{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,12]],"date-time":"2026-03-12T15:15:44Z","timestamp":1773328544610,"version":"3.50.1"},"reference-count":24,"publisher":"American Animal Hospital Association","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of the American Animal Hospital Association"],"published-print":{"date-parts":[[2010]]},"abstract":"<jats:p>Three dogs of different breeds, ages, and genders were presented with pale mucous membranes, depression, anorexia, and splenomegaly. Observed were severe normocytic, nor-mochromic, nonregenerative anemia, thrombocytopenia, and leukopenia. Blood smears contained large, atypical cells with blue vacuolated cytoplasm, cytoplasmic blebs, round to oval central nuclei, and elevated numbers of cytoplasmic fragment resembling macroplatelets. Bi- and multinucleated atypical cells were found mainly in spleen, lymph nodes, and bone marrow. A final diagnosis of acute megakaryoblastic leukemia (AMegL) was made based on morphology and positivity to the megakaryocyte-derived cell-specific markers von Willebrand factor and CD61. In case nos. 1 and 2, no treatment was initiated, and the dogs died on days 4 and 3, respectively. Case no. 3 received supportive therapy with prednisone, and after a brief improvement the dog died spontaneously 35 days after initial presentation. Only 11 cases of AMegL have been reported in dogs, and the specific diagnostic criteria have not been well established. The presence of vacuolization, cytoplasmic blebs, central round nuclei, cytoplasmic fragments, and multinucleated cells in these three cases were considered useful to differentiate AMegL from other hematopoietic neoplasms.<\/jats:p>","DOI":"10.5326\/0460327","type":"journal-article","created":{"date-parts":[[2014,9,15]],"date-time":"2014-09-15T12:11:00Z","timestamp":1410783060000},"page":"327-335","source":"Crossref","is-referenced-by-count":29,"title":["Acute Megakaryoblastic Leukemia in Dogs: A Report of Three Cases and Review of the Literature"],"prefix":"10.5326","volume":"46","author":[{"given":"Stefano","family":"Comazzi","sequence":"first","affiliation":[{"name":"Dipartimento di Patologia Animale, Igiene e Sanita\u0300 Pubblica Veterinaria (Comazzi, Gelain, Roccabianca), University of Milan, via Celoria 10, Milan, 20133 Italy"},{"name":"Nerviano Medical Science (Bonfanti), via Pasteur 10, Nerviano, Milan 20014 Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria E.","family":"Gelain","sequence":"additional","affiliation":[{"name":"Dipartimento di Patologia Animale, Igiene e Sanita\u0300 Pubblica Veterinaria (Comazzi, Gelain, Roccabianca), University of Milan, via Celoria 10, Milan, 20133 Italy"},{"name":"Nerviano Medical Science (Bonfanti), via Pasteur 10, Nerviano, Milan 20014 Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ugo","family":"Bonfanti","sequence":"additional","affiliation":[{"name":"Dipartimento di Patologia Animale, Igiene e Sanita\u0300 Pubblica Veterinaria (Comazzi, Gelain, Roccabianca), University of Milan, via Celoria 10, Milan, 20133 Italy"},{"name":"Nerviano Medical Science (Bonfanti), via Pasteur 10, Nerviano, Milan 20014 Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paola","family":"Roccabianca","sequence":"additional","affiliation":[{"name":"Dipartimento di Patologia Animale, Igiene e Sanita\u0300 Pubblica Veterinaria (Comazzi, Gelain, Roccabianca), University of Milan, via Celoria 10, Milan, 20133 Italy"},{"name":"Nerviano Medical Science (Bonfanti), via Pasteur 10, Nerviano, Milan 20014 Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"3280","reference":[{"key":"R1","series-title":"Vet Pathol","first-page":"562","volume":"15","year":"1978","unstructured":"Holscher MA, Collins RD, Glick AD, et al. Megakaryocytic leukemia in a dog.\n          Vet Pathol\n          1978;15:562\u2013565."