{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,27]],"date-time":"2026-02-27T08:21:53Z","timestamp":1772180513438,"version":"3.50.1"},"reference-count":33,"publisher":"Korean Academy of Conservative Dentistry","issue":"1","license":[{"start":{"date-parts":[[2026,2,13]],"date-time":"2026-02-13T00:00:00Z","timestamp":1770940800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/3.0\/"}],"content-domain":{"domain":["rde.ac"],"crossmark-restriction":true},"short-container-title":["Restor Dent Endod"],"published-print":{"date-parts":[[2026,2,28]]},"abstract":"<jats:p>Objectives: This study aimed to assess the expression of neuropeptide Y (NPY) in human dental pulp after tooth bleaching with three in-office hydrogen peroxide (H&lt;sub&gt;2&lt;\/sub&gt;O&lt;sub&gt;2&lt;\/sub&gt;)-based systems.Methods: Forty pulps were collected from premolars scheduled for extraction and divided into four groups (&lt;i&gt;n&lt;\/i&gt; = 10): Control (no bleaching; basal NPY values); Pola Office (35% H&lt;sub&gt;2&lt;\/sub&gt;O&lt;sub&gt;2&lt;\/sub&gt;, 8 minutes); Opalescence Boost (40% H&lt;sub&gt;2&lt;\/sub&gt;O&lt;sub&gt;2&lt;\/sub&gt;, 20 minutes); and Zoom (25% H&lt;sub&gt;2&lt;\/sub&gt;O&lt;sub&gt;2&lt;\/sub&gt; + cold blue light, 15 minutes). After extraction, pulps were fixed in 4% formaldehyde and processed. NPY levels were quantified using enzyme-linked immunosorbent assay. Data distribution was assessed with the Shapiro-Wilk test. One-way analysis of variance and Tukey &lt;i&gt;post-hoc&lt;\/i&gt; test with Bonferroni correction were applied (&lt;i&gt;p&lt;\/i&gt; &lt; 0.05).Results: NPY expression differed significantly among groups (&lt;i&gt;p&lt;\/i&gt; = 0.0097). The control group showed the lowest mean expression (0.026 \u00b1 0.002 pmol\/mg of pulp tissue), followed by Zoom (0.031 \u00b1 0.005 pmol\/mg), Pola Office (0.040 \u00b1 0.004 pmol\/mg), and Opalescence Boost, which exhibited the highest NPY expression (0.044 \u00b1 0.004 pmol\/mg). Post-hoc analysis revealed a statistically significant difference between the control and Opalescence Boost groups (&lt;i&gt;p&lt;\/i&gt; = 0.0122).Conclusions: The increase in NPY expression\u2014particularly with Opalescence Boost\u2014indicates that in-office bleaching agents can elicit measurable neurobiological responses in pulp tissue after a single application. The significant difference between the control and Opalescence Boost groups suggests a possible H&lt;sub&gt;2&lt;\/sub&gt;O&lt;sub&gt;2&lt;\/sub&gt; concentration- or formulation-dependent effect on pulpal neuropeptide activity, underscoring the need for further research on the biological impact of bleaching treatments.<\/jats:p>","DOI":"10.5395\/rde.2026.51.e10","type":"journal-article","created":{"date-parts":[[2026,2,13]],"date-time":"2026-02-13T04:14:33Z","timestamp":1770956073000},"page":"e10","update-policy":"https:\/\/doi.org\/10.5395\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Neuropeptide Y regulation of dental pulp neurogenic inflammation provoked by tooth bleaching agents: a descriptive comparative clinical 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