{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,23]],"date-time":"2026-06-23T10:23:00Z","timestamp":1782210180975,"version":"3.54.5"},"reference-count":68,"publisher":"Biophysical Society of China","issue":"6","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["swwlxb"],"published-print":{"date-parts":[[2025]]},"DOI":"10.52601\/bpr.2024.240047","type":"journal-article","created":{"date-parts":[[2024,12,12]],"date-time":"2024-12-12T03:31:38Z","timestamp":1733974298000},"page":"380","source":"Crossref","is-referenced-by-count":1,"title":["Cholesterol modulates vesicle clustering mediated by alpha-synuclein in a nonlinear fashion"],"prefix":"10.52601","volume":"11","author":[{"given":"Owen","family":"Tyoe","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chinta","family":"Aryal","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiajie","family":"Diao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"30428","reference":[{"key":"10.52601\/bpr.2024.240047.swwlxb.1","doi-asserted-by":"publisher","unstructured":"Aryal CM, Tyoe O, Diao J (2021) Lipid species dependent vesicles clustering caused by alpha-Synuclein as revealed by single-vesicle imaging with total internal reflection fluorescence microscopy. Biophys Rep 7(6): 437\u2212448","DOI":"10.52601\/bpr.2021.210020"},{"key":"10.52601\/bpr.2024.240047.swwlxb.2","doi-asserted-by":"publisher","unstructured":"Bu B, Crowe M, Diao J, Ji B, Li D (2018) Cholesterol suppresses membrane leakage by decreasing water penetrability. Soft Matter 14(25): 5277\u22125282","DOI":"10.1039\/C8SM00644J"},{"key":"10.52601\/bpr.2024.240047.swwlxb.3","doi-asserted-by":"publisher","unstructured":"Bu B, Tong X, Li D, Hu Y, He W, Zhao C, Hu R, Li X, Shao Y, Liu C, Zhao Q, Ji B, Diao J (2017) N-terminal acetylation preserves \u03b1-synuclein from oligomerization by blocking intermolecular hydrogen bonds. ACS Chem Neurosci 8(10): 2145\u22122151","DOI":"10.1021\/acschemneuro.7b00250"},{"key":"10.52601\/bpr.2024.240047.swwlxb.4","doi-asserted-by":"publisher","unstructured":"Bussi G, Donadio D, Parrinello M (2007) Canonical sampling through velocity rescaling. J Chem Phys 126(1): 014101. https:\/\/doi.org\/10.1063\/1.2408420","DOI":"10.1063\/1.2408420"},{"key":"10.52601\/bpr.2024.240047.swwlxb.5","doi-asserted-by":"publisher","unstructured":"Cai B, Liu J, Zhao Y, Xu X, Bu B, Li D, Zhang L, Dong W, Ji B, Diao J (2020) Single-vesicle imaging quantifies calcium's regulation of nanoscale vesicle clustering mediated by \u03b1-synuclein. Microsyst Nanoeng 6: 38. https:\/\/doi.org\/10.1038\/s41378-020-0147-1","DOI":"10.1038\/s41378-020-0147-1"},{"key":"10.52601\/bpr.2024.240047.swwlxb.6","doi-asserted-by":"publisher","unstructured":"Cai B, Yu L, Sharum SR, Zhang K, Diao J (2019) Single-vesicle measurement of protein-induced membrane tethering. Colloids Surf B Biointerfaces 177: 267\u2212273","DOI":"10.1016\/j.colsurfb.2019.02.004"},{"key":"10.52601\/bpr.2024.240047.swwlxb.7","doi-asserted-by":"publisher","unstructured":"Chamberlain LH, Burgoyne RD, Gould GW (2001) SNARE proteins are highly enriched in lipid rafts in PC12 cells: implications for the spatial control of exocytosis. Proc Natl Acad Sci USA 98(10): 5619\u22125624","DOI":"10.1073\/pnas.091502398"},{"key":"10.52601\/bpr.2024.240047.swwlxb.8","doi-asserted-by":"crossref","unstructured":"Clayton DF, George