},{"key":"R2","series-title":"Vet Pathol","first-page":"533","volume":"18","year":"1986","unstructured":"Shull RM, DeNovo RC, McCracken MD. Megakaryoblastic leukemia in a dog.\n          Vet Pathol\n          1986;18:533\u2013536."},{"key":"R3","series-title":"Vet Clin Pathol","first-page":"69","volume":"27","year":"1989","unstructured":"Bolon B, Harvey JW, Kaplan-Stein D. Megakaryoblastic leukemia in a dog.\n          Vet Clin Pathol\n          1989;27:69\u201372."},{"key":"R4","series-title":"Vet Pathol","first-page":"212","volume":"27","year":"1990","unstructured":"Messick J, Carothers M, Wellman M. Identification and characterization of megakaryoblasts in acute megakaryoblastic leukemia in a dog.\n          Vet Pathol\n          1990;27:212\u2013214."},{"key":"R5","series-title":"Vet Pathol","first-page":"186","volume":"30","year":"1993","unstructured":"Colbatzky F, Hermanns W. Acute megakaryoblastic leukemia in one cat and two dogs.\n          Vet Pathol\n          1993;30:186\u2013194."},{"key":"R6","series-title":"J Am Vet Med Assoc","first-page":"194","volume":"207","year":"1995","unstructured":"Pucheu-Haston CM, Camus A, Taboada J, et al. Megakaryoblastic leukemia in a dog.\n          J Am Vet Med Assoc\n          1995;207:194\u2013196."},{"key":"R7","series-title":"J Vet Med Sci","first-page":"177","volume":"58","year":"1996","unstructured":"Miyamoto T, Hachimura H, Amimoto A. A case of megakaryoblastic leukemia in a dog.\n          J Vet Med Sci\n          1996;58:177\u2013179."},{"key":"R8","series-title":"Vet Clin Pathol","first-page":"52","volume":"34","year":"2005","unstructured":"Ledieu D, Palazzi X, Marchal T, et al. Acute megakaryoblastic leukemia with erythrophagocytosis and thrombosis in a dog.\n          Vet Clin Pathol\n          2005;34:52\u201356."},{"key":"R9","series-title":"J Vet Diagn Invest","first-page":"287","volume":"18","year":"2006","unstructured":"Park HM, Doster AR, Tashbaeva RE, et al. Clinical, histopathological and immunohistochemical findings in a case of megakaryoblastic leukemia in a dog.\n          J Vet Diagn Invest\n          2006;18:287\u2013291."},{"key":"R10","series-title":"Vet Clin Pathol","first-page":"288","volume":"36","year":"2007","unstructured":"Suter SE, Vernau W, Fry MM, et al. CD34+, CD41+ acute megakaryoblastic leukemia in a dog.\n          Vet Clin Pathol\n          2007;36: 288\u2013292."},{"key":"R11","series-title":"Vet Clin Pathol","first-page":"63","volume":"20","year":"1991","unstructured":"Jain NC, Blue JT, Grindem CB, et al. Proposed criteria for classification of acute myeloid leukemia in dogs and cats.\n          Vet Clin Pathol\n          1991;20:63\u201382."},{"key":"R12","series-title":"Vet Clin Pathol","first-page":"189","volume":"34","year":"2005","unstructured":"McManus PM. Classification of myeloid neoplasms: a comparative review.\n          Vet Clin Pathol\n          2005;34:189\u2013212."},{"key":"R13","series-title":"Vet Immunol Immunopathol","first-page":"145","volume":"69","year":"1999","unstructured":"Vernau W, Moore PF. An immunophenotypic study of canine leukemias and preliminary assessment of clonality by polymerase chain reaction.\n          Vet Immunol Immunopathol\n          1999;69:145\u2013164."},{"key":"R14","series-title":"Blood","first-page":"880","volume":"107","year":"2006","unstructured":"Oki Y, Kantarjian HM, Zhou X, et al. Adult acute megakaryocytic leukemia: an analysis of 37 patients treated at M.D. Abderson Cancer Center.\n          Blood\n          2006;107:880\u2013884."},{"key":"R15","series-title":"Leukemia","first-page":"1622","volume":"16","year":"2002","unstructured":"Pagano L, Pulsoni A, Vignetti M, et al. Acute megakaryoblastic leukemia: experience of GIMEMA trials.\n          Leukemia\n          2002;16: 1622\u20131626."