{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,3,3]],"date-time":"2025-03-03T05:37:28Z","timestamp":1740980248954,"version":"3.38.0"},"reference-count":28,"publisher":"SAGE Publications","issue":"4","license":[{"start":{"date-parts":[[2013,2,4]],"date-time":"2013-02-04T00:00:00Z","timestamp":1359936000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["European Journal of Ophthalmology"],"published-print":{"date-parts":[[2013,7]]},"abstract":"<jats:sec><jats:title>Purpose<\/jats:title><jats:p> To evaluate the use of a femtosecond laser combined with a microkeratome in the preparation of posterior corneal disks for Descemet stripping automated endothelial keratoplasty (DSAEK). <\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p> This experimental study involved ultrathin DSAEK tissue preparation of 22 donor corneas unsuitable for transplantation. The first cut was performed with an Intralase<jats:sup>\u00ae<\/jats:sup> FS60 laser and the second cut with a Moria CBm 300-\u03bcm microkeratome. The thickness of the first cut was modified for each cornea to obtain a final graft thickness of less than 110 \u03bcm. Precut and postcut central pachymetry were performed with an ultrasonic pachymeter. Central endothelial cell density (ECD) was calculated before and 24 hours after tissue preparation. <\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p> Final graft thickness was 105.0 \u00b1 26.1 (SD) \u03bcm (range 65\u2013117). The mean microkeratome head cut thickness was 324.5 \u00b1 10.9 \u03bcm (range 310\u2013345). Precut and postcut ECDs averaged 2250 \u00b1 222 and 2093 \u00b1 286 cells\/mm<jats:sup>2<\/jats:sup>, respectively, representing 6.9% of cell loss. No corneas were perforated. <\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p> Femtosecond FS60 lasers and Moria CBm 300-\u03bcm microkeratomes can be used sequentially to prepare consistently thin DSAEK grafts with no irregular cuts or cornea perforations. <\/jats:p><\/jats:sec>","DOI":"10.5301\/ejo.5000273","type":"journal-article","created":{"date-parts":[[2013,6,14]],"date-time":"2013-06-14T08:56:52Z","timestamp":1371200212000},"page":"584-589","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":4,"title":["Combined Use of a Femtosecond Laser and a Microkeratome in Obtaining Thin Grafts for Descemet Stripping Automated Endothelial Keratoplasty: An Eye Bank Study"],"prefix":"10.1177","volume":"23","author":[{"given":"Joaquim N.","family":"Murta","sequence":"first","affiliation":[{"name":"Ophthalmology Unit, Centro Hospitalar e Universit\u00e1rio de Coimbra, Coimbra - Portugal"},{"name":"Centro Cir\u00fargico de Coimbra, Coimbra - Portugal"},{"name":"Faculty of Medicine, University of Coimbra, Coimbra - Portugal"}]},{"given":"Andreia M.","family":"Rosa","sequence":"additional","affiliation":[{"name":"Ophthalmology Unit, Centro Hospitalar e Universit\u00e1rio de Coimbra, Coimbra - Portugal"},{"name":"Centro Cir\u00fargico de Coimbra, Coimbra - Portugal"},{"name":"Faculty of Medicine, University of Coimbra, Coimbra - Portugal"}]},{"given":"Maria Joao C.","family":"Quadrado","sequence":"additional","affiliation":[{"name":"Ophthalmology Unit, Centro Hospitalar e Universit\u00e1rio de Coimbra, Coimbra - Portugal"},{"name":"Centro Cir\u00fargico de Coimbra, Coimbra - Portugal"},{"name":"Faculty of Medicine, University of Coimbra, Coimbra - Portugal"}]},{"given":"Ana D.","family":"Russo","sequence":"additional","affiliation":[{"name":"Ophthalmology Unit, Centro Hospitalar e Universit\u00e1rio de Coimbra, Coimbra - Portugal"}]},{"given":"Sergio S.","family":"Brito","sequence":"additional","affiliation":[{"name":"Ophthalmology Unit, Centro Hospitalar e Universit\u00e1rio de Coimbra, Coimbra - Portugal"}]},{"given":"Maria F\u00e1tima L.","family":"Silva","sequence":"additional","affiliation":[{"name":"Visual Neuroscience