JM (1998) The synucleins: a family of proteins involved in synaptic function, plasticity, neurodegeneration and disease. TINS 21: 249\u2212254","DOI":"10.1016\/S0166-2236(97)01213-7"},{"key":"10.52601\/bpr.2024.240047.swwlxb.9","doi-asserted-by":"publisher","unstructured":"Clayton DF, George JM (1999) Synucleins in synaptic plasticity and neurodegenerative disorders. J Neurosci Res 58: 120\u2212129","DOI":"10.1002\/(SICI)1097-4547(19991001)58:1<120::AID-JNR12>3.0.CO;2-E"},{"key":"10.52601\/bpr.2024.240047.swwlxb.10","doi-asserted-by":"publisher","unstructured":"Conway KA, Harper JD, Lansbury PT Jr (2000) Fibrils formed <i>in vitro<\/i> from alpha-synuclein and two mutant forms linked to Parkinson's disease are typical amyloid. Biochemistry 39(10): 2552\u22122563","DOI":"10.1021\/bi991447r"},{"key":"10.52601\/bpr.2024.240047.swwlxb.11","doi-asserted-by":"publisher","unstructured":"Cornell BA, Middlehurst J, Separovic F (1980) The molecular packing and stability within highly curved phospholipid bilayers. Biochim Biophys Acta 598(2): 405\u2212410","DOI":"10.1016\/0005-2736(80)90018-8"},{"key":"10.52601\/bpr.2024.240047.swwlxb.12","doi-asserted-by":"publisher","unstructured":"Cutler RG, Kelly J, Storie K, Pedersen WA, Tammara A, Hatanpaa K, Troncoso JC, Mattson MP (2004) Involvement of oxidative stress-induced abnormalities in ceramide and cholesterol metabolism in brain aging and Alzheimer's disease. Proc Natl Acad Sci USA 101(7): 2070\u22122075","DOI":"10.1073\/pnas.0305799101"},{"key":"10.52601\/bpr.2024.240047.swwlxb.13","doi-asserted-by":"publisher","unstructured":"de Jesus AJ, Kastelowitz N, Yin H (2013) Changes in lipid density induce membrane curvature. RSC Adv 3(33): 13622\u221213625","DOI":"10.1039\/c3ra42332h"},{"key":"10.52601\/bpr.2024.240047.swwlxb.14","doi-asserted-by":"publisher","unstructured":"Diao J, Burre J, Vivona S, Cipriano DJ, Sharma M, Kyoung M, S\u00fcdhof TC, Brunger AT (2013) Native alpha-synuclein induces clustering of synaptic-vesicle mimics via binding to phospholipids and synaptobrevin-2\/VAMP2. Elife 2: e00592. https:\/\/doi.org\/10.7554\/eLife.00592","DOI":"10.7554\/eLife.00592"},{"key":"10.52601\/bpr.2024.240047.swwlxb.15","doi-asserted-by":"publisher","unstructured":"Eliezer D, Kutluay E, Bussell R Jr, Browne G (2001) Conformational properties of alpha-synuclein in its free and lipid-associated states. J Mol Biol 307(4): 1061\u22121073","DOI":"10.1006\/jmbi.2001.4538"},{"key":"10.52601\/bpr.2024.240047.swwlxb.16","doi-asserted-by":"publisher","unstructured":"Essmann U, Perera L, Berkowitz ML (1995) A smooth particle mesh Ewald method. J Chem Phys 103: 8577\u22128593","DOI":"10.1063\/1.470117"},{"key":"10.52601\/bpr.2024.240047.swwlxb.17","doi-asserted-by":"publisher","unstructured":"Fakhree MA, Zijlstra N, Raiss CC, Siero CJ, Grabmayr H, Bausch AR, Blum C, Claessens MM (2016) The number of \u03b1-synuclein proteins per vesicle gives insights into its physiological function. Sci Rep 6: 30658. https:\/\/doi.org\/10.1038\/srep30658","DOI":"10.1038\/srep30658"},{"key":"10.52601\/bpr.2024.240047.swwlxb.18","doi-asserted-by":"crossref","unstructured":"Fantini J, Yahi N (2013) The driving force of alpha-synuclein insertion and amyloid channel formation in the plasma membrane of neural cells: key role of ganglioside- and cholesterol-binding domains. Adv Exp