{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T19:45:35Z","timestamp":1791315935824,"version":"4.3.1"},"reference-count":24,"publisher":"American Animal Hospital Association","issue":"4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>The purpose of this study was to determine the correlation between cytology and histology in the diagnosis of canine tympanokeratoma. Dogs that had a middle ear aspiration cytology via otoendoscope-guided myringotomy and a histology of the tympanic bulla content sampled following total ear canal ablation with lateral bulla osteotomy between 2010 and 2024 in one hospital were retrospectively examined. Cytological diagnosis of tympanokeratoma was considered when the sample contained a majority of corneocytes. Thirty-seven ears with a pathological diagnosis of either tympanokeratoma (31\/37) or nontympanokeratoma otitis media (6\/37) were chosen. Cytology had an accuracy of 78%, a sensitivity of 74%, and a specificity of 100% for diagnosing tympanokeratoma, with negative and positive predictive values of 43 and 100%, respectively. Among ears with histologically confirmed tympanokeratoma, imaging showed lysis and expansion of the tympanic bulla in 45.2 and 12.9%, respectively. Otoendoscopy showed a mass in the horizontal ear canal in 22.6%. Dense opaque pearly yellowish material was sampled from the tympanic cavity in 45.2%. Middle ear aspiration cytology via otoendoscope-guided myringotomy is a useful tool for diagnosing tympanokeratoma and is highly reliable in cases of positive outcomes. However, its capacity to rule out the condition is limited, particularly in infected ears.<\/jats:p>","DOI":"10.5326\/jaaha-ms-7558","type":"journal-article","created":{"date-parts":[[2026,7,11]],"date-time":"2026-07-11T00:30:53Z","timestamp":1783729853000},"page":"169-175","source":"Crossref","is-referenced-by-count":2,"title":["Middle Ear Aspiration Cytology via Otoendoscope-Guided Myringotomy Reliably Confirms Tympanokeratoma in Dogs"],"prefix":"10.5326","volume":"62","author":[{"given":"Estelle","family":"Ferenczi","sequence":"first","affiliation":[{"name":"From the Surgery Unit EVOLIA Veterinary Clinic, L\u2019Isle Adam, France (E.F.);"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrea","family":"Deleani","sequence":"additional","affiliation":[{"name":"Surgery Unit Anicura-ADVETIA Veterinary Referral Hospital, V\u00e9lizy-Villacoublay, France (A.D., D.R.); and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amaury","family":"Briand","sequence":"additional","affiliation":[{"name":"Dermatology Unit Anicura-ADVETIA Veterinary Referral Hospital, V\u00e9lizy-Villacoublay, France (A.B., M.M.-A., P.P.)."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M\u00e9lanie","family":"Moreira-Andr\u00e9","sequence":"additional","affiliation":[{"name":"Dermatology Unit Anicura-ADVETIA Veterinary Referral Hospital, V\u00e9lizy-Villacoublay, France (A.B., M.M.-A., P.P.)."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pascal","family":"Prelaud","sequence":"additional","affiliation":[{"name":"Dermatology Unit Anicura-ADVETIA Veterinary Referral Hospital, V\u00e9lizy-Villacoublay, France (A.B., M.M.-A., P.P.)."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Diego","family":"Rossetti","sequence":"additional","affiliation":[{"name":"Surgery Unit Anicura-ADVETIA Veterinary Referral Hospital, V\u00e9lizy-Villacoublay, France (A.D., D.R.); and"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"3280","reference":[{"issue":"3","key":"i1547-3317-62-4-169-b1","series-title":"Vet J","first-page":"440","article-title":"Canine aural cholesteatoma: a histological and immunohistochemical study","volume":"200","author":"Banco","year":"2014","unstructured":"Banco B, Grieco V, Di Giancamillo M,  Canine aural cholesteatoma: a histological and immunohistochemical study. Vet J 2014;200(3):440\u20135."