{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,22]],"date-time":"2026-06-22T08:49:17Z","timestamp":1782118157972,"version":"3.54.5"},"reference-count":22,"publisher":"Biophysical Society of China","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["swwlxb"],"published-print":{"date-parts":[[2026]]},"DOI":"10.52601\/bpr.2025.250014","type":"journal-article","created":{"date-parts":[[2025,4,11]],"date-time":"2025-04-11T03:04:06Z","timestamp":1744340646000},"page":"137","source":"Crossref","is-referenced-by-count":1,"title":["Enrichment and amplification of secreted \u03b1-synuclein from cellular model and saliva of patients with synucleinopathies"],"prefix":"10.52601","volume":"12","author":[{"given":"Wuxian","family":"Xu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mingyue","family":"Luan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhaoxia","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianwen","family":"Deng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li","family":"Zhu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"30428","reference":[{"key":"10.52601\/bpr.2025.250014.swwlxb.1","doi-asserted-by":"publisher","unstructured":"Bargar C, Wang W, Gunzler SA, LeFevre A, Wang Z, Lerner AJ, Singh N, Tatsuoka C, Appleby B, Zhu X, Xu R, Haroutunian V, Zou W-Q, Ma J, Chen SG (2021) Streamlined alpha-synuclein RT-QuIC assay for various biospecimens in Parkinson\u2019s disease and dementia with Lewy bodies. Acta Neuropathol Commun 9(1): 62. https:\/\/doi.org\/10.1186\/s40478-021-01175-w","DOI":"10.1186\/s40478-021-01175-w"},{"key":"10.52601\/bpr.2025.250014.swwlxb.2","doi-asserted-by":"publisher","unstructured":"Calabresi P, Mechelli A, Natale G, Volpicelli-Daley L, Di Lazzaro G, Ghiglieri V (2023) Alpha-synuclein in Parkinson\u2019s disease and other synucleinopathies: from overt neurodegeneration back to early synaptic dysfunction. Cell Death Dis 14(3): 176. https:\/\/doi.org\/10.1038\/s41419-023-05672-9","DOI":"10.1038\/s41419-023-05672-9"},{"key":"10.52601\/bpr.2025.250014.swwlxb.3","doi-asserted-by":"publisher","unstructured":"Calo L, Wegrzynowicz M, Santiva\u00f1ez\u2010Perez J, Grazia Spillantini M (2016) Synaptic failure and \u03b1\u2010synuclein. Mov Disord 31(2): 169\u2212177","DOI":"10.1002\/mds.26479"},{"key":"10.52601\/bpr.2025.250014.swwlxb.4","doi-asserted-by":"publisher","unstructured":"Concha-Marambio L, Pritzkow S, Shahnawaz M, Farris CM, Soto C (2023) Seed amplification assay for the detection of pathologic alpha-synuclein aggregates in cerebrospinal fluid. Nat Protoc 18(4): 1179\u22121196","DOI":"10.1038\/s41596-022-00787-3"},{"key":"10.52601\/bpr.2025.250014.swwlxb.5","doi-asserted-by":"publisher","unstructured":"Du T, Wang L, Liu W, Zhu G, Chen Y, Zhang J (2021) Biomarkers and the role of \u03b1-synuclein in Parkinson\u2019s disease. Front Aging Neurosci 13: 645996. https:\/\/doi.org\/10.3389\/fnagi.2021.645996","DOI":"10.3389\/fnagi.2021.645996"},{"key":"10.52601\/bpr.2025.250014.swwlxb.6","doi-asserted-by":"crossref","unstructured":"Ghosh D, Mehra S, Sahay S, Singh PK, Maji SK (2017) \u03b1-synuclein aggregation and its modulation. Int J Biol Macromol 100(2017): 37\u221254","DOI":"10.1016\/j.ijbiomac.2016.10.021"},{"key":"10.52601\/bpr.2025.250014.swwlxb.7","doi-asserted-by":"publisher","unstructured":"Groveman