{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T19:58:57Z","timestamp":1777579137632,"version":"3.51.4"},"reference-count":25,"publisher":"SAGE Publications","issue":"6","license":[{"start":{"date-parts":[[2012,4,5]],"date-time":"2012-04-05T00:00:00Z","timestamp":1333584000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["European Journal of Ophthalmology"],"published-print":{"date-parts":[[2012,11]]},"abstract":"<jats:sec>\n                    <jats:title>Purpose.<\/jats:title>\n                    <jats:p>Recent investigations suggested that unaffected carriers of Leber hereditary optic neuropathy (LHON) may show subclinical visual alterations. Structural changes have also been detected by optical coherence tomography (OCT), which revealed a temporal thickening of the retinal nerve fiber layer (RNFL). These changes may reflect compensatory effects such as mitochondria accumulation within the RNFL axons. This study aimed to investigate whether the RNFL of LHON carriers shows greater than expected thickness variations, which may reflect transient subclinical changes, over the course of years.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods.<\/jats:title>\n                    <jats:p>Using Stratus OCT, the RNFL thickness was measured yearly from 2005 to 2008 in 24 Brazilian LHON carriers, all with homoplasmic 11778\/ND4 mtDNA mutation. An Italian sample of 20 healthy subjects served as a control. Data were compared also to a previously published sample (n=59) of glaucomatous eyes.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results.<\/jats:title>\n                    <jats:p>The LHON carriers showed test-retest standard deviations that were larger than normal controls in the temporal (p=0.004), superior (p&lt;0.0001), and inferior quadrants (p=0.019). Compared to the glaucoma cases, no statistical differences were observed.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions.<\/jats:title>\n                    <jats:p>The RNFL thickness in LHON carriers, when measured at different time points, has higher variability than in normal subjects. Transitory RNFL swelling may be caused either by compensatory mechanisms (increased mitochondrial biogenesis) or by axonal stasis preceding decompensation of retinal ganglion cells. In both situations, these changes may represent the origin of the visual alterations previously detected in LHON carriers. Alternatively, increased variability of RNFL thickness may be influenced by the LHON microangiopathy, as retinal blood vessels contribute to the OCT RNFL thickness measurements.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.5301\/ejo.5000154","type":"journal-article","created":{"date-parts":[[2012,5,4]],"date-time":"2012-05-04T08:45:51Z","timestamp":1336121151000},"page":"985-991","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":37,"title":["Retinal Nerve Fiber Layer Thickness Variability in Leber Hereditary Optic Neuropathy Carriers"],"prefix":"10.1177","volume":"22","author":[{"given":"Piero","family":"Barboni","sequence":"first","affiliation":[{"name":"Studio Oculistico d'Azeglio, Bologna - Italy"},{"name":"Dipartimento di Scienze Neurologiche, Universit\u00e0 di Bologna, Bologna - Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Giacomo","family":"Savini","sequence":"additional","affiliation":[{"name":"Fondazione G.B. Bietti-IRCCS, Rome - Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"William J.","family":"Feuer","sequence":"additional","affiliation":[{"name":"Bascom Palmer Eye Institute, Department of Ophthalmology, University of Miami Miller School of Medicine, Miami, FL - USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Donald L.","family":"Budenz","sequence":"additional","affiliation":[{"name":"Bascom Palmer Eye Institute, Department of Ophthalmology, University of Miami Miller School of Medicine, Miami, FL - USA"},{"name":"Department of Ophthalmology, University of North Carolina, Chapel Hill, NC - USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michele","family":"Carbonelli","sequence":"additional","affiliation":[{"name":"Studio Oculistico d'Azeglio, Bologna - Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Filipe","family":"Chicani","sequence":"additional","affiliation":[{"name":"Doheny Eye Institute and Department of Ophthalmology, Keck School of Medicine of the University of the Southern California, Los Angeles, CA - USA"},{"name":"Department of Ophthalmology, Federal University of S\u00e3o Paulo, UNIFESP, S\u00e3o Paulo - Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carolina Do