{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,3]],"date-time":"2026-06-03T09:15:38Z","timestamp":1780478138930,"version":"3.54.1"},"reference-count":31,"publisher":"American Animal Hospital Association","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of the American Animal Hospital Association"],"published-print":{"date-parts":[[2004,9]]},"abstract":"<jats:p>Serological tests were performed on 380 cats with necropsy-confirmed heartworm status to compare the performance of currently available commercial laboratory and point-of-care heart-worm serological tests in a heartworm-endemic area. Overall, antigen tests detected 79.3% to 86.2% of heartworm infections and were highly specific. Most cats with false-negative antigen tests had a single male worm. Antibody tests detected 62.1% to 72.4% of heartworm infections and had a wider range of false-positive results (1.4% to 19.1%) than antigen tests (0.3% to 2.0%). Serological tests for feline heartworm infection varied in diagnostic performance. Combining results from antigen and antibody tests achieved greater sensitivity than using either test alone.<\/jats:p>","DOI":"10.5326\/0400376","type":"journal-article","created":{"date-parts":[[2014,9,15]],"date-time":"2014-09-15T12:15:46Z","timestamp":1410783346000},"page":"376-384","source":"Crossref","is-referenced-by-count":49,"title":["Comparison of Serological Tests for the Detection of Natural Heartworm Infection in Cats"],"prefix":"10.5326","volume":"40","author":[{"given":"Paul","family":"Berdoulay","sequence":"first","affiliation":[{"name":"Department of Small Animal, Clinical Sciences, (Berdoulay, Levy, Pegelow, Hooks, Tavares, Salute), College of Veterinary Medicine, University of Florida, Gainesville, Florida 32610"},{"name":"Jacksonville Veterinary Internal, Medicine and Referral Center (Snyder), Orange Park, Florida 32073; and the"},{"name":"Department of Educational Leadership, (Gibson), Valdosta State University, Valdosta, Florida 31698"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julie K.","family":"Levy","sequence":"additional","affiliation":[{"name":"Department of Small Animal, Clinical Sciences, (Berdoulay, Levy, Pegelow, Hooks, Tavares, Salute), College of Veterinary Medicine, University of Florida, Gainesville, Florida 32610"},{"name":"Jacksonville Veterinary Internal, Medicine and Referral Center (Snyder), Orange Park, Florida 32073; and the"},{"name":"Department of Educational Leadership, (Gibson), Valdosta State University, Valdosta, Florida 31698"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patti S.","family":"Snyder","sequence":"additional","affiliation":[{"name":"Department of Small Animal, Clinical Sciences, (Berdoulay, Levy, Pegelow, Hooks, Tavares, Salute), College of Veterinary Medicine, University of Florida, Gainesville, Florida 32610"},{"name":"Jacksonville Veterinary Internal, Medicine and Referral Center (Snyder), Orange Park, Florida 32073; and the"},{"name":"Department of Educational Leadership, (Gibson), Valdosta State University, Valdosta, Florida 31698"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael J.","family":"Pegelow","sequence":"additional","affiliation":[{"name":"Department of Small Animal, Clinical Sciences, (Berdoulay, Levy, Pegelow, Hooks, Tavares, Salute), College of Veterinary Medicine, University of Florida, Gainesville, Florida 32610"},{"name":"Jacksonville Veterinary Internal, Medicine and Referral Center (Snyder), Orange Park, Florida 32073; and the"},{"name":"Department of Educational Leadership, (Gibson), Valdosta State University, Valdosta, Florida 31698"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jennifer