BR, Orr\u00f9 CD, Hughson AG, Raymond LD, Zanusso G, Ghetti B, Campbell KJ, Safar J, Galasko D, Caughey B (2018) Rapid and ultra-sensitive quantitation of disease-associated \u03b1-synuclein seeds in brain and cerebrospinal fluid by \u03b1Syn RT-QuIC. Acta Neuropathol Commun 6(1): 7. https:\/\/doi.org\/10.1186\/s40478-018-0508-2","DOI":"10.1186\/s40478-018-0508-2"},{"key":"10.52601\/bpr.2025.250014.swwlxb.8","doi-asserted-by":"publisher","unstructured":"Hong Z, Shi M, Chung KA, Quinn JF, Peskind ER, Galasko D, Jankovic J, Zabetian CP, Leverenz JB, Baird G, Montine TJ, Hancock AM, Hwang H, Pan C, Bradner J, Kang UJ, Jensen PH, Zhang J (2010) DJ-1 and \u03b1-synuclein in human cerebrospinal fluid as biomarkers of Parkinson\u2019s disease. Brain 133(3): 713\u2212726","DOI":"10.1093\/brain\/awq008"},{"key":"10.52601\/bpr.2025.250014.swwlxb.9","doi-asserted-by":"publisher","unstructured":"Long H, Zeng S, Li D (2022a) Cellular and animal models to investigate pathogenesis of amyloid aggregation in neurodegenerative diseases. Biophy Rep 8(1): 14\u221228","DOI":"10.52601\/bpr.2022.210033"},{"key":"10.52601\/bpr.2025.250014.swwlxb.10","doi-asserted-by":"publisher","unstructured":"Long H, Zeng S, Sun Y, Liu C (2022b) Biochemical and biophysical characterization of pathological aggregation of amyloid proteins. Biophys Rep 8(1): 42\u221254","DOI":"10.52601\/bpr.2022.210032"},{"key":"10.52601\/bpr.2025.250014.swwlxb.11","doi-asserted-by":"publisher","unstructured":"Huang C, Ren G, Zhou H, Wang C-C (2005) A new method for purification of recombinant human \u03b1-synuclein in <i>Escherichia coli<\/i>. Protein Expr Purif 42(1): 173\u2212177","DOI":"10.1016\/j.pep.2005.02.014"},{"key":"10.52601\/bpr.2025.250014.swwlxb.12","doi-asserted-by":"publisher","unstructured":"Iraci N, Marchetti B, Giachino C, Falzone GG, Vivarelli S, Patern\u00f2 G, Leggio L (2021) Extracellular vesicles as novel diagnostic and prognostic biomarkers for Parkinson\u2019s disease. Aging Dis 12(6): 1494\u22121515","DOI":"10.14336\/AD.2021.0527"},{"key":"10.52601\/bpr.2025.250014.swwlxb.13","doi-asserted-by":"publisher","unstructured":"Khalaf O, Fauvet B, Oueslati A, Dikiy I, Mahul-Mellier A-L, Ruggeri FS, Mbefo MK, Vercruysse F, Dietler G, Lee S-J, Eliezer D, Lashuel HA (2014) The H50Q mutation enhances \u03b1-synuclein aggregation, secretion, and toxicity. J Biol Chem 289(32): 21856\u221221876","DOI":"10.1074\/jbc.M114.553297"},{"key":"10.52601\/bpr.2025.250014.swwlxb.14","doi-asserted-by":"publisher","unstructured":"Kuzkina A, Bargar C, Schmitt D, R\u00f6\u00dfle J, Wang W, Schubert A-L, Tatsuoka C, Gunzler SA, Zou W-Q, Volkmann J, Sommer C, Doppler K, Chen SG (2021) Diagnostic value of skin RT-QuIC in Parkinson\u2019s disease: a two-laboratory study. NPJ Parkinson's Dis 7(1): 99\u2212110","DOI":"10.1038\/s41531-021-00242-2"},{"key":"10.52601\/bpr.2025.250014.swwlxb.15","doi-asserted-by":"publisher","unstructured":"Luan M, Sun Y, Chen J, Jiang Y, Li F, Wei L, Sun W, Ma J, Song L, Liu J, Liu B, Pei Y, Wang Z, Zhu L, Deng J (2022) Diagnostic value of salivary real\u2010time quaking\u2010induced conversion in Parkinson's disease and multiple system atrophy. Mov Disord 37(5): 1059\u22121063","DOI":"10.1002\/mds.28976"},{"key":"10.52601\/bpr.2025.250014.swwlxb.16","doi-asserted-by":"publisher","unstructured":"Luan