},{"issue":"6","key":"i1547-3317-62-4-169-b2","series-title":"Otolaryngol Clin North Am","first-page":"1143","article-title":"The pathophysiology of cholesteatoma","volume":"39","author":"Semaan","year":"2006","unstructured":"Semaan MT, Megerian CA. The pathophysiology of cholesteatoma. Otolaryngol Clin North Am 2006;39(6):1143\u201359."},{"issue":"4","key":"i1547-3317-62-4-169-b3","series-title":"Vet Clin North Am Small Anim Pract","first-page":"623","article-title":"Diagnosis and management of cholesteatomas in dogs","volume":"46","author":"Risselada","year":"2016","unstructured":"Risselada M. Diagnosis and management of cholesteatomas in dogs. Vet Clin North Am Small Anim Pract 2016;46(4):623\u201334."},{"key":"i1547-3317-62-4-169-b4","series-title":"Vet Dermatol","first-page":"1","article-title":"Aural cholesteatoma (tympanokeratoma) in veterinary medicine: an update and principles of clinical management","author":"Berger","year":"2026","unstructured":"Berger AC, Bertran J, Williams RW,  Aural cholesteatoma (tympanokeratoma) in veterinary medicine: an update and principles of clinical management. Vet Dermatol 2026:1\u201318."},{"issue":"22","key":"i1547-3317-62-4-169-b5","series-title":"J Clin Med","first-page":"6791","article-title":"Comprehensive management of cholesteatoma in otitis media: diagnostic challenges, imaging advances, and surgical outcome","volume":"13","author":"Popescu","year":"2024","unstructured":"Popescu C, V\u0103ru\u021b RM, Puticiu M,  Comprehensive management of cholesteatoma in otitis media: diagnostic challenges, imaging advances, and surgical outcome. J Clin Med 2024;13(22):6791."},{"issue":"4","key":"i1547-3317-62-4-169-b6","series-title":"Vet Dermatol","first-page":"417","article-title":"Clinical aspects of middle ear tympanokeratoma in dogs diagnosed through advanced imaging, otoendoscopy and histopathological evaluation","volume":"36","author":"Botelho","year":"2025","unstructured":"Botelho CB, Tortoriello R, Koch SN,  Clinical aspects of middle ear tympanokeratoma in dogs diagnosed through advanced imaging, otoendoscopy and histopathological evaluation. Vet Dermatol 2025;36(4):417\u201323."},{"issue":"6","key":"i1547-3317-62-4-169-b7","series-title":"Vet Radiol Ultrasound","first-page":"836","article-title":"Characteristics and diagnostic performance of nonechoplanar diffusion-weighted imaging in detecting canine tympanokeratoma (cholesteatoma)","volume":"65","author":"Coeuriot","year":"2024","unstructured":"Coeuriot C, Gaillot H, Agoulon A,  Characteristics and diagnostic performance of nonechoplanar diffusion-weighted imaging in detecting canine tympanokeratoma (cholesteatoma). Vet Radiol Ultrasound 2024;65(6):836\u201343."},{"issue":"1","key":"i1547-3317-62-4-169-b8","series-title":"Vet Dermatol","first-page":"42-e12","article-title":"Clinical analysis and nonsurgical management of 11 dogs with aural cholesteatoma","volume":"30","author":"Imai","year":"2019","unstructured":"Imai A, Kondo H, Suganuma T,  Clinical analysis and nonsurgical management of 11 dogs with aural cholesteatoma. Vet Dermatol 2019;30(1):42-e12."},{"issue":"8","key":"i1547-3317-62-4-169-b9","series-title":"Vet Surg","first-page":"763","article-title":"Aural cholesteatoma in twenty dogs","volume":"37","author":"Hardie","year":"2008","unstructured":"Hardie EM, Linder KE, Pease AP. Aural cholesteatoma in twenty dogs. Vet Surg 2008;37(8):763\u201370."},{"issue":"6","key":"i1547-3317-62-4-169-b10","series-title":"Can Vet J","first-page":"631","article-title":"Middle ear cholesteatoma in 11 dogs","volume":"52","author":"Greci","year":"2011","unstructured":"Greci V, Travetti O, Di Giancamillo M,  Middle ear cholesteatoma in 11 dogs. Can Vet J 2011;52(6):631\u20136."},{"issue":"1","key":"i1547-3317-62-4-169-b11","series-title":"Vet Clin Pathol","first-page":"157","article-title":"Cholesteatoma and meningoencephalitis in a dog with chronic otitis externa","volume":"44","author":"Newman","year":"2015","unstructured":"Newman AW, Estey CM, McDonough S,  Cholesteatoma and meningoencephalitis in a dog with chronic otitis externa. Vet Clin Pathol 2015;44(1):157\u201363."