L.","family":"Hooks","sequence":"additional","affiliation":[{"name":"Department of Small Animal, Clinical Sciences, (Berdoulay, Levy, Pegelow, Hooks, Tavares, Salute), College of Veterinary Medicine, University of Florida, Gainesville, Florida 32610"},{"name":"Jacksonville Veterinary Internal, Medicine and Referral Center (Snyder), Orange Park, Florida 32073; and the"},{"name":"Department of Educational Leadership, (Gibson), Valdosta State University, Valdosta, Florida 31698"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Larissa M.","family":"Tavares","sequence":"additional","affiliation":[{"name":"Department of Small Animal, Clinical Sciences, (Berdoulay, Levy, Pegelow, Hooks, Tavares, Salute), College of Veterinary Medicine, University of Florida, Gainesville, Florida 32610"},{"name":"Jacksonville Veterinary Internal, Medicine and Referral Center (Snyder), Orange Park, Florida 32073; and the"},{"name":"Department of Educational Leadership, (Gibson), Valdosta State University, Valdosta, Florida 31698"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicole M.","family":"Gibson","sequence":"additional","affiliation":[{"name":"Department of Small Animal, Clinical Sciences, (Berdoulay, Levy, Pegelow, Hooks, Tavares, Salute), College of Veterinary Medicine, University of Florida, Gainesville, Florida 32610"},{"name":"Jacksonville Veterinary Internal, Medicine and Referral Center (Snyder), Orange Park, Florida 32073; and the"},{"name":"Department of Educational Leadership, (Gibson), Valdosta State University, Valdosta, Florida 31698"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marc E.","family":"Salute","sequence":"additional","affiliation":[{"name":"Department of Small Animal, Clinical Sciences, (Berdoulay, Levy, Pegelow, Hooks, Tavares, Salute), College of Veterinary Medicine, University of Florida, Gainesville, Florida 32610"},{"name":"Jacksonville Veterinary Internal, Medicine and Referral Center (Snyder), Orange Park, Florida 32073; and the"},{"name":"Department of Educational Leadership, (Gibson), Valdosta State University, Valdosta, Florida 31698"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"3280","reference":[{"key":"R1","year":"1995","unstructured":"Ryan WG, Newcomb KM. Prevalence of feline heartworm disease\u2013a global review. In: Proceedings, Heartworm Symposium 1995. Batavia, Ill: American Heartworm Society,\n          1995:79\u201384."},{"key":"R2","series-title":"J Am Vet Med Assoc","first-page":"913","volume":"192","year":"1988","unstructured":"Willard MD, Roberts RE, Allison N, et al. Diagnosis of Aelurostrongylus abstrusus and Dirofilaria immitus infections in cats from a humane shelter.\n          J Am Vet Med Assoc\n          1988;192:913\u2013916."},{"key":"R3","series-title":"J Am Vet Med Assoc","first-page":"211","volume":"217","year":"2000","unstructured":"Hermesmeyer M, Limberg-Child RK, Murphy AJ, et al. Prevalence of Dirofilaria immitis infection among shelter cats.\n          J Am Vet Med Assoc\n          2000;217:211\u2013212."},{"key":"R4","series-title":"Compend Contin Educ Pract Vet","first-page":"585","volume":"10","year":"1988","unstructured":"Elkins AD, Kadel W. Feline heartworm disease and its incidence in western Kentucky.\n          Compend Contin Educ Pract Vet\n          1988;10:585\u2013589."},{"key":"R5","series-title":"Vet Clin North Am Small Anim Pract","first-page":"125","volume":"23","year":"1993","unstructured":"Holmes RA. Feline dirofilariasis.\n          Vet Clin North Am Small Anim Pract\n          1993;23:125\u2013138."},{"key":"R6","series-title":"Am Heartworm Soc Bulletin","first-page":"2","volume":"29","year":"2002","unstructured":"Knight DH, Downing R, Nelson CT, et al. 2002 guidelines for the diagnosis, prevention, and management of heartworm (Dirofilaria immitis) infection in cats.