Med Biol 991: 15\u221226","DOI":"10.1007\/978-94-007-6331-9_2"},{"key":"10.52601\/bpr.2024.240047.swwlxb.19","doi-asserted-by":"publisher","unstructured":"Frank C, Rufini S, Tancredi V, Forcina R, Grossi D, D'Arcangelo G (2008) Cholesterol depletion inhibits synaptic transmission and synaptic plasticity in rat hippocampus. Exp Neurol 212(2): 407\u2212414","DOI":"10.1016\/j.expneurol.2008.04.019"},{"key":"10.52601\/bpr.2024.240047.swwlxb.20","doi-asserted-by":"publisher","unstructured":"Gil C, Soler-Jover A, Blasi J, Aguilera J (2005) Synaptic proteins and SNARE complexes are localized in lipid rafts from rat brain synaptosomes. Biochem Biophys Res Commun 329(1): 117\u2212124","DOI":"10.1016\/j.bbrc.2005.01.111"},{"key":"10.52601\/bpr.2024.240047.swwlxb.21","doi-asserted-by":"publisher","unstructured":"Goedert M (2001) Alpha-synuclein and neurodegenerative diseases. Nat Rev Neurosci 2: 492\u2212501","DOI":"10.1038\/35081564"},{"key":"10.52601\/bpr.2024.240047.swwlxb.22","doi-asserted-by":"publisher","unstructured":"Hess B (2008) P-LINCS: a parallel linear constraint solver for molecular simulation. J Chem Theory Comput 4(1): 116\u2212122","DOI":"10.1021\/ct700200b"},{"key":"10.52601\/bpr.2024.240047.swwlxb.23","doi-asserted-by":"publisher","unstructured":"Huang C, Mason JT (1978) Geometric packing constraints in egg phosphatidylcholine vesicles. Proc Natl Acad Sci USA 75(1): 308\u2212310","DOI":"10.1073\/pnas.75.1.308"},{"key":"10.52601\/bpr.2024.240047.swwlxb.24","doi-asserted-by":"publisher","unstructured":"Humphrey W, Dalke A, Schulten K (1996) VMD: visual molecular dynamics. J Mol Graph 14(1): 33\u221238","DOI":"10.1016\/0263-7855(96)00018-5"},{"key":"10.52601\/bpr.2024.240047.swwlxb.25","doi-asserted-by":"publisher","unstructured":"Jakubec M, Bari\u00e5s E, Furse S, Govasli ML, George V, Turcu D, Iashchishyn IA, Morozova-Roche LA, Halskau \u00d8 (2021) Cholesterol-containing lipid nanodiscs promote an \u03b1-synuclein binding mode that accelerates oligomerization. FEBS J 288(6): 1887\u22121905","DOI":"10.1111\/febs.15551"},{"key":"10.52601\/bpr.2024.240047.swwlxb.26","doi-asserted-by":"publisher","unstructured":"Jensen MB, Bhatia VK, Jao CC, Rasmussen JE, Pedersen SL, Jensen KJ, Langen R, Stamou D (2011) Membrane curvature sensing by amphipathic helices: a single liposome study using \u03b1-synuclein and annexin B12. J Biol Chem 286: 42603\u221242614","DOI":"10.1074\/jbc.M111.271130"},{"key":"10.52601\/bpr.2024.240047.swwlxb.27","doi-asserted-by":"publisher","unstructured":"Jensen PH, Nielsen MS, Jakes R, Dotti CG, Goedert M (1998) Binding of alpha- synuclein to brain vesicles is abolished by familial Parkinson\u2019s disease mutation. J Biol Chem 273: 26292\u221226294","DOI":"10.1074\/jbc.273.41.26292"},{"key":"10.52601\/bpr.2024.240047.swwlxb.28","doi-asserted-by":"publisher","unstructured":"Jorgensen WL, Chandrasekhar J, Madura JD, Impey RW; Klein ML (1983) Comparison of simple potential functions for simulating liquid water. J Chem Phys 79: 926\u2212935","DOI":"10.1063\/1.445869"},{"key":"10.52601\/bpr.2024.240047.swwlxb.29","doi-asserted-by":"publisher","unstructured":"Jo S, Kim T, Iyer VG, Im W (2008) CHARMM-GUI: a web-based graphical user interface for CHARMM. J Comput Chem 29: 1859\u22121865","DOI":"10.1002\/jcc.20945"},{"key":"10.52601\/bpr.2024.240047.swwlxb.30","doi-asserted-by":"publisher","unstructured":"Kang