{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,3]],"date-time":"2026-05-03T05:02:32Z","timestamp":1777784552620,"version":"3.51.4"},"reference-count":39,"publisher":"SAGE Publications","issue":"9","license":[{"start":{"date-parts":[[2017,6,14]],"date-time":"2017-06-14T00:00:00Z","timestamp":1497398400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Int J Artif Organs"],"published-print":{"date-parts":[[2017,9]]},"abstract":"<jats:sec>\n                    <jats:title>Introduction<\/jats:title>\n                    <jats:p>Healing of airway anastomoses after preoperative irradiation can be a significant clinical problem. The augmentation of bronchial anastomoses with a fibroblast-seeded human acellular dermis (hAD) was shown to be beneficial, although the underlying mechanism remained unclear. Therefore, in this study we investigated the fate of the fibroblasts transplanted to the scaffold covering the anastomosis.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Material and methods<\/jats:title>\n                    <jats:p>32 Fisher rats underwent surgical anastomosis of the left main bronchus. In a 2 \u00d7 2 factorial design, they were randomized to receive preoperative irradiation of 20 Gy and augmentation of the anastomosis with a fibroblast-seeded transplant. Fibroblasts from subcutaneous fat of Fischer-344 rat were transduced retrovirally with tdTomato for cell tracking. After 7 and 14 days, animals were sacrificed and cell concentration of transplanted and nontransplanted fibroblasts in the hAD as well as in the bronchial tissue was measured using RT-PCR.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Migration of transplanted fibroblasts from dermis to bronchus were demonstrated in both groups, irradiated and nonirradiated. In the irradiated groups, there was a cell count of 7 \u00d7 10\n                      <jats:sup>4<\/jats:sup>\n                      \u00b1 1 \u00d7 10\n                      <jats:sup>4<\/jats:sup>\n                      tomato\n                      <jats:sup>+<\/jats:sup>\n                      -fibroblasts in the bronchial tissue at day 7, rising to 1 \u00d7 10\n                      <jats:sup>5<\/jats:sup>\n                      \u00b1 1 \u00d7 10\n                      <jats:sup>4<\/jats:sup>\n                      on day 14 (p&lt;0.0001). Tomato\n                      <jats:sup>+<\/jats:sup>\n                      -cell concentration in hAD increased from 6 \u00d7 10\n                      <jats:sup>3<\/jats:sup>\n                      \u00b1 1 \u00d7 10\n                      <jats:sup>3<\/jats:sup>\n                      at day 7 to 6 \u00d7 10\n                      <jats:sup>4<\/jats:sup>\n                      \u00b1 1 \u00d7 10\n                      <jats:sup>4<\/jats:sup>\n                      at day 14 (p&lt;0.0001). In the nonirradiated groups, tomato\n                      <jats:sup>+<\/jats:sup>\n                      -cell concentration in bronchus was 4 \u00d7 10\n                      <jats:sup>3<\/jats:sup>\n                      \u00b1 1 \u00d7 10\n                      <jats:sup>3<\/jats:sup>\n                      on day 7 and 4 \u00d7 10\n                      <jats:sup>3<\/jats:sup>\n                      \u00b1 1 \u00d7 10\n                      <jats:sup>3<\/jats:sup>\n                      at day 14. In the hAD tomato\n                      <jats:sup>+<\/jats:sup>\n                      cell concentration rising from 1 \u00d7 10\n                      <jats:sup>4<\/jats:sup>\n                      \u00b1 1 \u00d7 10\n                      <jats:sup>3<\/jats:sup>\n                      at day 7 to 2 \u00d7 10\n                      <jats:sup>4<\/jats:sup>\n                      \u00b1 3 \u00d7 10\n                      <jats:sup>3<\/jats:sup>\n                      cells at day 14 (p = 0.0028).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Transplanted fibroblasts in the irradiated groups proliferate and migrate into the irradiated host bronchial tissue, but not in the nonirradiated groups.<\/jats:p>\n                  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