{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,19]],"date-time":"2026-09-19T07:34:25Z","timestamp":1789803265012,"version":"4.0.1"},"reference-count":34,"publisher":"SAGE Publications","issue":"4","license":[{"start":{"date-parts":[[2014,9,12]],"date-time":"2014-09-12T00:00:00Z","timestamp":1410480000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Int J Biol Markers"],"published-print":{"date-parts":[[2014,10]]},"abstract":"<jats:sec>\n                    <jats:title\/>\n                    <jats:p>We assessed the suitability of HS3ST2 and CCNA1 genes as biomarkers for the early detection of cervical cancer in Uygur women in Xinjiang, China. Methylation-specific PCR (MSP) and HPV (HPV16 and HPV18)\u2013specific PCR were performed on 110 cervical samples: 40 normal cervices, 10 cervical intraepithelial neoplasia 1 (CIN1), 10 CIN2, 10 CIN3 and 40 cervical cancer tissues. The expression of the 2 genes was measured by reverse transcription PCR (RT-PCR) in 10 methylation-positive and 10 methylation-negative cervical tissues. We found that both HS3ST2 and CCNA1 genes were methylated in 38 of the 40 cervical cancer tissues, 9 of the 10 CIN3, and 6 of the 10 CIN2. In contrast, methylation of these 2 genes was found in only 1 of the 40 normal tissues and none of 10 CIN1. Furthermore, hypermethylated HS3ST2 and CCNA1 genes were correlated with infection with HPV16 and HPV18 in high-grade squamous intraepithelial lesions (HSILs) and cervical cancer (both p&lt;0.05). The expression of HS3ST2 and CCNA1 genes was lower in the methylation-positive cervical tissues than in the methylation-negative cervical tissues. Our results indicate that HS3ST2 and CCNA1 genes may play important roles in HPV-induced cervical cancer and that patients with specific hypermethylated genes may have a greater risk of progressing to invasive cervical cancer.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.5301\/jbm.5000107","type":"journal-article","created":{"date-parts":[[2014,11,3]],"date-time":"2014-11-03T04:25:13Z","timestamp":1414988713000},"page":"354-362","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":8,"title":["Methylation in the Promoters of\n                    <i>HS3ST2<\/i>\n                    and\n                    <i>CCNA1<\/i>\n                    Genes is Associated with Cervical Cancer in Uygur Women in Xinjiang"],"prefix":"10.1177","volume":"29","author":[{"given":"Qiangqiang","family":"Zuo","sequence":"first","affiliation":[{"name":"Department of Biochemistry, School of Medicine, Shihezi University, Xinjiang Endemic and Ethnic Disease and Education Ministry Key Laboratory, Shihezi, Xinjiang - PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weinan","family":"Zheng","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, School of Medicine, Shihezi University, Xinjiang Endemic and Ethnic Disease and Education Ministry Key Laboratory, Shihezi, Xinjiang - PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinli","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, School of Medicine, Shihezi University, Xinjiang Endemic and Ethnic Disease and Education Ministry Key Laboratory, Shihezi, Xinjiang - PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zemin","family":"Pan","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, School of Medicine, Shihezi University, Xinjiang Endemic and Ethnic Disease and Education Ministry Key Laboratory, Shihezi, Xinjiang - PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yinyin","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, School of Medicine, Shihezi University, Xinjiang Endemic and Ethnic Disease and Education Ministry Key Laboratory, Shihezi, Xinjiang - PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haichen","family":"Long","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, School of Medicine, Shihezi University, Xinjiang Endemic and Ethnic Disease and Education Ministry Key Laboratory, Shihezi, Xinjiang - PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peiwen","family":"Fan","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, School of Medicine, Shihezi University, Xinjiang Endemic and Ethnic Disease and Education Ministry Key Laboratory, Shihezi, Xinjiang - PR 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