C, Fujioka K, Sun R (2024) Atomistic insight into the lipid nanodomains of synaptic vesicles. J Phys Chem B 128(11): 2707\u22122716","DOI":"10.1021\/acs.jpcb.3c07982"},{"key":"10.52601\/bpr.2024.240047.swwlxb.31","doi-asserted-by":"publisher","unstructured":"Klauda JB, Venable RM, Freites JA, O'Connor JW, Tobias DJ, Mondragon-Ramirez C, Vorobyov I, MacKerell AD Jr, Pastor RW (2010) Update of the CHARMM all-atom additive force field for lipids: validation on six lipid types. J Phys Chem B 114(23): 7830\u22127843","DOI":"10.1021\/jp101759q"},{"key":"10.52601\/bpr.2024.240047.swwlxb.32","doi-asserted-by":"publisher","unstructured":"Kruger R, Kuhn W, Muller T, Woitalla D, Graeber M, K\u00f6sel S, Przuntek H, Epplen JT, Sch\u00f6ls L, Riess O (1998) Ala30Pro mutation in the gene encoding alpha- synuclein in Parkinson\u2019s disease. Nat Genetics 18: 106\u2212108","DOI":"10.1038\/ng0298-106"},{"key":"10.52601\/bpr.2024.240047.swwlxb.33","doi-asserted-by":"publisher","unstructured":"Lai Y, Zhao C, Tian Z, Wang C, Fan J, Hu X, Tu J, Li T, Leitz J, Pfuetzner RA, Liu Z, Zhang S, Su Z, Burr\u00e9 J, Li D, S\u00fcdhof TC, Zhu ZJ, Liu C, Brunger AT, Diao J (2023) Neutral lysophosphatidylcholine mediates \u03b1-synuclein-induced synaptic vesicle clustering. Proc Natl Acad Sci USA 120(44): e2310174120. https:\/\/doi.org\/10.1073\/pnas.2310174120","DOI":"10.1073\/pnas.2310174120"},{"key":"10.52601\/bpr.2024.240047.swwlxb.34","doi-asserted-by":"crossref","unstructured":"Lang T (2007) SNARE proteins and 'membrane rafts'. J Physiol 585(Pt 3): 693-698","DOI":"10.1113\/jphysiol.2007.134346"},{"key":"10.52601\/bpr.2024.240047.swwlxb.35","doi-asserted-by":"crossref","unstructured":"Lautenschlager J, Kaminski CF, Schierle GSK (2017) Alpha-synuclein \u2014 regulator of exocytosis, endocytosis, or both? Trends Cell Biol 27: 468\u2013479","DOI":"10.1016\/j.tcb.2017.02.002"},{"key":"10.52601\/bpr.2024.240047.swwlxb.36","doi-asserted-by":"publisher","unstructured":"Lee J, Cheng X, Swails JM, Yeom MS, Eastman PK, Lemkul JA, Wei S, Buckner J, Jeong JC, Qi Y, Jo S, Pande VS, Case DA, Brooks CL 3rd, MacKerell AD Jr, Klauda JB, Im W (2016) CHARMM-GUI input generator for NAMD, GROMACS, AMBER, OpenMM, and CHARMM\/OpenMM simulations using the CHARMM36 additive force field. J Chem Theory Comput 12(1): 405\u2212413","DOI":"10.1021\/acs.jctc.5b00935"},{"key":"10.52601\/bpr.2024.240047.swwlxb.37","doi-asserted-by":"publisher","unstructured":"Leftin A, Job C, Beyer K, Brown MF (2013) Solid-state \u00b9\u00b3C NMR reveals annealing of raft-like membranes containing cholesterol by the intrinsically disordered protein \u03b1-synuclein. J Mol Biol 425(16): 2973\u22122987","DOI":"10.1016\/j.jmb.2013.04.002"},{"key":"10.52601\/bpr.2024.240047.swwlxb.38","doi-asserted-by":"publisher","unstructured":"Leontiadou H, Mark AE, Marrink SJ (2004) Molecular dynamics simulations of hydrophilic pores in lipid bilayers. Biophys J 86(4): 2156\u22122164","DOI":"10.1016\/S0006-3495(04)74275-7"},{"key":"10.52601\/bpr.2024.240047.swwlxb.39","doi-asserted-by":"crossref","unstructured":"Liu J, Bu B, Crowe M, Li D, Diao J, Ji B (2021) Membrane packing defects in synaptic vesicles recruit complexin and synuclein. Phys Chem Chem Phys 23(3): 2117\u22122125","DOI":"10.1039\/D0CP03546G"},{"key":"10.52601\/bpr.2024.240047.swwlxb.40","doi-asserted-by":"publisher","unstructured":"Liu