},{"issue":"3","key":"i1547-3317-62-4-169-b12","series-title":"J Small Anim Pract","first-page":"188","article-title":"Cholesterol granuloma as long-term complication of total ear canal ablation in a dog","volume":"53","author":"Riedinger","year":"2012","unstructured":"Riedinger B, Albaric O, Gauthier O. Cholesterol granuloma as long-term complication of total ear canal ablation in a dog. J Small Anim Pract 2012;53(3):188\u201391."},{"issue":"4","key":"i1547-3317-62-4-169-b13","series-title":"J Am Anim Hosp Assoc","first-page":"261","article-title":"Cholesteatoma after lateral bulla osteotomy in two brachycephalic dogs","volume":"48","author":"Schuenemann","year":"2012","unstructured":"Schuenemann RM, Oechtering G. Cholesteatoma after lateral bulla osteotomy in two brachycephalic dogs. J Am Anim Hosp Assoc 2012;48(4):261\u20138."},{"issue":"1","key":"i1547-3317-62-4-169-b14","series-title":"Vet Record Case Rep","first-page":"e561","article-title":"Long-term non-surgical management of canine aural tympanokeratoma with repeated removal of middle ear keratinous debris and CT follow-up","volume":"11","author":"Vanham\u00e4ki","year":"2023","unstructured":"Vanham\u00e4ki L, Sepp\u00e4nen R, Candido M,  Long-term non-surgical management of canine aural tympanokeratoma with repeated removal of middle ear keratinous debris and CT follow-up. Vet Record Case Rep 2023;11(1):e561."},{"issue":"9","key":"i1547-3317-62-4-169-b15","series-title":"J Feline Med Surg","first-page":"1098612X241275286","article-title":"Clinical outcome of cats with suppurative otitis media and intact tympanum submitted to myringotomy: retrospective findings from 26 cases","volume":"26","author":"Deleporte","year":"2024","unstructured":"Deleporte S, Briand A, Prelaud P. Clinical outcome of cats with suppurative otitis media and intact tympanum submitted to myringotomy: retrospective findings from 26 cases. J Feline Med Surg 2024;26(9):1098612X241275286."},{"issue":"5","key":"i1547-3317-62-4-169-b16","series-title":"Vet Surg","first-page":"263","article-title":"Total ear canal ablation combined with lateral bulla osteotomy for end-stage otitis in dogs. Results in thirty dogs","volume":"17","author":"Mason","year":"1988","unstructured":"Mason LK, Harvey CE, Orsher RJ. Total ear canal ablation combined with lateral bulla osteotomy for end-stage otitis in dogs. Results in thirty dogs. Vet Surg 1988;17(5):263\u20138."},{"issue":"11","key":"i1547-3317-62-4-169-b17","series-title":"J Small Anim Pract","first-page":"547","article-title":"Total ear canal ablation and lateral bulla osteotomy in the dog","volume":"31","author":"White","year":"1990","unstructured":"White RaS, Pomeroy CJ. Total ear canal ablation and lateral bulla osteotomy in the dog. J Small Anim Pract 1990;31(11):547\u201353."},{"issue":"1","key":"i1547-3317-62-4-169-b18","series-title":"JAVMA","first-page":"84","article-title":"Total ear canal ablation combining bulla osteotomy and curettage in dogs with chronic otitis externa and media","volume":"196","author":"Beckman","year":"1990","unstructured":"Beckman SL, Henry WB, Cechner P. Total ear canal ablation combining bulla osteotomy and curettage in dogs with chronic otitis externa and media. JAVMA 1990;196(1):84\u201390."},{"issue":"3","key":"i1547-3317-62-4-169-b19","series-title":"J Am Anim Hosp Assoc","first-page":"257","article-title":"Total ear canal ablation and lateral bulla osteotomy in 38 dogs","volume":"26","author":"Matthiesen","year":"1990","unstructured":"Matthiesen DT, Scavelli T. Total ear canal ablation and lateral bulla osteotomy in 38 dogs. J Am Anim Hosp Assoc 1990;26(3):257\u201367."},{"issue":"4","key":"i1547-3317-62-4-169-b20","series-title":"Vet Radiol Ultrasound","first-page":"374","article-title":"Computed tomography features of middle ear cholesteatoma in dogs","volume":"51","author":"Travetti","year":"2010","unstructured":"Travetti O, Giudice C, Greci V,  Computed tomography features of middle ear cholesteatoma in dogs. 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