{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T17:44:11Z","timestamp":1785433451930,"version":"3.56.0"},"reference-count":29,"publisher":"Open Medical Publishing","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"abstract":"\n Background<\/jats:title>\n Osteoarthritis (OA) is a degenerative joint disorder that causes pain, stiffness, and functional impairment. Conventional treatments relieve symptoms but do not restore cartilage, limiting long-term efficacy. Cell-based therapies, including mesenchymal stem cells (MSCs) and MSC-derived secretome, have emerged as promising strategies for cartilage regeneration.<\/jats:p>\n <\/jats:sec>\n \n Object<\/jats:title>\n This study aimed to assess the therapeutic effects of UC-MSCs and UC-MSC-derived secretome in OA patients through both clinical outcomes and synovial fluid (SF) analyses.<\/jats:p>\n <\/jats:sec>\n \n Methods<\/jats:title>\n Eligible participants were divided into two groups; group who underwent arthroscopy and group who did not. All the participants received an intra-articular injection consisting of an initial 2 mL dose of UC-MSC secretome, followed by 10 million UC-MSCs, and two additional 2 mL doses of secretome administered biweekly. Synovial fluid samples were collected at baseline and 12 weeks post-treatment, centrifuged to obtain the supernatant, and analyzed for inflammatory cytokines and matrix-degrading markers using multiplex and ELISA assays. Clinical evaluations were conducted at 6- and 12-months post-treatment.<\/jats:p>\n <\/jats:sec>\n \n Result<\/jats:title>\n The results showed that UC-MSC therapy significantly improved functional outcomes in patients with knee osteoarthritis, as indicated by WOMAC scores up to six months. In vitro studies showed similar results, where co-culture of osteoarthritic synovial fluid-derived MSCs with UC-MSCs or UC-MSC secretome enhanced proliferation and differentiation while rapidly reducing pro-inflammatory cytokines (IL-1\u03b2, IFN-\u03b3, IL-6, IL-12p70, IL-17A, IL-18) and MMPs (MMP1, MMP7, MMP13).<\/jats:p>\n <\/jats:sec>\n \n Conclusion<\/jats:title>\n Our findings support a two-stage therapeutic strategy in which UC-MSC secretome first alleviates inflammation, followed by UC-MSCs to promote cartilage regeneration. Post-injection rehabilitation or repeated MSC dosing may further enhance treatment efficacy, highlighting the potential of MSC-based therapies for knee OA management.<\/jats:p>\n <\/jats:sec>","DOI":"10.52965\/001c.158292","type":"journal-article","created":{"date-parts":[[2026,3,13]],"date-time":"2026-03-13T00:11:10Z","timestamp":1773360670000},"source":"Crossref","is-referenced-by-count":0,"title":["Influence of Synovial Fluid Biochemical Markers on Clinical Outcomes Following Arthroscopic and Non-Arthroscopic Patients with Knee Osteoarthritis"],"prefix":"10.52965","volume":"18","author":[{"family":"Yanuarso","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/0359vrr67","id-type":"ROR","asserted-by":"publisher"}],"name":"Gatot Soebroto Army Hospital","department":["Orthopedic Surgeon"]},{"id":[{"id":"https:\/\/ror.org\/00xqf8t64","id-type":"ROR","asserted-by":"publisher"}],"name":"Universitas Padjadjaran","department":["Departement of Pharmacology and Clinical Pharmacy, Faculty of Pharmacy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ditta Kalyani","family":"Devi","sequence":"additional","affiliation":[{"name":"Prodia StemCell Indonesia, Central Jakarta, Daerah Khusus Ibukota Jakarta 10430, Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Keri","family":"Lestari","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/00xqf8t64","id-type":"ROR","asserted-by":"publisher"}],"name":"Universitas Padjadjaran","department":["Departement of Pharmacology and Clinical Pharmacy, Faculty of Pharmacy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7323-8691","authenticated-orcid":false,"given":"Cynthia Retna","family":"Sartika","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/00xqf8t64","id-type":"ROR","asserted-by":"publisher"}],"name":"Universitas Padjadjaran","department":["Departement of Pharmacology and Clinical Pharmacy, Faculty of Pharmacy"]},{"name":"Prodia StemCell Indonesia, Central Jakarta, Daerah Khusus Ibukota Jakarta 10430, Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-4289-2608","authenticated-orcid":false,"given":"Karina","family":"Kalasuba","sequence":"additional","affiliation":[{"name":"Prodia StemCell Indonesia, Central Jakarta, Daerah Khusus Ibukota Jakarta 10430, Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7806-7078","authenticated-orcid":false,"given":"Rima","family":"Haifa","sequence":"additional","affiliation":[{"name":"Prodia StemCell Indonesia, Central Jakarta, Daerah Khusus Ibukota Jakarta 10430, Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lestari Mahardika","family":"Urbaningrum","sequence":"additional","affiliation":[{"name":"Prodia StemCell Indonesia, Central Jakarta, Daerah Khusus Ibukota Jakarta 10430, Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"30777","published-online":{"date-parts":[[2026,3,13]]},"reference":[{"issue":"1","key":"ref1","doi-asserted-by":"publisher","first-page":"29","DOI":"10.1016\/j.bbcan.2011.10.001","article-title":"New facets of matrix metalloproteinases MMP-2 and MMP-9 as cell surface transducers: outside-in signaling and relationship to tumor progression","volume":"1825","author":"B. 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