{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,3]],"date-time":"2026-05-03T04:57:04Z","timestamp":1777784224212,"version":"3.51.4"},"reference-count":27,"publisher":"SAGE Publications","issue":"10","license":[{"start":{"date-parts":[[2015,9,10]],"date-time":"2015-09-10T00:00:00Z","timestamp":1441843200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Int J Artif Organs"],"published-print":{"date-parts":[[2015,10]]},"abstract":"<jats:sec>\n                    <jats:title>Purpose<\/jats:title>\n                    <jats:p>Peritoneal dialysis fluids (PDFs) may induce inadequate heat-shock protein (HSP) expression and injury-related inflammation in exposed mesothelial cells. The aim of this study was to relate cellular injury to these cellular responses in mesothelial cells following repeated exposure to 3 commercial PDFs with different biocompatibility profiles.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Primary cultures of human peritoneal mesothelial cells (HPMC) were exposed to a 1:2 mixture of cell culture medium and CAPD2 (single-chamber bag PDF; Fresenius, Bad Homburg, Germany), Physioneal (dual-chamber bag PDF; Baxter, Deerfield, IL, USA) or Balance (dual-chamber bag PDF, Fresenius) for up to 10 days exposure time (4 dwells). Supernatant was analyzed for LDH, IL-6, and IL-8, cells for HSP-72 expression, and protein content.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>PDF exposure resulted in a biphasic pattern of cell damage switching from an earlier phase with increased injury by single-chamber PDF to a delayed phase with increased susceptibility to dual-chamber PDF. Sterile inflammation was related to LDH release over time and could be reproduced by exposure to necrotic cellular material. PDF exposure resulted in low HSP-72 expression in all tested PDFs.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Exposure to single-chamber as well as to dual-chamber bag PDFs induce increased vulnerability of mesothelial cells to repeated exposure of the same solution. These effects were delayed with dual-chamber PDFs. Injury-induced inflammation and impaired HSP expression upon PDF exposure might initiate a vicious cycle with progredient mesothelial cell damage upon repeated PDF exposure. Certainly, interventional studies and translation of these results into the in vivo system is needed.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.5301\/ijao.5000438","type":"journal-article","created":{"date-parts":[[2015,10,20]],"date-time":"2015-10-20T13:50:39Z","timestamp":1445349039000},"page":"530-536","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":3,"title":["Injury-Induced Inflammation and Inadequate HSP Expression in Mesothelial Cells upon Repeat Exposure to Dual-Chamber Bag Peritoneal Dialysis Fluids"],"prefix":"10.1177","volume":"38","author":[{"given":"Thorsten O.","family":"Bender","sequence":"first","affiliation":[{"name":"Department of Nephrology and Medical Intensive Care, Charit\u00e9 Universit\u00e4tsmedizin Berlin, Campus Virchow-Klinikum, Berlin - Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Klaus","family":"Kratochwill","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Medical University, Vienna - Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rebecca","family":"Herzog","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Medical University, Vienna - Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andrea","family":"Ulbrich","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Medical University, Vienna - Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael","family":"B\u00f6hm","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Medical University, Vienna - Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Achim","family":"J\u00f6rres","sequence":"additional","affiliation":[{"name":"Department of Nephrology and Medical Intensive Care, Charit\u00e9 Universit\u00e4tsmedizin Berlin, Campus Virchow-Klinikum, Berlin - Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christoph","family":"Aufricht","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Medical University, Vienna - Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2015,9,10]]},"reference":[{"key":"bibr1-ijao.5000438","doi-asserted-by":"publisher","DOI":"10.1177\/089686080902902S12"},{"issue":"5","key":"bibr2-ijao.5000438","doi-asserted-by":"crossref","first-page":"1046","DOI":"10.1681\/ASN.V1251046","volume":"12","author":"Davies S.J.","year":"2001","journal-title":"J Am Soc Nephrol."