Laboratory, Institute of Biomedical Research on Light and Image, Faculty of Medicine, University of Coimbra, Coimbra - Portugal"}]}],"member":"179","published-online":{"date-parts":[[2013,2,4]]},"reference":[{"key":"bibr1-ejo.5000273","doi-asserted-by":"publisher","DOI":"10.1016\/j.ophtha.2009.06.021"},{"key":"bibr2-ejo.5000273","doi-asserted-by":"publisher","DOI":"10.1016\/j.ophtha.2006.05.034"},{"key":"bibr3-ejo.5000273","doi-asserted-by":"publisher","DOI":"10.1097\/01.ico.0000214224.90743.01"},{"key":"bibr4-ejo.5000273","doi-asserted-by":"publisher","DOI":"10.1097\/01.ico.0000243962.60392.4f"},{"key":"bibr5-ejo.5000273","doi-asserted-by":"publisher","DOI":"10.1097\/ICU.0b013e32832b8d18"},{"key":"bibr6-ejo.5000273","doi-asserted-by":"publisher","DOI":"10.1097\/ICO.0b013e31802cd8c2"},{"key":"bibr7-ejo.5000273","doi-asserted-by":"publisher","DOI":"10.1097\/ICO.0b013e3181611c50"},{"key":"bibr8-ejo.5000273","doi-asserted-by":"publisher","DOI":"10.1097\/ICO.0b013e3181cda01a"},{"key":"bibr9-ejo.5000273","doi-asserted-by":"publisher","DOI":"10.1136\/bjo.2008.147389"},{"key":"bibr10-ejo.5000273","doi-asserted-by":"publisher","DOI":"10.1097\/ICO.0b013e3181f236c6"},{"key":"bibr11-ejo.5000273","doi-asserted-by":"publisher","DOI":"10.1097\/ICO.0b013e31821821c7"},{"key":"bibr12-ejo.5000273","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajo.2011.05.003"},{"key":"bibr13-ejo.5000273","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajo.2010.05.011"},{"key":"bibr14-ejo.5000273","doi-asserted-by":"publisher","DOI":"10.1136\/bjophthalmol-2011-300462"},{"key":"bibr15-ejo.5000273","doi-asserted-by":"publisher","DOI":"10.1016\/j.oftal.2010.12.012"},{"key":"bibr16-ejo.5000273","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcrs.2005.12.078"},{"key":"bibr17-ejo.5000273","doi-asserted-by":"publisher","DOI":"10.1016\/S0161-6420(02)01939-5"},{"key":"bibr18-ejo.5000273","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.11-7753"},{"key":"bibr19-ejo.5000273","first-page":"425","volume":"6","author":"Hsu M.","year":"2012","journal-title":"Clin Ophthalmol"},{"key":"bibr20-ejo.5000273","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajo.2011.01.051"},{"key":"bibr21-ejo.5000273","doi-asserted-by":"publisher","DOI":"10.2174\/1874364101206010019"},{"key":"bibr22-ejo.5000273","doi-asserted-by":"publisher","DOI":"10.1097\/ICO.0b013e3181f22cc3"},{"key":"bibr23-ejo.5000273","doi-asserted-by":"publisher","DOI":"10.1097\/ICO.0b013e31815771f5"},{"key":"bibr24-ejo.5000273","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcrs.2008.12.038"},{"key":"bibr25-ejo.5000273","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcrs.2008.08.021"},{"key":"bibr26-ejo.5000273","doi-asserted-by":"publisher","DOI":"10.1097\/ICO.0b013e31819c4fcf"},{"key":"bibr27-ejo.5000273","doi-asserted-by":"publisher","DOI":"10.1016\/j.ophtha.2007.11.032"},{"key":"bibr28-ejo.5000273","doi-asserted-by":"publisher","DOI":"10.1016\/j.ophtha.2007.04.020"}],"container-title":["European Journal of Ophthalmology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.5301\/ejo.5000273","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.5301\/ejo.5000273","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.5301\/ejo.5000273","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,3,2]],"date-time":"2025-03-02T11:22:45Z","timestamp":1740914565000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.5301\/ejo.5000273"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,2,4]]},"references-count":28,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2013,7]]}},"alternative-id":["10.5301\/ejo.5000273"],"URL":"https:\/\/doi.org\/10.5301\/ejo.5000273","relation":{},"ISSN":["1120-6721","1724-6016"],"issn-type":[{"type":"print","value":"1120-6721"},{"type":"electronic","value":"1724-6016"}],"subject":[],"published":{"date-parts":[[2013,2,4]]}}}