S, Ninan I, Antonova I, Battaglia F, Trinchese F, Narasanna A, Kolodilov N, Dauer W, Hawkins RD, Arancio O (2004) alpha-synuclein produces a long-lasting increase in neurotransmitter release. EMBO J 23(22): 4506\u22124516","DOI":"10.1038\/sj.emboj.7600451"},{"key":"10.52601\/bpr.2024.240047.swwlxb.41","doi-asserted-by":"publisher","unstructured":"Lokappa SB, Ulmer TS (2011) Alpha-synuclein populates both elongated and broken helix states on small unilamellar vesicles. J Biol Chem 286(24): 21450\u221221457","DOI":"10.1074\/jbc.M111.224055"},{"key":"10.52601\/bpr.2024.240047.swwlxb.42","doi-asserted-by":"publisher","unstructured":"Mahapatra A, Mandal N, Chattopadhyay K (2021) Cholesterol in synaptic vesicle membranes regulates the vesicle-binding, function, and aggregation of \u03b1-synuclein. J Phys Chem B 125(40): 11099\u221211111","DOI":"10.1021\/acs.jpcb.1c03533"},{"key":"10.52601\/bpr.2024.240047.swwlxb.43","doi-asserted-by":"publisher","unstructured":"Manna M, Murarka RK (2021) Polyunsaturated fatty acid modulates membrane-bound monomeric \u03b1-synuclein by modulating membrane microenvironment through preferential interactions. ACS Chem Neurosci 12(4): 675\u2212688","DOI":"10.1021\/acschemneuro.0c00694"},{"key":"10.52601\/bpr.2024.240047.swwlxb.44","doi-asserted-by":"publisher","unstructured":"Man WK, De Simone A, Barritt JD, Vendruscolo M, Dobson CM, Fusco G (2020) A role of cholesterol in modulating the binding of \u03b1-synuclein to synaptic-like vesicles. Front Neurosci 14: 18. https:\/\/doi.org\/10.3389\/fnins.2020.00018","DOI":"10.3389\/fnins.2020.00018"},{"key":"10.52601\/bpr.2024.240047.swwlxb.45","doi-asserted-by":"publisher","unstructured":"Maroteaux L, Campanelli JT, Scheller RH (1988) Synuclein \u2014 a neuron-specific protein localized to the nucleus and presynaptic nerve-terminal. J Neurosci 8: 2804\u22122815","DOI":"10.1523\/JNEUROSCI.08-08-02804.1988"},{"key":"10.52601\/bpr.2024.240047.swwlxb.46","doi-asserted-by":"publisher","unstructured":"McClain SM, Milchberg MH, Rienstra CM, Murphy CJ (2023) Biologically representative lipid-coated gold nanoparticles and phospholipid vesicles for the study of alpha-synuclein\/membrane interactions. ACS Nano 17(20): 20387\u221220401","DOI":"10.1021\/acsnano.3c06606"},{"key":"10.52601\/bpr.2024.240047.swwlxb.47","doi-asserted-by":"publisher","unstructured":"Middleton ER, Rhoades E (2010) Effects of curvature and composition on alpha-synuclein binding to lipid vesicles. Biophys J 99: 2279\u22122288","DOI":"10.1016\/j.bpj.2010.07.056"},{"key":"10.52601\/bpr.2024.240047.swwlxb.48","doi-asserted-by":"publisher","unstructured":"Necula M, Chirita CN, Kuret J (2003) Rapid anionic micelle-mediated alpha-synuclein fibrillization <i>in vitro<\/i>. J Biol Chem 278: 46674\u221246680","DOI":"10.1074\/jbc.M308231200"},{"key":"10.52601\/bpr.2024.240047.swwlxb.49","doi-asserted-by":"publisher","unstructured":"Nuscher B, Kamp F, Mehnert T, Odoy S, Haass C, Kahle PJ, Beyer K (2004) Alpha-synuclein has a high affinity for packing defects in a bilayer membrane: a thermodynamics study. J Biol Chem 279(21): 21966\u221221975","DOI":"10.1074\/jbc.M401076200"},{"key":"10.52601\/bpr.2024.240047.swwlxb.50","doi-asserted-by":"publisher","unstructured":"Parrinello M, Rahman A (1981) Polymorphic