\n          Am Heartworm Soc Bulletin\n          2002;29:2\u201311."},{"key":"R7","series-title":"Vet Clin North Am Small Anim Pract","first-page":"1547","volume":"28","year":"1998","unstructured":"Dillon R. Clinical significance of feline heartworm disease.\n          Vet Clin North Am Small Anim Pract\n          1998;28:1547\u20131565."},{"key":"R8","year":"1998","unstructured":"Dillon R, Brawner WR, Robertson-Plouch CK, et al. Feline heart-worm disease: correlations, clinical signs, serology, and other diagnostics \u2013 results of a multicenter study. In: Proceedings, Heartworm Symposium 1998. Batavia, Ill: American Heartworm Society,\n          1998:153\u2013157."},{"key":"R9","year":"1992","unstructured":"Holmes RA, Clark JN, Casey HW, et al. Histopathologic and radiographic studies of the temporal development of heartworm pulmonary vascular disease in experimentally infected cats. In: Proceedings, Heartworm Symposium 1992. Washington DC: American Heartworm Society,\n          1992:81\u201389."},{"key":"R10","year":"1998","unstructured":"Brawner WR, Dillon AR, Robertson-Plouch CK, et al. Radiographic diagnosis of feline heartworm disease and correlation to other clinical criteria: results of a multicenter clinical case study. In: Proceedings, Heartworm Symposium 1998. Batavia, Ill: American Heartworm Society,\n          1998:91\u201395."},{"key":"R11","year":"1998","unstructured":"Atkins CE, DeFrancesco TC, Coats JR, et al. Feline heartworm disease: the North Carolina experience. In: Proceedings, Heartworm Symposium 1998. Batavia, Ill: American Heartworm Society,\n          1998:123\u2013125."},{"key":"R12","year":"1998","unstructured":"DeFrancesco TC, Atkins CE. The utility of echocardiography in the diagnosis of feline heartworm disease: a review of published reports. In: Proceedings, Heartworm Symposium 1998. Batavia, Ill: American Heartworm Society,\n          1998:103\u2013105."},{"key":"R13","series-title":"J Vet Intern Med","first-page":"141","volume":"11","year":"1997","unstructured":"DeFrancesco TC, Atkins CE, Meurs K, et al. Diagnostic utility of echocardiography in feline heartworm disease.\n          J Vet Intern Med\n          1997;11:141."},{"key":"R14","series-title":"J Am Vet Med Assoc","first-page":"66","volume":"28","year":"2001","unstructured":"DeFrancesco TC, Atkins CE, Miller MW, et al. Use of echocardiography for the diagnosis of heartworm disease in cats: 43 cases (1985\u20131997).\n          J Am Vet Med Assoc\n          2001;28:66\u201369."},{"key":"R15","series-title":"J Am Vet Med Assoc","first-page":"517","volume":"212","year":"1998","unstructured":"Atkins CA, DeFrancesco TC, Miller M, et al. Prevalence of heart-worm infection in cats with cardiorespiratory abnormalities.\n          J Am Vet Med Assoc\n          1998;212:517\u2013520."},{"key":"R16","year":"1992","unstructured":"McCall JW, Dzimianski MT, McTeir TL, et al. Biology of experimental heartworm infections in cats. In: Proceedings, Heartworm Symposium 1992. Batavia, Ill: American Heartworm Society,\n          1992:71\u201378."},{"key":"R17","series-title":"J Am Vet Med Assoc","first-page":"693","volume":"216","year":"2000","unstructured":"Snyder PS, Levy JK, Salute ME, et al. Performance of serological tests used to detect heartworm infection in cats.\n          J Am Vet Med Assoc\n          2000;216:693\u2013700."},{"key":"R18","year":"1998","unstructured":"Mondesire RR, Donoghue AR, Krause KA, et al. An enzyme-linked immunosorbent assay for the detection of specific antibodies to Dirofilaria immitis in the cat. In: Proceedings, Heartworm Symposium 1998. Batavia, Ill: American Heartworm Society,\n          1998:51\u201356."