{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,30]],"date-time":"2026-01-30T05:26:05Z","timestamp":1769750765685,"version":"3.49.0"},"reference-count":39,"publisher":"SAGE Publications","issue":"3","license":[{"start":{"date-parts":[[2017,1,1]],"date-time":"2017-01-01T00:00:00Z","timestamp":1483228800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Journal of Applied Biomaterials &amp; Functional Materials"],"published-print":{"date-parts":[[2017,1]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p> In recent years, the tissue engineering (TE) field has significantly benefited from advanced techniques such as additive manufacturing (AM), for the design of customized 3D scaffolds with the aim of guided tissue repair. Among the wide range of materials available to biomanufacture 3D scaffolds, poly(\u03b5-caprolactone) (PCL) clearly arises as the synthetic polymer with the greatest potential, due to its unique properties \u2013 namely, biocompatibility, biodegradability, thermal and chemical stability and processability. This study aimed for the first time to investigate the effect of pore geometry on the in vitro enzymatic chain cleavage mechanism of PCL scaffolds manufactured by the AM extrusion process. <\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p> Methods: Morphological properties of 3D printed PCL scaffolds before and after degradation were evaluated using Scanning Electron Microscopy (SEM) and micro-computed tomography (\u03bc-CT). Differential Scanning Calorimetry (DSC) was employed to determine possible variations in the crystallinity of the scaffolds during the degradation period. The molecular weight was assessed using Size Exclusion Chromatography (SEC) while the mechanical properties were investigated under static compression conditions. <\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p> Morphological results suggested a uniform reduction of filament diameter, while increasing the scaffolds\u2019 porosity. DSC analysis revealed and increment in the crystallinity degree while the molecular weight, evaluated through SEC, remained almost constant during the incubation period (25 days). Mechanical analysis highlighted a decrease in the compressive modulus and maximum stress over time, probably related to the significant weight loss of the scaffolds. <\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p> All of these results suggest that PCL scaffolds undergo enzymatic degradation through a surface erosion mechanism, which leads to significant variations in mechanical, physical and chemical properties, but which has little influence on pore geometry. <\/jats:p><\/jats:sec>","DOI":"10.5301\/jabfm.5000363","type":"journal-article","created":{"date-parts":[[2017,6,16]],"date-time":"2017-06-16T12:39:04Z","timestamp":1497616744000},"page":"185-195","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":17,"title":["Effect of in Vitro Enzymatic Degradation on 3D Printed Poly(\u03b5-Caprolactone) Scaffolds: Morphological, Chemical and Mechanical Properties"],"prefix":"10.1177","volume":"15","author":[{"given":"Joana","family":"Ferreira","sequence":"first","affiliation":[{"name":"Centre for Rapid and Sustainable Product Development, Polytechnic Institute of Leiria, Leiria - Portugal"}]},{"given":"Antonio","family":"Gloria","sequence":"additional","affiliation":[{"name":"Institute of Polymers, Composites and Biomaterials, National Research Council of Italy, Naples - Italy"}]},{"given":"Stefania","family":"Cometa","sequence":"additional","affiliation":[{"name":"Jaber Innovation s.r.l., Rome - Italy"}]},{"given":"Jorge F.J.","family":"Coelho","sequence":"additional","affiliation":[{"name":"CEMUC-Chemical Engineering Department, University of Coimbra, Coimbra - Portugal"}]},{"given":"Marco","family":"Domingos","sequence":"additional","affiliation":[{"name":"School of Mechanical, Aerospace and Civil Engineering, University of Manchester, Manchester - UK"}]}],"member":"179","published-online":{"date-parts":[[2017,6,15]]},"reference":[{"key":"bibr1-jabfm.5000363","doi-asserted-by":"publisher","DOI":"10.1126\/science.8493529"},{"key":"bibr2-jabfm.5000363","doi-asserted-by":"publisher","DOI":"10.1002\/1616-5195(20020201)2:2<67::AID-MABI67>3.0.CO;2-F"},{"key":"bibr3-jabfm.5000363","unstructured":"BrownJ KumbarS BanikB Bio-instructive scaffolds for musculoskeletal tissue engineering and regenerative medicine. 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