{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,14]],"date-time":"2026-02-14T09:12:19Z","timestamp":1771060339124,"version":"3.50.1"},"reference-count":14,"publisher":"American Animal Hospital Association","issue":"4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2017]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Cutaneous cytologic sampling techniques are used to detect bacteria, yeast, and inflammatory cells for diagnosis and therapeutic monitoring. Studies have examined slide evaluation techniques, ear swab cytology staining methods, and observer variations; few studies compare common clinical sampling techniques. The primary aim of this study was to measure detection of microorganisms and neutrophils by impression smear compared to acetate tape preparation; comparison of agreement between two acetate tape staining methods was a secondary aim. Thirty lesions consistent with superficial pyoderma were sampled via impression smear and acetate tape preparation. Acetate tape preparations were either stained with modified Romanowksy stain solutions two and three or solution three alone. Impression smears were stained in the standard manner. Bacteria, yeast, and neutrophils were evaluated using a semi-quantitative scale [0-4]. Quantities were aggregated and compared using Cohen's kappa to measure agreement between methods. When impression smears were compared to acetate tape, the lowest agreement occurred for neutrophils, with impression smears detecting more neutrophils. Comparison of acetate tape staining methods had the highest agreement for yeast detection. Sampling technique and staining method did not differ for detection of bacteria. Impression smears detected more neutrophils, and yeast detection appeared equivalent for acetate tape staining methods.<\/jats:p>","DOI":"10.5326\/jaaha-ms-6458","type":"journal-article","created":{"date-parts":[[2017,5,23]],"date-time":"2017-05-23T15:24:18Z","timestamp":1495553058000},"page":"193-197","source":"Crossref","is-referenced-by-count":6,"title":["Impression Smear Agreement with Acetate Tape Preparation for Cytologic Sampling"],"prefix":"10.5326","volume":"53","author":[{"given":"Elizabeth A.","family":"Layne","sequence":"first","affiliation":[{"name":"From the Department of Medical Sciences, School of Veterinary Medicine, University of Wisconsin-Madison, Madison, Wisconsin (E.A.L.); and the Department of Small Animal Medicine, College of Veterinary Medicine, University of Georgia, Athens, Georgia (S.Z.)."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sonja","family":"Zabel","sequence":"additional","affiliation":[{"name":"S. Zabel's present affiliation is the VCA Northwest Veterinary Specialists, Portland, Oregon."},{"name":"From the Department of Medical Sciences, School of Veterinary Medicine, University of Wisconsin-Madison, Madison, Wisconsin (E.A.L.); and the Department of Small Animal Medicine, College of Veterinary Medicine, University of Georgia, Athens, Georgia (S.Z.)."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"3280","reference":[{"key":"i1547-3317-53-4-193-b01","series-title":"Clin Tech Small Anim Pract","first-page":"117","article-title":"Practical cytology for inflammatory skin diseases","volume":"21","author":"Mendelsohn \n              C,","year":"2006","unstructured":"Mendelsohn \n              C, \n            \n            \n              Rosenkrantz \n              W, \n            \n            \n              Griffin \n              CE. \n            \n          \n          Practical cytology for inflammatory skin diseases. Clin Tech Small Anim Pract2006;21:117\u201327."},{"key":"i1547-3317-53-4-193-b02","series-title":"Muller and Kirk's Small Animal Dermatology. 7th ed","first-page":"81","author":"Miller \n              WH,","year":"2013","unstructured":"Miller \n              WH, \n            \n            \n              Griffin \n              CE, \n            \n            \n              Campbell \n              KL. \n            \n          \n          Muller and Kirk's Small Animal Dermatology. 7th ed. St. Louis: Elsevier Mosby;2013:81\u20136."},{"key":"i1547-3317-53-4-193-b03","series-title":"Vet Clin Small Anim","first-page":"1","article-title":"Recognizing Pyoderma","volume":"43","author":"Gortel \n              K.","year":"2013","unstructured":"Gortel \n              K. \n            \n          \n          Recognizing Pyoderma. Vet Clin Small Anim2013;43:1\u201318."},{"key":"i1547-3317-53-4-193-b04","series-title":"Small Animal Dermatology: a color atlas and therapeutic guide. 2nd ed","first-page":"23","author":"Hnilica \n              KA.","year":"2011","unstructured":"Hnilica \n              KA. \n            \n          \n          Small Animal Dermatology: a color atlas and therapeutic guide. 2nd ed. St. Louis: Elsevier Saunders;2011:23\u20136."},{"key":"i1547-3317-53-4-193-b05","series-title":"Clinical Companion: Small Animal Dermatology. 2nd ed","first-page":"20","author":"Helton-Rhodes \n              K,","year":"2011","unstructured":"Helton-Rhodes \n              K, \n            \n            \n              Werner \n              A. \n            \n          \n          Clinical Companion: Small Animal Dermatology. 2nd ed. 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Vet Dermatol2002;13:151\u20136."},{"key":"i1547-3317-53-4-193-b14","series-title":"Statistical methods for the analysis of biomedical data. 1st ed","first-page":"255","author":"Woolson \n              RF.","year":"1987","unstructured":"Woolson \n              RF. \n            \n          \n          Statistical methods for the analysis of biomedical data. 1st ed. New York: Wiley;1987:255\u20137."}],"container-title":["Journal of the American Animal Hospital Association"],"original-title":[],"link":[{"URL":"https:\/\/jaaha.kglmeridian.com\/view\/journals\/aaha\/53\/4\/article-p193.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/meridian.allenpress.com\/jaaha\/article-pdf\/53\/4\/193\/1329332\/jaaha-ms-6458.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/jaaha.kglmeridian.com\/downloadpdf\/journals\/aaha\/53\/4\/article-p193.xml","content-type":"text\/html","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/jaaha.kglmeridian.com\/downloadpdf\/journals\/aaha\/53\/4\/article-p193.xml","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,11,21]],"date-time":"2025-11-21T16:02:30Z","timestamp":1763740950000},"score":1,"resource":{"primary":{"URL":"https:\/\/jaaha.kglmeridian.com\/view\/journals\/aaha\/53\/4\/article-p193.xml"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017]]},"references-count":14,"journal-issue":{"issue":"4"},"URL":"https:\/\/doi.org\/10.5326\/jaaha-ms-6458","relation":{},"ISSN":["0587-2871","1547-3317"],"issn-type":[{"value":"0587-2871","type":"print"},{"value":"1547-3317","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017]]}}}