V.F.","family":"Ramos","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, Federal University of S\u00e3o Paulo, UNIFESP, S\u00e3o Paulo - Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Solange R.","family":"Salomao","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, Federal University of S\u00e3o Paulo, UNIFESP, S\u00e3o Paulo - Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Annamaria","family":"De Negri","sequence":"additional","affiliation":[{"name":"Azienda San Camillo-Forlanini, Rome - Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vincenzo","family":"Parisi","sequence":"additional","affiliation":[{"name":"Fondazione G.B. Bietti-IRCCS, Rome - Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Valerio","family":"Carelli","sequence":"additional","affiliation":[{"name":"Dipartimento di Scienze Neurologiche, Universit\u00e0 di Bologna, Bologna - Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alfredo A.","family":"Sadun","sequence":"additional","affiliation":[{"name":"Doheny Eye Institute and Department of Ophthalmology, Keck School of Medicine of the University of the Southern California, Los Angeles, CA - USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2012,4,5]]},"reference":[{"key":"bibr1-ejo.5000154","doi-asserted-by":"publisher","DOI":"10.1016\/j.preteyeres.2003.10.003"},{"key":"bibr2-ejo.5000154","doi-asserted-by":"publisher","DOI":"10.1016\/j.ophtha.2009.07.026"},{"key":"bibr3-ejo.5000154","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.08-2695"},{"key":"bibr4-ejo.5000154","first-page":"51","volume":"104","author":"Sadun A.A.","year":"2006","journal-title":"Trans Am Ophthalmol Soc"},{"key":"bibr5-ejo.5000154","doi-asserted-by":"publisher","DOI":"10.1136\/bjo.2005.074526"},{"key":"bibr6-ejo.5000154","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.05-0455"},{"key":"bibr7-ejo.5000154","doi-asserted-by":"publisher","DOI":"10.1016\/j.ophtha.2004.09.033"},{"key":"bibr8-ejo.5000154","doi-asserted-by":"publisher","DOI":"10.1086\/501236"},{"key":"bibr9-ejo.5000154","doi-asserted-by":"publisher","DOI":"10.1016\/j.ophtha.2007.05.035"},{"key":"bibr10-ejo.5000154","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.04-1174"},{"key":"bibr11-ejo.5000154","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.06-0520"},{"key":"bibr12-ejo.5000154","doi-asserted-by":"publisher","DOI":"10.1016\/j.ophtha.2006.08.046"},{"key":"bibr13-ejo.5000154","first-page":"252","volume":"106","author":"Budenz D.L.","year":"2008","journal-title":"Trans Am Ophthalmol Soc"},{"key":"bibr14-ejo.5000154","doi-asserted-by":"publisher","DOI":"10.1001\/archopht.1983.01040020061011"},{"key":"bibr15-ejo.5000154","doi-asserted-by":"publisher","DOI":"10.1097\/IJG.0b013e3181629a02"},{"key":"bibr16-ejo.5000154","doi-asserted-by":"publisher","DOI":"10.1001\/archopht.1982.01030040575003"},{"key":"bibr17-ejo.5000154","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.08-2142"},{"key":"bibr18-ejo.5000154","doi-asserted-by":"publisher","DOI":"10.1016\/S0161-6420(03)00493-7"},{"key":"bibr19-ejo.5000154","doi-asserted-by":"publisher","DOI":"10.1016\/j.ophtha.2007.02.012"},{"key":"bibr20-ejo.5000154","doi-asserted-by":"publisher","DOI":"10.1016\/S0161-6420(91)32118-3"},{"key":"bibr21-ejo.5000154","doi-asserted-by":"publisher","DOI":"10.1097\/00061198-200010000-00005"},{"key":"bibr22-ejo.5000154","doi-asserted-by":"publisher","DOI":"10.1016\/S0161-6420(93)31497-1"},{"key":"bibr23-ejo.5000154","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9394(14)70488-X"},{"key":"bibr24-ejo.5000154","doi-asserted-by":"publisher","DOI":"10.1016\/S0079-6123(08)01124-2"},{"key":"bibr25-ejo.5000154","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbabio.2009.02.024"}],"container-title":["European Journal of Ophthalmology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.5301\/ejo.5000154","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.5301\/ejo.5000154","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.5301\/ejo.5000154","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T19:36:49Z","timestamp":1777405009000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.5301\/ejo.5000154"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,4,5]]},"references-count":25,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2012,11]]}},"alternative-id":["10.5301\/ejo.5000154"],"URL":"https:\/\/doi.org\/10.5301\/ejo.5000154","relation":{},"ISSN":["1120-6721","1724-6016"],"issn-type":[{"value":"1120-6721","type":"print"},{"value":"1724-6016","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,4,5]]}}}