},{"key":"R19","series-title":"Vet Parasitol","first-page":"209","volume":"70","year":"1997","unstructured":"Prieto C, Venco L, Simon F, et al. Feline heartworm (Dirofilaria immitis) infection: detection of specific IgG for the diagnosis of occult infections.\n          Vet Parasitol\n          1997;70:209\u2013217."},{"key":"R20","year":"1998","unstructured":"Nelson TC, Young TS. Incidence of Dirofilaria immitus in shelter cats from southeast Texas. In: Proceedings, Heartworm Symposium 1998. Batavia, Ill: American Heartworm Society,\n          1998:63\u201366."},{"key":"R21","year":"1998","unstructured":"McCall JW, Guerrero J, Supakorndej P, et al. Evaluation of the accuracy of heartworm antigen and antibody tests for cats. In: Proceedings, Heartworm Symposium 1998. Batavia, Ill: American Heartworm Society,\n          1998:127\u2013131."},{"key":"R22","year":"1995","unstructured":"McCall JW, Supakorndej N, Ryan W, et al. Utility of an ELISA-based antibody test detection of heartworm infection in cats. In: Proceedings, Heartworm Symposium 1995. Batavia, Ill: American Heartworm Society,\n          1995:127\u2013133."},{"key":"R23","year":"1997","unstructured":"Robertson C, Dillon AR, Brawner W, et al. Clinical study of 215 cats in southeastern US practices. Am Assoc Vet Parisitol,\n          1997:81\u201387."},{"key":"R24","year":"1995","unstructured":"Ryan WG, Gross SJ, Soll MD. Diagnosis of feline heartworm infection. In: Proceedings, Heartworm Symposium 1995. Batavia, Ill: American Heartworm Society,\n          1995:121\u2013126."},{"key":"R25","year":"1995","unstructured":"Dunavent B, Keister MD, Tanner P, et al. Correlation between heart-worm disease classification, serum antigen concentration, and associated clinical pathology values. In: Proceedings, Heartworm Symposium 1995. Batavia, Ill: American Heartworm Society,\n          1995:221\u2013231."},{"key":"R26","year":"1998","unstructured":"Genchi C, Kramer L, Venco L, et al. Comparison of antibody and antigen testing with echocardiography for the detection of heartworm (Dirofilaria immitis) in cats. In: Proceedings, Heartworm Symposium 1998. Batavia, Ill: American Heartworm Society,\n          1998:173\u2013177."},{"key":"R27","series-title":"J Am Anim Hosp Assoc","first-page":"533","volume":"39","year":"2003","unstructured":"Levy JK, Snyder PS, Taveras LM, et al. Prevalence and risk factors for heartworm infection in cats from northern Florida.\n          J Am Anim Hosp Assoc\n          2003;39:533\u2013537."},{"key":"R28","series-title":"J Am Vet Med Assoc","first-page":"91","volume":"213","year":"1998","unstructured":"Smith JW, Scott-Moncrieff JC, Rivers BJ. Pneumothorax secondary to Dirofilaria immitis infection in two cats.\n          J Am Vet Med Assoc\n          1998;213:91\u201393."},{"key":"R29","series-title":"Vet Therapeutics","first-page":"169","volume":"1","year":"2000","unstructured":"Miller MW, Atkins CE, Stemme K, et al. Prevalence of exposure to Dirofilaria immitis in cats in multiple areas of the United States.\n          Vet Therapeutics\n          2000;1:169\u2013175."},{"key":"R30","series-title":"J Am Vet Med Assoc","first-page":"199","volume":"184","year":"1984","unstructured":"Fukushima K, Hutsell D, Patton S, et al. Aberrant dirofilariasis in a cat.\n          J Am Vet Med Assoc\n          1984;184:199\u2013201."},{"key":"R31","year":"2001","unstructured":"Courtney CH. Comparing the performance of heartworm antigen tests in dogs. In: Proceedings, Heartworm Symposium 2001. 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