},{"key":"R16","series-title":"Pediatr Hematol Oncol","first-page":"211","volume":"4","year":"1987","unstructured":"Zipursky A, Peeters M, Poon A. Megakaryoblastic leukemia and Down\u2019s syndrome: a review.\n          Pediatr Hematol Oncol\n          1987;4:211\u2013230."},{"key":"R17","series-title":"Leuk Lymphoma","first-page":"69","volume":"18","year":"1995","unstructured":"Gassmann W, Loffler H. Acute megakaryoblastic leukemia.\n          Leuk Lymphoma\n          1995;18:69\u201373."},{"key":"R18","series-title":"Vet Clin Pathol","first-page":"47","volume":"35","year":"2006","unstructured":"Comazzi S, Gelain ME, Spagnolo V, et al. Cytometric patterns of blood from dogs with neoplastic and non-neoplastic diseases using double labeling for CD18\/45.\n          Vet Clin Pathol\n          2006;35:47\u201354."},{"key":"R19","series-title":"Vet Pathol","first-page":"204","volume":"33","year":"1996","unstructured":"Caniatti M, Roccabianca P, Scanziani E, et al. Canine lymphoma: immunocytochemical analysis of fine needle-aspiration biopsy.\n          Vet Pathol\n          1996;33:204\u2013212."},{"key":"R20","series-title":"Vet Clin Pathol","first-page":"55","volume":"35","year":"2006","unstructured":"Villiers E, Baines S, Law AM, et al. Identification of acute myeloid leukemia in dogs using flow cytometry with myeloperoxidase, MAC387, and a canine neutrophil-specific antibody.\n          Vet Clin Pathol\n          2006;35:55\u201371."},{"key":"R21","series-title":"Vet Clin Pathol","first-page":"126","volume":"38","year":"2009","unstructured":"Rossi S, Gelain ME, Comazzi S. Disseminated histiocytic sarcoma with peripheral blood involvement in a bernese mountain dog.\n          Vet Clin Pathol\n          2009;38:126\u2013130."},{"key":"R22","series-title":"Vet Clin Pathol","first-page":"190","volume":"37","year":"2008","unstructured":"Allison RW, Brunker JD, Breshears MA, et al. Dendritic cell leukemia in a golden retriever.\n          Vet Clin Pathol\n          2008;37:190\u2013197."},{"key":"R23","series-title":"Vet Immunol Immunopathol","first-page":"243","volume":"112","year":"2006","unstructured":"Comazzi S, Gelain ME, Riondato F, et al. Flow cytometric expression of common antigens CD18\/CD45 in blood from dogs with lymphoid malignancies: a semi-quantitative study.\n          Vet Immunol Immunopathol\n          2006;112:243\u2013252."},{"key":"R24","series-title":"J Biol Regul Homeost Ag","first-page":"68","volume":"15","year":"2001","unstructured":"Basso G, Lanza F, Orfao A, et al. Clinical and biological significance of CD34 expression in acute leukemia.\n          J Biol Regul Homeost Ag\n          2001;15:68\u201378."}],"container-title":["Journal of the American Animal Hospital Association"],"original-title":[],"link":[{"URL":"https:\/\/jaaha.kglmeridian.com\/view\/journals\/aaha\/46\/5\/article-p327.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/meridian.allenpress.com\/jaaha\/article-pdf\/46\/5\/327\/1330809\/0460327.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/jaaha.kglmeridian.com\/downloadpdf\/journals\/aaha\/46\/5\/article-p327.xml","content-type":"text\/html","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/jaaha.kglmeridian.com\/downloadpdf\/journals\/aaha\/46\/5\/article-p327.xml","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,11,21]],"date-time":"2025-11-21T15:56:32Z","timestamp":1763740592000},"score":1,"resource":{"primary":{"URL":"https:\/\/jaaha.kglmeridian.com\/view\/journals\/aaha\/46\/5\/article-p327.xml"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010]]},"references-count":24,"journal-issue":{"issue":"5"},"URL":"https:\/\/doi.org\/10.5326\/0460327","relation":{},"ISSN":["0587-2871","1547-3317"],"issn-type":[{"value":"0587-2871","type":"print"},{"value":"1547-3317","type":"electronic"}],"subject":[],"published":{"date-parts":[[2010]]}}}