},{"key":"bibr3-ijao.5000438","doi-asserted-by":"publisher","DOI":"10.1177\/089686080102100115"},{"key":"bibr4-ijao.5000438","doi-asserted-by":"publisher","DOI":"10.1046\/j.1523-1755.2001.00004.x"},{"key":"bibr5-ijao.5000438","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.00503.2005"},{"key":"bibr6-ijao.5000438","doi-asserted-by":"publisher","DOI":"10.1111\/j.1523-1755.2004.66040.x"},{"key":"bibr7-ijao.5000438","doi-asserted-by":"publisher","DOI":"10.5301\/IJAO.2011.8379"},{"key":"bibr8-ijao.5000438","doi-asserted-by":"publisher","DOI":"10.1093\/ndt\/gfq484"},{"key":"bibr9-ijao.5000438","doi-asserted-by":"publisher","DOI":"10.1177\/089686089301300309"},{"key":"bibr10-ijao.5000438","doi-asserted-by":"publisher","DOI":"10.1177\/089686080002002S09"},{"key":"bibr11-ijao.5000438","doi-asserted-by":"publisher","DOI":"10.3747\/pdi.2012.00331"},{"key":"bibr12-ijao.5000438","doi-asserted-by":"publisher","DOI":"10.1038\/ki.1990.150"},{"key":"bibr13-ijao.5000438","doi-asserted-by":"publisher","DOI":"10.1152\/japplphysiol.01086.2005"},{"issue":"4","key":"bibr14-ijao.5000438","doi-asserted-by":"crossref","first-page":"381","DOI":"10.1177\/089686080302300412","volume":"23","author":"Witowski J.","year":"2003","journal-title":"Perit Dial Int."},{"key":"bibr15-ijao.5000438","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajpath.2010.12.034"},{"key":"bibr16-ijao.5000438","doi-asserted-by":"publisher","DOI":"10.1177\/039139880703000304"},{"key":"bibr17-ijao.5000438","doi-asserted-by":"publisher","DOI":"10.1007\/s10157-008-0032-y"},{"key":"bibr18-ijao.5000438","doi-asserted-by":"publisher","DOI":"10.1159\/000342523"},{"key":"bibr19-ijao.5000438","doi-asserted-by":"publisher","DOI":"10.1093\/ndt\/gfs314"},{"key":"bibr20-ijao.5000438","doi-asserted-by":"publisher","DOI":"10.1177\/089686080802800214"},{"key":"bibr21-ijao.5000438","doi-asserted-by":"publisher","DOI":"10.1159\/000084110"},{"key":"bibr22-ijao.5000438","doi-asserted-by":"publisher","DOI":"10.1111\/j.1523-1755.2004.00747.x"},{"key":"bibr23-ijao.5000438","doi-asserted-by":"publisher","DOI":"10.1093\/ndt\/gfq003"},{"key":"bibr24-ijao.5000438","doi-asserted-by":"publisher","DOI":"10.1016\/j.cca.2013.12.003"},{"issue":"11","key":"bibr25-ijao.5000438","doi-asserted-by":"crossref","first-page":"2434","DOI":"10.1681\/ASN.V12112434","volume":"12","author":"Witowski J.","year":"2001","journal-title":"J Am Soc Nephrol."},{"key":"bibr26-ijao.5000438","doi-asserted-by":"publisher","DOI":"10.1021\/pr800916s"},{"key":"bibr27-ijao.5000438","doi-asserted-by":"publisher","DOI":"10.1126\/science.1071059"}],"container-title":["The International Journal of Artificial Organs"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.5301\/ijao.5000438","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.5301\/ijao.5000438","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.5301\/ijao.5000438","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T19:39:59Z","timestamp":1777491599000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.5301\/ijao.5000438"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,9,10]]},"references-count":27,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2015,10]]}},"alternative-id":["10.5301\/ijao.5000438"],"URL":"https:\/\/doi.org\/10.5301\/ijao.5000438","relation":{},"ISSN":["0391-3988","1724-6040"],"issn-type":[{"value":"0391-3988","type":"print"},{"value":"1724-6040","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,9,10]]}}}