{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T22:50:06Z","timestamp":1792104606542,"version":"4.4.0"},"reference-count":0,"publisher":"Universa BV","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Acta Orthop Belg"],"abstract":"<jats:p>This study evaluated whether three-dimensional (3D) bioprinted spherical, porous poly-L-lactic acid (PLLA) drug- loaded implants, combined with bone marrow mesenchymal stem cells (BMSCs) overexpressing transforming growth factor-\u03b21 (TGF-\u03b21) and interleukin-10 (IL-10), can enhance the repair of osteoarthritis (OA)-related cartilage defects. Rat BMSCs were transduced with lentiviral vectors to achieve dual overexpression of TGF-\u03b21 and IL-10, induced toward chondrogenic differentiation, and assessed for chondrogenic marker expression (COL2A1, SOX-9, and aggrecan). Spherical porous PLLA drug-loaded implants were fabricated by 3D bioprinting and characterized for drug loading, porosity, cytocompatibility, skin sensitization, biodegradation, and in vivo release behavior. Histology, micro-computed tomography (micro-CT), and molecular assays evaluated cartilage repair and subchondral bone remodeling. Dual-gene transduction markedly promoted BMSC chondrogenic differentiation with significant up-regulation of COL2A1, SOX-9, and aggrecan. The PLLA scaffolds exhibited high porosity (88.88 \u00b1 2.87%), meeting biomaterial requirements, showed no evident cytotoxicity or skin sensitization, and achieved a 12-week mass-loss (biodegradation) of 42.65%. In vivo, the combined treatment group displayed superior cartilage regeneration and subchondral bone repair compared with controls, accompanied by increased BMP2 and type II collagen (COL-2) expression. 3D-bioprinted porous PLLA drug-loaded implants combined with TGF-\u03b21\/IL-10 dual-gene-transduced BMSCs effectively promote repair of OA cartilage defects, indicating satisfactory biocompatibility and potential for clinical translation.<\/jats:p>","DOI":"10.52628\/92.3.15544","type":"journal-article","created":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T22:37:13Z","timestamp":1792103833000},"source":"Crossref","is-referenced-by-count":0,"title":["3D bioprinted porous PLLA implants with dual gene modified BMSCs for osteoarthritis cartilage repair"],"prefix":"10.52628","volume":"92","author":[{"given":"T.","family":"LIU","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"ZHANG","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B.","family":"WANG","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Z.","family":"MEI","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G.","family":"XIAO","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H.","family":"HE","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Y.","family":"ZHAO","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.-Y.","family":"WU","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Z.","family":"YANG","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K.","family":"DU","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P.","family":"LIU","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"27062","published-online":{"date-parts":[[2026,10]]},"container-title":["Acta Orthopaedica Belgica"],"original-title":[],"deposited":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T22:37:20Z","timestamp":1792103840000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.actaorthopaedica.be\/archive\/volume-92\/autumn\/experimental-basic-science-research\/3d-bioprinted-porous-plla-implants-with-dual-gene-modified-bmscs-for-osteoarthritis-cartil\/"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,10]]},"references-count":0,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2026,10]]}},"URL":"https:\/\/doi.org\/10.52628\/92.3.15544","relation":{},"ISSN":["0001-6462"],"issn-type":[{"value":"0001-6462","type":"print"}],"subject":[],"published":{"date-parts":[[2026,10]]}}}