transitions in single-crystals \u2014 a new molecular-dynamics method. J Appl Phys 52: 7182\u22127190","DOI":"10.1063\/1.328693"},{"key":"10.52601\/bpr.2024.240047.swwlxb.51","doi-asserted-by":"publisher","unstructured":"Perlmutter JD Braun AR, Sachs JN (2009) Curvature dynamics of alpha-synuclein familial Parkinson disease mutants: molecular simulations of the micelle- and bilayer-bound forms. J Biol Chem 284: 7177\u22127189","DOI":"10.1074\/jbc.M808895200"},{"key":"10.52601\/bpr.2024.240047.swwlxb.52","doi-asserted-by":"publisher","unstructured":"Qi Z, Wan M, Zhang J, Li Z (2023) Influence of cholesterol on the membrane binding and conformation of \u03b1-synuclein. J Phys Chem B 127(9): 1956\u22121964","DOI":"10.1021\/acs.jpcb.2c08077"},{"key":"10.52601\/bpr.2024.240047.swwlxb.53","doi-asserted-by":"publisher","unstructured":"Serpell LC, Berriman J, Jakes R, Goedert M, Crowther RA (2000) Fiber diffraction of synthetic alpha- synuclein filaments shows amyloid- like cross- beta conformation. Proc Natl Acad Sci USA 97: 4897\u22124902","DOI":"10.1073\/pnas.97.9.4897"},{"key":"10.52601\/bpr.2024.240047.swwlxb.54","doi-asserted-by":"publisher","unstructured":"Spillantini MG, Goedert M (2000) The alpha-synucleinopathies: Parkinson's disease, dementia with Lewy bodies, and multiple system atrophy. Ann N Y Acad Sci 920: 16\u221227","DOI":"10.1111\/j.1749-6632.2000.tb06900.x"},{"key":"10.52601\/bpr.2024.240047.swwlxb.55","doi-asserted-by":"publisher","unstructured":"Stefanovic AN, Claessens MM, Blum C, Subramaniam V (2015) Alpha-synuclein amyloid oligomers act as multivalent nanoparticles to cause hemifusion in negatively charged vesicles. Small 11(19): 2257\u22122262","DOI":"10.1002\/smll.201402674"},{"key":"10.52601\/bpr.2024.240047.swwlxb.56","doi-asserted-by":"publisher","unstructured":"Tang Z, Fang Z, Wu X, Liu J, Tian L, Li X, Diao J, Ji B, Li D (2024) Folding of N-terminally acetylated \u03b1-synuclein upon interaction with lipid membranes. Biophys J 123(21): 3698\u22123720","DOI":"10.1016\/j.bpj.2024.09.019"},{"key":"10.52601\/bpr.2024.240047.swwlxb.57","doi-asserted-by":"publisher","unstructured":"Tsigelny IF, Sharikov Y, Kouznetsova VL, Greenberg JP, Wrasidlo W, Overk C, Gonzalez T, Trejo M, Spencer B, Kosberg K, Masliah E (2015) Molecular determinants of \u03b1-synuclein mutants' oligomerization and membrane interactions. ACS Chem Neurosci 6(3): 403\u2212416","DOI":"10.1021\/cn500332w"},{"key":"10.52601\/bpr.2024.240047.swwlxb.58","doi-asserted-by":"publisher","unstructured":"Tyoe O, Aryal C, Diao J (2023) Docosahexaenoic acid promotes vesicle clustering mediated by alpha-synuclein via electrostatic interaction. Eur Phys J E Soft Matter 46(10): 96. https:\/\/doi.org\/10.1140\/epje\/s10189-023-00353-z","DOI":"10.1140\/epje\/s10189-023-00353-z"},{"key":"10.52601\/bpr.2024.240047.swwlxb.59","doi-asserted-by":"publisher","unstructured":"Ulmer TS, Bax A, Cole NB, Nussbaum RL (2005) Structure and dynamics of micelle-bound human alpha-synuclein. J Biol Chem 280(10): 9595\u22129603","DOI":"10.1074\/jbc.M411805200"},{"key":"10.52601\/bpr.2024.240047.swwlxb.60","doi-asserted-by":"publisher","unstructured":"Valenza M, Carroll JB, Leoni V, Bertram LN, Bj\u00f6rkhem I, Singaraja RR, Di Donato S, Lutjohann D, Hayden MR, Cattaneo E (2007) Cholesterol biosynthesis pathway is disturbed in YAC128 