":"Anandita","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"29794","published-online":{"date-parts":[[2024,5,2]]},"container-title":["Narra J"],"original-title":[],"link":[{"URL":"https:\/\/narraj.org\/main\/article\/download\/746\/352","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/narraj.org\/main\/article\/download\/746\/352","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,5,6]],"date-time":"2024-05-06T04:25:01Z","timestamp":1714969501000},"score":1,"resource":{"primary":{"URL":"https:\/\/narraj.org\/main\/article\/view\/746"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,5,2]]},"references-count":0,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2024,4,17]]}},"URL":"https:\/\/doi.org\/10.52225\/narra.v4i2.746","relation":{},"ISSN":["2807-2618"],"issn-type":[{"type":"electronic","value":"2807-2618"}],"subject":[],"published":{"date-parts":[[2024,5,2]]}}}