M, Wei L, Sun Y, Chen J, Jiang Y, Wu W, Li F, Sun W, Zhu L, Wang Z, Deng J (2024) Combining salivary \u03b1-synuclein seeding activity and miRNA-29a to distinguish Parkinson's disease and multiple system atrophy. Parkinsonism Relat Disord 127(2024): 107088. https:\/\/doi.org\/10.1016\/j.parkreldis.2024.107088","DOI":"10.1016\/j.parkreldis.2024.107088"},{"key":"10.52601\/bpr.2025.250014.swwlxb.17","doi-asserted-by":"publisher","unstructured":"Peng H, Chen S, Wu S, Shi X, Ma J, Yang H, Li X (2023) Alpha-synuclein in skin as a high-quality biomarker for Parkinson's disease. J Neurol Sci 451(2023): 120730. https:\/\/doi.org\/10.1016\/j.jns.2023.120730","DOI":"10.1016\/j.jns.2023.120730"},{"key":"10.52601\/bpr.2025.250014.swwlxb.18","doi-asserted-by":"publisher","unstructured":"Shaheen H, Sobhy S, El Mously S, Abuomira M, Mansour M (2020) Salivary alpha-synuclein (total and oligomeric form): potential biomarkers in Parkinson\u2019s disease. Egypt J Neurol Psychiatry Neurosurg 56(1): 22 https:\/\/doi.org\/10.1186\/s41983-020-0159-7","DOI":"10.1186\/s41983-020-0159-7"},{"key":"10.52601\/bpr.2025.250014.swwlxb.19","doi-asserted-by":"publisher","unstructured":"Srivastava A, Alam P, Caughey B (2022) RT-QuIC and related assays for detecting and quantifying prion-like pathological seeds of \u03b1-synuclein. Biomolecules 12(4): 576. https:\/\/doi.org\/10.3390\/biom12040576","DOI":"10.3390\/biom12040576"},{"key":"10.52601\/bpr.2025.250014.swwlxb.20","doi-asserted-by":"publisher","unstructured":"Van der Perren A, Gelders G, Fenyi A, Bousset L, Brito F, Peelaerts W, Van den Haute C, Gentleman S, Melki R, Baekelandt V (2020) The structural differences between patient-derived \u03b1-synuclein strains dictate characteristics of Parkinson\u2019s disease, multiple system atrophy and dementia with Lewy bodies. Acta Neuropathol 139(6): 977\u22121000","DOI":"10.1007\/s00401-020-02157-3"},{"key":"10.52601\/bpr.2025.250014.swwlxb.21","doi-asserted-by":"publisher","unstructured":"Xicoy H, Wieringa B, Martens GJM (2017) The SH-SY5Y cell line in Parkinson\u2019s disease research: a systematic review. Mol Neurodegeneration 12(1): 10. https:\/\/doi.org\/10.1186\/s13024-017-0149-0","DOI":"10.1186\/s13024-017-0149-0"},{"key":"10.52601\/bpr.2025.250014.swwlxb.22","doi-asserted-by":"publisher","unstructured":"Yuan X, Nie S, Yang Y, Liu C, Xia D, Meng L, Xia Y, Su H, Zhang C, Bu L, Deng M, Ye K, Xiong J, Chen L, Zhang Z (2025) Propagation of pathologic \u03b1-synuclein from kidney to brain may contribute to Parkinson\u2019s disease. Nat Neurosci 28(3): 577\u2212588","DOI":"10.1038\/s41593-024-01866-2"}],"container-title":["Biophysics Reports"],"original-title":[],"deposited":{"date-parts":[[2026,6,22]],"date-time":"2026-06-22T08:11:50Z","timestamp":1782115910000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.biophysics-reports.org\/article\/doi\/10.52601\/bpr.2025.250014"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026]]},"references-count":22,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2026]]}},"alternative-id":["2025-014-ZL"],"URL":"https:\/\/doi.org\/10.52601\/bpr.2025.250014","relation":{},"ISSN":["2364-3439","2364-3420"],"issn-type":[{"value":"2364-3439","type":"print"},{"value":"2364-3420","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026]]}}}