{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,3]],"date-time":"2026-05-03T05:11:08Z","timestamp":1777785068409,"version":"3.51.4"},"reference-count":25,"publisher":"SAGE Publications","issue":"7","license":[{"start":{"date-parts":[[2013,7,1]],"date-time":"2013-07-01T00:00:00Z","timestamp":1372636800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Int J Artif Organs"],"published-print":{"date-parts":[[2013,7]]},"abstract":"<jats:p>Aseptic loosening of joint prosthetics is one of the most frequent reasons for the failure of total joint replacement surgeries. A major cause of the aseptic loosening is osteolysis caused by a periprosthetic inflammatory response to wear particles released from implanted prosthetics. Tumor necrosis factor (TNF)-\u03b1 is thought to play a dominant role in wear-induced inflammation. It was shown previously by our group, as well as by other researchers, that macrophages produce abundant TNF-\u03b1 when exposed to particulate titanium (Ti), which is widely used as a biomaterial in arthroplastic surgery. In the present study, we have tested the feasibility of using siRNA as a therapeutic intervention against wear-induced TNF-\u03b1 production. Our data show that transfection of U937 macrophage cells with TNF-\u03b1 siRNA inhibits Ti particle-induced expression of TNF-\u03b1 mRNA and protein by &gt;65%. Moreover, U937 cells transfected with TNF-\u03b1 siRNA were significantly more resistant to Ti particle-induced apoptosis (&gt;60%, p&lt;0.05) and caspase-3 activation (&gt;50%, p&lt;0.05) compared with normal U937 cells. Collectively, our data show that siRNA can be an effective way to inhibit Ti particle-induced TNF-\u03b1 expression and the activation of downstream pathways such as apoptosis in macrophages. These data provide a foundation for future studies to investigate the use of siRNA targeting inflammatory cytokines as a therapeutic modality for the treatment of aseptic loosening of prosthetic materials used in arthroplastic surgery.<\/jats:p>","DOI":"10.5301\/ijao.5000218","type":"journal-article","created":{"date-parts":[[2013,5,10]],"date-time":"2013-05-10T05:46:09Z","timestamp":1368164769000},"page":"522-527","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":7,"title":["Suppression of Titanium Particle-Induced TNF-Alpha Expression and Apoptosis in Human U937 Macrophages by siRNA Silencing"],"prefix":"10.1177","volume":"36","author":[{"given":"Kening","family":"Sun","sequence":"first","affiliation":[{"name":"Department of Orthopedics, General Hospital of Ningxia Medical University, Yinchuan - China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yaping","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Orthopedics, General Hospital of Ningxia Medical University, Yinchuan - China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhidong","family":"Lu","sequence":"additional","affiliation":[{"name":"Department of Orthopedics, General Hospital of Ningxia Medical University, Yinchuan - China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Liang","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Orthopedics, General Hospital of Ningxia Medical University, Yinchuan - China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhihui","family":"Gao","sequence":"additional","affiliation":[{"name":"Department of Orthopedics, General Hospital of Ningxia Medical University, Yinchuan - China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qunhua","family":"Jin","sequence":"additional","affiliation":[{"name":"Department of Orthopedics, General Hospital of Ningxia Medical University, Yinchuan - China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2013,10,5]]},"reference":[{"key":"bibr1-ijao.5000218","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2007.06.035"},{"key":"bibr2-ijao.5000218","doi-asserted-by":"publisher","DOI":"10.5435\/00124635-200800001-00003"},{"key":"bibr3-ijao.5000218","doi-asserted-by":"publisher","DOI":"10.1016\/S0883-5403(99)90035-9"},{"key":"bibr4-ijao.5000218","doi-asserted-by":"publisher","DOI":"10.1515\/bmte.1999.44.5.135"},{"issue":"1","key":"bibr5-ijao.5000218","first-page":"25","volume":"71","author":"Kowalczewski J.B.","year":"2006","journal-title":"Chir Narzadow Ruchu Ortop Pol."},{"key":"bibr6-ijao.5000218","doi-asserted-by":"publisher","DOI":"10.1016\/j.advenzreg.2006.01.004"},{"key":"bibr7-ijao.5000218","doi-asserted-by":"publisher","DOI":"10.1016\/0142-9612(96)00072-5"},{"issue":"3","key":"bibr8-ijao.5000218","first-page":"229","volume":"10","author":"Matthews J.B.","year":"2000","journal-title":"Biomed Mater Eng."},{"key":"bibr9-ijao.5000218","doi-asserted-by":"publisher","DOI":"10.1016\/S0142-9612(99)00261-6"},{"key":"bibr10-ijao.5000218","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2012.03.005"},{"issue":"8","key":"bibr11-ijao.5000218","first-page":"1114","volume":"26","author":"Dong L.","year":"2008","journal-title":"Aug"},{"key":"bibr12-ijao.5000218","doi-asserted-by":"publisher","DOI":"10.1007\/s11420-006-9003-6"},{"key":"bibr13-ijao.5000218","doi-asserted-by":"publisher","DOI":"10.1111\/j.1525-1594.2010.01175.x"},{"key":"bibr14-ijao.5000218","doi-asserted-by":"publisher","DOI":"10.1038\/nmat2859"},{"key":"bibr15-ijao.5000218","doi-asserted-by":"publisher","DOI":"10.1016\/j.brainres.2007.04.072"},{"key":"bibr16-ijao.5000218","doi-asserted-by":"publisher","DOI":"10.1038\/mt.2008.220"},{"key":"bibr17-ijao.5000218","doi-asserted-by":"publisher","DOI":"10.1615\/CritRevEukarGeneExpr.v20.i2.10"},{"key":"bibr18-ijao.5000218","doi-asserted-by":"publisher","DOI":"10.1002\/jbm.b.20031"},{"key":"bibr19-ijao.5000218","doi-asserted-by":"publisher","DOI":"10.1016\/S0142-9612(02)00351-4"},{"key":"bibr20-ijao.5000218","doi-asserted-by":"publisher","DOI":"10.1023\/A:1012800813662"},{"key":"bibr21-ijao.5000218","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2003.08.040"},{"key":"bibr22-ijao.5000218","doi-asserted-by":"publisher","DOI":"10.1002\/jbm.a.31315"},{"key":"bibr23-ijao.5000218","doi-asserted-by":"publisher","DOI":"10.5435\/00124635-200800001-00010"},{"key":"bibr24-ijao.5000218","doi-asserted-by":"publisher","DOI":"10.1002\/jor.1100180518"},{"key":"bibr25-ijao.5000218","doi-asserted-by":"publisher","DOI":"10.1016\/S0736-0266(03)00093-7"}],"container-title":["The International Journal of Artificial Organs"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.5301\/ijao.5000218","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.5301\/ijao.5000218","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.5301\/ijao.5000218","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T19:42:47Z","timestamp":1777491767000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.5301\/ijao.5000218"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,7]]},"references-count":25,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2013,7]]}},"alternative-id":["10.5301\/ijao.5000218"],"URL":"https:\/\/doi.org\/10.5301\/ijao.5000218","relation":{},"ISSN":["0391-3988","1724-6040"],"issn-type":[{"value":"0391-3988","type":"print"},{"value":"1724-6040","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,7]]}}}