mice and is modulated by huntingtin mutation. Hum Mol Genet 16(18): 2187\u22122198","DOI":"10.1093\/hmg\/ddm170"},{"key":"10.52601\/bpr.2024.240047.swwlxb.61","doi-asserted-by":"publisher","unstructured":"Vamparys L, Gautier R, Vanni S, Bennett WF, Tieleman DP, Antonny B, Etchebest C, Fuchs PF (2013) Conical lipids in flat bilayers induce packing defects similar to that induced by positive curvature. Biophys J 104(3): 585\u2212593","DOI":"10.1016\/j.bpj.2012.11.3836"},{"key":"10.52601\/bpr.2024.240047.swwlxb.62","doi-asserted-by":"publisher","unstructured":"van den Brink-van der Laan E, Killian JA, de Kruijff B (2004) Nonbilayer lipids affect peripheral and integral membrane proteins via changes in the lateral pressure profile. Biochim Biophys Acta 1666(1-2): 275\u221288","DOI":"10.1016\/j.bbamem.2004.06.010"},{"key":"10.52601\/bpr.2024.240047.swwlxb.63","doi-asserted-by":"publisher","unstructured":"Van Der Spoel D, Lindahl E, Hess B, Groenhof G, Mark AE, Berendsen HJ (2005) GROMACS: fast, flexible, and free. J Comput Chem 26(16): 1701\u22121718","DOI":"10.1002\/jcc.20291"},{"key":"10.52601\/bpr.2024.240047.swwlxb.64","doi-asserted-by":"crossref","unstructured":"van Maarschalkerweerd A, Vetri V, Vestergaard B (2015) Cholesterol facilitates interactions between \u03b1-synuclein oligomers and charge-neutral membranes. FEBS Lett 589(19 Pt B): 2661\u22122667","DOI":"10.1016\/j.febslet.2015.08.013"},{"key":"10.52601\/bpr.2024.240047.swwlxb.65","doi-asserted-by":"publisher","unstructured":"Vermaas JV, Tajkhorshid E (2014) Conformational heterogeneity of \u03b1-synuclein in membrane. Biochim Biophys Acta 1838(12): 3107\u22123117","DOI":"10.1016\/j.bbamem.2014.08.012"},{"key":"10.52601\/bpr.2024.240047.swwlxb.66","doi-asserted-by":"publisher","unstructured":"Wang C, Zhao C, Li D, Tian Z, Lai Y, Diao J, Liu C (2016) Versatile structures of alpha-synuclein. Front Mol Neurosci 9: 48. https:\/\/doi.org\/10.3389\/fnmol.2016.00048","DOI":"10.3389\/fnmol.2016.00048"},{"key":"10.52601\/bpr.2024.240047.swwlxb.67","doi-asserted-by":"publisher","unstructured":"Wu K, Li D, Xiu P, Ji B, Diao J (2020) O-GlcNAcylation inhibits the oligomerization of alpha-synuclein by declining intermolecular hydrogen bonds through a steric effect. Phys Biol 18(1): 016002. https:\/\/doi.org\/10.1088\/1478-3975\/abb6dc","DOI":"10.1088\/1478-3975\/abb6dc"},{"key":"10.52601\/bpr.2024.240047.swwlxb.68","doi-asserted-by":"publisher","unstructured":"Zhu M, Li J, Fink AL (2003) The association of alpha- synuclein with membranes affects bilayer structure, stability, and fibril formation. J Biol Chem 278: 40186\u221240197","DOI":"10.1074\/jbc.M305326200"}],"container-title":["Biophysics Reports"],"original-title":[],"deposited":{"date-parts":[[2025,12,24]],"date-time":"2025-12-24T06:56:45Z","timestamp":1766559405000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.biophysics-reports.org\/article\/doi\/10.52601\/bpr.2024.240047"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025]]},"references-count":68,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2025]]}},"alternative-id":["2024-047-DJJ"],"URL":"https:\/\/doi.org\/10.52601\/bpr.2024.240047","relation":{},"ISSN":["2364-3439","2364-3420"],"issn-type":[{"value":"2364-3439","type":"print"},{"value":"2364-3420","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025]]}}}